5种茶用菊不同提取方法总黄酮含量的比较
|
摘要
以芦丁为标准品,检测波长为510nm,采用比色法测定5种茶用菊不同提取方法总黄酮含量。结果表明:5种茶用菊醇提法和水提法的总黄酮含量均有显著差异,怀菊的水提法总黄酮含量高于醇提法,其余4种茶用菊均为醇提法总黄酮含量高于水提法,水提法总黄酮含量怀菊最高,滁菊次之,黄山贡菊水提的总黄酮含量最少;怀菊、滁菊和亳菊的水提总黄酮含量较高, 3者更适合做茶用菊。
The content of total flavonoids is determined with Rutin as standard by colorimetric method. The detection wavelength is 510 nm. The total flavonoids content of 5 kinds of tea chrysanthemum extracted with alcohol is significantly different from that extracted with water. The total flavonoids content of‘Huaiju' extracted with water is higher than that extracted with alcohol, and the total flavonoids content of the remaining 4 kinds of chrysanthemum are extracted with alcohol higher than that extracted with water. Among the total flavonoids extracted with water, the Huaiju content is the highest, followed by Chuju, and Huangshangongju is the least. The content of flavonoids with water from Huaiju, Chuju and Boju are the highest, and more suitable for tea.
The content of total flavonoids is determined with Rutin as standard by colorimetric method. The detection wavelength is 510 nm. The total flavonoids content of 5 kinds of tea chrysanthemum extracted with alcohol is significantly different from that extracted with water. The total flavonoids content of‘Huaiju' extracted with water is higher than that extracted with alcohol, and the total flavonoids content of the remaining 4 kinds of chrysanthemum are extracted with alcohol higher than that extracted with water. Among the total flavonoids extracted with water, the Huaiju content is the highest, followed by Chuju, and Huangshangongju is the least. The content of flavonoids with water from Huaiju, Chuju and Boju are the highest, and more suitable for tea.
引文
[1]瞿璐,王涛,董勇喆,等.菊花化学成分与药理作用的研究进展[J].药物评价研究,2015(1):98-104.
[2]谢占芳,张倩倩,朱凌佳,等.菊花化学成分及药理活性研究进展[J].河南大学学报(医学版),2015,34(4):290-300.
[3]徐文斌,郭巧生,李彦农,等.药用菊花不同栽培类型内在质量的研究[J].中国中药杂志,2005,30(21):1 645-1 648.
[4]段崇霞,张正竹.四大药用菊花功能成分的比较研究[J].安徽农业大学学报,2008,35(1):99-105.
[5]宋宏刚,裴彩云,赵韶华,等.不同产地野菊花药材中蒙花苷及总黄酮含量测定[J].中成药,2008,30(4):556.
[6]林春蕊.野菊花总黄酮的含量测定[J].广西植物,2002,22(5):467.
[7]刘金旗,沈其权,刘劲松,等.贡菊化学成分的研究[J].中国中药杂志,2001,26(8):547-548.
[8]姜洪芳,张玖,单承莺.亳菊花中黄酮类化合物的分离鉴定[J].中国野生植物资源,2008,27(5):50-52.
[9]邹文韬,张因皎,俞菊红,等.杭白菊中黄酮类化合物的分离与鉴定[J].浙江理工大学学报,2010,27(1):140-144.
[10]贾凌云,孙启时,黄顺旺.滁菊花中黄酮类化合物的分离与鉴定[J].中国药物化学杂志,2003,13(3):159-161.
[11]张海波.怀菊花多成分质量评价研究[D].郑州:河南中医学院,2015.
[12]王加文,洪建华,雷志强.菊花总黄酮提取工艺研究[J].河北化工,2010,33(12):19-22.
[13]刘利军,于艳,时维静,等.安徽省3种药用菊花总黄酮含量比较[J].安徽科技学院学报,2012(4):47-50.
[14]卫强,彭如玉,翟永欢,等.滁菊中总黄酮的大孔树脂纯化工艺研究[J].中药材,2008,31(1):147-149.
