12种杀虫剂对柑橘大实蝇选择行为的影响及击倒作用
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摘要
柑橘大实蝇是柑橘上的重要害虫。本文分别利用Y型嗅觉仪和饲喂法测定了柑橘大实蝇成虫对12种杀虫剂的选择行为和击倒作用,以期筛选出田间防治柑橘大实蝇的高效农药,并为研发其诱杀剂提供理论依据。结果表明:12种杀虫剂中,吡虫啉、阿维菌素对雌成虫具有极显著的驱避作用;多杀霉素、丁硫克百威、阿维菌素、氟铃脲对雄成虫具有极显著的驱避作用;多杀霉素、毒死蜱、噻嗪酮对雌成虫具有显著的驱避作用;啶虫脒、噻虫嗪、乙酰甲胺磷、高效氯氟氰菊酯和高效氯氰菊酯对柑橘大实蝇雌雄成虫既无驱避作用也无引诱作用。同种药剂对柑橘大实蝇雌雄成虫的击倒时间无显著差异,不同药剂间对柑橘大实蝇成虫的击倒时间有显著差异,其中啶虫脒的击倒时间(KD_(50)<1 min)最短。综合考虑杀虫剂对柑橘大实蝇选择行为的影响及击倒作用,认为啶虫脒更适宜作为柑橘大实蝇诱杀剂中的杀虫剂。
Chinese citrus fly, Bactrocera minax(Enderlein) is a devastating pest that attacks citrus fruits. The olfactory behavioral responses and knockdown action of adults B. minax, on 12 kinds of insecticides commonly used in citrus orchards, was respectively measured with Y-olfactometer and artificial feeding method in the laboratory. The high efficiency pesticides for controlling B. minax in the fields, was screened out, so as to provide theoretical basis for the development and application of their bait-and-kill pesticides. Among 12 kinds of insecticides, imidacloprid and avermectin had highly significant repellent effect to female adults, spinosad, carbosulfan, avermectin and hexaflumuron had highly significant repellent effect to male adults, spinosad, chlorpyrifos and buprofezin had significant repellent effect to female adults. Acetamiprid, thiamethoxam, acephate, lambda-cyhalothrin and beta cypermethrin had no repellent or attractant effect to male and female adults, In the same insecticide, there was no significant difference in the knockout time between male and female adults. There was a significant difference in the knockout time against adults among 12 kinds of insecticides, acetamiprid had the shortest knockdown time(KD_(50)<1 min) against adults. Considering the effects of insecticides on the selection behavior and knockdown action of B. minax, it is considered that acetamiprid is more suitable as an insecticide in the trap of citrus fruit fly.
Chinese citrus fly, Bactrocera minax(Enderlein) is a devastating pest that attacks citrus fruits. The olfactory behavioral responses and knockdown action of adults B. minax, on 12 kinds of insecticides commonly used in citrus orchards, was respectively measured with Y-olfactometer and artificial feeding method in the laboratory. The high efficiency pesticides for controlling B. minax in the fields, was screened out, so as to provide theoretical basis for the development and application of their bait-and-kill pesticides. Among 12 kinds of insecticides, imidacloprid and avermectin had highly significant repellent effect to female adults, spinosad, carbosulfan, avermectin and hexaflumuron had highly significant repellent effect to male adults, spinosad, chlorpyrifos and buprofezin had significant repellent effect to female adults. Acetamiprid, thiamethoxam, acephate, lambda-cyhalothrin and beta cypermethrin had no repellent or attractant effect to male and female adults, In the same insecticide, there was no significant difference in the knockout time between male and female adults. There was a significant difference in the knockout time against adults among 12 kinds of insecticides, acetamiprid had the shortest knockdown time(KD_(50)<1 min) against adults. Considering the effects of insecticides on the selection behavior and knockdown action of B. minax, it is considered that acetamiprid is more suitable as an insecticide in the trap of citrus fruit fly.
引文
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Dorji C, Clarke A, Drew R, et al. Seasonal phenology of Bactrocera minax (Diptera: Tephritidae) in western Bhutan [J]. B. Entomol. Res., 2006, 96 (5): 531-538.
Drew RAI, Dorji C, Romig MC,et al. Attractiveness of various combinations of colors and shapes to females and males of Bactrocera minax (Diptera: Tephritidae) in a commercial mandarin grove in Bhutan [J]. J. Econ. Entomol., 2006, 99 (5): 1651-1656.
