不同产地金银花提取物的指纹图谱及绿原酸、木犀草苷含量测定
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摘要
通过15批不同产地金银花提取物的指纹图谱研究及其指标成分绿原酸、木犀草苷的含量测定,为合理评价金银花提取物质量提供参考.采用HPLC法建立15批金银花提取物指纹图谱,并采用外标法测定其指标成分绿原酸、木犀草苷的含量,指纹图谱共有模式的建立采用国家药典委员会"中药指纹图谱相似度评价系统软件(2012A)"进行处理分析. 15批金银花提取物指纹图谱中标定共有峰12个,确认9个,相似度高于0.94;15批金银花提取物的提取收率范围为35.60%～47.05%,绿原酸含量范围为3.33%～4.84%;木犀草苷含量范围为0.067%～0.091%.结果表明不同产地金银花提取物的指纹图谱、指标成分含量存在差异,又存在一定程度的相关性,为金银花提取物的评价及合理利用提供实验数据.
To study the fingerprint of Lonicera japonica extract from different regions,and determining the Chlorogenic acid and Galuteolin contents,which provide a reference for reasonable assessment of the quality of L. japonica extract. 15 bateches of L. japonica extract were analysed by using the HPLC method to establish the fingerprints,and the contents of chlorogenic acid and galuteolin were determined. Data analysis was performed by using professional software named Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine composed by Chinese Pharmacopoeia Committee(Version 2012A). 15 bateches of L. japonica extract were determined and the fingerprints were established,and 12 common peaks were found and the similarity was over 0.94. The extractum rate was at the range of 35.60%-47.05%,the content of chlorogenic acid was at the range of 3.33%-4.84%,and the content of galuteolin was at the range of 0.067%-0.091%. The fingerprint and indicator component contents of L. japonica extract from different regions presence obvious differences and a certain degree of correlation,which provide experimental data for quality evaluation and rational utilization of L. japonica extract.
To study the fingerprint of Lonicera japonica extract from different regions,and determining the Chlorogenic acid and Galuteolin contents,which provide a reference for reasonable assessment of the quality of L. japonica extract. 15 bateches of L. japonica extract were analysed by using the HPLC method to establish the fingerprints,and the contents of chlorogenic acid and galuteolin were determined. Data analysis was performed by using professional software named Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine composed by Chinese Pharmacopoeia Committee(Version 2012A). 15 bateches of L. japonica extract were determined and the fingerprints were established,and 12 common peaks were found and the similarity was over 0.94. The extractum rate was at the range of 35.60%-47.05%,the content of chlorogenic acid was at the range of 3.33%-4.84%,and the content of galuteolin was at the range of 0.067%-0.091%. The fingerprint and indicator component contents of L. japonica extract from different regions presence obvious differences and a certain degree of correlation,which provide experimental data for quality evaluation and rational utilization of L. japonica extract.
引文
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[20]赵君峰,张彬,张红梅,等.不同产地金银花中绿原酸及木犀草苷的快速检测[J].安徽农业科学,2015,43(15):61-62,65.
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[3]刘国强.山东省金银花资源综述[J].科技信息,2012(2):436.
[4]秦祎婷,朱艳霞,郭玉海,等.河南巨鹿金银花生产现状与发展建议[J].中国现代中药,2012,14(2):34-36.
[5]王磊,刘金贤,赵金娟,等.我国金银花种质资源概述[J].中国现代中药,2012,14(12):28-30.
[6]王燕,王智民,林丽美,等.金银花及其同属植物化学成分研究进展[J].中国中药杂志,2008,33(8):968-972.
[7]谭政委,夏伟,余永亮,等.金银花化学成分及其药理学研究进展[J].安徽农业科学,2018,46(9):26-28,123.
[8]周萍,胡俊扬,毛春芹,等.金银花高效液相指纹图谱条件优化与建立[J].时珍国医国药,2013,24(1):97-99.
[9]宋九华,张艳明,成英,等.不同产地金银花药材HPLC指纹图谱的研究[J].化学研究与应用,2018,30(5):823-827.
[10]辛华,丰杰,程若敏,等. HPLC测定不同产地金银花中绿原酸和木犀草苷[J].中国实验方剂学杂志,2011,17(2):60-63.
[11]王学军,蔡淳钊,王新军. HPLC测定不同产地金银花绿原酸和木犀草苷含量的研究[J].广西林业科学,2018,47(2):223-225.
[12]赵金娟,管仁伟,路俊仙,等. HPLC测定不同品种金银花及叶中木犀草苷含量[J].中国实验方剂学杂志,2014,20(6):103-106.
[13]王春秋,李雯霞. RP-HPLC法同时测定金银花中10种化学成分[J].中成药,2015,37(9):1973-1977.
[14]张丽媛,李遇伯,李利新,等. RRLC-Q-TOF/MS分析金银花的化学成分[J].中南药学,2012,10(3):204-208.
[15]李小芩,孙晓红,蔡爽,等.采用UPLC-ESI-MS/MS以及主成分聚类分析研究不同品种金银花的化学成分及其差异[J].药学学报,2009,44(8):895-904.
[16]瞿飞,孙志佳,陈爱茜,等.金银花道地药材的地理标志保护研究[J].山东农业科学,2012,44(2):67-71,76.
[17]张百霞,周凤琴,郭庆梅.金银花中黄酮类化合物的研究进展[J].中国实验方剂学杂志,2012,18(23):349-352.
[18]杨丽军,王丹丹,张允菲,等.金银花中木犀草苷的研究进展[J].中国医药导报,2017,14(14):58-61.
[19]范蕾,蓝云龙,余乐,等.不同产地金银花中绿原酸及木犀草苷的含量测定[J].中华中医药学刊,2013,31(1):172-175.
[20]赵君峰,张彬,张红梅,等.不同产地金银花中绿原酸及木犀草苷的快速检测[J].安徽农业科学,2015,43(15):61-62,65.