食源性大肠杆菌O157:H7检测方法的研究进展
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摘要
食源性病原体性质多样,这些病原体通过各种媒介进入食物,已成为威胁人群健康的重要公共卫生问题。食源性病原体的快速和特异性鉴定不仅对食品生产者而且对消费者以及食品安全行政主管部门都很重要。细菌污染造成的食物中毒严重危害着人们的健康,其中大肠杆菌O157:H7是一种病死率高的肠道传染病。为此,该文就大肠杆菌O157∶H7的传统分离鉴定法、分子生物学法和免疫学法等检测方法研究进展作一综述。
Food-borne pathogens are diverse in nature and these pathogens enter food through a variety of media and have become an important public health problem that threatens the health of the population. The rapid and specific identification of food-borne pathogens is important for not only food producers but also consumers as well as food safety authorities. Food poisoning caused by bacterial contamination seriously endangers people's health, including Escherichia coli O157:H7 is an intestinal infectious disease with high mortality rate. Therefore, the paper summarizes the detection methods of Escherichia coli O157: H7, such as traditional separation and identification method, molecular biology method and immunological method.
Food-borne pathogens are diverse in nature and these pathogens enter food through a variety of media and have become an important public health problem that threatens the health of the population. The rapid and specific identification of food-borne pathogens is important for not only food producers but also consumers as well as food safety authorities. Food poisoning caused by bacterial contamination seriously endangers people's health, including Escherichia coli O157:H7 is an intestinal infectious disease with high mortality rate. Therefore, the paper summarizes the detection methods of Escherichia coli O157: H7, such as traditional separation and identification method, molecular biology method and immunological method.
引文
[1]叶青,史明坤,刘波,等.河北省大肠杆菌O157∶H7病原学和流行病学研究[J].中国公共卫生,2000,16(7):613-614.
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[5]徐金雷,陈熔熔,张晏,等.免疫传感器在大肠杆菌O157∶H7检测中的研究进展[J].科技视界,2015(25):22-23.
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[7]GIERALTOWSKI L.Multistate Outbreak of Escherichiacoli O157∶H7Infections Linked to Dough Mix-United States,2016[J].Morb Mortal Wkly Rep,2017,66(3):88-89.
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[9]金鑫,许恒毅,谢建华,等.食源性致病大肠杆菌O157∶H7检测方法研究进展[J].食品科技,2015,40(5):339-343.
[10]王静,邱茂锋,林修光.食品中大肠杆菌O157∶H7快速分离培养方法的研究[J].卫生研究,2002,31(1):44-46.
[11]吴艳敏.PCR技术在食品检验中的应用[J].黑龙江科技信息,2014(4):77.
[12]MENG J,ZHAO S,DOYLE MP.Virulence genes of Shiga toxinproducing Escherichiacoli isolatedfrom food,animals and humans[J].Int J of Food Microbiol,1998,45(3):229-235.
[13]HASAN MS,YOUSIF AA,ALWAN MJ.Detection of Virulent Genes in E.coli O157∶H7 Isolated from Puppies and Adult Dogs by Polymerase Chain Reaction[J].Res J Vet Pract,2016,4(1):1-6.
[14]徐晓静,文思远,陈苏红,等.基因芯片技术鉴定出血性大肠杆菌O157∶H7和霍乱弧菌O139[J].军事医学科学院院刊,2006,30(2):122-126.
[15]ZHANG D,CORONEL-AGUILERA CP,ROMERO PL,et al.The Use of a Novel Nano Luc-Based Reporter Phage for the Detection of Escherichia coli O157∶H7[J/OL].Sci Rep,2016,6:33235.https://www.researchgate.net/publication/308134057_The_Use_of_a_Novel_Nano Luc_-Based_Reporter_Phage_for_the_Detection_of_Escherichia_coli_O157H7.DOI:10.1038/srep33235.
[16]刘慧玲,吕敬章,朱智壕,等.生物传感器检测食源性大肠杆菌O157∶H7研究新进展[J].食品安全质量检测学报,2013,4(6):1828-1834.
[17]GUO Q,HAN JJ,SHAN S,et al.DNA-based hybridization chain reaction and biotin-streptavidin signal amplification for sensitive detection of Escherichia coli O157:H7 through ELISA[J/OL].Biosens Bioelectron,2016,86:990-995.https://www.researchgate.net/publication/305385518_DNA-based_hybridization_chain_reaction_and_biotin-streptavidin_signal_amplification_for_sensitive_etection_of_Escherichia_coli_O157H7_through_ELISA.DOI:10.1016/j.bios.2016.07.049.
