安徽省适生马尾松优良种源子代抗松材线虫病评价及生长性状研究
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摘要
从安徽省马尾松种源加密实生种子园选择出生长较好的H、I、J、K、S、M、N等7个种源63个家系的子代,进行了抗松材线虫病性测定及生长性状研究。结果表明:在第1次人工接种松材线虫后,湖南省安化县(N)种源子代的生存率最高(80.7%),而贵州省铜仁县(S)种源子代的生存率最低(27.7%),7个种源子代的平均存活率为38.3%;在第2次人工接种后,还是N种源子代的生存率最高(41.8%),而湖南省永顺县(M)种源子代的生存率最低(2.4%),7个种源子代的平均存活率为6.4%;对抗病性测定的偏差值进行分级,发现湖南安化种源(N)在安徽省的抗病性最强;7个参试种源无论是苗期生长表现还是13年后各生长指标都远远优于当地对照潜山种源。
The offspring of 63 lines from 7 Pinus massoniana provenances(H, I, J, K, S, M and N) which grew well in Anhui province were selected out, and their growth traits and resistance to pine wilt disease(Bursaphelenchus xylophilus) were studied. The results indicated that: after the first artificial inoculation with B. xylophilus, the offspring of P. massoniana provenance N from Anhua county, Hunan province had the highest survival rate(80.7%), while the offspring of P. massoniana provenance S from Tongren county, Guizhou province had the lowest survival rate(27.7%), and the average survival rate of offspring of 7 provenances was 38.3%; after the second artificial inoculation with B. xylophilus, the offspring of P. massoniana provenance N still had the highest survival rate(41.8%), while the offspring of P. massoniana provenance M from Yongshun county, Hunan province had the lowest survival rate(2.4%), and the average survival rate of offspring of 7 provenances was 6.4%. The deviation values of nematode-resistance determination were graded, and it was found that: P. massoniana provenance N had the strongest nematode-resistance in Anhui province; the tested 7 P. massoniana provenances had much better growth indexes both at seedling stage and at 13-year-old stage than the local control provenance Qianshan.
The offspring of 63 lines from 7 Pinus massoniana provenances(H, I, J, K, S, M and N) which grew well in Anhui province were selected out, and their growth traits and resistance to pine wilt disease(Bursaphelenchus xylophilus) were studied. The results indicated that: after the first artificial inoculation with B. xylophilus, the offspring of P. massoniana provenance N from Anhua county, Hunan province had the highest survival rate(80.7%), while the offspring of P. massoniana provenance S from Tongren county, Guizhou province had the lowest survival rate(27.7%), and the average survival rate of offspring of 7 provenances was 38.3%; after the second artificial inoculation with B. xylophilus, the offspring of P. massoniana provenance N still had the highest survival rate(41.8%), while the offspring of P. massoniana provenance M from Yongshun county, Hunan province had the lowest survival rate(2.4%), and the average survival rate of offspring of 7 provenances was 6.4%. The deviation values of nematode-resistance determination were graded, and it was found that: P. massoniana provenance N had the strongest nematode-resistance in Anhui province; the tested 7 P. massoniana provenances had much better growth indexes both at seedling stage and at 13-year-old stage than the local control provenance Qianshan.
引文
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[17] 章健,徐六一,高景斌,等.抗松材线虫病马尾松无性系种子园营建技术[J].林业科技开发,2012,26(1):82-85.
[18] 徐六一,户田忠雄.马尾松松材线虫抗性候选木的选拔及评价研究[J].安徽农业科学,2006,34(17):4303-4304.
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[20] 徐六一,高景斌,章健,等.马尾松抗松材线虫病无性系的抗病性评价方法研究[J].安徽农业大学学报,2011,38(6):848-853.
[21] 皖D/LY02—84.安徽省苗木标准[S].
[22] 周志春,傅玉狮,吴天林.马尾松生长和材性的地理遗传变异及最优种源区的划定[J].林业科学研究,1993,6(5):556-563.
[23] 陈晓阳,沈熙环.林木育种学[M].北京:高等教育出版社,2007.
[24] 刘青华,周志春,范辉华,等.马尾松产脂力与生长性状的家系变异及优良家系早期选择[J].林业科学研究,2013,26(6):686-691.
[2] 秦国锋,周志春,等.中国马尾松优良种质资源[M].北京:中国林业出版社,2012.
[3] 周志春,金国庆.马尾松不同产地的遗传稳定性和生态学基础[J].南京林业大学学报,1998,22(3):75-80.
[4] 秦国锋.马尾松改良及培育[M].杭州:浙江大学出版社,2000.
[5] 林道清.马尾松种源有限区试验[J].林业科技开发,2003,17(2):22-24.
[6] 戸田忠雄,藤本吉幸,西村慶二.ら.九州地区におけるマツノザイセンチュウ抵抗性個体の選定[J].林育研究,1989(7):145-178.
[7] Mota M M, Vieira P. Pine wilt disease: a worldwide threat to forest ecosystems [M]. New York: Springer, 2008.
[8] 王明旭,罗宽.人工接种后次代抗松材线虫松树的抗病性[J].湖南农业大学学报:自然科学版,2005,31(2):159-161.
[9] 傅军.安徽省松材线虫病防治的思考[J].安徽林业科技,2011,37(4):43-44.
[10] 杨宝君,潘宏阳,汤坚,等.松材线虫病[M].北京:中国林业出版社,2003.
[11] 张星耀,骆有庆.中国森林重大生物灾害[M].北京:中国林业出版社,2003:1-8.
[12] 张锴,梁军,严冬辉,等.中国松材线虫病研究[J].世界林业研究,2010,23(3):59-63.
[13] GB8822.6—1988.中国林木种子区:马尾松种子区[S].
[14] 张晓渡,肖正东,程金年,等.马尾松种源实生种子园主要性状遗传变异的研究[J].浙江林业科技,2004,24(4):5-8.
[15] 黄俊.马尾松种源加密实生种子园子代测定的初步研究[J].安徽林业科技,2011,37(4):7-10.
[16] 徐六一,章健,高景斌,等.安徽省松材线虫病抗性育种研究进展[J].安徽林业科技,2013,39(2):8-10.
[17] 章健,徐六一,高景斌,等.抗松材线虫病马尾松无性系种子园营建技术[J].林业科技开发,2012,26(1):82-85.
[18] 徐六一,户田忠雄.马尾松松材线虫抗性候选木的选拔及评价研究[J].安徽农业科学,2006,34(17):4303-4304.
[19] 高景斌,席启俊,孙主义,等.松材线虫病抗性马尾松苗木的选育[J].林业科技开发,2009,23(1):91-95.
[20] 徐六一,高景斌,章健,等.马尾松抗松材线虫病无性系的抗病性评价方法研究[J].安徽农业大学学报,2011,38(6):848-853.
[21] 皖D/LY02—84.安徽省苗木标准[S].
[22] 周志春,傅玉狮,吴天林.马尾松生长和材性的地理遗传变异及最优种源区的划定[J].林业科学研究,1993,6(5):556-563.
[23] 陈晓阳,沈熙环.林木育种学[M].北京:高等教育出版社,2007.
[24] 刘青华,周志春,范辉华,等.马尾松产脂力与生长性状的家系变异及优良家系早期选择[J].林业科学研究,2013,26(6):686-691.