β-catenin与VEGF-C在胆管癌组织中的表达及临床意义
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摘要
目的检测β-连环蛋白(β-catenin)和血管内皮生长因子C(vascular endothelial growth factor-c,VEGFC)在胆管癌组织中的表达情况,探讨其与临床病理特征的关系。方法收治的70例胆管癌患者为观察组,以15例癌旁胆管组织为对照组。采用免疫组织化学(S-P)法检测β-catenin及VEGF-C蛋白的表达,分析其临床意义。结果β-catenin在胆管癌组织中的异常表达率为80. 0%(56/70)高于其在癌旁组织中的异常表达率26. 7%(4/15)(P <0. 05)。相关性分析表明β-catenin在胆管癌组织中的异常表达与胆管癌的淋巴结转移、肝动脉/门静脉受侵密切相关(P <0. 05)。VEGF-C在胆管癌组织中的表达阳性率为78. 6%(55/70)高于癌旁组织中的33. 3%(5/15)(P <0. 05)。VEGF-C在胆管癌组织中的表达与胆管癌的淋巴结转移、肝动脉/门静脉受侵密切相关(P <0. 05)。β-catenin与VEGF-C在胆管癌组织中的阳性表达呈正相关(r=0. 652,P=0. 000)。结论β-catenin与VEGF-C表达可能与胆管癌的发生、发展有关。
Objective To investigae the the expressions ofβ-catenin and vascular endothelial growth factor-C(VEGFC) in cholangiocarcinoma,and to explore their relationship with clinical case characteristics. Methods A total of 70 patients with cholangiocarcinoma who were admitted and treated in our hospital were enrolled as observation group,and 15 cases of adjacent cancer tissues were collected as control group. The expression levels of β-catenin and VEGF-C protein in both groups were detected by immunohistochemistry(SP method) to analyse their cinical significance. Results The abnormal expression rate of β-catenin in observation group was 80. 0%(56/70),which was significantly higher than that(26. 7%,4/15) in control group(P < 0. 05). The abnormal expressions of β-catenin in observation group were significantly positively correlated with hepatic artery and/or portal vein invasion as well as lymph node metastasis(P < 0. 05). The positice expression rate of VEGF-C in observation group was 78. 6%(55/70),which was significantly higher than that(33. 3%,5/15) in control group(P < 0. 05). The positive expressions of VEGF-C in observation group were significantly positively correlated with hepatic artery and/or portal vein invasion and lymph node metastasis(P < 0. 05). Moreover the expression of β-catenin was positively correlated with that of VEGF-C expression in observation group(r = 20. 652,P < 0. 01). Conclusion The expressions of β-catenin and VEGF-C are closly associated with the pathogenesis and development of cholangiocarcinoma.
Objective To investigae the the expressions ofβ-catenin and vascular endothelial growth factor-C(VEGFC) in cholangiocarcinoma,and to explore their relationship with clinical case characteristics. Methods A total of 70 patients with cholangiocarcinoma who were admitted and treated in our hospital were enrolled as observation group,and 15 cases of adjacent cancer tissues were collected as control group. The expression levels of β-catenin and VEGF-C protein in both groups were detected by immunohistochemistry(SP method) to analyse their cinical significance. Results The abnormal expression rate of β-catenin in observation group was 80. 0%(56/70),which was significantly higher than that(26. 7%,4/15) in control group(P < 0. 05). The abnormal expressions of β-catenin in observation group were significantly positively correlated with hepatic artery and/or portal vein invasion as well as lymph node metastasis(P < 0. 05). The positice expression rate of VEGF-C in observation group was 78. 6%(55/70),which was significantly higher than that(33. 3%,5/15) in control group(P < 0. 05). The positive expressions of VEGF-C in observation group were significantly positively correlated with hepatic artery and/or portal vein invasion and lymph node metastasis(P < 0. 05). Moreover the expression of β-catenin was positively correlated with that of VEGF-C expression in observation group(r = 20. 652,P < 0. 01). Conclusion The expressions of β-catenin and VEGF-C are closly associated with the pathogenesis and development of cholangiocarcinoma.
引文
1朱金海,张琼,董慧明,等.β-连环素与血管内皮生长因子-C在甲状腺乳头状癌中的表达及其相关性.蚌埠医学院学报,2015,40:587-589.
2 Maruyama K,Ochiai A,Akimoto S,et al. Cytoplasmicβ-catenin accumulation as a predictor of hematogenous metastasis in human colorectal cancer. Oncology,2000,59:302-309.
3 周迪,杨勇,汤朝晖,等.肝门部胆管癌日本诊治体系的历史沿革、现状和瓶颈.中华外科杂志,2019,57:6-9.
4 刘儒彪,刘义,于岚,等.靶向siRNA阻断Wnt/-catenin信号通路对子宫内膜异位症患者在子宫内膜基质细胞VEGF、MMP-9表达的影响.华中科技大学学报(医学版),2016,45:6-12.
5 Chiurillo MA. Role of the Wnt/β-catenin pathway in gastric cancer:An in-depth literature review. World J Exp Med,2015,5:84-102.
6 郎哲,陈刚,王东昌.尼美舒利联合奥沙利铂对人肺癌裸鼠移植瘤生长及淋巴转移的抑制作用.中华肿瘤杂志,2012,34:739-744.
7 刘正人,周水根,聊识君,等.上调WIF-1基因表达对胆管癌细胞侵袭力的影响.广东医学,2016,37(增刊):10-11.
