食源性微生物的分子检测方法
|
摘要
食源性病原微生物引起的疾病是全球关注的焦点之一。尽管目前食品工业在卫生条件、食品生产、运输及存储等方面取得了不小的进展,但食源性疾病的爆发仍在继续。由于食源性病原体浓度低而难以检测,特别需要一种简单、快速、特异、灵敏、成本低而效益高的测定方法。本文就食源性微生物的分子检测方法进行阐述。
Disease caused by food-borne pathogens are one of the global concerns. Outbreaks of food-borne diseases continue despite considerable progress in sanitation, food production, transportation and storage in the food industry. Due to the low concentration of food-borne pathogens, it is difficult to detect them. In particular, a simple, rapid, specific, sensitive, low-cost and cost-effective method is needed. The molecular detection methods of food-borne microorganisms are mini-reviewed in this paper.
Disease caused by food-borne pathogens are one of the global concerns. Outbreaks of food-borne diseases continue despite considerable progress in sanitation, food production, transportation and storage in the food industry. Due to the low concentration of food-borne pathogens, it is difficult to detect them. In particular, a simple, rapid, specific, sensitive, low-cost and cost-effective method is needed. The molecular detection methods of food-borne microorganisms are mini-reviewed in this paper.
引文
[1]Abee T,van Schaik W,Siezen R J.Impact of Genomics on Microbial Food Safety[J].Trends in Biotechnology,2004,22(12):653-660.
[2]Almeida C,Azevedo N F,Fernandes R M,et al.Fluorescence in Situ Hybridization Method Using a Peptide Nucleic Acid Probe for Identification of Salmonella Spp.in a Broad Spectrum of Samples[J].Applied and Environmental Microbiology,2010,76(13):4476-4485.
[3]Hanna E S,Connor C J,Wang H H.Real-time Polymerase Chain Reaction for the Food Microbiologist:Technologies,Applications and Limitations[J].Journal of Food Science,2005,70(3):R49-R53.
[4]Wittwer C T,Hermann M G,Gundry C N,et al.Real Time Multiplex PCR Assays[J].Methods in Enzymology,2001(25):430-432.
[5]Tyagi S,Kramer F R.Molecular Beacons:Probes that Fluoresce upon Hybridization[J].Nature Biotechnology,1996(14):303-308.
[6]Suo B,He Y,Tu S I,et al.A Multiplex Real-time Polymerase Chain Reaction for Simultaneous Detection of Salmonella Spp.,Escherichia Coli O157,and Listeria Monocytogenes in Meat Products[J].Foodborne Pathogens and Disease,2010,7(6):619-628.
[7]Kim H,Kane M D,Kim S,et al.A Molecular Beacon DNAMicroarray System for Rapid Detection of E.Coli O157:H7 that Eliminates the Risk of a False Negative Signal[J].Biosensors&Bioelectronics,2007,22(6):1041-1047.
[8]Brehm-Stecher B,Young C,Jaykus L A,et al.Sample Preparation:The Forgotten Beginning[J].Journal of Food Protection,2009,72(8):1774-1789.
[2]Almeida C,Azevedo N F,Fernandes R M,et al.Fluorescence in Situ Hybridization Method Using a Peptide Nucleic Acid Probe for Identification of Salmonella Spp.in a Broad Spectrum of Samples[J].Applied and Environmental Microbiology,2010,76(13):4476-4485.
[3]Hanna E S,Connor C J,Wang H H.Real-time Polymerase Chain Reaction for the Food Microbiologist:Technologies,Applications and Limitations[J].Journal of Food Science,2005,70(3):R49-R53.
[4]Wittwer C T,Hermann M G,Gundry C N,et al.Real Time Multiplex PCR Assays[J].Methods in Enzymology,2001(25):430-432.
[5]Tyagi S,Kramer F R.Molecular Beacons:Probes that Fluoresce upon Hybridization[J].Nature Biotechnology,1996(14):303-308.
[6]Suo B,He Y,Tu S I,et al.A Multiplex Real-time Polymerase Chain Reaction for Simultaneous Detection of Salmonella Spp.,Escherichia Coli O157,and Listeria Monocytogenes in Meat Products[J].Foodborne Pathogens and Disease,2010,7(6):619-628.
[7]Kim H,Kane M D,Kim S,et al.A Molecular Beacon DNAMicroarray System for Rapid Detection of E.Coli O157:H7 that Eliminates the Risk of a False Negative Signal[J].Biosensors&Bioelectronics,2007,22(6):1041-1047.
[8]Brehm-Stecher B,Young C,Jaykus L A,et al.Sample Preparation:The Forgotten Beginning[J].Journal of Food Protection,2009,72(8):1774-1789.