基于热带玉米种质的高效诱导自交系筛选
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摘要
分别以热带玉米群体苏湾1号、苏兰3号和QR273/LCH645的F2后代为受体亲本,4份具有不同来源的诱导系作父本,组配规模为20个果穗/组合的杂交后代。根据子粒的胚和胚乳显色差异鉴定出单倍体子粒,评估不同诱导系和母本群体的诱导率。结果表明,苏湾1号呈现出较其改良材料低的诱导率,随着温带血缘的增加,诱导率逐渐增加。针对相同受体材料,不同诱导系的诱导率之间存在显著差异,其中,诱导系1619表现出最高的诱导率(6.28±0.20~7.94±0.85),诱导系1613表现出最低的诱导率(1.93±0.19~3.68±0.05),诱导系1619在诱导热带种质产生单倍体方面具有较高的效率。
Fifty crosses for each pair-parent were constructed, by using tropical maize germplasm of Suwan1,Sulan3, and the F2 progeny of QR273 crossed with LCH645, four induced lines from different resources to be themale parent. Each haploid kernel was identified based on the color performance of the kernel, with purple endo-sperm and white embryo, after pollination. Based on this, the induced rate for each inducer was calculated. The re-sult showed that, lower induced rate was found for Suwan1, and the induced rate was increasing with the increasedintrogression of temperate gene resources. For identical acceptor parent, remarkable difference of induced rate wasfound between different induced lines. Where in 1619 showed the highest value, with the induced rate varied from6.28±0.20 to 7.94±0.85, inducer line of 1613 showed the lowest value, with induced rate varied from 1.93±0.19 to3.68±0.05, which clarified that 1619 is better for the inducing of diploid lines for tropical germplasm.
Fifty crosses for each pair-parent were constructed, by using tropical maize germplasm of Suwan1,Sulan3, and the F2 progeny of QR273 crossed with LCH645, four induced lines from different resources to be themale parent. Each haploid kernel was identified based on the color performance of the kernel, with purple endo-sperm and white embryo, after pollination. Based on this, the induced rate for each inducer was calculated. The re-sult showed that, lower induced rate was found for Suwan1, and the induced rate was increasing with the increasedintrogression of temperate gene resources. For identical acceptor parent, remarkable difference of induced rate wasfound between different induced lines. Where in 1619 showed the highest value, with the induced rate varied from6.28±0.20 to 7.94±0.85, inducer line of 1613 showed the lowest value, with induced rate varied from 1.93±0.19 to3.68±0.05, which clarified that 1619 is better for the inducing of diploid lines for tropical germplasm.
引文
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[3]兰琴英,陈泽辉,王安贵,等.优良玉米自交系QB506、QR273及其改良系的遗传多样性分析[J].西南农业学报,2015,28(3):967-974.Lan Q Y,Chen Z H,Wang A G,et al.Analysis of genetic diversity in elite maizeinbred line QBS06,QR273 and their improved inbred lines,Southwest China Journal of Agricultural Sciences[J].Southwest China Journal of Agricultural Sciences,2015,28(3):967-974.(in Chinese)
[4]胡兴,陈泽辉,郭向阳,等.优良玉米自交系QR273改良系的遗传特性及其改良效果[J].贵州农业科学,2014,42(2):35-38.Hu X,Chen Z H,Guo X Y,et al.Genetic characteristics and effects of improved line of superior maize inbred line QR273[J].Guizhou Agricultural Sciences,2014,42(2):35-38.(in Chinese)
[5]李娟,陈泽辉,祝云芳,等.美国先锋玉米杂交种选系的配合力[J].贵州农业科学,2011,39(9):5-8.Li J,Chen Z H,Zhu Y F,et al.Combining ability of maize inbred lines of pioneer USA hybrids[J].Guizhou Agricultural Sciences,2011,39(9):5-8.(in Chinese)
[6]赵廷明,董树亭,张锁良,等.玉米单倍体育种技术研究与应用进展[J].玉米科学,2007,15(5):60-64.Zhao Y M,Dong S T,Zhang S L,et al.Development in the research and application of technology of haploid breeding in maize[J].Journal of Maize Sciences,2007,15(5):60-64.(in Chinese)
[7]Kermicle J L.Androgenesis conditioned by a mutation in maize[J].Science,1969,166,1422-1424.
[8]Chalyk.Properties of maternal haploid maize plants and potential application to maize breeding[J].Euphytica,1994,79:1-2,13-18.
[9]陈绍江,宋同明.利用高油分的花粉直感效应鉴别玉米单倍体[J].作物学报,2003,29(4):587-590.Chen S J,Song T M.Identification haploid with high oil xenia effect in maize[J].Acta Agronomica Sinica,2003,29(4):587-590.(in Chinese)
[10]李浩川,杨继伟,曲彦志,等.玉米不同基础群体孤雌生殖诱导系诱导能力评价[J].玉米科学,2015,23(1):73-77.Li H C,Yang J W,Qu Y Z,et al.Evaluation for inducing parthenogenesis ability of different inducers from different basic populations in maize[J].Journal of Maize Sciences,2015,23(1):73-77.(in Chinese)
[11]苏月贵,田树云,何雪银,等.Stock6杂交诱导不同热带硬粒基因型玉米研究[J].玉米科学,2011,19(5):6-9.Su Y G,Tian S Y,He X Y,et al.Study on the haploid frequency of different tropical flint maize genotypes induced by Stock 6[J].Journal of Maize Sciences,2011,19(5):6-9.(in Chinese)
[12]Battistelli G M,Von Pinho R G,Justus A,et al.Production and identification of doubled haploids in tropical maize[J].Genet Mol Res.,2013,12:4230-4242.
[13]施标,郑洪建,孙大鹏,等.CIMMYT玉米DH育种研究进展:诱导策略及其应用[J].上海农业学报,2013,29(1):91-94.Shi B,Zheng H J,Sun D P,et al.Advances of maize DH breeding research at CIMMYT:Induction strategy and its application[J].Acta Agriculturae Shanghai,2013,29(1):91-94.(in Chinese)