人间充质干细胞成脂分化调控机制的研究
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摘要
目的分析人间充质干细胞成脂分化调控机制。方法传统方法培养人间充质干细胞,利用成脂诱导培养基诱导成脂分化,进行基因表达鉴定。结果实验组随着诱导时间的延长,脂滴逐渐显现、含脂滴的细胞数量明显增加,而未加成脂诱导剂的对照组细胞形态始终没有明显变化。实验组第7天、10天、14天的LPL、PPAR-γ基因表达高于对照组(无表达),同时随着时间的延长,表达信号强度明显逐渐上升,提示间充质干细胞成脂化与LPL、PPAR-γ基因表达有关。结论人间充质干细胞成脂分化可能与LPL、PPAR-γ基因表达增强有关。
Objective To analyze the mechanism of adipogenic differentiation of human mesenchymal stem cells. Methods Human mesenchymal stem cells(HUMSCs) were cultured in the traditional method, adipogenic differentiation was induced by adipogenic medium, and gene expression was identified. Results With the prolongation of induction time, the lipid droplets gradually appeared in the experimental group, and the number of cells containing lipid droplets increased significantly. However, the cell morphology of the control group without addition of the lipidinducing agent remained unchanged. The expression of LPL and PPAR-γ on the 7 th, 10 th, and 14 th day in the experimental group was higher than that in the control group(no expression). At the same time, the intensity of the expression signal was gradually increased with time, suggesting the adipogenesis of mesenchymal stem cells and LPL, PPAR-γ gene expression is involved. Conclusion Adipogenic differentiation of human mesenchymal stem cells may be related to enhanced expression of LPL and PPAR-γ genes.
Objective To analyze the mechanism of adipogenic differentiation of human mesenchymal stem cells. Methods Human mesenchymal stem cells(HUMSCs) were cultured in the traditional method, adipogenic differentiation was induced by adipogenic medium, and gene expression was identified. Results With the prolongation of induction time, the lipid droplets gradually appeared in the experimental group, and the number of cells containing lipid droplets increased significantly. However, the cell morphology of the control group without addition of the lipidinducing agent remained unchanged. The expression of LPL and PPAR-γ on the 7 th, 10 th, and 14 th day in the experimental group was higher than that in the control group(no expression). At the same time, the intensity of the expression signal was gradually increased with time, suggesting the adipogenesis of mesenchymal stem cells and LPL, PPAR-γ gene expression is involved. Conclusion Adipogenic differentiation of human mesenchymal stem cells may be related to enhanced expression of LPL and PPAR-γ genes.
引文
[1]蔡敏,林建立,侯建明,等.间充质干细胞的成骨细胞分化和免疫组织化学鉴定[J].解剖学报,2016,47(5):628-632.
[2]赵文涛,李盼盼,张海峰,等.间充质干细胞移植治疗脊髓损伤:系统评价及Meta分析[J].中国组织工程,2015,19(36):5865-5871.
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[9]许海委,徐宝山,杨强,等.细胞共培养法诱导兔脂肪来源干细胞向软骨细胞分化的实验研究[J].中国修复重建外科杂志,2013,27(2):193-198.
[10]周兰庭,冯雁婷,戴景兴,等.mi RNA调控脂肪干细胞分化的研究进展[J].中国修复重建外科杂志,2017,31(12):1506-1511.
[11]刘宇佳,楼斯悦,章燕棋,等.低氧激活自噬促进人间充质干细胞成骨分化的研究[J].中国现代应用药学,2016,33(3):284-289.
[12]赵宇,杨民.脂肪间充质干细胞向成骨细胞分化的研究进展[J].局解手术学价值,2016,25(9):694-696.
[2]赵文涛,李盼盼,张海峰,等.间充质干细胞移植治疗脊髓损伤:系统评价及Meta分析[J].中国组织工程,2015,19(36):5865-5871.
[3]张哲,曾宪卓,鲁菲,等.人间充质干细胞的无异源培养体系研究进展[J].现代生物医学进展,2016,16(34):6793-6796,6672.
[4]李研研,王洪云,马龙乐,等.LPL基因突变与高甘油三酯血症相关性研究进展[J].中华临床医师杂志(电子版),2014,8(2):331-336.
[5]史春民,王拥军,苗登顺.异补骨脂素促进大鼠骨髓间充质干细胞向成骨细胞分化并抑制其向脂肪细胞分化[J].南京医科大学学报(自然科学版),2011,31(5):606-611.
[6]樊奇,郑丹宁,余力,等.SVF细胞影响移植脂肪LPL表达的实验研究[J].组织工程与重建外科杂志,2015,11(3):148-151.
[7]杨芳,陈明月,胡英英,等.PPARγ在脂多糖刺激牙周膜细胞中调控NF-κB信号通路的作用研究[J].口腔医学研究,2017,33(7):698-702.
[8]何洪炳,蔡明成,梁小虎,等.mi R-130b靶向PPARγ抑制家兔前体脂肪细胞分化[J].畜牧兽医学报,2017,48(11):2076-2083.
[9]许海委,徐宝山,杨强,等.细胞共培养法诱导兔脂肪来源干细胞向软骨细胞分化的实验研究[J].中国修复重建外科杂志,2013,27(2):193-198.
[10]周兰庭,冯雁婷,戴景兴,等.mi RNA调控脂肪干细胞分化的研究进展[J].中国修复重建外科杂志,2017,31(12):1506-1511.
[11]刘宇佳,楼斯悦,章燕棋,等.低氧激活自噬促进人间充质干细胞成骨分化的研究[J].中国现代应用药学,2016,33(3):284-289.
[12]赵宇,杨民.脂肪间充质干细胞向成骨细胞分化的研究进展[J].局解手术学价值,2016,25(9):694-696.