正交法优选双乌镇痛胶囊醇提取工艺
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摘要
目的:研究溶剂倍数、乙醇浓度、回流时间、回流次数等因素对乙醇回流提取的影响,优选双乌镇痛胶囊中制川乌、制草乌、青风藤、独活的提取工艺。方法:采用正交试验法,以干膏得率和青藤碱含量为评价指标,分析乙醇溶度、回流时间、回流次数、溶剂倍数4个提取因素。结果:确定双乌镇痛胶囊的最佳提取工艺为制川乌、制草乌、青风藤、独活加85%乙醇回流提取3次,第一次加5倍量溶剂回流1. 5 h;第二次加4倍量溶剂回流1. 5 h;第三次加4倍量溶剂回流1. 5 h。结论:双乌镇痛胶囊的提取工艺简单,提取效率稳定,试验结果准确。
Objective: To optimize the extraction process of Zhichuanwu(Radix Aconiti),Zhicaowu(Radix Aconiti Majus),Qingfengteng(Radix Sinensis) and Duhuo(Radix Angelicae Solanum) in Shuangwu Zhentong Capsule,four factors were studied. Methods: With the yield of dry paste and sinomenine content as the evaluation indexes,the four extraction factors including ethanol solubility,reflux time,reflux times and solvent multiple were analyzed by orthogonal test. Results: The optimum extraction conditions of Shuangwu Zhentong Capsules were determined as follows: Zhichuanwu(Radix Aconiti),Zhicaowu(Radix Aconiti Majus),Qingfengteng(Radix Sinensis) and Duhuo(Radix Angelicae Solanum) with 85% ethanol were refluxed and extracted for 3 times,with5 times' solvent refluxing for 1. 5 hours for the first time,4 times' solvent refluxing 1. 5 hours for the second time,and 4 times' solvent refluxing for 1. 5 hours for the third time. Conclusion: The extraction technology of Shuangwu Zhentong Capsule is simple,the extraction efficiency is stable,and the result is accurate.
Objective: To optimize the extraction process of Zhichuanwu(Radix Aconiti),Zhicaowu(Radix Aconiti Majus),Qingfengteng(Radix Sinensis) and Duhuo(Radix Angelicae Solanum) in Shuangwu Zhentong Capsule,four factors were studied. Methods: With the yield of dry paste and sinomenine content as the evaluation indexes,the four extraction factors including ethanol solubility,reflux time,reflux times and solvent multiple were analyzed by orthogonal test. Results: The optimum extraction conditions of Shuangwu Zhentong Capsules were determined as follows: Zhichuanwu(Radix Aconiti),Zhicaowu(Radix Aconiti Majus),Qingfengteng(Radix Sinensis) and Duhuo(Radix Angelicae Solanum) with 85% ethanol were refluxed and extracted for 3 times,with5 times' solvent refluxing for 1. 5 hours for the first time,4 times' solvent refluxing 1. 5 hours for the second time,and 4 times' solvent refluxing for 1. 5 hours for the third time. Conclusion: The extraction technology of Shuangwu Zhentong Capsule is simple,the extraction efficiency is stable,and the result is accurate.
引文
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[15]郝少君,王希东,马珍珍,等,双乌镇痛胶囊对大鼠血液生化指标的影响[J].中华中医药学刊,2015,33(11):2746-2747.
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[3]沙世炎,徐礼桑.中草药有效成分分析[M].北京:人民卫生出版社,1992:52.
[4]谢秀琼.中药新制剂开发与应用[M].北京:人民卫生出版社,1994:61.
[5]王北婴.中药新药研制与申报[M].北京:中国中医药出版社,1995:21.
[6]国家药品监督管理局.中药新药研究的技术要求[M].北京:人民卫生出版社,1999:15.
[7]孙文基.天然药物成分定量分析[M].北京:中国医药科技出版社,2003:19.
[8]尹明晓,张金杰,翁远超,等.正交设计优化枳实中3种二氢黄酮苷的提取工艺[J].实用药物与临床,2013,16(2):127-129.
[9]李重先,李文俊,郝少君,等.双乌镇痛胶囊室温留样稳定性研究[J].中国医院用药评价与分析,2016,16(1):92-93.
[10]郝少君,王希东,李文俊,等.抗眩颗粒对小鼠自主活动及运动协调能力的影响[J].中医学报,2014,29(2):237-238.
[11]郝少君,王灵,王希东,等.双乌镇痛胶囊对大鼠食量和体质量的影响[J].中医学报,2014,29(12):1776-1777.
[12]郝少君,王希东,张正臣.双乌镇痛胶囊对小鼠痛阈和压痛阈的影响[J].河北中医,2014,36(8):1226-1228.
[13]郝少君,王希东,张正臣.双乌镇痛胶囊恒温、恒湿加速试验稳定性研究[J].河北医药,2015,37(2):288-289.
[14]吕宏迪,郝少君,温中华.双乌镇痛胶囊对大鼠脏器系数的影响[J].河北中医,2015,36(1):67-69.
[15]郝少君,王希东,马珍珍,等,双乌镇痛胶囊对大鼠血液生化指标的影响[J].中华中医药学刊,2015,33(11):2746-2747.