氯氰菊酯对老年鼠脑皮层及血清中5-HT、DA、NE的影响
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摘要
目的:探讨氯氰菊酯染毒对老年C57BL/6小鼠脑皮层及血清中单胺类神经递质5-羟色胺(5-HT)、多巴胺(DA)和去甲肾上腺素(NE)的影响。方法:以20只健康老年C57BL/6小鼠为研究对象,雌雄各半,随机分成染毒组及对照组,其中染毒组给予氯氰菊酯100mg/kg(腹腔注射),每日1次,连续4日;对照组给予等体积生理盐水(腹腔注射)。染毒期间每日对小鼠的日常行为及体质量进行观察。染毒结束后,摘眼球取血、断头处死小鼠,分离血清及脑皮层,用ELISA法检测老年C57BL/6小鼠脑皮层及血清中5-HT、DA、NE的含量。结果:对照组C57BL/6小鼠一般状况较好,染毒组则精神萎靡,眼角有分泌物残留,身体有撕咬痕迹。染毒前后老年C57BL/6小鼠体质量变化无统计学差异(P>0.05)。染毒组老年雄小鼠脑皮层及血清中5-HT、DA的含量低于对照组(P<0.05);老年雌小鼠脑皮层及血清中5-HT、DA、NE的含量高于对照组(P<0.05)。结论:氯氰菊酯急性染毒对老年C57BL/6雄小鼠及雌小鼠脑皮层及其血清中5-HT、DA、NE的含量产生一定影响。
Objective:To investigate the acute effects of cypermethrin exposure on serotonin,dopamine and noradrenaline in C57 BL/6 mice cerebral cortex and serum.Methods:10 healthy elderly females and Elderly males C57 BL/6 mice were randomly divided into two groups(namely the treatment group and the control group).C57 BL/6 mice treated with cypermethrin(i.p.doses at 100 mg/kg)or equal volume of normal saline(i.p.)for four days.Daily behaviors and body mass of mice were observed during the experiment.At the end of the experiment,blood was taken from the eyeball and sacrificed to the mouse with head broken.Serum and cortex was separated.The contents of serotonin(5-HT),dopamine(DA)and noradrenaline(NE)in the cortex and serum of senile mice were measured by ELISA.Results:The old mice in the control group were generally in good condition.The mice in the treated group showed depression,there is secretion in the corner of the eye and signs of tearing.There was no statistical difference in the body mass.The content of 5-HT and DA in the cortex and serum of the treated male mice was lower than that of the control group(P<0.05).The content of 5-HT and DA in the cortex and serum of the treated female mice was higher than that of the control group(P<0.05).Conclusion:Acute toxicity of cypermethrin affected the contents of 5-HT,DA and NE in the cortex and serum of C57 BL/6 mice.
Objective:To investigate the acute effects of cypermethrin exposure on serotonin,dopamine and noradrenaline in C57 BL/6 mice cerebral cortex and serum.Methods:10 healthy elderly females and Elderly males C57 BL/6 mice were randomly divided into two groups(namely the treatment group and the control group).C57 BL/6 mice treated with cypermethrin(i.p.doses at 100 mg/kg)or equal volume of normal saline(i.p.)for four days.Daily behaviors and body mass of mice were observed during the experiment.At the end of the experiment,blood was taken from the eyeball and sacrificed to the mouse with head broken.Serum and cortex was separated.The contents of serotonin(5-HT),dopamine(DA)and noradrenaline(NE)in the cortex and serum of senile mice were measured by ELISA.Results:The old mice in the control group were generally in good condition.The mice in the treated group showed depression,there is secretion in the corner of the eye and signs of tearing.There was no statistical difference in the body mass.The content of 5-HT and DA in the cortex and serum of the treated male mice was lower than that of the control group(P<0.05).The content of 5-HT and DA in the cortex and serum of the treated female mice was higher than that of the control group(P<0.05).Conclusion:Acute toxicity of cypermethrin affected the contents of 5-HT,DA and NE in the cortex and serum of C57 BL/6 mice.
引文
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[13]Aldridge J E,Meyer A,Seidler F J,et al.Alterations in central nervous system serotonergic and dopaminergic synaptic activity in adulthood after prenatal or neonatal chlorpyrifos exposure[J].Environ Health Persp,2005,113(8):1027-1031.
[14]Dam K,Seidler F J,Slotkin T A.Chlorpyrifos exposure during a critical neonatal period elicits gender-selective deficits in the development of coordination skills and locomotor activity[J].Brain Res Develop,2000,121(2):179-187.
[2]汪霞,郜兴利,何炳楠,等.拟除虫菊酯类农药的免疫毒性研究进展[J].农药学学报,2017,19(1):1-8.
[3]杜洁,张怡,洪盼盼,等.拟除虫菊酯对斑马鱼氧化代谢相关基因表达影响[J].浙江工业大学学报,2016,44(3):334-339.
[4]Agrawal S,Dixit A,Singh A,et al.Cyclosporine a and MnTMPyP alleviateα-Synuclein expression and aggregation in Cypermethrin-induced parkinsonism[J].Molecular Neurobiology,2015,52(3):1619-1628.
[5]陈鑫.拟除虫菊酯类农药与重金属的复合毒性对高毒沉积物毒性评价的影响及机制研究[D].广州:中国科学院研究生院(广州地球化学研究所),2016.
[6]姚新民,邬春华,吴强恩,等.亚慢性乐果染毒对大鼠脑组织及血清中单胺类神经递质的影响[J].环境与职业医学,2008,25(2):140-143.
[7]杨明,廖八根,苏燕敏.单胺类中枢神经递质与运动能力的研究进展[J].九江医学,2009,24(4):77-79.
[8]Karen D J,Li W,Harp P R,et al.Striatal dopaminergic pathways as a target for the insecticides permethrin and chlorpyrifos[J].Neurotoxicology,2001,22(6):811-817.
[9]刘恭平,石年,梁骏华,等.拟除虫菊酯对大鼠脑黑质纹状体系统多巴胺及其代谢产物的影响[J].中华劳动卫生职业病杂志,2004,22(5):54-56.
[10]Martinez-larra珘naga M R,AnadóN A,MartíNez M A,et al.5-HT loss in rat brain by type II pyrethroid insecticides[J].Toxicol Ind Health,2003,19(7/10):147-155.
[11]Hossain M M,Suzuki T,Richardson J R,et al.Acute effects of pyrethroids on serotonin release in the striatum of awake rats:an in vivo microdialysis study[J].J Biochen Mol Toxic,2013,27(2):150-156.
[12]Tripathi P,Singh A,Agrawal S,et al.Cypermethrin alters the status of oxidative stress in the peripheral blood:Relevance to Parkinsonism[J].J Physiol Biochem,2014,70(4):915-924.
[13]Aldridge J E,Meyer A,Seidler F J,et al.Alterations in central nervous system serotonergic and dopaminergic synaptic activity in adulthood after prenatal or neonatal chlorpyrifos exposure[J].Environ Health Persp,2005,113(8):1027-1031.
[14]Dam K,Seidler F J,Slotkin T A.Chlorpyrifos exposure during a critical neonatal period elicits gender-selective deficits in the development of coordination skills and locomotor activity[J].Brain Res Develop,2000,121(2):179-187.