辽宁省粳稻品种稻瘟病抗性基因分析
|
摘要
【目的】为明确辽宁省260份粳稻品种中稻瘟病抗性基因的类型,评价抗性基因利用价值,为辽宁省水稻抗病育种提供参考。【方法】选用9个稻瘟病抗性基因的特异引物分析辽宁省已审定的260份粳稻品种抗性基因种类、基因组合类型及地域分布情况,基于抗病基因检测条带的有无进行品种聚类分析,苗期人工喷雾接种、分蘖盛期和蜡熟期田间自然发病调查,评价9个抗性单基因系的抗病性。【结果】辽宁省粳稻品种中含有抗性基因数量从0～7个不等,抗性基因Pikh检出率最高,其次是Pikm和Pi1;所有参试品种均未检测到Pi9基因,仅在辽宁省农业科学院育成的辽粳421中检测到Pi40基因。参试的水稻品种中共有62个基因组合类型。基于抗性基因检测结果进行聚类分析,可以将260份粳稻材料分为11个类群。9个抗性单基因系中,Pi40抗性最好;其次是Pi9、Pigm和Pita。【结论】抗性基因Pi40、Pi9、Pigm和Pita在辽宁省粳稻品种选育和生产上具有重要的应用潜力;参试品种的80.38%集中在第Ⅴ、Ⅵ、Ⅶ和Ⅸ类群,没有明显的地域差异;参试品种抗病基因组合类型不够丰富,亲缘关系较近。
【Objective】To clarify the rice blast resistance genes in 260 japonica rice varieties in Liaoning Province,evaluate their utilization value, and provide reference for rice breeding,【Method】specific primers of nine rice blast resistance genes were selected to analyze the species, the quantity and the geographical distribution of resistance genes of260 japonica rice varieties in Liaoning, and the cluster analysis of varieties was based on the detection bands of disease resistance genes. The disease resistance of the nine resistant mongenic rice lines was evaluated by seedling-spray inoculation, natural disease investigation during the active tillering stage and ripening stage.【Result】The number of blast resistance genes in japonica rice from Liaoning varied from 0 to 7, and the frequency of Pikh gene ranked highest,followed by the Pikm and Pi1. Pi9 gene was not detected in all tested varieties, and Pi40 gene was only detected in Liaojing 421 bred by the Liaoning Academy of Agricultural Sciences. There were 62 gene combinations in the rice varieties tested, and based on the clustering analysis of the results of the resistance gene detection, 260 japonica rice materials can be divided into 11 groups. Among the nine resistant mongenic lines, Pi40 showed the best resistance,followed by Pi9, Pigm and Pita.【Conclusion】The resistance genes Pi9, Pi40, Pita, and Pigm have important application potential in breeding in Liaoning Province. 80.38% of the tested varieties concentrate in groups V, VI, VII and IX. There is no obvious geographical difference; the types of disease resistance gene combinations of the tested varieties are relatively less abundant and the genetic relationship is relatively close.
【Objective】To clarify the rice blast resistance genes in 260 japonica rice varieties in Liaoning Province,evaluate their utilization value, and provide reference for rice breeding,【Method】specific primers of nine rice blast resistance genes were selected to analyze the species, the quantity and the geographical distribution of resistance genes of260 japonica rice varieties in Liaoning, and the cluster analysis of varieties was based on the detection bands of disease resistance genes. The disease resistance of the nine resistant mongenic rice lines was evaluated by seedling-spray inoculation, natural disease investigation during the active tillering stage and ripening stage.【Result】The number of blast resistance genes in japonica rice from Liaoning varied from 0 to 7, and the frequency of Pikh gene ranked highest,followed by the Pikm and Pi1. Pi9 gene was not detected in all tested varieties, and Pi40 gene was only detected in Liaojing 421 bred by the Liaoning Academy of Agricultural Sciences. There were 62 gene combinations in the rice varieties tested, and based on the clustering analysis of the results of the resistance gene detection, 260 japonica rice materials can be divided into 11 groups. Among the nine resistant mongenic lines, Pi40 showed the best resistance,followed by Pi9, Pigm and Pita.【Conclusion】The resistance genes Pi9, Pi40, Pita, and Pigm have important application potential in breeding in Liaoning Province. 80.38% of the tested varieties concentrate in groups V, VI, VII and IX. There is no obvious geographical difference; the types of disease resistance gene combinations of the tested varieties are relatively less abundant and the genetic relationship is relatively close.