[18]WANG L,WANG R,KONG BW,et al.B cells Using Calcium Signaling for Specific and Rapid Detection of Escherichia coli O157:H7[J/OL].Sci Rep,2015,5:10598.http://www.docin.com/p-135641 5535.html.DOI:10.1038srep10598.
[19]WU W,ZHANG J,ZHENG M,et al.An aptamer-based biosensor for colorimetric detection of Escherichia coli O157∶H7[J].PLo S ONE,2012,7(11):e48999.
[20]刘程.免疫胶体金试纸条法快速联检出血性大肠杆菌O157∶H7和鲍氏志贺氏菌[D].兰州:甘肃农业大学,2014.
[21]陈明慧.快速检测猪肉中大肠杆菌O157∶H7胶体金免疫层析试纸条的研究[D].南昌:南昌大学,2015.
[22]BALAKRISHNAN B,BARIZUDDIN S,WULIJI T,et al.A rapid and highly specific immunofluorescence method to detect Escherichia coli O157∶H7 in infected meat samples[J/OL].Int J Food Microbiol,2016,231:54-62.https://doi.org/10.1016/j.ijfoodmicro.2016.05.017.
[23]DEMIRKOL DO,TIMUR S.A sandwich-type assay based on quantum dot/aptamer bioconjugates for analysis of E.coli O157∶H7 in microtiter plate format[J].Int J of Polym Mater Polym Biomater,2016,65(2):85-90.
[24]梁文斌.基于纳米材料为载体的免疫传感器的研究[D].重庆:西南大学,2008.
[25]GUO Y,WANG Y,LIU S,et al.Electrochemical immunosensor assay(EIA)for sensitive detection of E.coli O157∶H7 with signal amplification on a SG-PEDOT-Au NPs electrode interface[J].Analyst,2015,140(2):551-559.
[26]HASSAN ARH,DECEA,MERKOCI A.Highly sensitive and rapid determination of Escherichiacoli O157∶H7 in minced beef and water using electrocatalytic gold nanoparticle tags[J/OL].Biosens Bioelectron,2015,67:511-515.http://dx.doi.org/10.1016/j.bios.2014.09.019.
[27]KIYAMA M,SAITO R,IWANO S,et al.Multicolor bioluminescence obtained using firefly luciferin[J].Curr Top Med Chem,2016,16(24):2648-2655.
[2]方珍.应用液相蛋白芯片技术检测食源性大肠杆菌O157∶H7的研究[D].长春:吉林大学,2013.
[3]TALLEY JL,WAYADANDE AC,WASALA LP,et al.Association of Escherichia coli O157∶H7 with Filth Flies(Muscidae and Calliphoridae)Captured in Leafy Greens Fields and Experimental Transmission of E.coli O157:H7 to Spinach Leaves by House Flies(Diptera:Muscidae)[J].J Food Prot,2009,72(7):1547-1552.
[4]BACH SJ,MCALLISTER TA,VEIRA DM,et al.Transmission and control of Escherichiacoli O157:H7-A review[J].Can J Anim Sci,2002,82(4):475-490.
[5]徐金雷,陈熔熔,张晏,等.免疫传感器在大肠杆菌O157∶H7检测中的研究进展[J].科技视界,2015(25):22-23.
[6]LAIDLER MR,TOURDJMAN M,BUSER GL,et al.Escherichia coli O157:H7 infections associated with consumption of locally grown strawberries contaminated by deer[J].Clin Infect Dis,2013,57(8):1129-1134.
[7]GIERALTOWSKI L.Multistate Outbreak of Escherichiacoli O157∶H7Infections Linked to Dough Mix-United States,2016[J].Morb Mortal Wkly Rep,2017,66(3):88-89.
[8]武恩平,刘灵芝,张燕.郑州市2005年肠出血性大肠杆菌O157∶H7感染状况调查[J].现代预防医学,2006,33(12):2455-2456.
[9]金鑫,许恒毅,谢建华,等.食源性致病大肠杆菌O157∶H7检测方法研究进展[J].食品科技,2015,40(5):339-343.
[10]王静,邱茂锋,林修光.食品中大肠杆菌O157∶H7快速分离培养方法的研究[J].卫生研究,2002,31(1):44-46.