Du TH, Hua DK, He ZZ, et al. Behavioral responses of adults Bactrocera minax (Diptera: Tephritidae) to volatile organic components from Castanea (Fagales: Fagaceae) [J]. Journal of Environmental Entomology, 2018, 40 (2): 474-484. [杜田华, 华登科, 何章章, 等. 柑橘大实蝇成虫对板栗挥发物的行为反应 [J]. 环境昆虫学报, 2018, 40 (2): 474-484]
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Gong QT. Studies on the Screening of the Chinese Citrus Fly Food Attractants and Fungicides[D]. Chongqing: Southwest University, 2012. [宫庆涛. 柑橘大实蝇食物引诱剂与防治药剂筛选研究 [D]. 重庆: 西南大学, 2012]
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Hu Z, Gui LY, Luo J,et al. Tolerance of adult Tetranychus cinnabarinus to submersion in water [J]. Chinese Journal of Applied Entomology, 2016, 53 (1): 76-82. [胡智, 桂连友, 罗杰, 等. 朱砂叶螨成螨在水中的耐受力 [J]. 应用昆虫学报, 2016, 53 (1): 76-82]
Huang XQ, Li ZY, Li CR, et al. Wing loading and extra loading capacity of adults of the Chinese citrus fruit fly, Bactrocera (Tetradacus) minax (Diptera: Tephritidae) [J]. Acta Entomologica Sinica, 2012, 55 (5): 606-611. [黄秀琴, 李正跃, 李传仁, 等. 柑橘大实蝇成虫的翅载和额外负载能力 [J]. 昆虫学报, 2012, 55 (5): 606-611]
Li S, Zhao J, Zhang XM, et al. Behavioral responses of Bemisia tabaci MED to 13 plant volatiles [J]. Journal of Plant Protection, 2016, 43 (1): 105-110. [李姝, 赵静, 张晓曼, 等. 烟粉虱MED隐种对13种植物挥发性物质的行为反应 [J]. 植物保护学报, 2016, 43 (1): 105-110]
Li ZY, Du TH, Consolatha C,et al. Study on the optimal trapping period of Bactrocera minax [J]. Journal of Fruit Science, 2018, 35 (11): 1406-1412. [李再园, 杜田华, Consolatha Chambi, 等. 柑橘大实蝇成虫田间诱杀适期探究 [J]. 果树学报, 2018, 35 (11): 1406-1412]
Liu L, Zhou Q, Song AQ, et al. Adult oviposition and larval feeding preference for different citrus varieties in Bactrocera minax (Diptera: Tephritidae) [J]. Acta Entomologica Sinica, 2014, 57 (9): 1037-1044. [刘路, 周琼, 宋傲群, 等. 柑橘大实蝇对不同柑橘品种的产卵偏好和幼虫取食选择 [J]. 昆虫学报, 2014, 57 (9): 1037-1044]
Luo J, Gui LY, Boiteau G,et al. Study on the application of insect harmonic radar in the behavior of Chinese citrus fly [J]. Journal of Environmental Entomology, 2016, 38 (3): 514-521. [罗杰, 桂连友, Gilles Boiteau, 等. 昆虫谐波雷达在柑橘大实蝇行为学上的应用研究 [J]. 环境昆虫学报, 2016, 38 (3): 514-521]
Ma ZQ. Studies on the Relationship Between the Symptoms and Mechanism of Different Kinds of Insecticides [D]. Xian: Northwest A&F University, 2012. [马致卿. 不同类杀虫药剂的致毒症状与作用机理关系研究 [D]. 西安: 西北农林科技大学, 2002]
Morozov VN, Kanev IL. Knockdown of fruit flies by imidacloprid nanoaerosol [J].Environ. Sci. Technol., 2015, 49 (20): 12483-12489.
Pelosi P, Iovinella I, Zhu J,et al. Beyond chemoreception: Diverse tasks of soluble olfactory proteins in insects [J]. Biol. Rev. Camb. Philos. Soc., 2017, 93 (1): 1-17.
Raguso RA, Light DM, Pickersky E. Electroantennogram responses of Hyles lineata (Sphingidae: Lepidoptera) to volatile compounds from Clarkia breweri (Onagraceae) and other moth-pollinated flowers [J]. J. Chem. Ecol., 1996, 22 (10): 1735-1766.