引文
[1]李跃东,邱福林,王彦荣,王之旭.辽宁省水稻育种研究现状及发展思路.北方水稻,2009,39(2):69-73.Li Y D,Qiu F L,Wang Y R,Wang Z X.Current situation of Liaoning rice breeding research and development approach.North Rice,2009,39(2):69-73.(in Chinese)
[2]赵一洲,李正茂,路洪彪,倪善君,张战,李鑫,毛艇,张丽丽,刘研.辽宁省水稻骨干亲本演变及遗传多样性分析.河南农业科学,2014,43(12):28-33.Zhao Y Z,Li Z M,Lu H B,Ni S J,Zhang Z,Li X,Mao T,Zhang L L.Evolution and genetic diversity of rice founder parents in Liaoning province.J Henan Agric Sci,2014,43(12):28-33.(in Chinese)
[3]马慧,李丽丽,祝红艳,郭志富,陈丽静,张丽,钟鸣,邵国军.辽宁稻区主要粳稻骨干亲本的遗传多样性分析.沈阳农业大学学报,2012,43(4):478-481.Ma H,Li L L,Zhu H Y,Guo Z F,Chen L J,Zhang L,Zhong M,Shao G J.Japonica rice backbone parental genetic diversity in Liaoning Province.J Shenyang Agric Univ,2012,43(4):478-481.(in Chinese with English abstract)
[4]马军韬,张国民,辛爱华,肖佳雷,刘迎雪,宫秀杰,雷财林,高玉凤.黑龙江省水稻品种对稻瘟病的抗性分析及评价利用.中国农学通报,2008,24(2):332-334.Ma J T,Zhang G M,Xin A H,Xiao J L,Liu Y X,Gong XJ,Lei C L,Gao Y F.Identification and evaluation of resistance of rice to Magnaporthe grisea in Heilongjiang Province.Chin Agric Sci Bull,2008,24(2):332-334.(in Chinese with English abstract)
[5]王妍,郑文静,王辉,张丽霞,王世维,赵家铭,刘志恒.辽宁省主栽水稻品种抗稻瘟病基因的鉴定及分析.植物遗传资源学报,2015,16(3):640-648.Wang Y,Zheng W J,Wang H,Zhang L X,Wang S W,Zhao J M,Liu Z H.Identification and analysis of rice blast resistance gene alleles in 24 rice cultivars from Liaoning Province.J Plant Genet Resour,2015,16(3):640-648.(in Chinese with English abstract)
[6]程式华,李建.现代中国水稻.北京:金盾出版社,2007.Cheng S H,Li J.Modern Chinese Rice.Beijing:Jindun Publishing House,2007
[7]王倩,周永力,王疏,黎志康.我国东北稻曲稻瘟病的研究进展.植物遗传资源学报,2012,13(1):143-147.Wang Q,Zhou Y L,Wang S,Li Z K.Research advances on the rice blast in the northeast China.J Plant Genet Resour,2012,13(1):143-147.(in Chinese with English abstract)
[8]王生轩,李俊周,谢瑛,李勇,李梦琪,曹炳武,赵全志.河南粳稻抗稻瘟病基因Pi9、Pita和Piz-t的分子检测.分子植物育种,2017,15(3):951-955.Wang S X,Li J Z,Xie Y,Li Y,Li M Q,Cao B W,Zhao QZ.Molecular detection of rice blast resistance gene Pi9,Pita and Piz-t in Henan japonica rice.Mol Plant Breed,2017,15(3):951-955.(in Chinese with English abstract)
[9]张荟,周鹏,涂诗航,郑家团,张建福,谢华安.利用分子标记辅助选择技术创制抗稻瘟病水稻新恢复系.分子植物育种,2015,13(9):1918-1922.Zhang H,Zhou P,Xu S H,Zheng J T,Zhang J F,Xie H A.Developing new restorer lines with blast-resistance gene Pi9 for hybrid rice by marker assistance selection(MAS).Mole Plant Breed,2015(9):1918-1922.(in Chinese with English abstract)
[10]杨立明,纪剑辉,周颖君,方继朝,刘永峰,罗玉明.水稻稻瘟病抗性基因Pi2-InDel标记的开发与评价.分子植物育种,2017,15(2):594-598.Yang L M,Ji J H,Zhou Y J,Fang J C,Liu Y F,Luo Y M.The development of the rice blast resistance genes Pi2-InDel markers and its evaluation in application.Mol Plant Breed,2017,15(2):594-598.(in Chinese with English abstract)
[11]华丽霞,汪文娟,陈深,汪聪颖,曾烈先,杨健源,朱小源,苏菁.抗稻瘟病Pi2/9/z-t基因特异性分子标记的开发.中国水稻科学,2015,29(3):305-310.Hua L X,Wang W J,Chen S,Wang C Y,Zeng L X,Yang J Y,Zhu X Y,Su Q.Development of specific DNAmarkers for detecting the rice blast resistance gene alleles Pi2/9/z-t.Chin J Rice Sci,2015,29(3):305-310.(in Chinese with English abstract)
[12]Jeung J U,Kim B R,Cho Y C,Han S S.A novel gene,Pi40(t),linked to the DNA markers derived from NBS-LRR motifs confers broad spectrum of blast resistance in rice.Theor Appl Genet,2007,115:1163-1177.
