非小细胞肺癌中E-cadherin、MUC1及PINCH的表达及其临床意义
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摘要
目的:分析与细胞黏附相关的E-cadherin、MUC1及PINCH在非小细胞肺癌(NSCLC)中表达水平,并探讨三者之间的相关性,探寻NSCLC病变进展更具代表性的指标。方法:通过免疫组化法检测70例非小细胞肺癌患者E-cadherin、MUC1及PINCH的表达情况并结合临床分期及有无淋巴结转移进行统计学分析,并探讨三者之间的相关性。结果:NSCLC肿瘤组织中E-cadherin、MUC1及PINCH蛋白的阳性表达率分别是25. 7%、57. 1%和62. 9%,与对照组相比差异具有统计学意义(P<0. 01); E-cadherin阳性表达率随着临床分期的升高而下降(P<0. 01),而MUC1和PINCH阳性表达率随着分期的升高而升高(P<0. 01);有淋巴结转移的肺癌组织中E-cadherin阳性表达率较无淋巴结转移者显著降低(P<0. 05),而有淋巴结转移的肺癌组织中MUC1和PINCH阳性表达率较无淋巴结转移者显著升高(P<0. 05); NSCLC肿瘤组织中MUC1与PINCH的表达水平呈正相关(r=0. 589,P<0. 01),MUC1、PINCH的表达水平与E-cadherin呈负相关(相关系数r值分别为-0. 562,-0. 415,P值均<0. 01)。结论:E-cadherin,MUC1及PINCH三者可能互相联系共同影响细胞间的黏附性进而促进NSCLC的进展;同时检测三者在NSCLC癌组织中的表达,对于探寻NSCLC病变进展更具代表性的指标可能有重要意义。
Objective: To examine the expression of E-cadherin,Mucins1( MUC1) and PINCH in non-small cell lung cancer( NSCLC),and to analyze their correlation. Methods: The expression of E-cadherin Mucins1( MUC1) and PINCH were detected by in70 primary NSCLC samples by immunohistochemistry method,and their correlation then analyzed. Results: The expression of E-cadherin( 25. 7%) was remarkably decreased in NSCLC tissues,while the expression of MUC1 and PINCH( 57. 1% and 62. 9% respectively) was remarkably increased( P<0. 01). E-cadherin,MUC1 and PINCH expression in NSCLC were related to clinical stage and lymphatic metastasis( P<0. 05). The expression of MUC1 and PINCH were positively correlated with the expression of MUC1 and PINCH( r=0. 589,P<0. 01),and E-cadherin was negative correlation with them( r was-0. 562 and-0. 415 respectively,P<0. 01). Conclusion: E-cadherin,MUC1 and PINCH may interrelated mutually on cell-adhesion,and further promote the progress of NSCLC. Detecting this three protein in NSCLC cancer,may have important significance for the invasion and metastasis in NSCLC.
Objective: To examine the expression of E-cadherin,Mucins1( MUC1) and PINCH in non-small cell lung cancer( NSCLC),and to analyze their correlation. Methods: The expression of E-cadherin Mucins1( MUC1) and PINCH were detected by in70 primary NSCLC samples by immunohistochemistry method,and their correlation then analyzed. Results: The expression of E-cadherin( 25. 7%) was remarkably decreased in NSCLC tissues,while the expression of MUC1 and PINCH( 57. 1% and 62. 9% respectively) was remarkably increased( P<0. 01). E-cadherin,MUC1 and PINCH expression in NSCLC were related to clinical stage and lymphatic metastasis( P<0. 05). The expression of MUC1 and PINCH were positively correlated with the expression of MUC1 and PINCH( r=0. 589,P<0. 01),and E-cadherin was negative correlation with them( r was-0. 562 and-0. 415 respectively,P<0. 01). Conclusion: E-cadherin,MUC1 and PINCH may interrelated mutually on cell-adhesion,and further promote the progress of NSCLC. Detecting this three protein in NSCLC cancer,may have important significance for the invasion and metastasis in NSCLC.
引文
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[15]Kuemmel A,Single K,Bittinger F,et al.TA-MUC1 epitope in non-small cell lung cancer[J].Lung Cancer,2009,63(1)98-105.
[16]Wang-Rodriguez J,Dreilinger AD,Alsharabi GM,et al.The signaling adapter protein PINCH is up-regulated in the stroma of common cancer,notably at invasive edges[J].Cancer,2002,95(6):1387-1395.
