数字PCR在食品快速检测中的应用进展
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摘要
食品安全日益成为一个重要的公共卫生问题,食源性疾病、转基因食品和食品原料造假在发达国家和发展中国家都呈现出普遍和日益增长的趋势。传统检测方法费时费力,难以满足对食品快速检测的需求,应用新型数字PCR技术针对食源性病原体、转基因食品和原料造假的方法,开发出可以满足当下食品快速和精确监测的需要,并控制和预防人类食源性感染的最有效方法之一。为此,综述了近年来数字PCR技术在食品快速检测中的应用。
Food safety has increasingly become an important public health problem. The food-borne diseases,genetically modified foods and falsification of food raw materials have become a common and growing public health problem in both the developed and developing countries. However,the traditional detection methods are time-consuming and laborious,which are insufficient to meet the demands of food rapid detection. The new digital PCR technology can be developed to detect the food-borne pathogens,genetically modified foods and false raw materials,which can meet the demands of rapid and accurate food monitoring,and it is one of the most effective methods for the control and prevention of human food-borne infections. So,the application of digital PCR technology in food rapid detection is reviewed in this paper.
Food safety has increasingly become an important public health problem. The food-borne diseases,genetically modified foods and falsification of food raw materials have become a common and growing public health problem in both the developed and developing countries. However,the traditional detection methods are time-consuming and laborious,which are insufficient to meet the demands of food rapid detection. The new digital PCR technology can be developed to detect the food-borne pathogens,genetically modified foods and false raw materials,which can meet the demands of rapid and accurate food monitoring,and it is one of the most effective methods for the control and prevention of human food-borne infections. So,the application of digital PCR technology in food rapid detection is reviewed in this paper.
引文
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[15]Fu W,Zhu P,Wang C,et al. A highly sensitive and specific method for the screening detection of genetically modified organisms based on digital PCR without pretreatment[J]. Scientific Reports,2015(5):12715.
[16] Demeke T,Grfenhan T,Holigroski,et al. Assessment of droplet digital PCR for absolute quantification of genetically engineered OXY235 canola and DP305423 soybean samples[J]. Food Control,2014(46):470-474.
[17]Corbisier P,Bhat S,Partis L,et al. Absolute quantification of genetically modified MON810 maize(Zea mays L.)by digital polymerase chain reaction[J]. Analytical and Bioanalytical Chemistry,2010,396(6):2143-2150.
[18] Morisset D,tebih D, Milavec M, et al. Quantitative analysis of food and feed samples with droplet digital PCR[J]. Plos One,2013,8(5):e62583.
[19] Dobnik D,Spilsberg B,Bogo6alec K A,et al. Multiplex quantification of 12 European Union authorized genetically modified maize lines with droplet digital polymerase chain reaction[J]. Analytical Chemistry,2015,87(16):8218-8226.
[20]Cai Y,Li X,Lv R,et al. Quantitative analysis of pork and chicken products by droplet digital PCR[J]. Biomed Research International,2014(2014):810209.
[21]Floren C,Wiedemann I,Brenig B,et al. Species identification and quantification in meat and meat products using droplet digital PCR(ddPCR)[J]. Food Chemistry,2015(173):1054-1058.
[22] Bucher T B,K9ppel R. Duplex digital droplet PCR for the determination of non-Basmati rice in Basmati rice(Oryza sativa)on the base of a deletion in the fragrant gene[J]. European Food Research and Technology,2016,242(6):927-934.
[23]Scollo F,Egea L A,Gentile A,et al. Absolute quantification of olive oil DNA by droplet digital-PCR(ddPCR):Comparison of isolation and amplification methodologies[J]. Food Chemistry,2016(213):388-394.
[24]李浩,杨冬燕,杨永存,等.运用微滴式数字PCR技术鉴定潲水油的初步研究[J].中国保健营养:中旬刊,2013(5):22-23.
[2] Dube S,Qin J,Ramakrishnan R. Mathematical analysis of copy number variation in a DVA sample using digital PCR on a nanofluidic device[J]. PLos One,2008,3(8):e2876.
[3] Sykes P J,Neoh S H,Brisco M J,et al. Quantitation of targets for PCR by use of limiting dilution[J]. Biotechniques,1992,13(3):444-449.
[4]Baker M. Digital PCR hits its stride[J]. Nat Methods,2012,9(6):541-544.
[5]Velusamy V,Arshak K,Korostynska O,et al. An overview of foodborne pathogen detection:in the perspective of biosensors[J]. Biotechnology Advances,2010,28(2):232-254.