[13]王金明,林秀云,刘晓梅,孙强,李鹏志,张三元.分子标记选择水稻抗稻瘟病基因Pi40和Pib聚合体.华北农学报,2012,27(2):218-221.Wang J M,Lin X Y,Liu X M,Sun Q,Li P Z,Zhang S Y.Selecting the pyramids of blast resistance gene Pi40 and Pib in rice assisted by molecular marker.Acta Agric Bor-Sin,2012,27(2):218-221.(in Chinese with English abstract)
[14]于苗苗,戴正元,潘存红,陈夕军,余玲,张晓祥,李育红,肖宁,龚红兵,盛生兰,潘学彪,张洪熙,李爱宏.广谱稻瘟病抗性基因Pigm和Pi2的抗性频率差异及与Pi1的互作效应.作物学报,2013,39(11):1927-1934.Yu M M,Dai Z Y,Pan C J,Yu L,Zhang X X,Li Y H,Xiao N,Gong H B,Sheng S L,Pan X B,Zhang H X,Li A H.Resistance spectrum difference between two broad-spectrum blast resistance genes,Pigm and Pi2,and their interaction effect on Pi1.Acta Agron Sin,2013,39(11):1927-1934.(in Chinese with English abstract)
[15]戴小军,杨远柱,周亮,梁满中,胡小淳,陈良碧.抗稻瘟病水稻资源抗性基因Pita、Pib、Pi9以及Pikm的分布研究.生命科学研究,2012,16(4):340-344,356.Dai X J,Yang Y Z,Zhou L,Liang M Z,Hu X C,Chen LB.Distribution research of blast resistance genes Pita,Pib,Pi9 and Pikm in blast-resistant rice resources.Life Sci Res,2012,16(4):340-344,356.(in Chinese with English abstract)
[16]Jia Y L,Wang Z H,Singh P.Development of dominant rice blast Pi-ta resistance gene markers.Crop Sci,2002,42:2145-2149.
[17]Fjellstrom R,Conaway-Bormans C A,Mcclung A M.Development of DNA markers suitable for marker assisted selection of three Pi genes conferring resistance to multiple Pyricularia grisea pathotypes.Crop Sci,2004,44:1790-1798.
[18]刘洋,徐培洲,张红宇,徐建第,吴发强,吴先军.水稻抗稻瘟病Pib基因的分子标记辅助选择与应用.中国农业科学,2008,41(1):9-14.Liu Y,Xu P Z,Zhang H Y,Xu J D,Wu F Q,Wu X J.Marker-assisted selection and application of blast resistant gene Pib in rice.Sci Agric Sin,2008,41(1):9-14.(in Chinese with English abstract)
[19]李洪亮,李荣田.稻瘟病抗性基因Pi1和Pi2的聚合及其育种价值分析.北方水稻,2010,40(5):7-12.Li H L,Li R T.The polymerization of rice-blast resistance genes Pi1 and Pi2 and analysis on breeding value.North Rice,2010,40(5):7-12.(in Chinese)
[20]Zhai C,Lin F,Dong Z Q,He X Y,Yuan B,Zeng X S,Wang L,Pan Q H.The isolation and characterization of Pik a rice blast resistance gene which emerged after rice domestication.New Phytol,2011,189(1):321-334.