[11]吴艳敏.PCR技术在食品检验中的应用[J].黑龙江科技信息,2014(4):77.
[12]MENG J,ZHAO S,DOYLE MP.Virulence genes of Shiga toxinproducing Escherichiacoli isolatedfrom food,animals and humans[J].Int J of Food Microbiol,1998,45(3):229-235.
[13]HASAN MS,YOUSIF AA,ALWAN MJ.Detection of Virulent Genes in E.coli O157∶H7 Isolated from Puppies and Adult Dogs by Polymerase Chain Reaction[J].Res J Vet Pract,2016,4(1):1-6.
[14]徐晓静,文思远,陈苏红,等.基因芯片技术鉴定出血性大肠杆菌O157∶H7和霍乱弧菌O139[J].军事医学科学院院刊,2006,30(2):122-126.
[15]ZHANG D,CORONEL-AGUILERA CP,ROMERO PL,et al.The Use of a Novel Nano Luc-Based Reporter Phage for the Detection of Escherichia coli O157∶H7[J/OL].Sci Rep,2016,6:33235.https://www.researchgate.net/publication/308134057_The_Use_of_a_Novel_Nano Luc_-Based_Reporter_Phage_for_the_Detection_of_Escherichia_coli_O157H7.DOI:10.1038/srep33235.
[16]刘慧玲,吕敬章,朱智壕,等.生物传感器检测食源性大肠杆菌O157∶H7研究新进展[J].食品安全质量检测学报,2013,4(6):1828-1834.
[17]GUO Q,HAN JJ,SHAN S,et al.DNA-based hybridization chain reaction and biotin-streptavidin signal amplification for sensitive detection of Escherichia coli O157:H7 through ELISA[J/OL].Biosens Bioelectron,2016,86:990-995.https://www.researchgate.net/publication/305385518_DNA-based_hybridization_chain_reaction_and_biotin-streptavidin_signal_amplification_for_sensitive_etection_of_Escherichia_coli_O157H7_through_ELISA.DOI:10.1016/j.bios.2016.07.049.
[18]WANG L,WANG R,KONG BW,et al.B cells Using Calcium Signaling for Specific and Rapid Detection of Escherichia coli O157:H7[J/OL].Sci Rep,2015,5:10598.http://www.docin.com/p-135641 5535.html.DOI:10.1038srep10598.
[19]WU W,ZHANG J,ZHENG M,et al.An aptamer-based biosensor for colorimetric detection of Escherichia coli O157∶H7[J].PLo S ONE,2012,7(11):e48999.
[20]刘程.免疫胶体金试纸条法快速联检出血性大肠杆菌O157∶H7和鲍氏志贺氏菌[D].兰州:甘肃农业大学,2014.
[21]陈明慧.快速检测猪肉中大肠杆菌O157∶H7胶体金免疫层析试纸条的研究[D].南昌:南昌大学,2015.
[22]BALAKRISHNAN B,BARIZUDDIN S,WULIJI T,et al.A rapid and highly specific immunofluorescence method to detect Escherichia coli O157∶H7 in infected meat samples[J/OL].Int J Food Microbiol,2016,231:54-62.https://doi.org/10.1016/j.ijfoodmicro.2016.05.017.
[23]DEMIRKOL DO,TIMUR S.A sandwich-type assay based on quantum dot/aptamer bioconjugates for analysis of E.coli O157∶H7 in microtiter plate format[J].Int J of Polym Mater Polym Biomater,2016,65(2):85-90.
[24]梁文斌.基于纳米材料为载体的免疫传感器的研究[D].重庆:西南大学,2008.
[25]GUO Y,WANG Y,LIU S,et al.Electrochemical immunosensor assay(EIA)for sensitive detection of E.coli O157∶H7 with signal amplification on a SG-PEDOT-Au NPs electrode interface[J].Analyst,2015,140(2):551-559.
[26]HASSAN ARH,DECEA,MERKOCI A.Highly sensitive and rapid determination of Escherichiacoli O157∶H7 in minced beef and water using electrocatalytic gold nanoparticle tags[J/OL].Biosens Bioelectron,2015,67:511-515.http://dx.doi.org/10.1016/j.bios.2014.09.019.
[27]KIYAMA M,SAITO R,IWANO S,et al.Multicolor bioluminescence obtained using firefly luciferin[J].Curr Top Med Chem,2016,16(24):2648-2655.