Ran F, Yuan WB, Zhang YD. Application of several attractants to monitor the occurrence period and control period of adult fruit fly ofthe Chinese citrus fly [J]. Zhejiang Citrus, 2011, 28 (2): 29-31. [冉峰, 袁文斌, 张亚东. 应用几种诱剂监测柑橘大实蝇成虫发生期及防治适期初报 [J]. 浙江柑桔, 2011, 28 (2): 29-31]
Shu XL, Xiao ZJ. Occurrence and control of the Chinese citrus fly [J]. Plant Doctor, 2006, 19 (3): 26-26. [舒祥伦, 肖志健. 柑橘大实蝇的发生与防除 [J]. 植物医生, 2006, 19 (3): 26-26]
Tang QY, Feng MG. DPS'Data Processing System for Practical Statistics [M]. Beijing: Science Press, 2002. [唐启义, 冯明光. 实用统计分析及其DPS数据处理系统 [M]. 北京: 科学出版社, 2002]
Vogt RG,Riddiford LM. Pheromone binding and inactivation by moth antennae [J]. Nature, 1981, 293 (5828): 161-163.
Wang FL. Fundamental Study on Adult Control of Bactrocera minax (Enderlein) [D]. Jingzhou: Yangtze University, 2018. [王福莲. 荆州:柑橘大实蝇成虫防控技术基础研究[D]. 长江大学, 2018]
Wang JT, Dong YC, Li ZK, et al. Overview of the use of the sterile insect technique to control the Chinese citrus fruit fly [J]. Chinese Journal of Applied Entomology, 2013, 50 (3): 848-852. [王金涛, 董永成, 李宗锴, 等. 昆虫不育技术防治柑橘大实蝇研究概况 [J]. 应用昆虫学报, 2013, 50 (3): 848-852]
Webster B. The role of olfaction in aphid host location [J].Physiol. Entomol., 2012, 37 (1): 10-18.
Winks RG. The toxicity of phosphine to adults of Tribolium castaneum (Herbst): Phosphine-induced narcosis [J]. J. Stored Prod. Res., 1985, 21 (1): 25-29.
Xiao W, Wu KM, Gong QT, et al. Pheromonal activity of rectum extracts from Chinese citrus fly (Bactrocera minax) [J]. Scientia Agricultura Sinica, 2013, 46 (7): 1501-1508. [肖伟, 武可明, 宫庆涛, 等. 柑橘大实蝇直肠粗提物的信息素功能 [J]. 中国农业科学, 2013, 46 (7): 1501-1508]
Yang HY, Zhou LP. Trapping effect of different attractant formula on the Chinese citrus fly [J]. Agricultural Technology Service, 2011, 28 (1): 48, 51. [杨鸿雁, 周莉萍. 不同诱杀剂配方对柑橘大实蝇的诱杀效果 [J]. 农技服务, 2011, 28 (1): 48, 51]
Zeng ZH, Liu H, Xiao GM, et al. New breakthroughs in control technology of the Chinese citrus fly [J]. Plant Quarantine, 2011, 25 (5): 36-39. [曾卓华, 刘洪, 肖国民, 等. 柑橘大实蝇防控技术的新突破 [J]. 植物检疫, 2011, 25 (5): 36-39]
Zhang GF, Wang FL, LV ZC, et al. Research progress on the biology, ecology and the application of sterile insect technique on Bactrocera minax (Enderlein) [J]. Journal of Biosafety, 2015, 24 (2): 171-176. [张桂芬, 王福莲, 吕志创, 等. 柑橘大实蝇生物、生态学及辐照不育技术最新研究进展 [J]. 生物安全学报, 2015, 24 (2): 171-176]
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Chen SX, Xie YZ. Taxonomic notes on the Chinese citrus fly,Tetradacus citri (Chen) [J]. Acta Entomologica Sinica, 1955, 5 (1): 123-126. [陈世骧, 谢蕴贞. 关于橘大实蝇的学名及其种征 [J]. 昆虫学报, 1995, 1: 123-126]
Chen ZC, Su L, Ge F, et al. Electroantennogram responses of the green leaf bug, Lygus lucorum Meyer Dür (Hemiptera: Miridae), to sex pheromone analogs and plant volatiles [J]. Acta Entomologica Sinica, 2010, 53 (1): 47-54. [陈展册, 苏丽, 戈峰, 等. 绿盲蝽对性信息素类似物和植物挥发物的触角电位反应 [J]. 昆虫学报, 2010, 53 (1): 47-54]
Dong YC, Wang ZJ, Clarke AR,et al. Pupal diapause development and termination is driven by low temperature chilling in Bactrocera minax [J]. J. Pest Sci., 2013, 86 (3): 429-436.
