不同产地郁金茎叶挥发油化学成分及指纹图谱对比研究
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摘要
目的:研究不同产地郁金茎叶的GC-MS指纹图谱,以期为郁金茎叶的质量标准建立提供依据。方法:通过水蒸气蒸馏法提取10个不同产地郁金茎叶中挥发油成分,运用GC-MS分析10个产地郁金茎叶挥发油成分的组成与含量差异,建立指纹图谱,所得图谱采用"中药色谱指纹图谱相似度评价系统A版"软件进行分析并计算相似度。结果:10个批次郁金茎叶挥发油指纹图谱有11个共有峰,其中参照峰为桉叶油醇。除了四川产黄丝郁金、广西产桂郁金、乐清虹桥2号、瑞安湖岭产温郁金茎叶指纹图谱相似度<0.9,浙江瑞安马屿、乐清白石、瑞安陶山、永嘉乌牛、乐清虹桥1号、丽水云和6个产地温郁金茎叶指纹图谱相似度均>0.9。结论:不同品种的郁金茎叶挥发油成分差异较大,不同产地的温郁金茎叶挥发油成分指纹图谱也存在差异。
Objective: In order to provide the basis for the establishment of quality standard of the stem and leave of Curcumae Radix,this paper researched GC-MS fingerprint of the stems and leaves of Curcumae Radix from different origins. Methods: Extract the constituents of volatile oil from 10 different origins in the stems and leaves of Curcumae Radix by steam distillation extraction method. Analyze the differences in composition and content of the 10 different origins of the stems and leaves of Curcumae Radix by GC-MS and calculate the similarity of the atlas by Chromatographic Fingerprint Similarity Evaluation System A Edition software. Results: The fingerprints of the stem and leave of Curcumae Radix from the 10 groups had 11 common peaks with reference to the peak of eucalyptus. The similarity of the fingerprints of Rui'an Mayu,Yueqing Baishi,Rui'an Taoshan,Yongjia Wuniu,Yueqing Hongqiao No. 1,and Lishui Yunhe was above0. 90. The similarity of the fingerprint of Sichuan,Guangxi,Yueqing Hongqiao No. 2 and Rui'an Huling was less than0. 90. Conclusion: Different varieties of Curcumae Radix have much difference in the composition of volatile oil of the stems and leaves and there is also a difference in the fingerprints of composition of volatile oil of the stems and leaves of Curcuma wenyujin Y. H. Chen et C. Ling from different origins.
Objective: In order to provide the basis for the establishment of quality standard of the stem and leave of Curcumae Radix,this paper researched GC-MS fingerprint of the stems and leaves of Curcumae Radix from different origins. Methods: Extract the constituents of volatile oil from 10 different origins in the stems and leaves of Curcumae Radix by steam distillation extraction method. Analyze the differences in composition and content of the 10 different origins of the stems and leaves of Curcumae Radix by GC-MS and calculate the similarity of the atlas by Chromatographic Fingerprint Similarity Evaluation System A Edition software. Results: The fingerprints of the stem and leave of Curcumae Radix from the 10 groups had 11 common peaks with reference to the peak of eucalyptus. The similarity of the fingerprints of Rui'an Mayu,Yueqing Baishi,Rui'an Taoshan,Yongjia Wuniu,Yueqing Hongqiao No. 1,and Lishui Yunhe was above0. 90. The similarity of the fingerprint of Sichuan,Guangxi,Yueqing Hongqiao No. 2 and Rui'an Huling was less than0. 90. Conclusion: Different varieties of Curcumae Radix have much difference in the composition of volatile oil of the stems and leaves and there is also a difference in the fingerprints of composition of volatile oil of the stems and leaves of Curcuma wenyujin Y. H. Chen et C. Ling from different origins.
引文
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[11]孙国祥,闫波,侯志飞,等.中药色谱指纹图谱评价方法研究进展[J].中南药学,2015,13(7):673-681.
[12]刘玉梅.指纹图谱在中药质量控制中的应用简析[J].亚太传统医药,2015,11(13):56-57.
[13]Yanwei Wang,Wenying Gao,Isao Noda,et al.A modified mean normalization method to reduce the effect of peak overlap in two-dimensional correlation spectroscopy[J].Journal of Molecular Structure,2006,799(1-3):128-133.
[14]刘秀明,王家俊,李源栋,等.GC/MS指纹图谱结合Hotelling T2检验用于香精品质控制[J].烟草科技,2015,48(7):36-40.
[15]杨生超,赵昶灵,文国松,等.植物药材道地性的分子机制研究与应用[J].中草药,2007,38(11):1738-1741.
[2]方露敏,黄真.温郁金的研究进展[J].中华中医药学刊,2008,26(9):1998-2000.
[3]汤淙淙,秦坤良,黄可新.温郁金茎叶化学成分及抗肿瘤活性[J].温州医学院学报,2007,37(2):110-113.
[4]项秀娣,陈素红,楼招欢.气相色谱法测定不同方法提取的莪术挥发油中β-榄香烯的含量[J].浙江中医药大学学报,2011,35(3):397-398,409.
[5]马骁驰,邓卅,王长远,等.桔梗悬浮细胞对莪二酮的生物转化研究[J].现代生物医学进展,2010,10(18):3537-3540.
[6]秦坤良,汤淙淙,黄可新,等.温郁金茎叶与块根中挥发油成分的比较研究[J].温州医学院学报,2006,36(2):95-97.
[7]国家药典委员会.中华人民共和国药典[S].北京:中国医药科技出版社,2010:附录63.
[8]陈丛瑾,黄克瀛,黄玉松,等.鲜姜黄挥发油化学成分的GCMS分析[J].中华中医药杂志,2009,3(24):364-366.
[9]张春椿,年慧慧,李效贤,等.GC/MS法对南方红豆杉种皮抑制物分析研究[J].浙江中医药大学学报,2013,37(4):446-450.
[10]Ju-feng Zhou,Xuan-qi Fang,Tai-ming Zhang,et al.Quantitative similarity assessment of non-linear chemical fingerprint of traditional Chinese medicine by similarity system theory[J].Journal of Central South University of Technology,2011,18(2):343-352.
[11]孙国祥,闫波,侯志飞,等.中药色谱指纹图谱评价方法研究进展[J].中南药学,2015,13(7):673-681.
[12]刘玉梅.指纹图谱在中药质量控制中的应用简析[J].亚太传统医药,2015,11(13):56-57.
[13]Yanwei Wang,Wenying Gao,Isao Noda,et al.A modified mean normalization method to reduce the effect of peak overlap in two-dimensional correlation spectroscopy[J].Journal of Molecular Structure,2006,799(1-3):128-133.
[14]刘秀明,王家俊,李源栋,等.GC/MS指纹图谱结合Hotelling T2检验用于香精品质控制[J].烟草科技,2015,48(7):36-40.
[15]杨生超,赵昶灵,文国松,等.植物药材道地性的分子机制研究与应用[J].中草药,2007,38(11):1738-1741.