[21]汪文娟,周继勇,汪聪颖,苏菁,封金奇,陈炳,冯爱卿,杨健源,陈深,朱小源.八个抗稻瘟病基因在华南籼型杂交水稻中的分布.中国水稻科学,2017,31(3):299-306.Wang W J,Zhou J Y,Wang C Y,Su Q,Feng J Q,Chen B,Feng A Q,Yang J Y,Chen S,Zhu X Y.Distribution of eight rice blast resistance genes in indica hybrid rice in China.Chin J Rice Sci,2017,31(3):299-306.(in Chinese with English abstract)
[22]褚晋,何鹏飞,赵正龙,吴毅歆,何朋杰,何月秋.云南省罗平县与腾冲县两地稻瘟病菌致病类型分析.中国农学通报,2016,32(14):84-89.Chu J,He P F,Zhao Z L,Wu Y X,He P J,He Y Q.Pathotype analysis of Magnaporthe oryzae in Luoping and Tengchong of Yunnan Province.Chin Agric Sci Bull,2016,32(14):84-89.(in Chinese with English abstract)
[23]李旭升,向小娇,申聪聪,杨隆维,陈凯,王小文,邱先进,朱小源,邢丹英,徐建龙.水稻重测序核心种质资源的稻瘟病抗性鉴定与评价.作物学报,2017,43(6):795-810.Li X S,Xiang X J,Shen C C,Yang L W,Chen K,Wang X W,Qiu X J,Zhu X Y,Xing D Y,Xu J L.Identification and evaluation of blast resistance for resequenced rice core collections.Acta Agrono Sin,2017,43(6):795-810.
[24]Tian D G,Chen Z J,Chen Z Q,Zhou Y C,Wang Z H,Wang F,Chen S B.Allele-specific marker-based assessment revealed that the rice blast resistance genes Pi2 and Pi9 have not been widely deployed in Chinese indica rice cultivars.Rice,2016,9(1):19.
[25]刘士平,李信,汪朝阳,李香花,何予卿.基因聚合对水稻稻瘟病的抗性影响.分子植物育种,2003,1(1):22-26.Liu S P,Li X,Wang C Y,Li X H,He Y Q.Gene pyramiding to increase the blast resistance in rice.Mol Plant Breed,2003,1(1):22-26.
[26]吴宪,Kim D R,刘晓梅,王继春,王东元,朱峰,任金平.水稻抗稻瘟病广谱基因型鉴定及稻瘟病菌生理小种型研究.吉林农业大学学报,2017,39(4):403-408.Wu X,Kim D R,Liu X M,Wang J C,Wang D Y,Zhu F,Ren J P.Identification of rice broad-spectrum genes resistant to rice blast and study on physiological races of rice blast fungal type.J Jilin Agric Univ,2017,39(4):403-408.(in Chinese with English abstract)
[27]翟荣荣,叶胜海,朱国富,余鹏,王俊梅,张小明.浙江省10个晚粳稻品种SSR指纹图谱的构建及遗传差异分析.分子植物育种,2017,15(3):944-950.Zhai R R,Ye S H,Zhu G F,Yu P,Wang J M,Zhang X M.Establishment of SSR fingerprint map and analysis of genetic diversity among the 10 Zhejiang photosensitive japonica rice cultivars.Mol Plant Breed,2017,15(3):944-950.(in Chinese with English abstract)
[28]张云,龙云星,奎丽梅,徐高峰,涂建,辜琼瑶,李华慧,黄平,卢义宣.我国水稻两用核不育系SSR遗传多样性分析.分子植物育种,2017,15(7):2836-2846.Zhang Y,Long Y X,Kui L M,Xu G F,X J,Gu Q Y,Li HH,Huang P,Lu Y X.SSR analysis on genetic diversity of rice dual-use nuclear male sterile lines in China.Mol Plant Breed,2017,15(7):2836-2846.(in Chinese with English abstract)
[29]聂元元,李霞,毛凌华,颜满莲,颜龙安,蔡耀辉.分子标记辅助选择改良三系杂交稻恢复系R225稻瘟病抗性.中国稻米,2016,22(3):60-63.Nie Y Y,Li X,Mao L H,Yan M L,Yan L A,Cai Y H.Improving blast resistance of parental restorer lines R225by marker-assisted selection.China Rice,2016,22(3):60-63.(in Chinese)
[30]张晓慧,冯晓敏,林少扬.水稻主栽品种空育131抗稻瘟病位点的扫描及其基因组重构建.植物学报,2017,52(1):30-42.Zhang X H,Feng X M,Lin S Y.Scanning for Pi loci and rebuilding an improved genome of elite rice variety Kongyu 131.Chin Bull Bot,2017,52(1):30-42.(in Chinese with English abstract)
