洋葱醇提物总多酚含量及其抗氧化活性研究
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摘要
目的建立洋葱醇提物总多酚含量测定方法和评价洋葱醇提物体外抗氧化活性。方法以没食子酸为标准品,采用福林酚法测定洋葱醇提物总多酚含量,采用2,2'-联氮双(3-乙基苯并噻唑啉-6-磺酸)二铵盐(ABTS)和1,1-二苯基-2-三硝基苯肼(DPPH)法评价洋葱醇提物的抗氧化活性。结果洋葱总多酚含量测定的最佳显色条件为显色温度60℃,显色时间60 min,福林酚体积0. 6 m L,10%碳酸钠体积0. 75 m L;没食子酸质量浓度在1. 2～5. 0 mg/L(R2=0. 999 5)范围内与吸光度值线性关系良好,平均回收率为113. 71%,RSD为3. 50%(n=6);洋葱总多酚平均含量为29. 908 8 mg/g,RSD为5. 169 4%(n=6);洋葱醇提物对ABTS自由基的半数抑制浓度(IC50)值为(187. 7±0. 08) mg/L,而阳性对照芦丁对ABTS和DPPH自由基的IC50值为(12. 87±0. 21) mg/L和(10. 29±0. 17)mg/L。结论该方法操作简便、灵敏度高且准确可靠,适合用于测定洋葱总多酚的含量。
Objective To establish a method for the determination of total polyphenol content( TPC) of the ethanol extract from Allium cepa and to evaluate the antioxidant activity in vitro of the ethanol extract from Allium cepa. Methods Gallic acid was used as the standard. The TPC of the ethanol extract from Allium cepa was determined by the Folin-Ciocalteu method. The antioxidant activity of the ethanolic extract from Allium cepa was evaluated by using ABTS and PPPH methods. Results The optimum conditions for the determination of TPC of Allium cepa were as follows: the chromogenic temperature was 60 ℃,the chromogenic time was 60 min,the volume of folin-ciocalteu was 0. 6 m L,and the volume of 10 % sodium carbonate solution was 0. 75 m L. Gallic acid showed good linearity in the range of 1. 2-5. 0 mg/L( R2 = 0. 999 5),the average recovery rate was 113. 71% with RSD of 3. 50%( n = 6). The average TPC of Allium cepa was 29. 908 8 mg/g with RSD of 5. 169 4%( n = 6). The IC50 value of ethanolic extract from Allium cepa against ABTS free radical was( 187. 7 ± 0. 08) mg/L,while the IC50 values of positive control rutin against ABTS and DPPH free radicals were( 12. 87 ± 0. 21) mg/L and( 10. 29 ± 0. 17) mg/L. Conclusion The method is simple,sensitive,accurate and reliable,which is suitable for the determination of TPC of Allium cepa.
Objective To establish a method for the determination of total polyphenol content( TPC) of the ethanol extract from Allium cepa and to evaluate the antioxidant activity in vitro of the ethanol extract from Allium cepa. Methods Gallic acid was used as the standard. The TPC of the ethanol extract from Allium cepa was determined by the Folin-Ciocalteu method. The antioxidant activity of the ethanolic extract from Allium cepa was evaluated by using ABTS and PPPH methods. Results The optimum conditions for the determination of TPC of Allium cepa were as follows: the chromogenic temperature was 60 ℃,the chromogenic time was 60 min,the volume of folin-ciocalteu was 0. 6 m L,and the volume of 10 % sodium carbonate solution was 0. 75 m L. Gallic acid showed good linearity in the range of 1. 2-5. 0 mg/L( R2 = 0. 999 5),the average recovery rate was 113. 71% with RSD of 3. 50%( n = 6). The average TPC of Allium cepa was 29. 908 8 mg/g with RSD of 5. 169 4%( n = 6). The IC50 value of ethanolic extract from Allium cepa against ABTS free radical was( 187. 7 ± 0. 08) mg/L,while the IC50 values of positive control rutin against ABTS and DPPH free radicals were( 12. 87 ± 0. 21) mg/L and( 10. 29 ± 0. 17) mg/L. Conclusion The method is simple,sensitive,accurate and reliable,which is suitable for the determination of TPC of Allium cepa.
