黑骨藤追风活络胶囊对胶原诱导性关节炎大鼠IL-1β、IFN-γ、IL-15的影响
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摘要
目的观察黑骨藤追风活络胶囊对胶原诱导性关节炎(CIA)大鼠关节炎症的缓解作用及对血清白细胞介素-1β(IL-1β)、干扰素-γ(IFN-γ)、白细胞介素-15(IL-15)表达水平的影响。方法将40只雄性SD大鼠随机分为正常组、模型组、雷公藤多苷片组和黑骨藤追风活络胶囊组,每组10只,对除正常组外,其余3组建立CIA模型,于初次免疫后10 d开始,给各组大鼠进行相应药物灌胃处理以延缓炎症进展,并每隔7 d评定一次各组大鼠的关节炎指数(AI),给药21 d后处死大鼠检测各组大鼠血清中IL-1β、IFN-γ和IL-15的表达水平。结果正常组大鼠关节正常,而模型组关节红肿较为明显,AI指数评分较高(P<0.001),雷公藤多苷片组和黑骨藤追风活络胶囊组大鼠AI均较模型组有所缓解(P<0.01)。模型组大鼠血清IL-1β、IFN-γ、IL-15水平均较正常组升高(P<0.01),雷公藤多苷片组及黑骨藤追风活络胶囊组大鼠IL-1β、IFN-γ、IL-15水平均显著低于模型组(P<0.05或P<0.01)。结论黑骨藤追风活络胶囊可在一定程度上抑制CIA大鼠促炎因子分泌,缓解关节炎症,可作为临床上治疗类风湿关节炎的辅助用药。
Objective: To investigate the effect of Heiguteng Zhuifeng Huoluo Capsules on the alleviation of collagen-induced arthritis and the influence on the expression of serum IL-1β,IFN-γ and IL-15 in rat model. Methods:40 male SD rats were divided into the normal group(group A),the model group(group B),Tripterygium wilfordii glycosides tablets group(group C) and Heiguteng Zhuifeng Huoluo Capsules group(group D) at random,10 rats in each. CIA model was established in group B,C and D. The rats were given relative drugs 10 days after the first inoculation to delay the progress of inflammation,and the arthritis index(AI) of rats in each group was evaluated every 7 days. After 21 days of drug administration,the rats were sacrificed and the levels of IL-1β,IL-15 and IFN-γ in the serum were measured to observe the effect of the drug on serum cytokines. Results: In the normal group,the joints of the rats were normal,but the joints in the model group were more red and swollen,and the AI index score was higher(P < 0.001). Compared with the model group,the joints and AI in group C and D visibly alleviated(P < 0.01). The levels of serum IL-1β,IFN-γ and IL-15 in the model group dramatically increased,compared with the normal group and there was a significant decline of the levels of these cytokines in group C and D,compared with group B. Conclusion: Heiguteng Zhuifeng Huoluo Capsules can significantly inhibit the secretion of proinflammatory cytokines and reduce the degree of arthritis in CIA rats. It can be used as an auxiliary drug for the clinical treatment of rheumatoid arthritis.
Objective: To investigate the effect of Heiguteng Zhuifeng Huoluo Capsules on the alleviation of collagen-induced arthritis and the influence on the expression of serum IL-1β,IFN-γ and IL-15 in rat model. Methods:40 male SD rats were divided into the normal group(group A),the model group(group B),Tripterygium wilfordii glycosides tablets group(group C) and Heiguteng Zhuifeng Huoluo Capsules group(group D) at random,10 rats in each. CIA model was established in group B,C and D. The rats were given relative drugs 10 days after the first inoculation to delay the progress of inflammation,and the arthritis index(AI) of rats in each group was evaluated every 7 days. After 21 days of drug administration,the rats were sacrificed and the levels of IL-1β,IL-15 and IFN-γ in the serum were measured to observe the effect of the drug on serum cytokines. Results: In the normal group,the joints of the rats were normal,but the joints in the model group were more red and swollen,and the AI index score was higher(P < 0.001). Compared with the model group,the joints and AI in group C and D visibly alleviated(P < 0.01). The levels of serum IL-1β,IFN-γ and IL-15 in the model group dramatically increased,compared with the normal group and there was a significant decline of the levels of these cytokines in group C and D,compared with group B. Conclusion: Heiguteng Zhuifeng Huoluo Capsules can significantly inhibit the secretion of proinflammatory cytokines and reduce the degree of arthritis in CIA rats. It can be used as an auxiliary drug for the clinical treatment of rheumatoid arthritis.
