二烯丙基二硫化物体内抗鸡马立克氏病毒的研究
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摘要
为探究大蒜中抗病毒的活性成分二烯丙基二硫化物(Diallyl disulfide,DADS)在体内抗鸡马立克氏病毒(Marek's Disease Virus,MDV)的效果,以弱毒疫苗株CVI988/Rispens接种鸡胚成纤维细胞(CEFS)获取大量的MDV,感染雏鸡建立模型。主要通过病理组织切片的观察、血清生化指标的检测以及实时荧光定量QPCR等方法,探讨DADS在鸡体内抗鸡马立克氏病毒的有效浓度范围以及作用效果。结果表明:DADS浓度为250,500,750μmol/m L时能有效降低雏鸡血清中天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)以及甘油三酯(TG)的水平,同时也有降低病毒拷贝数的作用。所以3种浓度的DADS能在疾病早期有效抑制患马立克氏病鸡肝脏肿瘤生长并具有体内抗病毒的效果。
The effect of Diallyl disulfide( DADS),an active ingredient of anti-virus in garlic,on against Marek' s Disease Virus( MDV) in vivo were investigated. Large amounts of MDV were obtained by inoculating chicken embryo fibroblasts( CEFS) with attenuated vaccine strain( CVI988/Rispens) o.A model was established by infecting chicks with the virus. The effective concentration range and effect of DADS against chicken Marek's virus in vivo determined based on the methods such as observation of pathological tissue sections,detection of serum biochemical markers,and real-time fluorescent quantitative Q-PCR. The results showed that levels of aspartate aminotransferase( AST),alanine aminotransferase( ALT),and triglyceride( TG) in serum were effectively reduced when DADS concentrations were 250,500,750 μmol/m L,and virus copy number was reduced. Therefore,three concentrations of DADS could effectively inhibit liver tumors development caused by Marek's disease virus in the early stage and had antiviral effect in vivo.
The effect of Diallyl disulfide( DADS),an active ingredient of anti-virus in garlic,on against Marek' s Disease Virus( MDV) in vivo were investigated. Large amounts of MDV were obtained by inoculating chicken embryo fibroblasts( CEFS) with attenuated vaccine strain( CVI988/Rispens) o.A model was established by infecting chicks with the virus. The effective concentration range and effect of DADS against chicken Marek's virus in vivo determined based on the methods such as observation of pathological tissue sections,detection of serum biochemical markers,and real-time fluorescent quantitative Q-PCR. The results showed that levels of aspartate aminotransferase( AST),alanine aminotransferase( ALT),and triglyceride( TG) in serum were effectively reduced when DADS concentrations were 250,500,750 μmol/m L,and virus copy number was reduced. Therefore,three concentrations of DADS could effectively inhibit liver tumors development caused by Marek's disease virus in the early stage and had antiviral effect in vivo.
引文
[1] Bencherit D,Remy Sylvie,Le Vern Yves,et al. Induction of DNA damages upon Marek's disease virus infection:Implication in viral replication and pathogenesis[J]. J Virol,2017,91(24):UNSP e01658-17.
[2] Nazerian K.Oncogenesis of Marek's disease[J].Cancer Res,1973,33(6):1427-1430.
[3] Mcpherson M C,Delany M E. Virus and host genomic,molecular,and cellular interactions during Marek's disease pathogenesis and oncogenesis[J]. Poult Sci,2016,95(2):412-429.
[4]孙百明.马立克氏病的免疫及疫苗研究进展[J].中国预防兽医报,2011,33(12):988-991.
[5] Gong Z H,Zhang L J,Lu P,et al.Observation on Viral Variation and Vaccine Development in Marek s Disease in China[J]. J Anim Vet Adv,2012,11(24):4611-4614.
[6] Boodhoo N,Gurung A,Sharif S,et al.Marek’s disease in chickens:a review with focus on immunology[J]. Vet Res,2016,47(1):119.
[7]马丽娜,李峰杰,陈坚,等.大蒜主要活性成分及药理作用研究进展[J].中国药理学通报,2014,30(6):760-763.
[8] Bhattacherjee D,Basu C,Bhardwaj Q,et al. Design,Synthesis and Anti-Cancer Activities of Benzyl Analogues of GarlicDerived Diallyl Disulfide(DADS)and the Corresponding Diselenides[J].Chemistryselect,2017,2(24):7399-7406.
[9] Iciek M,Kwiecien I,Wlodek L. Biological properties of garlic and garlic-derived organosulfur compounds[J]. Environ Mol Mutagen,2009,50(3):247-265.
[10] Tsao S M,Yin M C. In-vitro antimicrobial activity of four diallyl sulphides occurring naturally in garlic and Chinese leek oils[J].J Med Microbiol,2001,50(7):646-649.
[11] Bordia A,Verma S K,Srivastava K C.Effect of garlic(Allium sativum)on blood lipids,blood sugar,fibrinogen and fibrinolytic activity in patients with coronary artery disease[J].Prostag Leukotr Ess,1998,58(4):257-263.
