低温弱光对红芸豆幼苗生长及生理生化特性的影响
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摘要
【目的】通过测定红芸豆幼苗生理生化指标,研究抗低温弱光胁迫的机制,并比较两个红芸豆品种的耐低温弱光性.【方法】以红芸豆为材料,测定分析低温弱光胁迫对幼苗生长及生理生化指标的变化.【结果】低温弱光胁迫抑制幼苗的生长,其中,小红芸豆株高、茎粗、叶面积在胁迫处理7 d后较对照分别降低19%,18%和15%,‘英国大红芸豆’分别较对照降低13.2%,16%和13%.生理生化分析结果表明,低温弱光胁迫下,2个品种幼苗叶绿素含量和PSⅡ的最大光化学效率F_v/F_m在处理7 d后达到最低,‘小红芸豆’的下降幅度较为显著;超氧化物歧化酶SOD、过氧化物酶POD、过氧化氢酶CAT活性均呈现先升高后降低,且‘小红芸豆’下降幅度更大;在胁迫处理期间抗坏血酸过氧化物酶APX活性及谷胱甘肽GSH含量一直呈下降趋势.【结论】红芸豆幼苗通过抗氧化酶系统抵御低温弱光胁迫;相比‘小红芸豆’而言,‘英国大红芸豆’具有较强的低温弱光适应性.
【Objective】 To determine the physiological and biochemical indexes of red kidney bean seedlings,to study the mechanism of low temperature and week light stress resistance,and to compare the low temperature and week light resistance of two cultivars of red kidney bean varieties.【Method】 Red kidney bean was used as material to determine the changes of seedling growth and physiological and biochemical indexes under low temperature and week light stress.【Result】 Low temperature and week light stress inhibited the growth of Red kidney bean seedlings.Among them,the plant height,stem diameter,and leaf area were reduced by 19%,18%,and 15%,respectively,compared with the control after 7 days of stress treatment,and British red kidney beans was decreased by 13.2%,16% and 13%,when compared with Red kidney bean.Physiological and biochemical analysis showed that under low temperature and week light stress,the chlorophyll content of the two cultivars and the maximum photochemical efficiency of PSII,F_v/F_m,reached the minimum after 7 days treatment,and Red kidney bean decreased significant.The activity of superoxide dismutase,peroxidase and catalase increased firstly and then decreased,and Red kidney bean decreased significantly.The ascorbate peroxidase activity and glutathione content decreased during the stress.【Conclusion】 Red kidney bean seedlings are protected against low temperature and low light stress through antioxidant enzyme system.Compared to Red kidney bean,British red kidney bean has stronger adaptability to low temperature and weak light.
【Objective】 To determine the physiological and biochemical indexes of red kidney bean seedlings,to study the mechanism of low temperature and week light stress resistance,and to compare the low temperature and week light resistance of two cultivars of red kidney bean varieties.【Method】 Red kidney bean was used as material to determine the changes of seedling growth and physiological and biochemical indexes under low temperature and week light stress.【Result】 Low temperature and week light stress inhibited the growth of Red kidney bean seedlings.Among them,the plant height,stem diameter,and leaf area were reduced by 19%,18%,and 15%,respectively,compared with the control after 7 days of stress treatment,and British red kidney beans was decreased by 13.2%,16% and 13%,when compared with Red kidney bean.Physiological and biochemical analysis showed that under low temperature and week light stress,the chlorophyll content of the two cultivars and the maximum photochemical efficiency of PSII,F_v/F_m,reached the minimum after 7 days treatment,and Red kidney bean decreased significant.The activity of superoxide dismutase,peroxidase and catalase increased firstly and then decreased,and Red kidney bean decreased significantly.The ascorbate peroxidase activity and glutathione content decreased during the stress.【Conclusion】 Red kidney bean seedlings are protected against low temperature and low light stress through antioxidant enzyme system.Compared to Red kidney bean,British red kidney bean has stronger adaptability to low temperature and weak light.
