‘凤丹’牡丹叶轴多糖超声辅助提取工艺优化及抗氧化性研究
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摘要
为优化超声辅助提取‘凤丹’牡丹叶轴多糖的提取工艺,并测定其多糖提取液的抗氧化性,在单因素试验的基础上,以多糖得率为指标,通过L9(34)正交试验优化多糖提取工艺参数,并通过‘凤丹’牡丹叶轴多糖提取液对2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐[2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate),ABTS]自由基和1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基的清除率来评价其抗氧化能力。结果表明,最佳提取工艺参数为超声温度60℃,液料比24∶1(mL/g),超声时间40 min,超声功率200 W,在此条件下多糖得率为(11.328 8±0.525 3)mg/g;‘凤丹’牡丹叶轴多糖清除ABTS+自由基和DPPH自由基的IC50分别为19.316 7μg/g和39.330 0μg/g,‘凤丹’牡丹叶轴多糖具有较强的抗氧化活性,是天然抗氧化剂的良好来源。
The ultrasonic-assisted extraction of polysaccharides from Paeonia suffruticosa 'Feng Dan' rachises was optimized,and the antioxidant properties of the extracted polysaccharides solution were determined.Optimization of extraction parameters for improved extraction efficiency of polysaccharides was done using single factor experiment and an orthogonal array design L9(34). Meanwhile,the antioxidant activities were evaluated by 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate)(ABTS)and 1,1-diphenyl-2-picrylhydrazyl(DPPH). The optimum extraction parameters were determined as follows:the ultrasonic temperature 60 ℃,the ratio of liquid to material 24 ∶ 1(mL/g),ultrasonic time 40 min,ultrasonic power 200 W. Under these conditions,the extraction yield of polysaccharides was(11.328 8±0.525 3)mg/g. The IC50 for ABTS+and DPPH free radical were 19.316 7 μg/g and 39.330 0 μg/g respectively. The extracted polysaccharides solution showed strong antioxidant activities and it could be a kind of good source for natural antioxidants.
The ultrasonic-assisted extraction of polysaccharides from Paeonia suffruticosa 'Feng Dan' rachises was optimized,and the antioxidant properties of the extracted polysaccharides solution were determined.Optimization of extraction parameters for improved extraction efficiency of polysaccharides was done using single factor experiment and an orthogonal array design L9(34). Meanwhile,the antioxidant activities were evaluated by 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate)(ABTS)and 1,1-diphenyl-2-picrylhydrazyl(DPPH). The optimum extraction parameters were determined as follows:the ultrasonic temperature 60 ℃,the ratio of liquid to material 24 ∶ 1(mL/g),ultrasonic time 40 min,ultrasonic power 200 W. Under these conditions,the extraction yield of polysaccharides was(11.328 8±0.525 3)mg/g. The IC50 for ABTS+and DPPH free radical were 19.316 7 μg/g and 39.330 0 μg/g respectively. The extracted polysaccharides solution showed strong antioxidant activities and it could be a kind of good source for natural antioxidants.
引文
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[2]司冰.油用牡丹‘凤丹’的授粉特性研究[D].杨凌:西北农林科技大学,2016:2
[3]王新,成仿云,钟原,等.凤丹牡丹鳞芽离体培养与快繁技术[J].林业科学,2016,52(5):101-110
[4]付磊.‘凤丹’牡丹花色素组成成分及抗氧化能力分析[D].杨凌:西北农林科技大学,2015:1-2
[5]王洪政,李媛媛,刘伟,等.响应面法优化牡丹果荚多糖提取工艺及其抗氧化活性评估[J].植物研究,2015,35(1):127-132
[6]Yi Wen Tao,Geng Yuan Tian.Studies on the Physicochemical Properties,Structure and Antitumor Activity of Polysaccharide YhPS-1 from the Root of Cordalis yanhusuo Wang[J].Chinese Journal of Chemistry,2006,24(2):235-239
[7]Ge Y,Duan Y,Fang G,et al.Study on biological activities of Physalis alkekengi,var.francheti,polysaccharide[J].Journal of the Science of Food&Agriculture,2009,89(9):1593-1598
[8]姜军.山药多糖的分离纯化及其化学结构的初步研究[D].扬州:扬州大学,2007:2
[9]秦梅颂.超声提取技术在中药中的研究进展[J].安徽农学通报,2010,16(13):54-55,78
[10]李冬梅,杭方学,陆海勤,等.超声辅助提取枸杞多糖的研究进展[J].食品工业科技,2015,36(20):392-395
[11]王如涛,吴绵斌,林建平,等.植物多糖分离提取技术的研究进展[J].中国生物工程杂志,2013,33(7):118-123
[12]宋思圆.黄秋葵花多糖的超声提取及其结构和抗氧化活性研究[D].杭州:浙江大学,2017:15
[13]陈刚,马晓,张帅.“凤丹”牡丹种皮多糖提取工艺的研究[J].食品工业科技,2017,38(1):261-264
[14]郭淼,宋江峰,王传凯.火龙果茎多糖的超高压提取及抗氧化活性研究[J].热带作物学报,2017,38(7):1371-1376
[15]王婉冰,徐子恒,王宏军,等.响应面试验优化海蓬子外种皮水溶性多糖提取工艺及其抗菌活性[J].食品科学,2015,36(14):5-9
[16]孟红亚.蒽酮-硫酸法测定空心莲子草中多糖含量[J].凯里学院学报,2016,34(3):67-69
[17]朱玉昌,焦必宁.ABTS法体外测定果蔬类总抗氧化能力的研究进展[J].食品与发酵工业,2005,36(8):77-80
[18]孙丽萍,王大仟,张智武.11种天然植物提取物对DPPH自由基的清除作用[J].食品科学,2009,30(1):45-47
[19]魏然.圆铃大枣多糖提取、纯化及生物活性研究[D].泰安:山东农业大学,2014:23
[20]郭秀春,杜建南,徐志远,等.超声波法提取脐橙皮中多糖的最佳工艺及其抗氧化性研究[J].河南大学学报(医学版),2017,36(4):251-255
[21]邢增威.红菇多糖的提取及体外抗氧化抗癌性质研究[D].广州:华南理工大学,2013:19
[22]杜丁.黑果枸杞多糖的分离纯化及抗氧化活性研究[D].西安:陕西科技大学,2017:21
[23]Zhao B,Zhang J,Guo X,et al.Microwave-assisted extraction,chemical characterization of polysaccharides from Lilium davidii var.unicolor Salisb and its antioxidant activities evaluation[J].Food Hydrocolloids,2013,31(2):346-356
[24]熊磊,陈慧,胡文兵,等.黄金茶多糖超声提取工艺及体外抗氧化研究[J].江西农业大学学报,2017,39(4):801-809