水稻OsVQ20基因生物信息学及表达分析
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摘要
植物VQ蛋白作为植物体内一类重要的辅助调控因子,对植物生长发育、基因调控以及响应外界环境变化方面发挥重要作用。探讨激素及非生物胁迫下OsVQ20基因的表达模式,为水稻的品种改良提供理论依据。通过PlantCARE数据对OsVQ20基因启动子元件进行分析,利用水稻激素芯片及荧光定量PCR分析OsVQ20基因的表达模式。结果表明,OsVQ20基因启动子含有干旱(MBS)、低温(LTR)、脱落酸(ABRS)、生长素(AuxRR-core)等多种应答元件;OsVQ20基因的表达受油菜素内酯(BL)、茉莉酸(JA)的诱导,受生长素(IAA)、细胞分裂素(CTK)以及脱落酸(ABA)的抑制作用,在赤霉素(GA)处理下无明显变化。在非生物胁迫下,OsVQ20基因明显受干旱和盐胁迫的诱导,受低温和氧化胁迫的抑制,高温胁迫下无显著变化。该研究为进一步研究OsVQ20基因的生物学功能及水稻抗逆育种提供理论依据。
Plant VQ protein, as an important auxiliary regulator in plants, plays an important role in plant growth and development, gene regulation and response to environmental changes. The expression pattern of OsVQ20 gene was investigated under hormone and abiotic stress, so as to provide a theoretical basis for breed improvement of rice. The promoter elements of OsVQ20 gene were analyzed by PlantCARE data, and the expression pattern of OsVQ20 gene was analyzed by rice hormone chip and fluorescence quantitative PCR. The results showed that the promoter of OsVQ20 gene contained multiple response elements, including low temperature response element(LTR), drought responsive element(MBS), abscisic acid response element(ABRS), and auxin response element(AuxRR-core). The expression of OsVQ20 was induced by brassinolide(BL) and jasmonic acid(JA) and inhibited by IAA, cytokinin(CTK) and abscisic acid(ABA), but there was no significant changes under gibberellin treatment.Under abiotic stress, OsVQ20 gene was induced by drought and salt stress, inhibited by low temperature and oxidative stress, but had no significant change under high temperature stress. This study could lay a foundation for further study of the biological functions of OsVQ20 and breeding of rice with resistance.
Plant VQ protein, as an important auxiliary regulator in plants, plays an important role in plant growth and development, gene regulation and response to environmental changes. The expression pattern of OsVQ20 gene was investigated under hormone and abiotic stress, so as to provide a theoretical basis for breed improvement of rice. The promoter elements of OsVQ20 gene were analyzed by PlantCARE data, and the expression pattern of OsVQ20 gene was analyzed by rice hormone chip and fluorescence quantitative PCR. The results showed that the promoter of OsVQ20 gene contained multiple response elements, including low temperature response element(LTR), drought responsive element(MBS), abscisic acid response element(ABRS), and auxin response element(AuxRR-core). The expression of OsVQ20 was induced by brassinolide(BL) and jasmonic acid(JA) and inhibited by IAA, cytokinin(CTK) and abscisic acid(ABA), but there was no significant changes under gibberellin treatment.Under abiotic stress, OsVQ20 gene was induced by drought and salt stress, inhibited by low temperature and oxidative stress, but had no significant change under high temperature stress. This study could lay a foundation for further study of the biological functions of OsVQ20 and breeding of rice with resistance.
