沙生蜡菊总黄酮最佳提取工艺的研究
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摘要
目的建立沙生蜡菊总黄酮最佳提取工艺。方法采用UV法进行总黄酮的含量测定,以槲皮素为对照品,以乙醇浓度、料液比、提取时间为提取工艺的考察因素,沙生蜡菊总黄酮含量作为提取工艺的评价指标,采用正交实验对沙生蜡菊总黄酮提取工艺进行优化。结果沙生蜡菊总黄酮最佳提取工艺为乙醇浓度60%,料液比1∶20,提取时间120 min,沙生蜡菊提取物中总黄酮的平均含量为46.572 mg/g。结论本提取工艺方法简单、高效,适用于沙生蜡菊总黄酮的提取。
Objective To establish the optimum extraction technology of total flavones. Methods The content of total flavonoids was determined by UV with quercetin being used as standard control. Ethanol concentration, material-liquid ratio and extraction time were taken as the factors to investigate the extraction process, and the content of total flavonoids of Helichrysum arenarium(L) Moench. Was taken as the index of extraction process, orthogonal experiment was used to optimize the extraction technology of total flavonoids extracted from Helichrysum arenarium(L) Moench. Results The optimum extraction process was 60% ethanol, 1∶20 ratio of material to liquid, 120 min. The average content of total flavonoids was 46.572 mg/g. Conclusion The extraction process we reported here was simple and efficient, and is suitable for the extraction, separation and purification of total flavonoids from Helichrysum arenarium(L) Moench.
Objective To establish the optimum extraction technology of total flavones. Methods The content of total flavonoids was determined by UV with quercetin being used as standard control. Ethanol concentration, material-liquid ratio and extraction time were taken as the factors to investigate the extraction process, and the content of total flavonoids of Helichrysum arenarium(L) Moench. Was taken as the index of extraction process, orthogonal experiment was used to optimize the extraction technology of total flavonoids extracted from Helichrysum arenarium(L) Moench. Results The optimum extraction process was 60% ethanol, 1∶20 ratio of material to liquid, 120 min. The average content of total flavonoids was 46.572 mg/g. Conclusion The extraction process we reported here was simple and efficient, and is suitable for the extraction, separation and purification of total flavonoids from Helichrysum arenarium(L) Moench.
引文
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[3] 刘禾培,窦君,马纪,等.三种维药提取物对蛋白酪氨酸磷酸酶1B抑制作用的研究[J].天然产物研究与开发,2011,23(1):142-145.
[4] 陈彩云,尹淑英,林莺,等.黄豆中总黄酮提取及不同豆类抗氧化活性研究[J].辽宁中医药大学学报,2019,21(4):70-73.
[5] 胡煌.紫苏叶黄酮提取工艺优化及其抗氧化活性研究[J].发酵科技通讯,2019,48 (1):23-28.
[6] 高建德,宋开蓉,朱晓玉,等.复方苁蓉颗粒总黄酮提取工艺优化及其体外抗氧化活性[J].中成药,2018,40(7):1496-1500.
[7] 魏星任,宋晓娟,龙洁怡,等.仙草总黄酮提取纯化工艺优化及其抗氧化活性[J].中成药,2018,40(5):1185-1188.
[8] 龙旭,高静,张光辉,等.竹叶黄酮的提取、纯化及抗氧化性能研究[J].当代化工,2019,48(2):299-302.
[9] 王立波,王健伟,甘春丽,等.沙生蜡菊花降脂活性部位的化学成分[J].沈阳药科大学学报,2011,28(11):868-870,916.
[10] 王立波,刘凤芝,甘春丽,等.沙生蜡菊花降脂活性部位化学成分的分离与鉴定(Ⅱ)[J].沈阳药科大学学报,2012,29(2):109-112,125.
[11] 王立波,王健伟,王策,等.沙生蜡菊花降脂活性部位的化学成(Ⅲ)[J].中国药物化学杂志,2012,22(3):220-222,226.
[12] 巩新玉,闫晗,翟萌萌,等.沙生蜡菊的化学成分研究[J].亚太传统医药,2012,8(6):36-38.
[13] 刘文娟,吴立军.沙生蜡菊花中的黄酮类成分[J].中国中药杂志,2009,34(11):1381-1383.
[14] 刘海洋,何红平,杨献文,等.蜡菊花的化学成分研究[J].天然产物研究与开发,2007,16(3):423-426.
[15] 王立波,森川敏生,高慧媛,等.沙生蜡菊花中的二氢黄酮类成分[J].中国天然药物,2009,7(5):357-360.
[16] 王立波,森川敏生,高慧媛,等.沙生蜡菊花中黄酮类成分的分离与鉴定[J].沈阳药科大学学报,2009,26(10):792-795.