芦丁通过Notch信号通路抑制结肠癌细胞增殖
摘要
目的基于Notch信号通路探讨芦丁对结肠癌细胞增殖的影响。方法采用免疫组化技术对比人结肠癌组织及癌旁正常组织中Notch-1蛋白的表达情况;采用不同浓度芦丁作用于SW480细胞,检测其对结肠癌细胞增殖及Notch-1、Hes-1表达水平的影响。结果与0μmol/L比较,芦丁0、20、40μmol/L浓度组Notch-1、Hes-1 m RNA及蛋白表达水平显著降低(均P<0. 01)。结论人结肠癌组织中Notch-1的表达明显增加,芦丁可以显著抑制SW480细胞的增殖,显著下调SW480细胞中Notch-1、Hes-1的m RNA和蛋白表达水平。
引文
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3 Mumm JS,Kopan R.Notch signaling:from the outside in[J].Devel Biol,2000;228(2):151-65.
4 Kovall RA.More complicated than it looks:assembly of Notch pathway transcription complexes[J].Oncogene,2008;27(38):5099-109.
5 Ellisen LW,Bird J,West DC,et al.TAN-1,the human homolog of the drosophila notch gene,is broken by chromosomal translocations in Tlymphoblastic neoplasms[J].Cell,1991;66(4):649-61.
6 Weng AP,Ferrando AA,Lee W,et al.Activating mutations of NOTCH1 in human T cell acute lymphoblastic leukemia[J].Science,2004;306(5694):269-71.
7 Zweidler-McKay PA,He Y,Xu L,et al.Notch signaling is a potent inducer of growth arrest and apoptosis in a wide range of B-cell malignancies[J].Blood,2005;106(12):3898-906.
8 Ranganathan P,Weaver KL,Capobianco AJ.Notch signalling in solid tumours:a little bit of everything but not all the time[J].Nat Rev Cancer,2011;11(5):338-51.
9 Nicolas M,Wolfer A,Raj K,et al.Notch1 functions as a tumor suppressor in mouse skin[J].Nat Gene,2003;33(3):416-21.
10 Mandinova A,Lefort K,Di Vignano AT,et al.The Fox O3a gene is a key negative target of canonical Notch signalling in the keratinocyte UVB response[J].EMBO J,2008;27(8):1243-54.
11 Riccio O,Van Gijn ME,Bezdek AC,et al.Loss of intestinal crypt progenitor cells owing to inactivation of both Notch1 and Notch2 is accompanied by derepression of CDK inhibitors p27 Kip1 and p57Kip2[J].EMBO Rep,2008;9(4):377-83.
12 Stransky N,Egloff AM,Tward AD,et al.The mutational landscape of head and neck squamous cell carcinoma[J].Science,2011;333(6046):1157-60.
13 Takebe N,Harris PJ,Warren RQ,et al.Targeting cancer stem cells by inhibiting Wnt,Notch,and Hedgehog pathways[J].Nat Rev Clin Oncol,2011;8(2):97-106.
14龙全江,杨韬.芦丁的研究概况及展望[J].中国中医药信息杂志,2002;9(4):39-42.
15陈志武,宋必卫,方明,等.芸香甙抗氧化作用的初步研究[J].中国药理学通报,1995;11(1):75-7.
16王丽,张丽,康文艺,等.芦丁清除DPPH自由基分光光度法与微量法抗氧化活性研究[J].中成药,2009;31(11):1785-7.
17陈文梅,金鸣,吴伟,等.芦丁抑制家兔血小板激活因子诱导血小板活化作用的实验研究[J].中国中西医结合杂志,2002;22(4):283-5.
18韩英华,秦元璋.芦丁研究现状[J].山东中医杂志,2003;22(10):635-7.
19 Gao F,Zhang YQ,Wang SC,et al.Hes1 is involved in the self-renewal and tumourigenicity of stem-like cancer cells in colon cancer[J].Sci Rep,2014;4:3963.
2 Siegel R,Ward E,Brawley O,et al.The impact of eliminating socioeconomic and racial disparities on premature cancer deaths[J].Ca Cancer J Clin,2011;61(4):212-36.
3 Mumm JS,Kopan R.Notch signaling:from the outside in[J].Devel Biol,2000;228(2):151-65.
4 Kovall RA.More complicated than it looks:assembly of Notch pathway transcription complexes[J].Oncogene,2008;27(38):5099-109.
5 Ellisen LW,Bird J,West DC,et al.TAN-1,the human homolog of the drosophila notch gene,is broken by chromosomal translocations in Tlymphoblastic neoplasms[J].Cell,1991;66(4):649-61.
6 Weng AP,Ferrando AA,Lee W,et al.Activating mutations of NOTCH1 in human T cell acute lymphoblastic leukemia[J].Science,2004;306(5694):269-71.
7 Zweidler-McKay PA,He Y,Xu L,et al.Notch signaling is a potent inducer of growth arrest and apoptosis in a wide range of B-cell malignancies[J].Blood,2005;106(12):3898-906.
8 Ranganathan P,Weaver KL,Capobianco AJ.Notch signalling in solid tumours:a little bit of everything but not all the time[J].Nat Rev Cancer,2011;11(5):338-51.
9 Nicolas M,Wolfer A,Raj K,et al.Notch1 functions as a tumor suppressor in mouse skin[J].Nat Gene,2003;33(3):416-21.
10 Mandinova A,Lefort K,Di Vignano AT,et al.The Fox O3a gene is a key negative target of canonical Notch signalling in the keratinocyte UVB response[J].EMBO J,2008;27(8):1243-54.
11 Riccio O,Van Gijn ME,Bezdek AC,et al.Loss of intestinal crypt progenitor cells owing to inactivation of both Notch1 and Notch2 is accompanied by derepression of CDK inhibitors p27 Kip1 and p57Kip2[J].EMBO Rep,2008;9(4):377-83.
12 Stransky N,Egloff AM,Tward AD,et al.The mutational landscape of head and neck squamous cell carcinoma[J].Science,2011;333(6046):1157-60.
13 Takebe N,Harris PJ,Warren RQ,et al.Targeting cancer stem cells by inhibiting Wnt,Notch,and Hedgehog pathways[J].Nat Rev Clin Oncol,2011;8(2):97-106.
14龙全江,杨韬.芦丁的研究概况及展望[J].中国中医药信息杂志,2002;9(4):39-42.
15陈志武,宋必卫,方明,等.芸香甙抗氧化作用的初步研究[J].中国药理学通报,1995;11(1):75-7.
16王丽,张丽,康文艺,等.芦丁清除DPPH自由基分光光度法与微量法抗氧化活性研究[J].中成药,2009;31(11):1785-7.
17陈文梅,金鸣,吴伟,等.芦丁抑制家兔血小板激活因子诱导血小板活化作用的实验研究[J].中国中西医结合杂志,2002;22(4):283-5.
18韩英华,秦元璋.芦丁研究现状[J].山东中医杂志,2003;22(10):635-7.
19 Gao F,Zhang YQ,Wang SC,et al.Hes1 is involved in the self-renewal and tumourigenicity of stem-like cancer cells in colon cancer[J].Sci Rep,2014;4:3963.