外源硒对岚皋花魔芋生长发育的影响
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摘要
[目的]通过在土壤中添加不同浓度的亚硒酸钠,探究能够促进岚皋花魔芋生长发育的最适硒浓度。[方法]以安康当地的岚皋花魔芋为试验材料,在盆栽土壤中添加不同浓度的亚硒酸钠水溶液,摸索岚皋花魔芋对不同浓度硒的生理响应。[结果](1)硒浓度在6.25mg·kg~(-1)时,魔芋柄茎及叶幅均达到最大值,分别为1.88cm和50.25cm;硒浓度在6.25~25mg·kg~(-1)的范围内,株高达到最大值,为49.6cm。(2)硒浓度在25mg·kg~(-1)时,叶绿素a及总的叶绿素含量均达到最大值,分别为4.64mg·g-1和5.97mg·g-1;叶绿素b的含量随硒浓度的增加而先升后降,但各处理间差异不显著。(3)硒浓度在50 mg·kg~(-1)时,脯氨酸含量达到最大值,为86.14ug·g-1;丙二醛在硒浓度为6.25~12.5mg·kg~(-1)的范围内,含量降到最低,为0.07mmol·g-1。(4)魔芋鲜重在硒浓度为12.5~25mg·kg~(-1)的范围内达到最大值,为72.9g;魔芋KGM含量在硒浓度为6.25mg·kg~(-1)时达到最大值,为46.61%;魔芋球茎含硒量在硒浓度为200mg·kg~(-1)时达到最大,为41.54mg·kg~(-1)。[结论]魔芋在不同硒浓度下的各项生理生化指标,认为能够促进岚皋花魔芋生长发育的土壤施硒量为6.25~25mg·kg~(-1)。
[Objective]This study was conducted to investigate the effect of selenium on the growth and development of the local konjac species in Langao.[Methods]The influence of exogenous selenium at different concentrations on the developmental rate of konjac was determined by incorporating sodium selenite at different concentrations into potting soil,and parameters of developmental and physiological features were measured at different time points.[Results]Study showed that:(1)Stem diameter and leave area of plants were reached the greatest value at 1.88 cm and50.25 cm,respectively with the concentration of selenium at 6.25 mg·kg~(-1).And plants showed the highest height at49.6 cm when treated with selenium in the range of 6.25~25 mg·kg~(-1).(2)The contents of chlorophyll a and total chlorophyll reached up to 4.64 mg·g-1 and 5.97 mg·g-1,respectively when the concentration of selenium was25 mg·kg~(-1).And,the content of chlorophyll b fluctuated with the increase of selenium concentrations,but no significant difference was observed among treatments.(3)The content of proline reached the maximum value at 86.14 ug·g-1 when the concentration of selenium was 50 mg·kg~(-1).Furthermore,the content of malondialdehyde was decreased to a minimum value at0.07 mmol·g-1 when the concentration of selenium within the scope of 6.25~12.5 mg·kg~(-1).(4)The biggest fresh weight of konjac fruit at 72.9 g was produced in the range of 12.5~25 mg·kg~(-1) of selenium concentration,while KGM of konjac fruit was the highest at 46.61% with the selenium concentration at 6.25 mg·kg~(-1).Selenium content in bulbs was reached up to200 mg·kg~(-1) when selenium concentration incorporated was 41.54 mg·kg~(-1).[Conclusion]In summary,the appropriate selenium content can promote the growth of konjac,and the optimal amount of soil selenium was determined at 6.25~25 mg·kg~(-1).
[Objective]This study was conducted to investigate the effect of selenium on the growth and development of the local konjac species in Langao.[Methods]The influence of exogenous selenium at different concentrations on the developmental rate of konjac was determined by incorporating sodium selenite at different concentrations into potting soil,and parameters of developmental and physiological features were measured at different time points.[Results]Study showed that:(1)Stem diameter and leave area of plants were reached the greatest value at 1.88 cm and50.25 cm,respectively with the concentration of selenium at 6.25 mg·kg~(-1).And plants showed the highest height at49.6 cm when treated with selenium in the range of 6.25~25 mg·kg~(-1).(2)The contents of chlorophyll a and total chlorophyll reached up to 4.64 mg·g-1 and 5.97 mg·g-1,respectively when the concentration of selenium was25 mg·kg~(-1).And,the content of chlorophyll b fluctuated with the increase of selenium concentrations,but no significant difference was observed among treatments.(3)The content of proline reached the maximum value at 86.14 ug·g-1 when the concentration of selenium was 50 mg·kg~(-1).Furthermore,the content of malondialdehyde was decreased to a minimum value at0.07 mmol·g-1 when the concentration of selenium within the scope of 6.25~12.5 mg·kg~(-1).(4)The biggest fresh weight of konjac fruit at 72.9 g was produced in the range of 12.5~25 mg·kg~(-1) of selenium concentration,while KGM of konjac fruit was the highest at 46.61% with the selenium concentration at 6.25 mg·kg~(-1).Selenium content in bulbs was reached up to200 mg·kg~(-1) when selenium concentration incorporated was 41.54 mg·kg~(-1).[Conclusion]In summary,the appropriate selenium content can promote the growth of konjac,and the optimal amount of soil selenium was determined at 6.25~25 mg·kg~(-1).