引文
Andreasson E.,Jenkins T.,Br o dersen P.,Thorgrimsen S.,Petersen N.H.,Zhu S.,Qiu J.L.,Micheelsen P.,Rocher A.,Petersen M.,Newman M.A.,Nielsen H.B.,Hirt H.,Somssich I.,Mattsson O.,and Mundy J.,2005,The MAP kinase substrate MKS1 is a regulator of plant defense responses,EMBOJ.,24(14):2579-2589
Cheng Y.,Zhou Y.,Yang Y.,Chi Y.J.,Zhou J.,Chen J.Y.,Wang F.,Fan B.F.,Shi K.,Zhou Y.H.,Yu J.Q.,and Chen Z.X.,2012,Structural and functional ana lysis of VQ motif-containing proteins in Arabidopsis as interacting proteins of WRKY transcription factors,Plant Physiol.,159(2):810
Eulgem T.,and Somssich I.E.,2007,Networks of WRKY transcription factors in defense signaling,Curr.Opin.Plant Biol.,10(4):366-371
Eulgem T.,Rushton P.J.,Robatzek S.,and Somssich I.E.,2000,The WRKY superfamily of plant transcription factors,Trends Plant Sci.,5(5):199-206
Hu Y.,Chen L.,Wang H.,Zhang L.,Wang F.,and Yu D.Q.,2013,Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance,Plant J.,74(5):730-745
Li N.,Li X.,Xiao J.,and Wang S.,2014,Comprehensive analysis of VQ motif-containing gene expression in rice defense responses to three pathogens,Plant Cell Rep.,33(9):1493-1505
Morikawa K.,Shiina T.,Murakami S.,and Toyoshima Y.,2002,Novel nuclear-encoded proteins interacting with a plastid sigma factor,Sig1,in Arabidopsis thaliana,FEBS Lett.,514(2-3):300-304
Perruc E.,Charpenteau M.,Ramirez B.C.,Jauneau A.,Galaud J.P.,Ranjeva,R.,and Ranty B.,2004,A novel calmodulin-binding protein functions as a negative regulator of osmotic stress tolerance in Arabidopsis thaliana seedlings,Plant J.,38(3):410-420
Rushton P.J.,Somssich I.E.,Ringler P.,and Shen Q.J.,2010,WRKY transcription factors.,Trends Plant Sci.,15(5):247-258
Rushton P.J.,Torres J.T.,Parniske M.,Wernert P.,Hahlbrock K.,and Somssich I.E.,1996,Interaction of elicitor induced DNA-binding proteins with elicitor response elements in the promoters of parsley PR1 gene,EMBO J.,15(20):5690-5700
Ulker B.,and Somssich I.E.,2004,WRKY transcription factors:from DNA binding towards biological function,Curr.Opin.Plant Biol.,7(5):491-498
Wang M.,Alessandro V.,Wang G.,Zhong Y.,Corso M.,Cavallini E.,and Cheng Z.M.,2015,A comprehensive survey of the grapevine VQ gene family and its transcriptional correlation with WRKY proteins,Front.Plant Sci.,6(417):417
Weyhe M.,Eschenlippold L.,Pecher P.,Scheel D.,and Lee J.,2014,Ménageàtrois:the complex relationships between mitogen-activated protein kinases,WRKY transcription factors and VQ-motif-containing proteins,Plant Signaling&Behavior.,9(8):29519
Xie Y.D.,Li W.,Guo D.,Dong J.,Zhang Q.,Fu Y.,Ren D.,Peng M.,and Xia Y.,2010,The Arabidopsis gene sigma factor-binding protein 1 plays a role in the salicylate-and jasmonate-mediated defence responses,Plant Cell Environ.,33(5):828
Zhang G.Y.,Zhang Y.H.,Li J.J.,Li H.Y.,Wang F.D.,and Gao J.W.,2017,Research progress of plant VQ protein,Nongye Shengwu Jishu Xuebao(Journal of Agricultural Biotechnology),25(1):142-149(张高原,张一卉,李景娟,李化银,王凤德,高建伟,2017,植物VQ蛋白研究进展,农业生物技术学报,25(1):142-149)
Zhang X.,Li M.J.,Zhang B.,Yin X.M.,Wang M.L.,and Xia X.J.,2016,Possibility study on improving salt tolerance of rice by overexpressing PHD-finger transcription factor gene OsM-sr16,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(7):1820-1827(张新,李明娟,张斌,殷绪明,王曼玲,夏新界,2016,水稻PHD-finger转录因子基因OsMsr16增强耐盐性的可能性研究,基因组学与应用生物学,35(7):1820-1827)
