伤科解毒颗粒的薄层色谱研究
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摘要
目的:建立伤科解毒颗粒的薄层色谱鉴别方法。方法:对伤科解毒颗粒中金银花、延胡索、柴胡等进行薄层色谱研究,对供试品的前处理、展开剂、展开条件以及显色方法等进行研究。结果:伤科解毒颗粒色谱中在与金银花、延胡索、柴胡对照药材相应的位置上显相同颜色荧光斑点,且斑点清晰,分离度较好,阴性对照无干扰,专属性强。结论:该方法简便可行,重现性好,可用于伤科解毒颗粒的质量控制。
Objective To establish a TLC identification method for Shangke Jiedu granule. Methods Thin layer chroma tography was used to study honeysuckle,corydalis tuber and radix bupleuri in Shangke Jiedu granule,and the pretreatment,developing agent,developing conditions and color development methods of the sample were studied. Results In Shangke Jiedu granule chromatography,fluorescent spots of the same color appeared at the corresponding positions of honeysuckle,corydalis tuber and radix bupleuri controlmedicinal materials,and the spots were clear,the separation degree was good,the negative control had no interference,and the specificity was strong. Conclusion The methodis simple,feasible and reproducible,and can be used for the quality controlof shangke Jiedu granules.
Objective To establish a TLC identification method for Shangke Jiedu granule. Methods Thin layer chroma tography was used to study honeysuckle,corydalis tuber and radix bupleuri in Shangke Jiedu granule,and the pretreatment,developing agent,developing conditions and color development methods of the sample were studied. Results In Shangke Jiedu granule chromatography,fluorescent spots of the same color appeared at the corresponding positions of honeysuckle,corydalis tuber and radix bupleuri controlmedicinal materials,and the spots were clear,the separation degree was good,the negative control had no interference,and the specificity was strong. Conclusion The methodis simple,feasible and reproducible,and can be used for the quality controlof shangke Jiedu granules.
引文
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[11]梁毅,高平,张勤.活血止疼合剂的薄层鉴别研究[J].时珍国医国药,2008,19(9):2210-2212.
[12]朱华,茵潇磊,陈勇.绿原酸、隐绿原酸和新绿原酸在中性和碱性PH条件下的降解动力学[J].药学学报,2016,51(1):122-126.
[13]吴宁,胡俊杰,郑国华,等.益母妇宁胶囊的薄层色谱鉴别[J].时珍国医国药,2015,26(1):121-122.
[14]戴敬,杨晓倩,郝培培,等.女金丸薄层色谱鉴别方法研究[J].中成药,2010,32(11):2021-2025.
[15]潘成学,曹泰山,张红岭,等.复方制剂中柴胡薄层层析条件的研究[J].河南科技大学学报,1999,34(2):82-83.
[2]左艇,崔永霞.金银花现代分析方法研究进展[J].河南中医,2009,29(8):831-833.
[3]程丽.中药成分绿原酸与异绿原酸的敏化荧光与分析方法研究[D].河北:河北师范大学,2015.
[4]赵君峰,白志川,刘士尧.绿原酸提取及分析测定方法研究进展[J].辽宁中医药大学学报,2007,9(6):80-82.
[5]尹华,章建华,张春霞,等.芪参健骨颗粒的薄层鉴别研究[J].浙江中医药大学学报,2011,35(1):79-81.
[6]郑弘,许红玮.延胡索不同炮制方法的薄层色谱鉴别对比研究[J].中医学报,2013,28(180):695-696.
[7]丁锤,徐莹,马孝熙,等.柴胡属5种已混药材的鉴别研究[J].中药材,2016,39(9):1975-1981.
[8]贺建华,鲍芳,郑蕊,等.郁舒颗粒的质量标准研究[J].中国实验方剂学杂志,2010,16(2):34-36.
[9]巴小翠,陈金泉,魏春芬,等.复方大青叶合剂中绿原酸TLC方法改进[J].药学实践杂志,2003,21(6):360-361.
[10]李洪.复方芩连清解颗粒的质量控制[J].中国医院药学杂志,2014,10(34):1681-1684.
[11]梁毅,高平,张勤.活血止疼合剂的薄层鉴别研究[J].时珍国医国药,2008,19(9):2210-2212.
[12]朱华,茵潇磊,陈勇.绿原酸、隐绿原酸和新绿原酸在中性和碱性PH条件下的降解动力学[J].药学学报,2016,51(1):122-126.
[13]吴宁,胡俊杰,郑国华,等.益母妇宁胶囊的薄层色谱鉴别[J].时珍国医国药,2015,26(1):121-122.
[14]戴敬,杨晓倩,郝培培,等.女金丸薄层色谱鉴别方法研究[J].中成药,2010,32(11):2021-2025.
[15]潘成学,曹泰山,张红岭,等.复方制剂中柴胡薄层层析条件的研究[J].河南科技大学学报,1999,34(2):82-83.