摘要
为优化超声辅助提取‘凤丹’牡丹叶轴多糖的提取工艺,并测定其多糖提取液的抗氧化性,在单因素试验的基础上,以多糖得率为指标,通过L9(34)正交试验优化多糖提取工艺参数,并通过‘凤丹’牡丹叶轴多糖提取液对2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐[2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate),ABTS]自由基和1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基的清除率来评价其抗氧化能力。结果表明,最佳提取工艺参数为超声温度60℃,液料比24∶1(mL/g),超声时间40 min,超声功率200 W,在此条件下多糖得率为(11.328 8±0.525 3)mg/g;‘凤丹’牡丹叶轴多糖清除ABTS+自由基和DPPH自由基的IC50分别为19.316 7μg/g和39.330 0μg/g,‘凤丹’牡丹叶轴多糖具有较强的抗氧化活性,是天然抗氧化剂的良好来源。
The ultrasonic-assisted extraction of polysaccharides from Paeonia suffruticosa 'Feng Dan' rachises was optimized,and the antioxidant properties of the extracted polysaccharides solution were determined.Optimization of extraction parameters for improved extraction efficiency of polysaccharides was done using single factor experiment and an orthogonal array design L9(34). Meanwhile,the antioxidant activities were evaluated by 2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate)(ABTS)and 1,1-diphenyl-2-picrylhydrazyl(DPPH). The optimum extraction parameters were determined as follows:the ultrasonic temperature 60 ℃,the ratio of liquid to material 24 ∶ 1(mL/g),ultrasonic time 40 min,ultrasonic power 200 W. Under these conditions,the extraction yield of polysaccharides was(11.328 8±0.525 3)mg/g. The IC50 for ABTS+and DPPH free radical were 19.316 7 μg/g and 39.330 0 μg/g respectively. The extracted polysaccharides solution showed strong antioxidant activities and it could be a kind of good source for natural antioxidants.
引文
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