引文
[1]刘佩瑛.魔芋学[M].北京:中国农业出版社,2004:8-11.
[2]王俊,黄明,徐幸莲,等.硒及富硒功能食品研究进展[J].江苏农业科学,2003(2):53-56.
[3]李秀梅.微量元素硒与人体健康[J].人参研究,2004,16(2):19-21.
[4]王浩东,张建东,谢娟平,等.安康地区土壤硒资源分布规律研究[J].安康学院学报,2013,25(6):8-11.
[5]万佐玺,易咏梅,杨兰芳,等.土壤施硒对魔芋含硒量与吸硒特性的影响[J].华中农业大学学报,2005,24(4):359-363.
[6]刘枫,刘英,何传龙,等.淮北砂姜黑土大豆施硒效应研究[J].土壤通报,2007,38(6):1246-1248.
[7]罗玉梅,吕银华,刘江燕.分光光度法测定鲜魔芋中主要成分[J].湖北师范学院学报(自然科学版),2006,26(2):65-67.
[8]李静,夏建国,巩发永,等.外源硒肥对茶叶硒含量及化学品质的影响研究[J].水土保持学报,2005,19(4):104-106,126.
[9]施和平,张英豪,刘振声.番茄对硒的吸收、分布和转化[J].植物学报(英文版),1993,35(7):541-546.
[10]尚庆茂,李式军.高浓度硒对生菜的毒害效应[C]//中国园艺学会首届青年学术研讨会论文集.北京:农业出版社,1994:511-513.
[11]薛泰麟,侯少范,谭见安,等.硒对过氧化作用的抑制效应及酶促机制的探讨[J].科学通报,1993,38(3):274-277.
[12]张福锁,李孙荣.非生物害源引起的植物受害表征与诊断[M].北京:中国农业大学出版社,1996:323-325.
[13]万洪富.生态环境中的硒及植物对它的吸收和转化[J].土壤学进展,1988,16(6):3-11,23.
[14]谭周磁,陈平,陈嘉勤,等.稻田土壤硒含量及施硒对水稻产量与米质的影响[J].湖南师范大学自然科学学报,1997,(3):62-65.
[15]周大寨,唐巧玉,张驰,等.硒对花椰菜幼苗叶绿素、脯氨酸及丙二醛含量的影响[J].湖北农业科学,2005,(2):77-78,107.
[16]王宁宁,杜晓光,朱亮基.亚硒酸钠对转绿小麦叶片内叶绿素生物合成和某些抗氧化作用的影响[J].南开大学学报(自然科学版),1994(2):21-24,46.
[17]Padmaja K,Prasad D D K,Prasad A R K.Effect of selenium on chlorophyll biosynthesis in mung bean seedlings[J].Phytochemistry,1989,28(12):3321-3324.
[18]果秀敏,牛君仿,方正,等.植物中硒的形态及其生理作用[J].河北农业大学学报,2003,26(S1):142-143,147
[19]Chen Y.Crowing Se rich tea plant[J].International Tea Quality-Human Health Symposium,1996(11):7-10.
[20]吴雄平,梁东丽,鲍俊丹,等.Se(Ⅳ)和Se(Ⅵ)对小白菜生长及生理效应的影响[J].环境科学学报,2009,29(10):2163-2171.
[21]张弛,吴永尧,彭振坤,等.硒对油菜苗期叶片色素的影响研究[J].湖北民族学院学报(自然科学版),2002,20(3):63-65.
[22]吴之琳,尹雪斌,袁林喜,等.硒在植物抗逆境胁迫耐受中的作用[J].粮食科技与经济,2014,39(3):26-31.
[23]许春霞,李向民,肖永绥.土施硒肥与茶叶含硒量和产量的关系[J].西北农业学报,1996,5(1):71-75.
[24]尚庆茂,陈淑芳,张志刚.硒对高温胁迫下辣椒叶片抗氧化酶活性的调节作用[J].园艺学报,2005,32(1):35-38.
