饲料和饲料添加剂中蜡样芽孢杆菌分离鉴定及其呕吐毒素基因检测
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摘要
为了掌握饲料及饲料添加剂中蜡样芽孢杆菌分布及其呕吐毒素基因携带情况,从规模化饲料生产企业采集饲料及饲料添加剂样品246批。通过细菌分离、纯化、PCR检测和BD PhoenixTM-100全自动微生物鉴定系统鉴定,共分离蜡样芽孢杆菌63株,分离率为25.6%,从混合型饲料添加剂中检出4株含芽孢杆菌呕吐毒素基因,携带率为13.3%。试验结果表明,饲料和饲料添加剂中存在蜡样芽孢杆菌,尤其是含呕吐毒素蜡样芽孢杆菌污染风险。
In order to grasp the distribution of Bacillus cereus and the carrying status of cereulide gene in feed and feed additives, 246 batches of feed and feed additive samples were collected from large-scale feed production enterprises.Bacillus cereus were isolated and identified by PCR and BD PhoenixTM-100 Compact automatic microbial analysis system. A total of 63 strains of Bacillus cereus were isolated with a separation rate of 25.6%, 4 strains carrying cereulide genes were detected from the mixed feed additives, and the carrying rate was 13.3%. There is a risk of contamination by Bacillus cereus in feed and feed additives, especially by the Bacillus cereus carrying cereulide genes.
In order to grasp the distribution of Bacillus cereus and the carrying status of cereulide gene in feed and feed additives, 246 batches of feed and feed additive samples were collected from large-scale feed production enterprises.Bacillus cereus were isolated and identified by PCR and BD PhoenixTM-100 Compact automatic microbial analysis system. A total of 63 strains of Bacillus cereus were isolated with a separation rate of 25.6%, 4 strains carrying cereulide genes were detected from the mixed feed additives, and the carrying rate was 13.3%. There is a risk of contamination by Bacillus cereus in feed and feed additives, especially by the Bacillus cereus carrying cereulide genes.
引文
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[2]王冬梅,耿晓娜,赵宝华.饲用微生态制剂的应用研究进展[J].畜牧与饲料科学,2010,3(2):54-56.
[3]秦艳,李卫芬,余东游.蜡样芽孢杆菌发酵条件的优化[J].饲料工业,2008,29(2):34-37.
[4]Berthold-pluta A,PLUTA A,Garbowska M.The effect of selected factors on the survival of Bacillus cereus in the human gastrointestinal tract[J].Microb.Pathog.,2015(82):7-14.
[5]王君.全国食品中蜡样芽孢杆菌的污染分布规律及遗传多样性研究[D].广州:广东工业大学,2013.
[6]Chon J W,Kim J H,Lee S J,et al.Toxin profile,antibiotic resistance,and phenotypic and molecular characterization of Bacillus cereus in Sunsik[J].Food Microbiology,2012,32(1):217-222.
[7]张翼.婴幼儿食品中呕吐型蜡样芽孢杆菌的鉴别与多位点序列分析[D].武汉:武汉轻工大学,2015.
[8]Bottone E J.Bacillus cereus,a volatile human pathogen[J].Clin.Microbiol.Rev.,2010,23(2):382-398.
[9]Lim J S,Kim M R,Kim W,et al.Detection and differentiation of nonemetic and emetic Bacillus cereus strains in food by real-time PCR[J].J.Korean Soc.Appl.Biol.Chem.,2011,54(1):105-111.
[10]崔一芳.蜡样芽孢杆菌毒素特征及耐药基因tet(45)的可移动性研究[D].北京:中国农业大学,2016.