[2]赵一洲,李正茂,路洪彪,倪善君,张战,李鑫,毛艇,张丽丽,刘研.辽宁省水稻骨干亲本演变及遗传多样性分析.河南农业科学,2014,43(12):28-33.Zhao Y Z,Li Z M,Lu H B,Ni S J,Zhang Z,Li X,Mao T,Zhang L L.Evolution and genetic diversity of rice founder parents in Liaoning province.J Henan Agric Sci,2014,43(12):28-33.(in Chinese)
[3]马慧,李丽丽,祝红艳,郭志富,陈丽静,张丽,钟鸣,邵国军.辽宁稻区主要粳稻骨干亲本的遗传多样性分析.沈阳农业大学学报,2012,43(4):478-481.Ma H,Li L L,Zhu H Y,Guo Z F,Chen L J,Zhang L,Zhong M,Shao G J.Japonica rice backbone parental genetic diversity in Liaoning Province.J Shenyang Agric Univ,2012,43(4):478-481.(in Chinese with English abstract)
[4]马军韬,张国民,辛爱华,肖佳雷,刘迎雪,宫秀杰,雷财林,高玉凤.黑龙江省水稻品种对稻瘟病的抗性分析及评价利用.中国农学通报,2008,24(2):332-334.Ma J T,Zhang G M,Xin A H,Xiao J L,Liu Y X,Gong XJ,Lei C L,Gao Y F.Identification and evaluation of resistance of rice to Magnaporthe grisea in Heilongjiang Province.Chin Agric Sci Bull,2008,24(2):332-334.(in Chinese with English abstract)
[5]王妍,郑文静,王辉,张丽霞,王世维,赵家铭,刘志恒.辽宁省主栽水稻品种抗稻瘟病基因的鉴定及分析.植物遗传资源学报,2015,16(3):640-648.Wang Y,Zheng W J,Wang H,Zhang L X,Wang S W,Zhao J M,Liu Z H.Identification and analysis of rice blast resistance gene alleles in 24 rice cultivars from Liaoning Province.J Plant Genet Resour,2015,16(3):640-648.(in Chinese with English abstract)
[6]程式华,李建.现代中国水稻.北京:金盾出版社,2007.Cheng S H,Li J.Modern Chinese Rice.Beijing:Jindun Publishing House,2007
[7]王倩,周永力,王疏,黎志康.我国东北稻曲稻瘟病的研究进展.植物遗传资源学报,2012,13(1):143-147.Wang Q,Zhou Y L,Wang S,Li Z K.Research advances on the rice blast in the northeast China.J Plant Genet Resour,2012,13(1):143-147.(in Chinese with English abstract)
[8]王生轩,李俊周,谢瑛,李勇,李梦琪,曹炳武,赵全志.河南粳稻抗稻瘟病基因Pi9、Pita和Piz-t的分子检测.分子植物育种,2017,15(3):951-955.Wang S X,Li J Z,Xie Y,Li Y,Li M Q,Cao B W,Zhao QZ.Molecular detection of rice blast resistance gene Pi9,Pita and Piz-t in Henan japonica rice.Mol Plant Breed,2017,15(3):951-955.(in Chinese with English abstract)
[9]张荟,周鹏,涂诗航,郑家团,张建福,谢华安.利用分子标记辅助选择技术创制抗稻瘟病水稻新恢复系.分子植物育种,2015,13(9):1918-1922.Zhang H,Zhou P,Xu S H,Zheng J T,Zhang J F,Xie H A.Developing new restorer lines with blast-resistance gene Pi9 for hybrid rice by marker assistance selection(MAS).Mole Plant Breed,2015(9):1918-1922.(in Chinese with English abstract)
[10]杨立明,纪剑辉,周颖君,方继朝,刘永峰,罗玉明.水稻稻瘟病抗性基因Pi2-InDel标记的开发与评价.分子植物育种,2017,15(2):594-598.Yang L M,Ji J H,Zhou Y J,Fang J C,Liu Y F,Luo Y M.The development of the rice blast resistance genes Pi2-InDel markers and its evaluation in application.Mol Plant Breed,2017,15(2):594-598.(in Chinese with English abstract)
[11]华丽霞,汪文娟,陈深,汪聪颖,曾烈先,杨健源,朱小源,苏菁.抗稻瘟病Pi2/9/z-t基因特异性分子标记的开发.中国水稻科学,2015,29(3):305-310.Hua L X,Wang W J,Chen S,Wang C Y,Zeng L X,Yang J Y,Zhu X Y,Su Q.Development of specific DNAmarkers for detecting the rice blast resistance gene alleles Pi2/9/z-t.Chin J Rice Sci,2015,29(3):305-310.(in Chinese with English abstract)
[12]Jeung J U,Kim B R,Cho Y C,Han S S.A novel gene,Pi40(t),linked to the DNA markers derived from NBS-LRR motifs confers broad spectrum of blast resistance in rice.Theor Appl Genet,2007,115:1163-1177.