[6]Rothrock M J,Hiett K L,Kiepper B H,et al. Quantification of zoonotic bacterial pathogens within commercial poultry processing water samples using droplet digital PCR[J]. Advances in Microbiology,2013,3(5):403-411.
[7] Bian X,Jing F,Li G,et al. A microfluidic droplet digital PCR for simultaneous detection of pathogenic Escherichia coli O157 and Listeria monocytogenes[J]. Biosensors&Bioelectronics,2015,74(4):770-777.
[8]Kim S Y,Chung B,Chang J H,et al. Simultaneous identification of 13 foodborne pathogens by using capillary electrophoresis-single strand conformation polymorphism coupled with multiplex ligation-dependent probe amplification and its application in foods[J]. Foodborne Pathogens&Disease,2016,13(10):566-574.
[9]Kelley K S,Cosman A,Belgrader P,et al. Detection of methicillin-resistant Staphylococcus aureus by a duplex droplet digital PCR assay[J]. Journal of Clinical Microbiology,2013,51(7):2033-2039.
[10]Coudray-Meunier C,Fraisse A,Martin-Latil S,et al. A comparative study of digital RT-PCR and RT-qPCR for quantification of Hepatitis A virus and Norovirus in lettuce and water samples[J]. International Journal of Food Microbiology,2015(201):17-26.
[11]Nixon G,Garson J A,Grant P,et al. Comparative study of sensitivity,linearity,and resistance to inhibition of digital and nondigital polymerase chain reaction and loop mediated isothermal amplification assays for quantification of human cytomegalovirus[J]. Analytical Chemistry,2014,86(9):4387-4394.
[12] Cremonesi P,Cortimiglia C,Picozzi C,et al. Development of a droplet digital polymerase chain reaction for rapid and simultaneous identification of common foodborne pathogens in soft cheese[J]. Frontiers in Microbiology,2016(7):1725.
[13]Burns M J,Burrell A M,Foy C A. The applicability of digital PCR for the assessment of detection limits in GMO analysis[J]. European Food Research and Technology,2010,231(3):353-362.
[14]K9ppel R,Bucher T,Frei A,et al. Droplet digital PCR versus multiplex real-time PCR method for the detection and quantification of DNA from the four transgenic soy traits MON87769,MON87708,MON87705 and FG72,and lectin[J]. European Food Research and Technology,2015,241(4):521-527.
[15]Fu W,Zhu P,Wang C,et al. A highly sensitive and specific method for the screening detection of genetically modified organisms based on digital PCR without pretreatment[J]. Scientific Reports,2015(5):12715.
[16] Demeke T,Grfenhan T,Holigroski,et al. Assessment of droplet digital PCR for absolute quantification of genetically engineered OXY235 canola and DP305423 soybean samples[J]. Food Control,2014(46):470-474.
[17]Corbisier P,Bhat S,Partis L,et al. Absolute quantification of genetically modified MON810 maize(Zea mays L.)by digital polymerase chain reaction[J]. Analytical and Bioanalytical Chemistry,2010,396(6):2143-2150.
[18] Morisset D,tebih D, Milavec M, et al. Quantitative analysis of food and feed samples with droplet digital PCR[J]. Plos One,2013,8(5):e62583.
[19] Dobnik D,Spilsberg B,Bogo6alec K A,et al. Multiplex quantification of 12 European Union authorized genetically modified maize lines with droplet digital polymerase chain reaction[J]. Analytical Chemistry,2015,87(16):8218-8226.
[20]Cai Y,Li X,Lv R,et al. Quantitative analysis of pork and chicken products by droplet digital PCR[J]. Biomed Research International,2014(2014):810209.
[21]Floren C,Wiedemann I,Brenig B,et al. Species identification and quantification in meat and meat products using droplet digital PCR(ddPCR)[J]. Food Chemistry,2015(173):1054-1058.
[22] Bucher T B,K9ppel R. Duplex digital droplet PCR for the determination of non-Basmati rice in Basmati rice(Oryza sativa)on the base of a deletion in the fragrant gene[J]. European Food Research and Technology,2016,242(6):927-934.
[23]Scollo F,Egea L A,Gentile A,et al. Absolute quantification of olive oil DNA by droplet digital-PCR(ddPCR):Comparison of isolation and amplification methodologies[J]. Food Chemistry,2016(213):388-394.
[24]李浩,杨冬燕,杨永存,等.运用微滴式数字PCR技术鉴定潲水油的初步研究[J].中国保健营养:中旬刊,2013(5):22-23.