Dorji C, Clarke A, Drew R, et al. Seasonal phenology of Bactrocera minax (Diptera: Tephritidae) in western Bhutan [J]. B. Entomol. Res., 2006, 96 (5): 531-538.
Drew RAI, Dorji C, Romig MC,et al. Attractiveness of various combinations of colors and shapes to females and males of Bactrocera minax (Diptera: Tephritidae) in a commercial mandarin grove in Bhutan [J]. J. Econ. Entomol., 2006, 99 (5): 1651-1656.
Du TH, Hua DK, He ZZ, et al. Behavioral responses of adults Bactrocera minax (Diptera: Tephritidae) to volatile organic components from Castanea (Fagales: Fagaceae) [J]. Journal of Environmental Entomology, 2018, 40 (2): 474-484. [杜田华, 华登科, 何章章, 等. 柑橘大实蝇成虫对板栗挥发物的行为反应 [J]. 环境昆虫学报, 2018, 40 (2): 474-484]
Fan JA. Occurrence and control of the Chinese citrus fly in Sichuan [J]. Plant Quarantine, 2002, 16 (3): 150-152. [范京安. 四川柑桔大实蝇发生与防治 [J]. 植物检疫, 2002, 16 (3): 150-152]
Gong QT. Studies on the Screening of the Chinese Citrus Fly Food Attractants and Fungicides[D]. Chongqing: Southwest University, 2012. [宫庆涛. 柑橘大实蝇食物引诱剂与防治药剂筛选研究 [D]. 重庆: 西南大学, 2012]
He ZZ, Hua DK, Du TH, et al. Feeding behaviour of Bactrocera minax (Diptera: Trypetidae) on male inflorescence of Castanea mollissima (Fagales: Fagaceae) [J]. Acta Entomologica Sinica, 2018, 61 (4): 458-467. [何章章, 华登科, 杜田华, 等. 柑橘大实蝇成虫在中国板栗雄花花序上的取食行为 [J]. 昆虫学报, 2018, 61 (4): 458-467]
Hu Z, Gui LY, Luo J,et al. Tolerance of adult Tetranychus cinnabarinus to submersion in water [J]. Chinese Journal of Applied Entomology, 2016, 53 (1): 76-82. [胡智, 桂连友, 罗杰, 等. 朱砂叶螨成螨在水中的耐受力 [J]. 应用昆虫学报, 2016, 53 (1): 76-82]
Huang XQ, Li ZY, Li CR, et al. Wing loading and extra loading capacity of adults of the Chinese citrus fruit fly, Bactrocera (Tetradacus) minax (Diptera: Tephritidae) [J]. Acta Entomologica Sinica, 2012, 55 (5): 606-611. [黄秀琴, 李正跃, 李传仁, 等. 柑橘大实蝇成虫的翅载和额外负载能力 [J]. 昆虫学报, 2012, 55 (5): 606-611]
Li S, Zhao J, Zhang XM, et al. Behavioral responses of Bemisia tabaci MED to 13 plant volatiles [J]. Journal of Plant Protection, 2016, 43 (1): 105-110. [李姝, 赵静, 张晓曼, 等. 烟粉虱MED隐种对13种植物挥发性物质的行为反应 [J]. 植物保护学报, 2016, 43 (1): 105-110]
Li ZY, Du TH, Consolatha C,et al. Study on the optimal trapping period of Bactrocera minax [J]. Journal of Fruit Science, 2018, 35 (11): 1406-1412. [李再园, 杜田华, Consolatha Chambi, 等. 柑橘大实蝇成虫田间诱杀适期探究 [J]. 果树学报, 2018, 35 (11): 1406-1412]
Liu L, Zhou Q, Song AQ, et al. Adult oviposition and larval feeding preference for different citrus varieties in Bactrocera minax (Diptera: Tephritidae) [J]. Acta Entomologica Sinica, 2014, 57 (9): 1037-1044. [刘路, 周琼, 宋傲群, 等. 柑橘大实蝇对不同柑橘品种的产卵偏好和幼虫取食选择 [J]. 昆虫学报, 2014, 57 (9): 1037-1044]
Luo J, Gui LY, Boiteau G,et al. Study on the application of insect harmonic radar in the behavior of Chinese citrus fly [J]. Journal of Environmental Entomology, 2016, 38 (3): 514-521. [罗杰, 桂连友, Gilles Boiteau, 等. 昆虫谐波雷达在柑橘大实蝇行为学上的应用研究 [J]. 环境昆虫学报, 2016, 38 (3): 514-521]
Ma ZQ. Studies on the Relationship Between the Symptoms and Mechanism of Different Kinds of Insecticides [D]. Xian: Northwest A&F University, 2012. [马致卿. 不同类杀虫药剂的致毒症状与作用机理关系研究 [D]. 西安: 西北农林科技大学, 2002]
Morozov VN, Kanev IL. Knockdown of fruit flies by imidacloprid nanoaerosol [J].Environ. Sci. Technol., 2015, 49 (20): 12483-12489.