[25]李登超,朱祝军,韩秋敏,等.硒对菠菜、小白菜生长及抗氧化活性的研究[J].上海交通大学学报(农业科学版),2003,21(1):5-8.
[26]李登超,朱祝军,徐志豪,等.硒对小白菜生长和养分吸收的影响[J].植物营养与肥料学报,2003,9(3):353-358.
[27]Chen D,Ren S,Lu W,et al.Effect of applying selenium fertilizer to improve soil and increase selenium level in food for prevention and treatment of Kaschin-Beck disease[J].Journal of Environmental Sciences,1993(3):299-309.
[28]宋家永,李敬光,王永华,等.喷施硒肥对小麦生理特性、子粒硒含量的影响[J].河南农业大学学报,2005,39(2):139-142.
[29]殷金岩,耿增超,李致颖,等.硒肥对马铃薯硒素吸收、转化及产量、品质的影响[J].生态学报,2015,35(3):823-829.
[30]邓正春,李丕炎,喻米玲,等.维生素锌硒肥对水稻产量及品质的影响[J].中国稻米,2011,17(4):30-31.
[31]唐巧玉,吴永尧,周毅峰.硒对大豆生长和某些生理生化指标的影响[J].植物生理学通讯,2005,41(1):53.
[32]郝玉波,刘华琳,慈晓科,等.施硒对两种类型玉米硒元素分配及产量、品质的影响[J].应用生态学报,2012,23(2):411-418.
[33]铁梅,韩杰,李宝瑞,等.土壤施硒对燕麦硒含量及产量的影响[J].中国农业大学学报,2015,20(5):74-80.
[34]后丹.魔芋对硒的生理响应和硒对种子萌发与土壤酶活性的影响[D].武汉:湖北大学,2012.
[35]后丹,杨兰芳,余同玲.魔芋在不同土壤环境条件的含硒量[J].湖北大学学报(自然科学版),2012,34(2):202-207.
[36]许春霞,李向民,肖永绥.土施硒肥与茶叶含硒量和产量的关系[J].西北农业学报,1996,5(1):71-75.
[37]万佐玺,易咏梅,杨兰芳,等.土壤施硒对魔芋含硒量与吸硒特性的影响[J].华中农业大学学报,2005,24(4):359-363.
[38]万佐玺,易咏梅,孙益军,等.土壤施硒对白肋烟含硒量的影响[J].中国烟草学报,2004,10(6):25-28.
[39]段晓琴,赵峥嵘.硒肥对大白菜含硒量及品质的影响[J].江苏农业科学,2012,40(5):126-128.
[40]郭全忠.土壤含硒量对四种蔬菜产量和硒累积量的影响[J].陕西农业科学,2014,60(11):33-35.
[2]王俊,黄明,徐幸莲,等.硒及富硒功能食品研究进展[J].江苏农业科学,2003(2):53-56.
[3]李秀梅.微量元素硒与人体健康[J].人参研究,2004,16(2):19-21.
[4]王浩东,张建东,谢娟平,等.安康地区土壤硒资源分布规律研究[J].安康学院学报,2013,25(6):8-11.
[5]万佐玺,易咏梅,杨兰芳,等.土壤施硒对魔芋含硒量与吸硒特性的影响[J].华中农业大学学报,2005,24(4):359-363.
[6]刘枫,刘英,何传龙,等.淮北砂姜黑土大豆施硒效应研究[J].土壤通报,2007,38(6):1246-1248.
[7]罗玉梅,吕银华,刘江燕.分光光度法测定鲜魔芋中主要成分[J].湖北师范学院学报(自然科学版),2006,26(2):65-67.
[8]李静,夏建国,巩发永,等.外源硒肥对茶叶硒含量及化学品质的影响研究[J].水土保持学报,2005,19(4):104-106,126.
[9]施和平,张英豪,刘振声.番茄对硒的吸收、分布和转化[J].植物学报(英文版),1993,35(7):541-546.
[10]尚庆茂,李式军.高浓度硒对生菜的毒害效应[C]//中国园艺学会首届青年学术研讨会论文集.北京:农业出版社,1994:511-513.
[11]薛泰麟,侯少范,谭见安,等.硒对过氧化作用的抑制效应及酶促机制的探讨[J].科学通报,1993,38(3):274-277.
[12]张福锁,李孙荣.非生物害源引起的植物受害表征与诊断[M].北京:中国农业大学出版社,1996:323-325.
[13]万洪富.生态环境中的硒及植物对它的吸收和转化[J].土壤学进展,1988,16(6):3-11,23.
