生物标志物检测法评价注射用丹参多酚酸对血小板活性的影响
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摘要
目的:采用生物标志物检测法评价注射用丹参多酚酸对血小板活性的影响。方法:用检测生物标志物(血小板膜糖蛋白CD62p)的方法测定经二磷酸腺苷(ADP)激活的血小板活化率,判定受试物是否具有抑制血小板活化的作用。体内法比较大鼠给予受试物后和给药前抗凝血的血小板活化率,体外法比较添加受试物与未加受试物混合的大鼠抗凝血的血小板活化率。结果:在体内和体外模型中,注射用丹参多酚酸可明显抑制血小板上由ADP激活的CD62p高表达,血小板的活化受到抑制。结论:采用生物标志物检测的方法,简单快捷,可有效的评价药物对血小板活性的影响,体外法更适合建立检测标准。
Objective: To evaluate the effect of salvianolic acid injection onplatelet activation by biomarker test. Methods: The activation rate of the platelets activated by adenosine diphosphate was determined by biomarker(platelet membrance glycoproteins CD62 p) test, and the inhibition effect on platelet activation of test sample was judged. The variation of the platelet activation rates with dosing of test drug was compared by the rats in vivo method and rats anticoagulant blood in vitro method. Result:Salvianolic acid injection can obviously reduce the expression of CD62 p caused by ADP, which leads to inhibit platelet activation in vivo an in vitro. Conclusion: The biomarker test method is simple and rapid, and can evaluate the effect of drug on platelet activity effectively. The in vitro method is more appropriate to establish the test criteria.
Objective: To evaluate the effect of salvianolic acid injection onplatelet activation by biomarker test. Methods: The activation rate of the platelets activated by adenosine diphosphate was determined by biomarker(platelet membrance glycoproteins CD62 p) test, and the inhibition effect on platelet activation of test sample was judged. The variation of the platelet activation rates with dosing of test drug was compared by the rats in vivo method and rats anticoagulant blood in vitro method. Result:Salvianolic acid injection can obviously reduce the expression of CD62 p caused by ADP, which leads to inhibit platelet activation in vivo an in vitro. Conclusion: The biomarker test method is simple and rapid, and can evaluate the effect of drug on platelet activity effectively. The in vitro method is more appropriate to establish the test criteria.
引文
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[2] 王凤琴,陈岑,夏之宁,等.血小板在活血化瘀中药研究中的应用[J].中国中药杂志,2014,39(16):2993WANG FQ,CHEN C,XIA ZN,et al.Application of platelets in studies on traditional Chinese medicines promoting blood circulation to remove blood stasis[J].China J Chin Mater Med,2014,39(16):2993
[3] 谭大伟,朱平,李小鹰.氯吡格雷抑制血小板功能的两种检测方法比较:血小板聚集实验与血小板膜糖蛋白检测[J].中国组织工程研究与临床康复,2007,11(11):2021TAN DW,ZHU P,LI XY.Effect of clopidogrel on platelet function:Platelet aggregation experiment versus platelet membrane glycoprotein determination[J].J Clin Rehabil Tissue Eng Res,2007;11(11):2021
[4] 张文超,韦建瑞,蒋作锋,等.流式细胞术检测阿司匹林对冠心病患者血小板活化标志物的影响[J].中国医院药学杂志,2007,27(2):164ZHANG WC,WEI JR,JIANG ZF,et al.Effect of aspirin on platelet activation markers in patients with coronary heart disease by FCM[J].Chin Hosp Pharm J,2007,27(2):164
[5] 张倩,陈岑,杨丰庆,等.激动剂诱导血小板活化机制及抗血小板药物研究进展[J].中国药科大学学报,2014,45(6):632ZHANG Q,CHEN C,YANG FQ,et al.Mechanism of agonist-induced platelet activation and research progress of anti-platelet drugs[J].J China Pharm Univ,2014,45(6):632
[6] 李祖兰,白洁,秦晓玲,等.不同抗凝剂对血小板聚集和血小板膜糖蛋白的影响[J].军医进修学院学报,2010,31(7):699LI ZL,BAI J,QIN XL,et al.Effect of anticoagulants on aggregation and membrane glycoprotein of platelets[J].J Chin PLA Postgrad Med Sch,2010,31(7):699
[7] 邵良,季美华,陶国华,等.进展性卒中患者血小板膜糖蛋白CD41和CD61及CD62P的表达[J].中国脑血管病杂志,2007,4(4):156SHAO L,JIMH,TAO GH,et al.The expression of plateletmembrane glycoproteins CD41,CD61 and CD62P in patients with progressive stroke[J].Chin J Cerebrovasc Dis 2007,4(4):156
[8] 陈可冀.血瘀证与活血化瘀治疗的研究[J].中国中医药现代远程教育,2005,11(3):10CHEN KJ.Clinical research of syndrome of blood stasis and promoting blood circulation to remove blood stasis[J].Chin Med Mod Distance Educat China,2005,11(3):10