[12] Read A F,Baigent S J,Powers C,et al.Imperfect vaccination can enhance the transmission of highly virulent pathogens[J].PLoS Biology,2015,13(7):e1002198.
[13]刘群,段亮亮,江平康.中药复方抗鸡新城疫病毒作用研究[J].安徽农业科学,2010,38(20):10697-10699,10724.
[14]刘文利,赵德明.复合中草药提取物对IBD中等毒力活疫苗免疫增强作用的研究[J].中国兽药杂志,2008,42(11):9-15.
[15]佟桂玲.临床常见肝脏病理变化及疾病诊断[J].中国畜牧兽医文摘,2013,29(2):143.
[16] Kew M C.Serum aminotransferase concentration as evidence of hepatocellular damage[J].Lancet,2000,355(9204):591-592.
[17]徐建忠,史秋梅,倪耀娣,等.紫黄散对法氏囊病雏鸡免疫器官指数及血液生化指标的影响[J].中国农学通报,2009,25(22):12-16.
[18] Chow J D Y,Lawrence R T,Healy M E,et al. Genetic inhibition of hepatic acetyl-CoA carboxylase activity increases liver fat and alters global protein acetylation[J]. Mol Metabolism,2014,3(4):419-431.
[19]魏苏宁,苏雪莹,徐国恒.肝细胞甘油三酯代谢途径异常与脂肪肝[J].中国生物化学与分子生物学报,2016,32(2):123-132.
[20]满朝来,穆卫涛,赵冬雪,等.主要禽源病毒相关microRNA的研究进展[J].生物工程学报,2015,31(9):1289-1300.
[2] Nazerian K.Oncogenesis of Marek's disease[J].Cancer Res,1973,33(6):1427-1430.
[3] Mcpherson M C,Delany M E. Virus and host genomic,molecular,and cellular interactions during Marek's disease pathogenesis and oncogenesis[J]. Poult Sci,2016,95(2):412-429.
[4]孙百明.马立克氏病的免疫及疫苗研究进展[J].中国预防兽医报,2011,33(12):988-991.
[5] Gong Z H,Zhang L J,Lu P,et al.Observation on Viral Variation and Vaccine Development in Marek s Disease in China[J]. J Anim Vet Adv,2012,11(24):4611-4614.
[6] Boodhoo N,Gurung A,Sharif S,et al.Marek’s disease in chickens:a review with focus on immunology[J]. Vet Res,2016,47(1):119.
[7]马丽娜,李峰杰,陈坚,等.大蒜主要活性成分及药理作用研究进展[J].中国药理学通报,2014,30(6):760-763.
[8] Bhattacherjee D,Basu C,Bhardwaj Q,et al. Design,Synthesis and Anti-Cancer Activities of Benzyl Analogues of GarlicDerived Diallyl Disulfide(DADS)and the Corresponding Diselenides[J].Chemistryselect,2017,2(24):7399-7406.
[9] Iciek M,Kwiecien I,Wlodek L. Biological properties of garlic and garlic-derived organosulfur compounds[J]. Environ Mol Mutagen,2009,50(3):247-265.
[10] Tsao S M,Yin M C. In-vitro antimicrobial activity of four diallyl sulphides occurring naturally in garlic and Chinese leek oils[J].J Med Microbiol,2001,50(7):646-649.
[11] Bordia A,Verma S K,Srivastava K C.Effect of garlic(Allium sativum)on blood lipids,blood sugar,fibrinogen and fibrinolytic activity in patients with coronary artery disease[J].Prostag Leukotr Ess,1998,58(4):257-263.
[12] Read A F,Baigent S J,Powers C,et al.Imperfect vaccination can enhance the transmission of highly virulent pathogens[J].PLoS Biology,2015,13(7):e1002198.
[13]刘群,段亮亮,江平康.中药复方抗鸡新城疫病毒作用研究[J].安徽农业科学,2010,38(20):10697-10699,10724.
[14]刘文利,赵德明.复合中草药提取物对IBD中等毒力活疫苗免疫增强作用的研究[J].中国兽药杂志,2008,42(11):9-15.
[15]佟桂玲.临床常见肝脏病理变化及疾病诊断[J].中国畜牧兽医文摘,2013,29(2):143.
[16] Kew M C.Serum aminotransferase concentration as evidence of hepatocellular damage[J].Lancet,2000,355(9204):591-592.
[17]徐建忠,史秋梅,倪耀娣,等.紫黄散对法氏囊病雏鸡免疫器官指数及血液生化指标的影响[J].中国农学通报,2009,25(22):12-16.
[18] Chow J D Y,Lawrence R T,Healy M E,et al. Genetic inhibition of hepatic acetyl-CoA carboxylase activity increases liver fat and alters global protein acetylation[J]. Mol Metabolism,2014,3(4):419-431.
[19]魏苏宁,苏雪莹,徐国恒.肝细胞甘油三酯代谢途径异常与脂肪肝[J].中国生物化学与分子生物学报,2016,32(2):123-132.
[20]满朝来,穆卫涛,赵冬雪,等.主要禽源病毒相关microRNA的研究进展[J].生物工程学报,2015,31(9):1289-1300.