Zhang Y.,and Wang L.,2005,The WRKY transcription factor superfamily:its origin in eukaryotes and expansion in plants,BMC Evol.Biol.,5(1):1
Cheng Y.,Zhou Y.,Yang Y.,Chi Y.J.,Zhou J.,Chen J.Y.,Wang F.,Fan B.F.,Shi K.,Zhou Y.H.,Yu J.Q.,and Chen Z.X.,2012,Structural and functional ana lysis of VQ motif-containing proteins in Arabidopsis as interacting proteins of WRKY transcription factors,Plant Physiol.,159(2):810
Eulgem T.,and Somssich I.E.,2007,Networks of WRKY transcription factors in defense signaling,Curr.Opin.Plant Biol.,10(4):366-371
Eulgem T.,Rushton P.J.,Robatzek S.,and Somssich I.E.,2000,The WRKY superfamily of plant transcription factors,Trends Plant Sci.,5(5):199-206
Hu Y.,Chen L.,Wang H.,Zhang L.,Wang F.,and Yu D.Q.,2013,Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance,Plant J.,74(5):730-745
Li N.,Li X.,Xiao J.,and Wang S.,2014,Comprehensive analysis of VQ motif-containing gene expression in rice defense responses to three pathogens,Plant Cell Rep.,33(9):1493-1505
Morikawa K.,Shiina T.,Murakami S.,and Toyoshima Y.,2002,Novel nuclear-encoded proteins interacting with a plastid sigma factor,Sig1,in Arabidopsis thaliana,FEBS Lett.,514(2-3):300-304
Perruc E.,Charpenteau M.,Ramirez B.C.,Jauneau A.,Galaud J.P.,Ranjeva,R.,and Ranty B.,2004,A novel calmodulin-binding protein functions as a negative regulator of osmotic stress tolerance in Arabidopsis thaliana seedlings,Plant J.,38(3):410-420
Rushton P.J.,Somssich I.E.,Ringler P.,and Shen Q.J.,2010,WRKY transcription factors.,Trends Plant Sci.,15(5):247-258
Rushton P.J.,Torres J.T.,Parniske M.,Wernert P.,Hahlbrock K.,and Somssich I.E.,1996,Interaction of elicitor induced DNA-binding proteins with elicitor response elements in the promoters of parsley PR1 gene,EMBO J.,15(20):5690-5700
Ulker B.,and Somssich I.E.,2004,WRKY transcription factors:from DNA binding towards biological function,Curr.Opin.Plant Biol.,7(5):491-498
Wang M.,Alessandro V.,Wang G.,Zhong Y.,Corso M.,Cavallini E.,and Cheng Z.M.,2015,A comprehensive survey of the grapevine VQ gene family and its transcriptional correlation with WRKY proteins,Front.Plant Sci.,6(417):417
Weyhe M.,Eschenlippold L.,Pecher P.,Scheel D.,and Lee J.,2014,Ménageàtrois:the complex relationships between mitogen-activated protein kinases,WRKY transcription factors and VQ-motif-containing proteins,Plant Signaling&Behavior.,9(8):29519
Xie Y.D.,Li W.,Guo D.,Dong J.,Zhang Q.,Fu Y.,Ren D.,Peng M.,and Xia Y.,2010,The Arabidopsis gene sigma factor-binding protein 1 plays a role in the salicylate-and jasmonate-mediated defence responses,Plant Cell Environ.,33(5):828
Zhang G.Y.,Zhang Y.H.,Li J.J.,Li H.Y.,Wang F.D.,and Gao J.W.,2017,Research progress of plant VQ protein,Nongye Shengwu Jishu Xuebao(Journal of Agricultural Biotechnology),25(1):142-149(张高原,张一卉,李景娟,李化银,王凤德,高建伟,2017,植物VQ蛋白研究进展,农业生物技术学报,25(1):142-149)
Zhang X.,Li M.J.,Zhang B.,Yin X.M.,Wang M.L.,and Xia X.J.,2016,Possibility study on improving salt tolerance of rice by overexpressing PHD-finger transcription factor gene OsM-sr16,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),35(7):1820-1827(张新,李明娟,张斌,殷绪明,王曼玲,夏新界,2016,水稻PHD-finger转录因子基因OsMsr16增强耐盐性的可能性研究,基因组学与应用生物学,35(7):1820-1827)
Zhang Y.,and Wang L.,2005,The WRKY transcription factor superfamily:its origin in eukaryotes and expansion in plants,BMC Evol.Biol.,5(1):1