引文
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[20] YANG L,XIAN D,XIONG X,et al. Proanthocyanidins against oxidative stress:from molecular mechanisms to clinical applications[J]. BioMed Research International,2018(2018):8584136.
[2] MA YL,ZHU DY,THAKUR K,et al. Antioxidant and antibacterial evaluation of polysaccharides sequentially extracted from onion(Allium cepa L.)[J]. International Journal of Biological Macromolecules,2018,111:92-101.
[3] ABDELRAHMAN M,ALI H,EL-SAYED M,et al. Isolation and characterization of Cepa2,a natural alliospiroside A,from shallot(Allium cepa L. Aggregatum group)with anticancer activity[J].Plant Physiology and Biochemistry,2017(116):167-173.
[4] WANG ZD,LI LH,XIA H,et al. Optimisation of steam distillation extraction oil from onion by response surface methodology and its chemical composition[J]. Natural Product research,2017,32(1):112-115.
[5]欧学兰,杨春艳,魏锦秋,等.洋葱精油对ox-LDL诱导人脐静脉内皮细胞损伤的保护作用[J].中国动脉硬化杂志,2018,26(7):698-704.
[6] VILAHUR G,BADIMON L. Antiplatelet properties of natural products[J]. Vascular pharmacology,2013,59(3-4):67-75.
[7] VIDYAVATI HG,MANJUNATHA H,HEMAVATHY J,et al. Hypolipidemic and antioxidant efficacy of dehydrated onion in experimental rats[J]. Journal of Food Science and Technology,2010,47(1):55-60.
[8] MITRA J,SHRIVASTAVA SL,RAO PS. Onion dehydration:A review[J]. Journal of Food Science and Technology,2012,49(3):267-277.
[9] KIM J,KIM JS,PARK E. Cytotoxic and antiinflammatory effects of onion peel extract on lipopolysaccharide stimulated human colon carcinoma cells[J]. Food and Chemicla Toxicology,2013(62):199-204.
[10]尹文清,宋鑫明,陈光英,等.青梅叶中多酚含量测定及抗氧化活性研究[J].食品科技,2009,34(5):283-286.
[11] YANG C,LI F,DU B,et al,Isolation and Characterization of New Phenolic Compounds with Estrogen Biosynthesis-Inhibiting and Antioxidation Activities from Broussonetia papyrifera Leaves[J].PLoS One,2014,9(4):e94198.
[12] TEJADA S,PINYA S,Bibiloni M,et al,Cardioprotective Effects of the Polyphenol Hydroxytyrosol from Olive Oil[J]. Current Drug Targets,2017(18):1477-1486.
[13]彭茹洁,汪佳丹,韩伟.植物多酚提取、分离纯化及其分析方法的研究进展[J].制药装备,2016,22(5):21-29.
[14] YANG CY,HA W,LIN Y,et al. Polyphenols Isolated from Xanthoceras sorbifolia husks and their anti-tumor and radicalscavenging activities[J]. Molecules,2016,21(12):1694.
[15]陈继英,郭嘉林,张存彦,等.茶多酚的研究进展[J].中草药,2004,35(10):11-13.
[16] SIRACUSA L,AVOLA G,PATANC,et al. Re-evaluation of traditional Mediterranean foods. The local landraces of'Cipolla di Giarratana'(Allium cepa L.)and long-storage tomato(Lycopersiconesculentum L.):quality traits and polyphenol content[J]. Journal of the Science of Food and Agriculture,2013(93):3512-3519.
[17]唐远谋,焦士蓉,刘佳,等.洋葱总多酚的提取工艺及其酶抑制作用初探[J].中国调味品,2011,36(11):25-29.
[18]陈晨,胡文忠,张瑞东,等.鲜切洋葱贮藏期间多酚成分及抗氧化活性的变化[J].营养学报,2016,38(6):596-600.
[19]赵法及.洋葱和大蒜予防动脉粥样硬化症[J].国外医学:参考资料卫生学分册,1979,1:20-22.
[20] YANG L,XIAN D,XIONG X,et al. Proanthocyanidins against oxidative stress:from molecular mechanisms to clinical applications[J]. BioMed Research International,2018(2018):8584136.