Effects of Different Storage Conditions on Physiological and Biochemical Characteristics of Polygala tenuifolia Willd. Seeds
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摘要
[Objectives] Taking Polygala tenuifolia Willd. seeds as experimental material, the changes of physiological and biochemical characteristics of seeds under different storage temperatures and seed moisture contents were studied. [Methods] The seeds of P. tenuifolia Willd. with moisture contents of 3%, 5% and 15% were stored at-10 ℃, 0 ℃, 20 ℃ and 36 ℃, respectively, and the storage time was one year. The physiological and biochemical characteristics of the seeds, such as conductivity, SOD activity, POD activity, and soluble sugar content were studied.[Results] After one-year storage, as the storage temperature and seed moisture content increased, the relative conductivity, MDA content and soluble sugar content of P. tenuifolia Willd. seeds increased, while the SOD activity, POD activity, and protein content declined. This phenomenon was most obvious in the P. tenuifolia Willd. seeds with moisture content of 15%. [Conclusions] This study will provide reference for seed storage of medicinal plants.
[Objectives] Taking Polygala tenuifolia Willd. seeds as experimental material, the changes of physiological and biochemical characteristics of seeds under different storage temperatures and seed moisture contents were studied. [Methods] The seeds of P. tenuifolia Willd. with moisture contents of 3%, 5% and 15% were stored at-10 ℃, 0 ℃, 20 ℃ and 36 ℃, respectively, and the storage time was one year. The physiological and biochemical characteristics of the seeds, such as conductivity, SOD activity, POD activity, and soluble sugar content were studied.[Results] After one-year storage, as the storage temperature and seed moisture content increased, the relative conductivity, MDA content and soluble sugar content of P. tenuifolia Willd. seeds increased, while the SOD activity, POD activity, and protein content declined. This phenomenon was most obvious in the P. tenuifolia Willd. seeds with moisture content of 15%. [Conclusions] This study will provide reference for seed storage of medicinal plants.
[Objectives] Taking Polygala tenuifolia Willd. seeds as experimental material, the changes of physiological and biochemical characteristics of seeds under different storage temperatures and seed moisture contents were studied. [Methods] The seeds of P. tenuifolia Willd. with moisture contents of 3%, 5% and 15% were stored at-10 ℃, 0 ℃, 20 ℃ and 36 ℃, respectively, and the storage time was one year. The physiological and biochemical characteristics of the seeds, such as conductivity, SOD activity, POD activity, and soluble sugar content were studied.[Results] After one-year storage, as the storage temperature and seed moisture content increased, the relative conductivity, MDA content and soluble sugar content of P. tenuifolia Willd. seeds increased, while the SOD activity, POD activity, and protein content declined. This phenomenon was most obvious in the P. tenuifolia Willd. seeds with moisture content of 15%. [Conclusions] This study will provide reference for seed storage of medicinal plants.
引文
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[12] LI YP,CHENG QX,XI PZ,et al.Effects of water content on storage physiology of the seed of Polygonatum sibiricum Red[J].Seed,2016,35(5):18-22,26.(in Chinese).
[2] GHISLAIN M,SPOONER DM,RODRIGUEZ F,et al.Selection of highly informative and user-friendly microsatellites(SSRs) for genotyping of cultivated potato[J].Theoretical and Applied Genetics,2004,108(5):881-890.
[3] ZHANG X,NIE G,HUANG LK,et al.Effect of different cold storage periods on Dactylis glomerata seed vigor[J].Pratacultural Science,2018,35(10):2450-2456.(in Chinese).
[4] LIN C,SHEN HQ,GUAN YJ,et al.Changes of physiological,biochemistry and gene expression related to ABA and GA3 in hybrid rice seeds stored at different moisture contents and packing methods[J].Plant Physiology Communications,2017,53(6):1077-1086.(in Chinese).
[5] LU WJ,LI YS.Plant physiology experiment course[M].Beijing:China Forestry Publishing House,2012.(in Chinese).
[6] HAN YQ,JIN HQ,JIA ZY,et al.Effects of seed artificial accelerated aging on physiological and biochemical characteristics of Elymus sibiricus[J].Journal of Inner Mongolia Agricultural University(Natural Science Edition),2017,38(5):22-29.(in Chinese).
[7] LI QL.Study on the characteristics and sowing technology of seed germination of Prunella vugaris L.at different storage time[D].Zhengzhou:Henan Agricultural University,2017.(in Chinese).
[8] LIAO JM,QIN LN,PENG H,et al.Effect of different temperature on seed germination and anti-oxidant enzyme activity of Meconopsis integrifolia[J].Guizhou Agricultural Sciences,2016,44(6):14-17.(in Chinese).
[9] YANG C,LI WS,HUANG D,et al.Effect of storage temperature on vigor of sweet clover seed under different moisture content conditions[J].Acta Agrestia Sinica,2016,24(2):251-257.(in Chinese).
[10] WANG YQ,ZHAO Y,AN PK,et al.Physiological and biochemical changes of angelica seeds during storage with different moisture contents[J].China Journal of Chinese Materia Medica,2012,37(2):181-185.(in Chinese).
[11] BO LP,WU X,JIANG FL,et al.Seed vigor and antioxidant characteristics of non-heading Chinese cabbage seeds during artificial aging[J].Acta Botanica Boreali-Occidentalia Sinica,2011,31(4):724-730.(in Chinese).
[12] LI YP,CHENG QX,XI PZ,et al.Effects of water content on storage physiology of the seed of Polygonatum sibiricum Red[J].Seed,2016,35(5):18-22,26.(in Chinese).