植物生长调节剂对木薯组织培养再生的影响
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摘要
以木薯(Manihot esculenta Crantz)华南102无菌苗为试验材料,研究植物生长调节剂对木薯组织培养再生的影响。结果表明,当MS培养基中添加0.4 mg/L KT时木薯腋芽增殖效果最好;1/2 MS培养基中添加MET能使木薯组培苗根变粗壮,添加浓度以0.4 mg/L为宜;单独添加2,4-D可诱导木薯无菌苗叶片愈伤组织,同时添加6-BA可改善愈伤组织质地。
The effects of plant growth regulators on in vitro plant regeneration of cassava were investigated through tissue culture methods using seedlings derived from sterile germination of cassava varieties of huanan 102 as material. Experiment results showed that the proliferation of cassava axillary bud turned out best while added the 0.4 mg/L KT into the MS culture medium. The roots of cassava tissue cultured seedlings grew stout while added the 0.4 mg/L MET into the 1/2 MS Medium.2,4-D could induced callus of the leaves of cassava without vaccine,Meanwhile added 6-BA could improved the texture of callus.
The effects of plant growth regulators on in vitro plant regeneration of cassava were investigated through tissue culture methods using seedlings derived from sterile germination of cassava varieties of huanan 102 as material. Experiment results showed that the proliferation of cassava axillary bud turned out best while added the 0.4 mg/L KT into the MS culture medium. The roots of cassava tissue cultured seedlings grew stout while added the 0.4 mg/L MET into the 1/2 MS Medium.2,4-D could induced callus of the leaves of cassava without vaccine,Meanwhile added 6-BA could improved the texture of callus.
引文
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[2]张慧坚,刘恩平,刘海清,等.广西木薯产业发展状况与对策[J].广东农业科学,2012,5(6):161-164.
[3]何晶.广西木薯产业发展研究[D].南宁:广西大学,2012.
[4]黄强,李军.广西木薯种质资源的收集利用和品种选育[J].广西热带农业,2007(1):35-37.
[5]文峰,苏文潘,黄建祺,等.木薯再生体系建立的研究进展[J].安徽农业科学,2017,45(8):156-158.
[6]曾文丹,陆柳英,谢向誉,等.优良木薯品种新选048离体快繁技术[J].南方农业学报,2014,45(6):950-954.
[7]周珊.紫心马铃薯“黑美人”品种的组织培养[D].南宁:广西大学,2012.
[8]李琰,张朝红,崔宏安,等.杜仲愈伤组织诱导的研究[J].西北农林科技大学学报(自然科学版),2003,31(5):153-157.
[9]龚玉莲,陈坚毅,曾碧健.多效唑在植物组织培养中应用前景的探讨[J].广东教育学院学报,2000,20(3):103-106.
[10]潘瑞炽.植物组织培养[M].第七版.北京:高等教育出版社,2012.