引文
[1] 高晓倩.钙对夜间低温胁迫下番茄幼苗保护酶活性及超氧阴离子产生速率的影响[C]//中国园艺学会,中国工程院农业学部.中国园艺学会2010年学术年会论文摘要集,中国园艺学会,中国工程院农业学部,2014.
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[5] 李龙,王兰芬,武晶,等.普通菜豆抗旱生理特性[J].作物学报,2014,40(4):702-710.
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[13] 田维娜,赵秀文,杨林,等.温度对菜豆货架期水分损失影响的预测模型[J].中国食品学报,2012,12(1):137-140.
[14] 张国斌,郁继华,许耀照,等.低温弱光对辣椒幼苗叶绿素a荧光参数的影响[J].甘肃农业大学学报,2004,39(6):615-619.
[15] 刘春良,王兰芬,武晶,等.普通菜豆生长习性相关基因定位[J].植物遗传资源学报,2017,18(4):713-719.
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[21] 罗娅,王小蓉,张勇,等.不同提取条件对草莓果实抗氧化物质和抗氧化活性的影响[J].食品科学,2011,32(14):108-112.
[22] 刘伟,艾希珍,梁文娟.低温弱光下水杨酸对黄瓜幼苗光合作用及抗氧化酶活性的影响[J].应用生态学报,2015,20(02):441-445.
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[24] 刘栓桃,董艳敏,卢亚楠.低温弱光对两个西葫芦品种幼苗光合速率及叶绿素荧光参数的影响[J].华北农学报,2015,24(2):139-143.
[25] 黄伟,任华中,张福墁.低温弱光对番茄苗期生长和光合作用的影响[J].中国蔬菜,2012(4):15-17.
[26] 王兴银,张福漫.弱光对日光温室黄瓜光合产物分配的影响[J].中国农业大学学报,2016,5(5):34-41.
[27] 眭晓蕾,毛胜利,王立浩.弱光条件下辣椒幼苗叶片的气体交换和叶绿素荧光特性[J].园艺学报,2017,34(3):615-622.
[28] 高辉远.大豆生长发育过程中光合作用和光合效率的调节汇[D].泰安:山东农业大学,2013.
[29] 郁继华,张国斌,冯致.低温弱光对辣椒幼苗抗氧化酶活性与质膜透性的影响[J].西北植物学报,2011,31(12):2478-2483.
[30] 陈青君,范双喜,王绍辉.弱光与偏低温弱光下温室黄瓜耐性指标的研究[J].农业工程学报,2015,21(增刊):72-76.
[31] 陈青君,张福墁,王永健,等.黄瓜对低温弱光反应的生理特性研究[J].中国农业科学,2013,36(1):77-81.
[32] 付景,李潮海,赵久然,等.弱光条件下不同玉米品种净光合速率及产量和品质和比较研究[J].河南农业大学学报,2009,43(2):130-134.
[2] 贾士芳,董树亭,王空军,等.弱光胁迫对玉米产量及光合特性的影响[J].应用生态学报,2007,18(11):2456-2461.
[3] 孙园园,孙永健,陈林,等.不同播期和抽穗期弱光胁迫对杂交稻生理性状及产量的影响[J].应用生态学报,2012,23(10):2737-2744.
[4] 刘伟.红芸豆优质高产标准化栽培技术[J].种子科技,2011,29(11):38-39.
[5] 李龙,王兰芬,武晶,等.普通菜豆抗旱生理特性[J].作物学报,2014,40(4):702-710.
[6] Laloi C,Apel K.Danon A.Reactive oxygen signaling:the latest news[J].CurrOpin Plant Biol,2014,7(3):323-328
[7] Dizengremel P.Le Thiec D.Bagard M.et al.Ozone risk assessment for plants:central role of metabolism-dependent changes in reducing power[J].Environ Pollut.2014,156(I):11-15.
[8] 陶宏征,赵昶灵,李唯奇.植物对低温的光合响应[J].中国生物化学与分子生物学报,2012,28(6):501-508.
