大黄素、茶多酚和鱼腥草素钠对大鼠结肠炎的治疗作用
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摘要
目的观察大黄素、茶多酚和鱼腥草素钠对2,4,6-三硝基苯磺酸(TNBS)诱导大鼠结肠炎的治疗作用,并探讨其可能的免疫机制。方法将90只SD大鼠随机分为9组,采用TNBS诱导建立炎症性肠病大鼠模型。造模24 h后,定时ig给予药物治疗组大鼠相应剂量的药物,空白对照组和模型组大鼠ig给予同体积的蒸馏水,每天1次,持续14 d。观察大鼠每日的体重变化、大便状态,解剖大鼠后观察结肠组织的大体改变和病理变化,检测大鼠血清中肿瘤坏死因子α、白介素-lβ(IL-lβ)、IL-4、IL-10的水平及丙氨酸氨基转移酶、天门冬氨酸氨基转移酶、肌酐的含量。结果与空白对照组比较,药物治疗组和模型组大鼠的一般情况较差,体重明显减轻,炎性指标显著减低;与模型组比较,除鱼腥草素钠低剂量组外,其余药物治疗组大鼠的体重均明显增加,炎症情况明显缓解,炎性指标显著降低;茶多酚和鱼腥草素钠高剂量组改善炎症的效果显著优于低剂量组的,而鱼腥草素钠低剂量组的抗炎效果与模型组的比较无显著性差异。结论大黄素、茶多酚和鱼腥草素钠均对大鼠结肠炎有治疗作用,其中,茶多酚和鱼腥草素钠的治疗作用存在量效关系。
OBJECTIVE To observe the therapeutic effects of emodin,tea polyphenols and sodium houtuyfonate oninflammatory bowel disease in rats induced by TNBS,and to explore the possible immune mechanism. METHODS NinetySprague Dawley rats were randomly divided into nine groups,respectively. Colitis model was induced by TNBS. Molding after 24 hours,the therapy groups were given drug once a day with a given dose for 14 days,the model group and control group were givendistilled water of the same volume daily. The body weights and stool state of rats were recorded every day. The general change andhistologic pathological change after HE staining were observed. The level of TNF-α,IL-lβ,IL-4 and IL-10 and the contentof ALT,AST were detected,as well as creatinine in serum. RESULTS Compared with the control group,the therapy groups andmodel group had poor general situation,decreasing weight,and the inflammatory indicators reduced significantly. Compared withthe model group,the therapy groups had better general situation,significantly reduced intestinal inflammation,and theinflammatory indicators reduced significantly. The high dose groups of tea polyphenols and sodium houtuyfonate had better anti-inflammatory effect than the low dose groups. There was no statistically significant difference between the sodium houtuyfonate lowdose group and the model group. CONCLUSION Emodin,tea polyphenols and sodium houtuyfonate all have the therapeuticeffect on rats colitis. There is a dose-dependent relationship on tea polyphenols and sodium houtuyfonate because of thetherapeutic effect on rats colitis.
OBJECTIVE To observe the therapeutic effects of emodin,tea polyphenols and sodium houtuyfonate oninflammatory bowel disease in rats induced by TNBS,and to explore the possible immune mechanism. METHODS NinetySprague Dawley rats were randomly divided into nine groups,respectively. Colitis model was induced by TNBS. Molding after 24 hours,the therapy groups were given drug once a day with a given dose for 14 days,the model group and control group were givendistilled water of the same volume daily. The body weights and stool state of rats were recorded every day. The general change andhistologic pathological change after HE staining were observed. The level of TNF-α,IL-lβ,IL-4 and IL-10 and the contentof ALT,AST were detected,as well as creatinine in serum. RESULTS Compared with the control group,the therapy groups andmodel group had poor general situation,decreasing weight,and the inflammatory indicators reduced significantly. Compared withthe model group,the therapy groups had better general situation,significantly reduced intestinal inflammation,and theinflammatory indicators reduced significantly. The high dose groups of tea polyphenols and sodium houtuyfonate had better anti-inflammatory effect than the low dose groups. There was no statistically significant difference between the sodium houtuyfonate lowdose group and the model group. CONCLUSION Emodin,tea polyphenols and sodium houtuyfonate all have the therapeuticeffect on rats colitis. There is a dose-dependent relationship on tea polyphenols and sodium houtuyfonate because of thetherapeutic effect on rats colitis.
引文
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[8]Guidi L,Costanzo M,Ciarniello M,et al.Increased levels of NF-kappa B inhibitors(IkappaBalpha and IkappaBgamma)in the intestinal mucosa of Crohn’s disease patients during infliximab treatment[J].Int J Immunopathol Pharmacol,2005,18(1):155-164.
[9]宋莎莎,杨乐,俞令凯,等.合成鱼腥草素对溃疡性结肠炎小鼠的保护作用[J].长江大学学报,2014,11(16):67-69.
[10]牛华英,赵桂森.鱼腥草素类药物的研究[D].济南:山东大学硕士学位论文,2006.
[2]李灵玲,蒲吉.大黄素促进结肠癌细胞自噬性凋亡的作用及其机制[J].华西药学杂志,2017,32(6):603-606.
[3]李清钊,刘桂香,姚林.顺铂对机体损伤及茶多酚的拮抗作用[J].华西药学杂志,2001,16(1):36-37.
[4]CherbutC,Michel C,Lecannu G.T he prebiotic characteristics of fructooligosac-charides are necessary for reduction of TNBS-induced colitis in rats[J].Nutr,2003,133:21-27.
[5]Liu Z,Yadav PK,Xu X,et al.The increased expression of IL-23 in inflammatory bowel disease promotes intraepithelial and lamina propria lymphocyte inflammatory responses and cytotoxicity[J].Leukoc Biol,2011,89:597-606.
[6]Mowat C,Cole A,Windsor A,et al.Guidelines for the management of inflammat bowel disease in adults[J].Gut,2011,60(5):571-607.
[7]de Lima A,Zelinkova Z,van der Ent C,et al.Tailored antiTNF therapy during pregnancy in patients with IBD:Maternal and fetal safety[J].Gut,2016,65:1261-1268.
[8]Guidi L,Costanzo M,Ciarniello M,et al.Increased levels of NF-kappa B inhibitors(IkappaBalpha and IkappaBgamma)in the intestinal mucosa of Crohn’s disease patients during infliximab treatment[J].Int J Immunopathol Pharmacol,2005,18(1):155-164.
[9]宋莎莎,杨乐,俞令凯,等.合成鱼腥草素对溃疡性结肠炎小鼠的保护作用[J].长江大学学报,2014,11(16):67-69.
[10]牛华英,赵桂森.鱼腥草素类药物的研究[D].济南:山东大学硕士学位论文,2006.