引文
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[11]王梦涛,倪丹红,王晓元,等.艾拉莫德对类风湿关节炎患者CD3+T细胞IFN-γ及细胞因子IL-6调节作用的研究[J].中国地方病防治杂志,2016(12):310-310.
[12]Nogueira E,Gomes AC,Preto A,et al.Folate-targeted nanoparticles for rheumatoid arthritis therapy[J].Nanomedicine Nanotechnology Biology&Medicine,2016,12(4):1113.
[13]王梁谦,唐杨,李修洋,等.血清中炎性细胞因子在类风湿关节炎患者中的检测及临床意义[J].海南医学院学报,2016,(8):762-764.
[14]Vermeire K,Heremans H,Vandeputte M,et al.Accelerated collagen-induced arthritis in IFN-gamma receptor-deficient mice[J].Journal of Immunology,1997,158(11):5507-5513.
[15]Katrina B,Eames HL,Miriam W,et al.IFN-λre solves inflammation via suppression of neutrophil infiltration and IL-1βproduction[J].Journal of Experimental Medicine,2015,212(6):845-853.
[16]Mcinnes IB,Liew FY.Interleukin 15:a proinflammatory role in rheumatoid arthritis synovitis[J].Immunology Today,1998,19(2):75.
[17]Ernestam S,Klint EA,Catrina AI,et al.Synovial expression of IL-15 in rheumatoid arthritis is not influenced by blockade of tumour necrosis factor[J].Arthritis Research&Therapy,2005,8(1):1-8.
[2]袁涛,鲁俊山,马勇.藤类中药在骨科疾病中的应用现状[J].辽宁中医药大学学报,2012(9):118-120.
[3]徐强,戈海青,王晓军,等.藤类药物在风湿病中的应用现状[J].新中医,2013,45(10):124-127.
[4]李慧敏,赵喜玲,李小云,等.黑骨藤抗类风湿性关节炎的有效成分研究[J].中药材,2016,39(3):649-651.
[5]侯玉婷,黎霞,张宏,等.复方黑骨藤抗类风湿关节炎作用及机制的研究[J].四川师范大学学报:自然科学版,2016,39(1):123-127.
[6]冉然,侯玉婷,黎霞.复方黑骨藤对佐剂性关节炎大鼠IL-4、IFN-γ及TGF-β1表达的影响[J].中国免疫学杂志,2017,33(8):1164-1169.
[7]陆墨姼,张宏,刘刚,等.复方黑骨藤提取物对佐剂性关节炎大鼠滑膜组织中TNF-α、MMP-9和TIMP-1的影响[J].时珍国医国药,2011,22(11):2593-2595.
[8]骆镜妃,胡宗科,邓康,等.黑骨藤追风活络胶囊抗类风湿性关节炎的药理作用及机制[J].中南医学科学杂志,2016,44(2):140-142.
[9]顾夙,徐守宇.白花蛇舌草对胶原诱导性关节炎大鼠的保护作用[J].中国生化药物杂志,2014,34(9):64-67.
[10]王和鸣,葛继荣,陈治英.黑骨藤追风活络胶囊治疗痹病的临床研究[J].中国中医骨伤科杂志,1999,7(2):12-14.
[11]王梦涛,倪丹红,王晓元,等.艾拉莫德对类风湿关节炎患者CD3+T细胞IFN-γ及细胞因子IL-6调节作用的研究[J].中国地方病防治杂志,2016(12):310-310.
[12]Nogueira E,Gomes AC,Preto A,et al.Folate-targeted nanoparticles for rheumatoid arthritis therapy[J].Nanomedicine Nanotechnology Biology&Medicine,2016,12(4):1113.
[13]王梁谦,唐杨,李修洋,等.血清中炎性细胞因子在类风湿关节炎患者中的检测及临床意义[J].海南医学院学报,2016,(8):762-764.
[14]Vermeire K,Heremans H,Vandeputte M,et al.Accelerated collagen-induced arthritis in IFN-gamma receptor-deficient mice[J].Journal of Immunology,1997,158(11):5507-5513.
[15]Katrina B,Eames HL,Miriam W,et al.IFN-λre solves inflammation via suppression of neutrophil infiltration and IL-1βproduction[J].Journal of Experimental Medicine,2015,212(6):845-853.
[16]Mcinnes IB,Liew FY.Interleukin 15:a proinflammatory role in rheumatoid arthritis synovitis[J].Immunology Today,1998,19(2):75.
[17]Ernestam S,Klint EA,Catrina AI,et al.Synovial expression of IL-15 in rheumatoid arthritis is not influenced by blockade of tumour necrosis factor[J].Arthritis Research&Therapy,2005,8(1):1-8.