广东客家茶树种质资源儿茶素特性分析
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摘要
为了更好地利用和开发广东客家茶树种质资源,以广东省客家茶树种质资源为研究对象,采用高效液相色谱法对儿茶素组分进行分析,通过因子和聚类分析法揭示了客家茶树种质资源的儿茶素特性,结果表明:客家茶树种质资源中EGCG、EGC和GCG为高含量组分,其中,EGCG的含量最高,EGCG在群体间和季节间变异系数最小。儿茶素总量和酯型儿茶素在群体间和季节间变异系数小于非酯型儿茶素。客家茶树种质资源中EGCG、酯型儿茶素和EGCG/酯型儿茶素比值低于大叶种茶树种质。因子和聚类分析显示,EGCG、ECG和EGC可作为不同茶树种质儿茶素的表征性成分;客家茶树种质资源区别于大叶种茶树资源,可单独聚为一类。
In order to preferably use and exploit the hakka tea germplasm resources in Guangdong,we analyzed the catechin components in these resources through high performance liquid chromatography,and revealed their catechin characteristics by using the methods of factor analysis and clustering analysis. The results indicated that: in these hakka tea germplasm resources,the contents of EGCG( epigallocatechin gallate),EGC( epigallocatechin) and GCG( gallocatechin gallate) were higher,especially the EGCG content was the highest,and the EGCG content had the smallest variability among different populations or seasons. The catechin total content and ester catechin content had smaller variability among different populations or seasons than non-ester catechin content. The EGCG content,ester catechin content,and ratio of EGCG content to ester catechin content in hakka tea germplasm resources were lower than those in large-leaf tea resources. The factor analysis and clustering analysis showed that: EGCG,ECG and EGC could be used as the representative components of catechins in various tea germplasms; the hakka tea germplasm resources could be alone clustered into a category,because they were different from large-leaf tea resources.
In order to preferably use and exploit the hakka tea germplasm resources in Guangdong,we analyzed the catechin components in these resources through high performance liquid chromatography,and revealed their catechin characteristics by using the methods of factor analysis and clustering analysis. The results indicated that: in these hakka tea germplasm resources,the contents of EGCG( epigallocatechin gallate),EGC( epigallocatechin) and GCG( gallocatechin gallate) were higher,especially the EGCG content was the highest,and the EGCG content had the smallest variability among different populations or seasons. The catechin total content and ester catechin content had smaller variability among different populations or seasons than non-ester catechin content. The EGCG content,ester catechin content,and ratio of EGCG content to ester catechin content in hakka tea germplasm resources were lower than those in large-leaf tea resources. The factor analysis and clustering analysis showed that: EGCG,ECG and EGC could be used as the representative components of catechins in various tea germplasms; the hakka tea germplasm resources could be alone clustered into a category,because they were different from large-leaf tea resources.
引文
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[12]张凯,丁阳平,杨坚.川渝地区野生大茶树儿茶素和咖啡碱含量比较分析[J].应用与环境生物学报,2013,19(2):379-382.
[13]金孝芳,贾尚智,石亚亚,等.湖北地方茶树种质资源中酯型儿茶素含量研究[J].热带作物学报,2014,35(3):438-442.
[2]卓敏,陈栋,王秋霜,等.广东客家茶的感官品质特征及其影响因子研究[J].广东农业科学,2011,38(10):79-81.
[3]王秋霜,刘淑媚,凌彩金.广东东源客家炒青绿茶矿物及重金属元素的ICP-MS测定[J].广东农业科学,2016,43(5):124-128.
[4] Wang H,Bian S J,Yang C S. Green tea polyphenol EGCG suppresses lung cancer cell growth through upregulating miR-210 expression caused by stabilizing HIF-1alpha[J]. Carcinogenesis,2011,32(12):1881-1189.
[5]Lee H,Bae J H,Lee S R. Protective effect of green tea polyphenol EGCG against neuronal damage and brain edema after unilateral cerebral ischemia in gerbils[J].Journal of Neuroscience Research,2004,77(6):892-900.
[6]Kallithraka S,Bakker J,Clifford M N. Evaluation of bitterness and astringency of(+)-catechin and(+)-epicatechin in red wine and in model solutions[J]. Journal of Sensory Studies,1997,12(1):25-37.
[7]陆锦时.茶树儿茶素含量及组成特性与品种品质的关系[J].西南农业学报,1994(s1):6-12.
[8]乔小燕,吴华玲,韩雪文,等.仁化白毛茶生化成分与成品白茶品质的相关性研究[J].核农学报,2015,29(12):2327-2333.
[9]王庆,罗军武,张征,等.青山野生茶树资源生物碱及儿茶素组分研究[J].湖南农业科学,2013(5):14-16.
[10]刘声传,段学艺,赵华富,等.贵州野生茶树种质资源生化多样性分析[J].植物遗传资源学报,2014,15(6):1255-1261.
[11]金孝芳,贾尚智,石亚亚,等.湖北省地方审定茶树品种中儿茶素含量分析[J].浙江农业学报,2014,26(1):67-71.
[12]张凯,丁阳平,杨坚.川渝地区野生大茶树儿茶素和咖啡碱含量比较分析[J].应用与环境生物学报,2013,19(2):379-382.
[13]金孝芳,贾尚智,石亚亚,等.湖北地方茶树种质资源中酯型儿茶素含量研究[J].热带作物学报,2014,35(3):438-442.