[13]王金明,林秀云,刘晓梅,孙强,李鹏志,张三元.分子标记选择水稻抗稻瘟病基因Pi40和Pib聚合体.华北农学报,2012,27(2):218-221.Wang J M,Lin X Y,Liu X M,Sun Q,Li P Z,Zhang S Y.Selecting the pyramids of blast resistance gene Pi40 and Pib in rice assisted by molecular marker.Acta Agric Bor-Sin,2012,27(2):218-221.(in Chinese with English abstract)
[14]于苗苗,戴正元,潘存红,陈夕军,余玲,张晓祥,李育红,肖宁,龚红兵,盛生兰,潘学彪,张洪熙,李爱宏.广谱稻瘟病抗性基因Pigm和Pi2的抗性频率差异及与Pi1的互作效应.作物学报,2013,39(11):1927-1934.Yu M M,Dai Z Y,Pan C J,Yu L,Zhang X X,Li Y H,Xiao N,Gong H B,Sheng S L,Pan X B,Zhang H X,Li A H.Resistance spectrum difference between two broad-spectrum blast resistance genes,Pigm and Pi2,and their interaction effect on Pi1.Acta Agron Sin,2013,39(11):1927-1934.(in Chinese with English abstract)
[15]戴小军,杨远柱,周亮,梁满中,胡小淳,陈良碧.抗稻瘟病水稻资源抗性基因Pita、Pib、Pi9以及Pikm的分布研究.生命科学研究,2012,16(4):340-344,356.Dai X J,Yang Y Z,Zhou L,Liang M Z,Hu X C,Chen LB.Distribution research of blast resistance genes Pita,Pib,Pi9 and Pikm in blast-resistant rice resources.Life Sci Res,2012,16(4):340-344,356.(in Chinese with English abstract)
[16]Jia Y L,Wang Z H,Singh P.Development of dominant rice blast Pi-ta resistance gene markers.Crop Sci,2002,42:2145-2149.
[17]Fjellstrom R,Conaway-Bormans C A,Mcclung A M.Development of DNA markers suitable for marker assisted selection of three Pi genes conferring resistance to multiple Pyricularia grisea pathotypes.Crop Sci,2004,44:1790-1798.
[18]刘洋,徐培洲,张红宇,徐建第,吴发强,吴先军.水稻抗稻瘟病Pib基因的分子标记辅助选择与应用.中国农业科学,2008,41(1):9-14.Liu Y,Xu P Z,Zhang H Y,Xu J D,Wu F Q,Wu X J.Marker-assisted selection and application of blast resistant gene Pib in rice.Sci Agric Sin,2008,41(1):9-14.(in Chinese with English abstract)
[19]李洪亮,李荣田.稻瘟病抗性基因Pi1和Pi2的聚合及其育种价值分析.北方水稻,2010,40(5):7-12.Li H L,Li R T.The polymerization of rice-blast resistance genes Pi1 and Pi2 and analysis on breeding value.North Rice,2010,40(5):7-12.(in Chinese)
[20]Zhai C,Lin F,Dong Z Q,He X Y,Yuan B,Zeng X S,Wang L,Pan Q H.The isolation and characterization of Pik a rice blast resistance gene which emerged after rice domestication.New Phytol,2011,189(1):321-334.
