摘要
目的探究长链非编码RNA SNHG20在食管鳞状细胞癌患者外周血单个核细胞中的表达及临床意义。方法择取2018年1月至2018年6月郑州大学第二附属医院收治的食管鳞状细胞癌患者40名作为研究组,另择取同期健康体检者30名作为对照组,采用实时定量PCR检测两组患者外周血单个核细胞中SNHG20的相对表达量,比较两组结果差异,分析其在食管鳞癌中的临床意义。结果研究组外周血单个核细胞中SNHG20表达量检测结果低于对照组,差异具有统计学意义(P<0.05),且与食管癌肿瘤分化程度、淋巴结浸润、是否远端转移和TNM分期密切相关。结论食管鳞状细胞癌患者外周血单个核细胞中SNHG20表达变化与其病情之间存在紧密联系,可作为一个潜在的无创性分子指标。
Objective To explore the expression and clinical significance of lncRNA SNHG20 in peripheral blood mononuclear cells of patients with esophageal squamous cell carcinoma. Methods Select 40 patients with esophageal squamous carcinoma as a research team between January 2018 and June 2018 from the Second Affiliated Hospital of Zhengzhou University,the other 30 healthy people as a control. Using real-time quantitative PCR detect the SNHG20 expression quantity in the PBMC of two groups of patients. Compare the difference of results between the two groups and analyze its clinical significance in esophageal cancer. Results The detection results of SNHG20 expression in PBMC of the study group were all lower than those of the control group(P<0.05), which was closely related to the degree of esophageal cancer tumor differentiation,lymph node infiltration, whether distant metastasis and TNM stage.Conclusion The changes of SNHG20 expression in PBMC of patients with esophageal squamous cell carcinoma are closely related to their disease.
引文
[1]邹磊,颉晚林,张鑫,等.LncRNA在胃癌早期诊断中的研究进展[J].国际检验医学杂志,2019,40(1):102-107.
[2] Kai Wang, Juan Li, Gang Xiong, et al. Negative regulation of lncRNA GAS5 by mi R-196a inhibits esophageal squamous cell carcinoma growth[J]. Biochemical and Biophysical Research Communications.2018,495(1):1151-1157.
[3] Min Kang, Meiping Ren, Yan Li, et al. Exosome-mediated transfer of lncRNA PART1 induces gefitinib resistance in esophageal squamous cell carcinoma via functioningas a competing endogenous RNA[J]. Journal of Experimental&Clinical Cancer Research,2018,37:171.
[4] Dongyan Zhang, Chuanhui Cao, Li Liu, et al.Up-regulation of LncRNA SNHG20 Predicts Poor Prognosis in Hepatocellular Carcinoma[J].J Cancer,2016,7(5):609-617.
[5] Liu J, Lu C, Xiao M,et al. Long non-coding RNA SNHG20predicts a poor prognosis for HCC and promotes cell invasion by regulating the epithelial-to-mesenchymal transition[J].Biomed Pharmacother,2017,89(5):857-863.
[6] Chen Z, Chen X, Chen P,et al. Long non-coding RNA SNHG20 promotes non-small cell lung cancer cell proliferation and migration by epigenetically silencing of P21 expression[J]. Cell Death Dis, 2017,8(10):e3092.
[7] He S, Zhao Y, Wang X, et al. Up-regulation of long non-coding RNA SNHG20 promotes ovarian cancer progression via Wnt/β-catenin signaling[J]. Biosci Rep, 2018,38(1)pii:BSR20170681.
[8] Cui N, Liu J, Xia H, et al. LncRNA SNHG20 contributes to cell proliferation and invasion by upregulating ZFXJ expres sion sponging mi R-495-3p in gastric cancer[J]. Cell Biochem,2019,120(3):3114-3123.
[9] Wang W,Luo P,Guo W, et al. LncRNA SNHG20 knockdown suppresses the osteosarcoma tumorigenesis through the mitochondrial apoptosis pathway by mi R-139/RUNX2 axis[J].Biochem Biophys Res Commun.2018, 503(3):1927-1933.
[10] Guo L P, Zhang Z J, Li R T,et al. Influences of LncRNA SNHG20 on proliferation and apoptosis of glioma cells through regulating the PTEN/PI3K/AKT signaling pathway[J]. Rev Med Pharmacol Sci. 2019,23(1):253-261.
[11] Sun C, Sun Y, Zhang E. Long non-coding RNA SNHG20promotes nasopharyngeal carcinoma cell migration and invasion by upregulating TGF-β1[J]. Exp Ther Med, 2018,16(6):4967-4974.
[12] Guo H, Yang S, Li S, et al. LncRNA SNHG20 promotes cell proliferation and invasion via mi R-140-5p-ADAM10 aixs in cervical cancer[J]. Biomed Pharmacother,2018,102:749-757.
[13] Wu J, Zhao W, Wang Z, et al. Long non-coding RNA SNHG20 promotes the tumorigenesis of oral squamous cell carcinoma via targeting mi R-197/LIN28 axis[J]. J Cell Mol Med,2019,23(1):680-688.
[14] Fenoglio C, Oldoni E, Serpente M, et al. LncRNAs expression profile in peripheral blood mononuclear cells from multiple sclerosis patients[J]. J Neuroimmunol, 2018,324:129-135.
[15] YangH,LiangN,WangM,etal.LongnoncodingRNAMALAT-1isanovelinflammatoryregulatorinhumansystemiclupuserythematosus[J].Oncotarget,2017,44(8):77400-77406.