[17]Zhang JT,Li QX,Wang D,et al.Up-regulation of PINCH in the stroma of oral squamous cell carcinoma predicts nodal metastasis[J].Oncol Rep,2005,14(6):1519-1522.
[18]魏路军任新友,徐林浩,等.E-cadherin、MUC1及PTEN与肿瘤转移相关性[J].青岛大学医学院学报,2010,46(2):183-185.Wei LJ,Ren XY,Xu LH,et al.Correlation of E-cadherin,MUC1and PTEN with tumor metastasis[J].Acta Acad Med Qingdao Universitatis,2010,46(2):183-185.[收稿]
[2]Siegel RL,Miller KD,Jemal A.Cancer statistics,2017[J].CACancer J Clin,2017,67(1):7-30.
[3]Chen L,Jian W,Lu L,et al.Elevated expression of E-cadherin in primary breast cancer and its corresponding metastatic lymph node.[J].Int J Clin Exp Med,2015,8(7):11752-11758.
[4]Baumgart E,Cohen MS,Silva Neto B,et al.Identification and prognostic significance of an epithelial-mesenchymal transition expression profile in human bladder tumors[J].Clin Cancer Res,2007,13(6):1685-1694.
[5]Nagathihalli NS,Merchant NB.Src-mediated regulation of E-cadherin and EMT in pancreatic cancer.[J].Front Biosci,2012,17(3):2059-2069.
[6]Qiu ZX,Zhao S,Li L1,et al.Prognostic value and clinicopathological significance of epithelial cadherin expression in non-small cell lung cancer[J].Thoracic Cancer,2015,6(5):589-596.
[7]Li LI,Lv Y,Zhang Y,et al.Expression and clinical significance of Oct-4 and E-cad in non-small-cell lung cancer[J].Oncol Lett,2016,11(1):234-236.
[8]Jiang W,Fan H,Qian C,et al.Prognostic value of high Fox C2 expression in resectable non-small cell lung cancer,alone or in combination with E-cadherin expression[J].BMC Cancer,2016,16(1):16.
[9]Thirkill L,Gao T,Stout M,et al.MUC1 is involved introphoblast transendothelial migration[J].Biochim Biophys Acta,2007,1773(6):1007-1014.
[10]Wei X,Lai Y,Li J,et al.PSCA and MUC1 in non-small-cell lung cancer as targets of chimeric antigen receptor T cells[J].Oncoimmunology,2017,6(3):e1284722.
[11]Teramoto K,Ozaki Y,Hanaoka J,et al.Predictive biomarkers and effectiveness of MUC1-targeted dendritic-cell-based vaccine in patients with refractory non-small cell lung cancer[J].Therapeutic Adv Med Oncol,2017,9(3):147-157.
[12]Ramlogan-Steel CA,Steel JC,Morris JC.Lung cancer vaccines:current status and future prospects[J].Transl Lung Cancer Res,2014,3(1):46-52.
[13]Obermair A,Schmid BC,Packer LM,et al.Expression of MUC1splice variants in benign and malignant ovarian tumors[J].International J Cancer,2002,100(2):166-171.
[14]Sritama Nath,Pinku Mukherjee.MUC1:a multifaceted oncoprotein with a key role in cancer progression[J].Trends Mol Med,2014,20(6):332-342.
[15]Kuemmel A,Single K,Bittinger F,et al.TA-MUC1 epitope in non-small cell lung cancer[J].Lung Cancer,2009,63(1)98-105.
[16]Wang-Rodriguez J,Dreilinger AD,Alsharabi GM,et al.The signaling adapter protein PINCH is up-regulated in the stroma of common cancer,notably at invasive edges[J].Cancer,2002,95(6):1387-1395.
[17]Zhang JT,Li QX,Wang D,et al.Up-regulation of PINCH in the stroma of oral squamous cell carcinoma predicts nodal metastasis[J].Oncol Rep,2005,14(6):1519-1522.
[18]魏路军任新友,徐林浩,等.E-cadherin、MUC1及PTEN与肿瘤转移相关性[J].青岛大学医学院学报,2010,46(2):183-185.Wei LJ,Ren XY,Xu LH,et al.Correlation of E-cadherin,MUC1and PTEN with tumor metastasis[J].Acta Acad Med Qingdao Universitatis,2010,46(2):183-185.[收稿]