[14]谭周磁,陈平,陈嘉勤,等.稻田土壤硒含量及施硒对水稻产量与米质的影响[J].湖南师范大学自然科学学报,1997,(3):62-65.
[15]周大寨,唐巧玉,张驰,等.硒对花椰菜幼苗叶绿素、脯氨酸及丙二醛含量的影响[J].湖北农业科学,2005,(2):77-78,107.
[16]王宁宁,杜晓光,朱亮基.亚硒酸钠对转绿小麦叶片内叶绿素生物合成和某些抗氧化作用的影响[J].南开大学学报(自然科学版),1994(2):21-24,46.
[17]Padmaja K,Prasad D D K,Prasad A R K.Effect of selenium on chlorophyll biosynthesis in mung bean seedlings[J].Phytochemistry,1989,28(12):3321-3324.
[18]果秀敏,牛君仿,方正,等.植物中硒的形态及其生理作用[J].河北农业大学学报,2003,26(S1):142-143,147
[19]Chen Y.Crowing Se rich tea plant[J].International Tea Quality-Human Health Symposium,1996(11):7-10.
[20]吴雄平,梁东丽,鲍俊丹,等.Se(Ⅳ)和Se(Ⅵ)对小白菜生长及生理效应的影响[J].环境科学学报,2009,29(10):2163-2171.
[21]张弛,吴永尧,彭振坤,等.硒对油菜苗期叶片色素的影响研究[J].湖北民族学院学报(自然科学版),2002,20(3):63-65.
[22]吴之琳,尹雪斌,袁林喜,等.硒在植物抗逆境胁迫耐受中的作用[J].粮食科技与经济,2014,39(3):26-31.
[23]许春霞,李向民,肖永绥.土施硒肥与茶叶含硒量和产量的关系[J].西北农业学报,1996,5(1):71-75.
[24]尚庆茂,陈淑芳,张志刚.硒对高温胁迫下辣椒叶片抗氧化酶活性的调节作用[J].园艺学报,2005,32(1):35-38.
[25]李登超,朱祝军,韩秋敏,等.硒对菠菜、小白菜生长及抗氧化活性的研究[J].上海交通大学学报(农业科学版),2003,21(1):5-8.
[26]李登超,朱祝军,徐志豪,等.硒对小白菜生长和养分吸收的影响[J].植物营养与肥料学报,2003,9(3):353-358.
[27]Chen D,Ren S,Lu W,et al.Effect of applying selenium fertilizer to improve soil and increase selenium level in food for prevention and treatment of Kaschin-Beck disease[J].Journal of Environmental Sciences,1993(3):299-309.
[28]宋家永,李敬光,王永华,等.喷施硒肥对小麦生理特性、子粒硒含量的影响[J].河南农业大学学报,2005,39(2):139-142.
[29]殷金岩,耿增超,李致颖,等.硒肥对马铃薯硒素吸收、转化及产量、品质的影响[J].生态学报,2015,35(3):823-829.
[30]邓正春,李丕炎,喻米玲,等.维生素锌硒肥对水稻产量及品质的影响[J].中国稻米,2011,17(4):30-31.
[31]唐巧玉,吴永尧,周毅峰.硒对大豆生长和某些生理生化指标的影响[J].植物生理学通讯,2005,41(1):53.
[32]郝玉波,刘华琳,慈晓科,等.施硒对两种类型玉米硒元素分配及产量、品质的影响[J].应用生态学报,2012,23(2):411-418.
[33]铁梅,韩杰,李宝瑞,等.土壤施硒对燕麦硒含量及产量的影响[J].中国农业大学学报,2015,20(5):74-80.
[34]后丹.魔芋对硒的生理响应和硒对种子萌发与土壤酶活性的影响[D].武汉:湖北大学,2012.
[35]后丹,杨兰芳,余同玲.魔芋在不同土壤环境条件的含硒量[J].湖北大学学报(自然科学版),2012,34(2):202-207.
[36]许春霞,李向民,肖永绥.土施硒肥与茶叶含硒量和产量的关系[J].西北农业学报,1996,5(1):71-75.
[37]万佐玺,易咏梅,杨兰芳,等.土壤施硒对魔芋含硒量与吸硒特性的影响[J].华中农业大学学报,2005,24(4):359-363.
[38]万佐玺,易咏梅,孙益军,等.土壤施硒对白肋烟含硒量的影响[J].中国烟草学报,2004,10(6):25-28.
[39]段晓琴,赵峥嵘.硒肥对大白菜含硒量及品质的影响[J].江苏农业科学,2012,40(5):126-128.
[40]郭全忠.土壤含硒量对四种蔬菜产量和硒累积量的影响[J].陕西农业科学,2014,60(11):33-35.