番茄红素在不同溶剂中的分光光度法分析
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摘要
番茄红素因其抗氧化活性强,具有多种养生保健功效,日益受到重视,但番茄红素的提取测定方法繁琐复杂,很难广泛应用。采用分光光度法,以石油醚、丙酮、甲醇为溶剂,二氯甲烷为助溶剂,先从吸收光谱差异入手,确定最大吸收波长,再于最大吸收波长处,以不同浓度的番茄红素标准液测定其吸光度值,制作标准曲线,得到回归方程,并考察二氯甲烷的量,浸提时间等对番茄红素提取效果的影响,探索分光光度法测定番茄红素的简单快速的分析方法。实验结果表明,以石油醚为溶剂,波长474 nm,加入5 mL的二氯甲烷助溶,浸提时间为40 min时,是用分光光度法测定番茄红素的最佳方法。
In recent years, lycopene has been paid more and more attention due to its strong antioxidant activity and various health care functions. However, lycopene extraction and determination methods are complicated and difficult to be widely applied. In this study, using spectrophotometry, petroleum ether, acetone and methanol as solvent, methylene chloride as co-solvent, starting with the absorption spectrum differences to determine the maximum absorption wavelength, then, the absorbance of lycopene standard liquid with different solubility was determined at the maximum absorption wavelength and the standard curve was established to obtain the regression equation. The effect of the amount of dichloromethane and the extraction time on the lycopene extraction efficiency was investigated, and the simple and rapid determination method of lycopene by spectrophotometry was explored. The experimental results showed that the best method for the determination of lycopene by spectrophotometry is petroleum ether as solvent, wavelength 474 nm, addition of 5 mL of dichloromethane for solubilization and extraction time of 40 min.
In recent years, lycopene has been paid more and more attention due to its strong antioxidant activity and various health care functions. However, lycopene extraction and determination methods are complicated and difficult to be widely applied. In this study, using spectrophotometry, petroleum ether, acetone and methanol as solvent, methylene chloride as co-solvent, starting with the absorption spectrum differences to determine the maximum absorption wavelength, then, the absorbance of lycopene standard liquid with different solubility was determined at the maximum absorption wavelength and the standard curve was established to obtain the regression equation. The effect of the amount of dichloromethane and the extraction time on the lycopene extraction efficiency was investigated, and the simple and rapid determination method of lycopene by spectrophotometry was explored. The experimental results showed that the best method for the determination of lycopene by spectrophotometry is petroleum ether as solvent, wavelength 474 nm, addition of 5 mL of dichloromethane for solubilization and extraction time of 40 min.
引文
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[2] SONG Li, ZHAI Qiu-ge, FU Yu, et al(宋力, 翟秋阁, 付裕, 等). Journal of Xinyang Normal University(信阳师范学院学报), 2012, 25(1): 109.
[3] ZHONG Shi-li(钟世丽). Sino-Foreign Food Industry(中外食品工业), 2014,(12): 9.
[4] LIU Li-guo, WU Jing(刘立国, 吴晶). Science and Technology of Food Industry(食品工业科技), 2002, 23(4): 74.
[5] SUI Hai-xia, XU Hai-bin, YAN Wei-xing(隋海霞, 徐海滨, 严卫星). Chinese Journal of Food Hygiene(中国食品卫生杂志), 2003, 15(6): 544.
[6] WEI Lai, ZHAO Chun-jing(魏来, 赵春景). China Pharmaceuticals(中国药业), 2004, 13(10): 21.
[7] SU Yong-heng, JIANG Hui-ran, LI Fa-sheng(苏永恒, 蒋惠然, 李发生). Chinese Journal of Health Laboratory Technology(中国卫生检验杂志), 2004, 14(6): 708.
[8] ZHENG Li-li, CHEN Wen, XIE Qiao, et al(郑丽丽, 陈文, 谢巧, 等). Journal of Shihezi University·Natural Science(石河子大学学报·自然科学版), 2006, 24(5): 579.
[9] XIA Ping, SHENG Jian-jun(夏萍, 盛建军). Applied Chemistry Industry(应用化工), 2007, 36(10): 975.