[21]汪文娟,周继勇,汪聪颖,苏菁,封金奇,陈炳,冯爱卿,杨健源,陈深,朱小源.八个抗稻瘟病基因在华南籼型杂交水稻中的分布.中国水稻科学,2017,31(3):299-306.Wang W J,Zhou J Y,Wang C Y,Su Q,Feng J Q,Chen B,Feng A Q,Yang J Y,Chen S,Zhu X Y.Distribution of eight rice blast resistance genes in indica hybrid rice in China.Chin J Rice Sci,2017,31(3):299-306.(in Chinese with English abstract)
[22]褚晋,何鹏飞,赵正龙,吴毅歆,何朋杰,何月秋.云南省罗平县与腾冲县两地稻瘟病菌致病类型分析.中国农学通报,2016,32(14):84-89.Chu J,He P F,Zhao Z L,Wu Y X,He P J,He Y Q.Pathotype analysis of Magnaporthe oryzae in Luoping and Tengchong of Yunnan Province.Chin Agric Sci Bull,2016,32(14):84-89.(in Chinese with English abstract)
[23]李旭升,向小娇,申聪聪,杨隆维,陈凯,王小文,邱先进,朱小源,邢丹英,徐建龙.水稻重测序核心种质资源的稻瘟病抗性鉴定与评价.作物学报,2017,43(6):795-810.Li X S,Xiang X J,Shen C C,Yang L W,Chen K,Wang X W,Qiu X J,Zhu X Y,Xing D Y,Xu J L.Identification and evaluation of blast resistance for resequenced rice core collections.Acta Agrono Sin,2017,43(6):795-810.
[24]Tian D G,Chen Z J,Chen Z Q,Zhou Y C,Wang Z H,Wang F,Chen S B.Allele-specific marker-based assessment revealed that the rice blast resistance genes Pi2 and Pi9 have not been widely deployed in Chinese indica rice cultivars.Rice,2016,9(1):19.
[25]刘士平,李信,汪朝阳,李香花,何予卿.基因聚合对水稻稻瘟病的抗性影响.分子植物育种,2003,1(1):22-26.Liu S P,Li X,Wang C Y,Li X H,He Y Q.Gene pyramiding to increase the blast resistance in rice.Mol Plant Breed,2003,1(1):22-26.
[26]吴宪,Kim D R,刘晓梅,王继春,王东元,朱峰,任金平.水稻抗稻瘟病广谱基因型鉴定及稻瘟病菌生理小种型研究.吉林农业大学学报,2017,39(4):403-408.Wu X,Kim D R,Liu X M,Wang J C,Wang D Y,Zhu F,Ren J P.Identification of rice broad-spectrum genes resistant to rice blast and study on physiological races of rice blast fungal type.J Jilin Agric Univ,2017,39(4):403-408.(in Chinese with English abstract)
[27]翟荣荣,叶胜海,朱国富,余鹏,王俊梅,张小明.浙江省10个晚粳稻品种SSR指纹图谱的构建及遗传差异分析.分子植物育种,2017,15(3):944-950.Zhai R R,Ye S H,Zhu G F,Yu P,Wang J M,Zhang X M.Establishment of SSR fingerprint map and analysis of genetic diversity among the 10 Zhejiang photosensitive japonica rice cultivars.Mol Plant Breed,2017,15(3):944-950.(in Chinese with English abstract)
[28]张云,龙云星,奎丽梅,徐高峰,涂建,辜琼瑶,李华慧,黄平,卢义宣.我国水稻两用核不育系SSR遗传多样性分析.分子植物育种,2017,15(7):2836-2846.Zhang Y,Long Y X,Kui L M,Xu G F,X J,Gu Q Y,Li HH,Huang P,Lu Y X.SSR analysis on genetic diversity of rice dual-use nuclear male sterile lines in China.Mol Plant Breed,2017,15(7):2836-2846.(in Chinese with English abstract)
[29]聂元元,李霞,毛凌华,颜满莲,颜龙安,蔡耀辉.分子标记辅助选择改良三系杂交稻恢复系R225稻瘟病抗性.中国稻米,2016,22(3):60-63.Nie Y Y,Li X,Mao L H,Yan M L,Yan L A,Cai Y H.Improving blast resistance of parental restorer lines R225by marker-assisted selection.China Rice,2016,22(3):60-63.(in Chinese)
[30]张晓慧,冯晓敏,林少扬.水稻主栽品种空育131抗稻瘟病位点的扫描及其基因组重构建.植物学报,2017,52(1):30-42.Zhang X H,Feng X M,Lin S Y.Scanning for Pi loci and rebuilding an improved genome of elite rice variety Kongyu 131.Chin Bull Bot,2017,52(1):30-42.(in Chinese with English abstract)