8 王雨艳,黄鑫,王琰琰,等. miRNA-15a通过靶向Wnt3a调控Wnt/β-catenin信号通路抑制卵巢癌的发展和转移.中国肿瘤临床,2018,45:712-716.
9 蔡建鹏,黄力,陈伟,等. Wnt信号通路激活在肝门部胆管癌发生中的作用.中华肝脏外科手术学电子杂志,2015,4:323-326.
10 周晓琳.肝癌组织中VEGF-C及其受体Flt-4的表达及意义.临床与实验病理学杂志,2013,29:340-342.
11 王艳清,杨潇,鲜舒,等. SERPINB3、β-catenin和VEGF-C在宫颈癌中的表达.医学分子生物学杂志,2018,15:82-88.
12 张占东,孔晔,姬社青,等.结直肠癌系膜淋巴结血管内皮生长因子受体3和血管内皮生长因子C表达与淋巴管浸润及淋巴结转移的关系研究.中华胃肠外科杂志,2016,19:708-709.
13 江小梅,彭杰文,杨志锋.血管内皮生长因子-C蛋白及mRNA的表达与胃癌预后的关系.广东医学,2014,35:1549-1551.
14 戚诚,赵晓东,刘三光,等.血管内皮生长因子C和细胞周期蛋白D1在胆管癌组织的表达及其意义.中华实验外科杂志,2016,33:1330-1332.
15 周琎,潘超,杨士勇,等.胰腺癌组织HSP90α、VEGF-C蛋白表达及其临床意义.中华胰腺病杂志,2015,15:129-130.
16 陈满宇,呙帆,黄华俊.血管内皮生长因子C通过调节淋巴内皮细胞促进结肠癌细胞生长的研究.中华实验外科杂志,2015,32:2712-2714.
17 宋秋颖,张增雷,张伟,等. RNAi沉默VEGF-C对A549细胞VEGF家族因子及其受体和下游信号通路的影响.中国老年学杂志,2014,34:4564-4566.
18 孟祥武,陈娟,黄淼,等. VEGF调控Wnt/β-catenin信号通路对少突胶质前体细胞增殖迁移的影响.中国比较医学杂志,2018,28:40-45.
2 Maruyama K,Ochiai A,Akimoto S,et al. Cytoplasmicβ-catenin accumulation as a predictor of hematogenous metastasis in human colorectal cancer. Oncology,2000,59:302-309.
3 周迪,杨勇,汤朝晖,等.肝门部胆管癌日本诊治体系的历史沿革、现状和瓶颈.中华外科杂志,2019,57:6-9.
4 刘儒彪,刘义,于岚,等.靶向siRNA阻断Wnt/-catenin信号通路对子宫内膜异位症患者在子宫内膜基质细胞VEGF、MMP-9表达的影响.华中科技大学学报(医学版),2016,45:6-12.
5 Chiurillo MA. Role of the Wnt/β-catenin pathway in gastric cancer:An in-depth literature review. World J Exp Med,2015,5:84-102.
6 郎哲,陈刚,王东昌.尼美舒利联合奥沙利铂对人肺癌裸鼠移植瘤生长及淋巴转移的抑制作用.中华肿瘤杂志,2012,34:739-744.
7 刘正人,周水根,聊识君,等.上调WIF-1基因表达对胆管癌细胞侵袭力的影响.广东医学,2016,37(增刊):10-11.
8 王雨艳,黄鑫,王琰琰,等. miRNA-15a通过靶向Wnt3a调控Wnt/β-catenin信号通路抑制卵巢癌的发展和转移.中国肿瘤临床,2018,45:712-716.
9 蔡建鹏,黄力,陈伟,等. Wnt信号通路激活在肝门部胆管癌发生中的作用.中华肝脏外科手术学电子杂志,2015,4:323-326.
10 周晓琳.肝癌组织中VEGF-C及其受体Flt-4的表达及意义.临床与实验病理学杂志,2013,29:340-342.
11 王艳清,杨潇,鲜舒,等. SERPINB3、β-catenin和VEGF-C在宫颈癌中的表达.医学分子生物学杂志,2018,15:82-88.
12 张占东,孔晔,姬社青,等.结直肠癌系膜淋巴结血管内皮生长因子受体3和血管内皮生长因子C表达与淋巴管浸润及淋巴结转移的关系研究.中华胃肠外科杂志,2016,19:708-709.
13 江小梅,彭杰文,杨志锋.血管内皮生长因子-C蛋白及mRNA的表达与胃癌预后的关系.广东医学,2014,35:1549-1551.
14 戚诚,赵晓东,刘三光,等.血管内皮生长因子C和细胞周期蛋白D1在胆管癌组织的表达及其意义.中华实验外科杂志,2016,33:1330-1332.
15 周琎,潘超,杨士勇,等.胰腺癌组织HSP90α、VEGF-C蛋白表达及其临床意义.中华胰腺病杂志,2015,15:129-130.
16 陈满宇,呙帆,黄华俊.血管内皮生长因子C通过调节淋巴内皮细胞促进结肠癌细胞生长的研究.中华实验外科杂志,2015,32:2712-2714.
17 宋秋颖,张增雷,张伟,等. RNAi沉默VEGF-C对A549细胞VEGF家族因子及其受体和下游信号通路的影响.中国老年学杂志,2014,34:4564-4566.
18 孟祥武,陈娟,黄淼,等. VEGF调控Wnt/β-catenin信号通路对少突胶质前体细胞增殖迁移的影响.中国比较医学杂志,2018,28:40-45.