[9] Yan N,Xu X F,Wang Z D,et al .Interractive effects of temperature and lingt intensity on photosynthesis and antioxidant enzyme activity in ZizaniaIatifoliaTurcz.plants[J].Photosynthetica.2013,51(1):127-138.
[10] 马锦林.油茶耐弱光生理特性研究[D].长沙:中南林业科技大学,2012.
[11] 任丽丽,高辉远.低温弱光胁迫对野生大豆和大豆栽培种光系统功能的影响[J].植物生理与分子生物学学报,2007,33(4):333-340.
[12] 何小燕,马锦林,张日清,等.弱光胁迫对植物生长影响的研究进展[J].经济林研究,2011,29(4):131-136.
[13] 田维娜,赵秀文,杨林,等.温度对菜豆货架期水分损失影响的预测模型[J].中国食品学报,2012,12(1):137-140.
[14] 张国斌,郁继华,许耀照,等.低温弱光对辣椒幼苗叶绿素a荧光参数的影响[J].甘肃农业大学学报,2004,39(6):615-619.
[15] 刘春良,王兰芬,武晶,等.普通菜豆生长习性相关基因定位[J].植物遗传资源学报,2017,18(4):713-719.
[16] 李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[17] 韩启厚,古瑜,于海龙.菜豆组织培养及遗传转化研究进展[J].中国农学通报,2013,29(10):97-101.
[18] Yordanov I,Velikova V.Photoinhibition of photosystem I[J].Bulg J Plant Physiol,2000,26:70-92.
[19] 刘黎军.低温弱光对日光温室茄子生长及生理生化功能的影响[D].长春:吉林农业大学,2012.
[20] 陈青君,张福墁,高丽红.不同类型黄瓜苗期对亚适温弱光生理适应的差异[J].中国农学通报,2013,19(2):1-3.
[21] 罗娅,王小蓉,张勇,等.不同提取条件对草莓果实抗氧化物质和抗氧化活性的影响[J].食品科学,2011,32(14):108-112.
[22] 刘伟,艾希珍,梁文娟.低温弱光下水杨酸对黄瓜幼苗光合作用及抗氧化酶活性的影响[J].应用生态学报,2015,20(02):441-445.
[23] 周艳虹,喻景权,钱琼秋.低温弱光对黄瓜幼苗生长及抗氧化酶活性的影响[J].应用生态学报,2013,14(6):921-924.
[24] 刘栓桃,董艳敏,卢亚楠.低温弱光对两个西葫芦品种幼苗光合速率及叶绿素荧光参数的影响[J].华北农学报,2015,24(2):139-143.
[25] 黄伟,任华中,张福墁.低温弱光对番茄苗期生长和光合作用的影响[J].中国蔬菜,2012(4):15-17.
[26] 王兴银,张福漫.弱光对日光温室黄瓜光合产物分配的影响[J].中国农业大学学报,2016,5(5):34-41.
[27] 眭晓蕾,毛胜利,王立浩.弱光条件下辣椒幼苗叶片的气体交换和叶绿素荧光特性[J].园艺学报,2017,34(3):615-622.
[28] 高辉远.大豆生长发育过程中光合作用和光合效率的调节汇[D].泰安:山东农业大学,2013.
[29] 郁继华,张国斌,冯致.低温弱光对辣椒幼苗抗氧化酶活性与质膜透性的影响[J].西北植物学报,2011,31(12):2478-2483.
[30] 陈青君,范双喜,王绍辉.弱光与偏低温弱光下温室黄瓜耐性指标的研究[J].农业工程学报,2015,21(增刊):72-76.
[31] 陈青君,张福墁,王永健,等.黄瓜对低温弱光反应的生理特性研究[J].中国农业科学,2013,36(1):77-81.
[32] 付景,李潮海,赵久然,等.弱光条件下不同玉米品种净光合速率及产量和品质和比较研究[J].河南农业大学学报,2009,43(2):130-134.