抗重金属细菌BCD1的分子鉴定及系统发育分析
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摘要
BCD1是一株高度耐受重金属镉的细菌,本研究对其进行形态学、生理生化特性和16SrDNA序列鉴定.结果发现,细菌BCD1革兰氏染色为阴性,细胞呈短杆菌,单个存在,好氧.通过16SrRNA测序Blast比对后发现BCD1和恶臭假单胞菌(Pseudomonas putida)相似性为100%,聚类分析后二者聚为一个分支,由此可以确定菌株BCD1为恶臭假单胞菌(P.putida).
To study the strain BCD1 which was a cadmium resistance bacteria was identified by morphological observation,physiological-biochemical experiments and sequence analysis.The results showed that the bacteria BCD1 were gram negative,and the cells were short bacilli,single and aerobic.After 16 SrRNA sequencing and BLAST comparison,we found that the similarity between BCD1 and Pseudomonas putida was 100%.So the strain BCD1 was identified as Pseudomonas putida by phylogenetic analysis.
To study the strain BCD1 which was a cadmium resistance bacteria was identified by morphological observation,physiological-biochemical experiments and sequence analysis.The results showed that the bacteria BCD1 were gram negative,and the cells were short bacilli,single and aerobic.After 16 SrRNA sequencing and BLAST comparison,we found that the similarity between BCD1 and Pseudomonas putida was 100%.So the strain BCD1 was identified as Pseudomonas putida by phylogenetic analysis.
引文
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[3]呼庆,齐鸿雁,窦敏娜,等.强抗镉蜡状芽孢杆菌的分离鉴定及其抗性机理[J].环境科学,2007,28(2):427-430
[4]白红娟,张肇铭,杨官娥,等.球形红细菌转化去除重金属镉及其机理研究[J].环境科学学报,2006,26(11):1809-1814
[5]李淑英,董诗洁,周元青.重金属胁迫培养对微生物蛋白质含量的影响[J].安徽农业科学,2011,39(31):19051-19053
[6]SRINATH T,VERMA T,RAMTEKE P W,et al.Chromium(VI)biosorption and bioaccumulation br chromate resistant bacteria[J].Chemosphere,2002,48(4):427-435
[7]NAKAJIMA A,YASUDA M.Copper biosorption by chemically treated Micrococcus luteus cells[J].World Journal of Microbiology and Biotechnology,2001,17(4):343-347