Pelosi P, Iovinella I, Zhu J,et al. Beyond chemoreception: Diverse tasks of soluble olfactory proteins in insects [J]. Biol. Rev. Camb. Philos. Soc., 2017, 93 (1): 1-17.
Raguso RA, Light DM, Pickersky E. Electroantennogram responses of Hyles lineata (Sphingidae: Lepidoptera) to volatile compounds from Clarkia breweri (Onagraceae) and other moth-pollinated flowers [J]. J. Chem. Ecol., 1996, 22 (10): 1735-1766.
Ran F, Yuan WB, Zhang YD. Application of several attractants to monitor the occurrence period and control period of adult fruit fly ofthe Chinese citrus fly [J]. Zhejiang Citrus, 2011, 28 (2): 29-31. [冉峰, 袁文斌, 张亚东. 应用几种诱剂监测柑橘大实蝇成虫发生期及防治适期初报 [J]. 浙江柑桔, 2011, 28 (2): 29-31]
Shu XL, Xiao ZJ. Occurrence and control of the Chinese citrus fly [J]. Plant Doctor, 2006, 19 (3): 26-26. [舒祥伦, 肖志健. 柑橘大实蝇的发生与防除 [J]. 植物医生, 2006, 19 (3): 26-26]
Tang QY, Feng MG. DPS'Data Processing System for Practical Statistics [M]. Beijing: Science Press, 2002. [唐启义, 冯明光. 实用统计分析及其DPS数据处理系统 [M]. 北京: 科学出版社, 2002]
Vogt RG,Riddiford LM. Pheromone binding and inactivation by moth antennae [J]. Nature, 1981, 293 (5828): 161-163.
Wang FL. Fundamental Study on Adult Control of Bactrocera minax (Enderlein) [D]. Jingzhou: Yangtze University, 2018. [王福莲. 荆州:柑橘大实蝇成虫防控技术基础研究[D]. 长江大学, 2018]
Wang JT, Dong YC, Li ZK, et al. Overview of the use of the sterile insect technique to control the Chinese citrus fruit fly [J]. Chinese Journal of Applied Entomology, 2013, 50 (3): 848-852. [王金涛, 董永成, 李宗锴, 等. 昆虫不育技术防治柑橘大实蝇研究概况 [J]. 应用昆虫学报, 2013, 50 (3): 848-852]
Webster B. The role of olfaction in aphid host location [J].Physiol. Entomol., 2012, 37 (1): 10-18.
Winks RG. The toxicity of phosphine to adults of Tribolium castaneum (Herbst): Phosphine-induced narcosis [J]. J. Stored Prod. Res., 1985, 21 (1): 25-29.
Xiao W, Wu KM, Gong QT, et al. Pheromonal activity of rectum extracts from Chinese citrus fly (Bactrocera minax) [J]. Scientia Agricultura Sinica, 2013, 46 (7): 1501-1508. [肖伟, 武可明, 宫庆涛, 等. 柑橘大实蝇直肠粗提物的信息素功能 [J]. 中国农业科学, 2013, 46 (7): 1501-1508]
Yang HY, Zhou LP. Trapping effect of different attractant formula on the Chinese citrus fly [J]. Agricultural Technology Service, 2011, 28 (1): 48, 51. [杨鸿雁, 周莉萍. 不同诱杀剂配方对柑橘大实蝇的诱杀效果 [J]. 农技服务, 2011, 28 (1): 48, 51]
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