[15]贾凌云,孙启时,黄顺旺.滁菊花中黄酮类化学成分的分离与鉴定[J].中国药物化学杂志,2003,13(3):159-161.
[16]俞浩,毛斌斌,刘汉珍,等.滁菊总黄酮提取纯化工艺研究[J].安徽科技学院学报,2012(6):38-43.
[17]王桥,金月珍,朱凌佳,等.分光光度法测定两种开封菊花总黄酮含量[J].河南大学学报(医学版),2016,35(3):172-174.
[18]李冬玲.不同来源药用菊花挥发油和总黄酮含量的比较分析[J].安徽农业科学,2010,38(7):3 444-3 446.
[19]吴雪松,许浚,张铁军,等.野菊的化学成分及质量评价研究进展[J].中草药,2015,46(3):443-451.
[20]吴明侠,王晶娟,张贵君.野菊花水煎剂中七种药效组分的含量测定[J].中成药,2011,33(2):300-304.
[2]谢占芳,张倩倩,朱凌佳,等.菊花化学成分及药理活性研究进展[J].河南大学学报(医学版),2015,34(4):290-300.
[3]徐文斌,郭巧生,李彦农,等.药用菊花不同栽培类型内在质量的研究[J].中国中药杂志,2005,30(21):1 645-1 648.
[4]段崇霞,张正竹.四大药用菊花功能成分的比较研究[J].安徽农业大学学报,2008,35(1):99-105.
[5]宋宏刚,裴彩云,赵韶华,等.不同产地野菊花药材中蒙花苷及总黄酮含量测定[J].中成药,2008,30(4):556.
[6]林春蕊.野菊花总黄酮的含量测定[J].广西植物,2002,22(5):467.
[7]刘金旗,沈其权,刘劲松,等.贡菊化学成分的研究[J].中国中药杂志,2001,26(8):547-548.
[8]姜洪芳,张玖,单承莺.亳菊花中黄酮类化合物的分离鉴定[J].中国野生植物资源,2008,27(5):50-52.
[9]邹文韬,张因皎,俞菊红,等.杭白菊中黄酮类化合物的分离与鉴定[J].浙江理工大学学报,2010,27(1):140-144.
[10]贾凌云,孙启时,黄顺旺.滁菊花中黄酮类化合物的分离与鉴定[J].中国药物化学杂志,2003,13(3):159-161.
[11]张海波.怀菊花多成分质量评价研究[D].郑州:河南中医学院,2015.
[12]王加文,洪建华,雷志强.菊花总黄酮提取工艺研究[J].河北化工,2010,33(12):19-22.
[13]刘利军,于艳,时维静,等.安徽省3种药用菊花总黄酮含量比较[J].安徽科技学院学报,2012(4):47-50.
[14]卫强,彭如玉,翟永欢,等.滁菊中总黄酮的大孔树脂纯化工艺研究[J].中药材,2008,31(1):147-149.
[15]贾凌云,孙启时,黄顺旺.滁菊花中黄酮类化学成分的分离与鉴定[J].中国药物化学杂志,2003,13(3):159-161.
[16]俞浩,毛斌斌,刘汉珍,等.滁菊总黄酮提取纯化工艺研究[J].安徽科技学院学报,2012(6):38-43.
[17]王桥,金月珍,朱凌佳,等.分光光度法测定两种开封菊花总黄酮含量[J].河南大学学报(医学版),2016,35(3):172-174.
[18]李冬玲.不同来源药用菊花挥发油和总黄酮含量的比较分析[J].安徽农业科学,2010,38(7):3 444-3 446.
[19]吴雪松,许浚,张铁军,等.野菊的化学成分及质量评价研究进展[J].中草药,2015,46(3):443-451.
[20]吴明侠,王晶娟,张贵君.野菊花水煎剂中七种药效组分的含量测定[J].中成药,2011,33(2):300-304.