牛冠状病毒灭活疫苗的免疫效力试验
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摘要
为了评价制备的牛冠状病毒灭活疫苗的免疫效力,试验选用分离鉴定出来的牛冠状病毒(BCV-YC分离株)为制苗种毒,接种HRT-18细胞进行增殖,收集上清液进行超速离心浓缩,最后加入甲醛灭活制成油乳剂灭活疫苗,分别接种小鼠和妊娠母牛。结果表明,疫苗不仅使小鼠具有一定的保护力,而且使妊娠母牛也能产生较高的抗体水平,犊牛攻毒试验证明,产生的母源抗体能使初生犊牛抵抗牛冠状病毒的攻击。说明制备的牛冠状病毒灭活疫苗对抵抗牛冠状病毒感染具有良好的保护性。
To evaluate the immunological efficacy of the prepared bovine coronavirus inactivated vaccine,the isolated bovine coronavirus(BCV-YC isolate) was selected for seedling toxicity, and inoculated with HRT-18 cells for proliferation;The supernatant was collected for speeding.The cells were concentrated by centrifugation, and finally inactivated by formaldehyde to prepare an oil emulsion inactivated vaccine, and then inoculate the mouse and pregnant cows.The results showed that the vaccine not only give mice a certain protective force, but also produced higher antibody levels in pregnant cows. The challenge test proved that the maternal antibody produced could protect the newborn calves against bovine coronavirus.
To evaluate the immunological efficacy of the prepared bovine coronavirus inactivated vaccine,the isolated bovine coronavirus(BCV-YC isolate) was selected for seedling toxicity, and inoculated with HRT-18 cells for proliferation;The supernatant was collected for speeding.The cells were concentrated by centrifugation, and finally inactivated by formaldehyde to prepare an oil emulsion inactivated vaccine, and then inoculate the mouse and pregnant cows.The results showed that the vaccine not only give mice a certain protective force, but also produced higher antibody levels in pregnant cows. The challenge test proved that the maternal antibody produced could protect the newborn calves against bovine coronavirus.
引文
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[12]刘振格,侯喜林,余丽芸,等.牛冠状病毒DQ株感染BALB/c小鼠的敏感性研究[J].中国预防兽医学报,2014,36(01):15-18.
[13]Besser T E,Gay C C,Mc Guire T C,et al.Passive immunity to bovine rotavirus infection associated with transfer of serum antibody into the intestinal lumen[J].J Virol,1988,62(7):2 238-2 242.
[2]孙留霞,朱平军,李文华,等.牛冠状病毒检测方法的研究进展[J].上海畜牧兽医通讯,2014(5):20-22.
[3]Waltner-Toews D,Martin S W,Meek A H,et al.A field trial to evaluate the efficacy of a combined rotavirus-coronavirus/Escherichia coli vaccine in dairy cattle[J].Can J Comp Med,1985,49(1):1-9.
[4]张俭伟,杨少华,王长法,等.被动免疫预防犊牛轮状病毒感染的研究[J].中国人兽共患病学报,2007,23(6):569-571+579.
[5]Keizo T,Yuichi M,Yukio S.Field study of bovine coronavirus vaccine enriched with hemagglutinating antigen for winter dysentery in dairy cows[J].Can J Vet Res,2002,66(4):278-281.
[6]张礼德,王葳.牛冠状病毒的培养增殖及含毒滴度检测新进展[J].国外兽医学畜禽疾病,1992,13(1):1-5.
[7]中华人民共和国农业部颁.中华人民共和国兽用生物制品规程(1992年版)[S].1992.
[8]韩文榆,何昭阳,刘玉斌.病原细菌检验技术[M].长春:吉林科学技术出版社,1992.
[9]刘合义,孙留霞,王进轶,等.牛冠状病毒重组N蛋白间接ELISA检测方法的建立[J].中国预防兽医学报,2009,31(8):618-622.
[10]刘合义,孙留霞,王进轶,等.牛冠状病毒RT-PCR检测方法的建立[J].中国兽医杂志,2009,45(2):25-27.
[11]谢敏康等编译.俄研制出防制新生犊牛腹泻的四联灭活苗[J].畜牧科技信息.2000,12:18-20.
[12]刘振格,侯喜林,余丽芸,等.牛冠状病毒DQ株感染BALB/c小鼠的敏感性研究[J].中国预防兽医学报,2014,36(01):15-18.
[13]Besser T E,Gay C C,Mc Guire T C,et al.Passive immunity to bovine rotavirus infection associated with transfer of serum antibody into the intestinal lumen[J].J Virol,1988,62(7):2 238-2 242.