我国部分地区原种猪场3种猪腹泻病毒的血清学调查
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摘要
为了解猪流行性腹泻病毒(PEDV)、猪轮状病毒(PoRV)、猪传染性胃肠炎病毒(TGEV)在我国原种猪场的流行情况,采用ELISA检测方法对2016年1-6月采集的896份发病种猪血清样品进行了血清学检测。结果显示,PEDV、PoRV、TGEV抗体阳性率分别为62.3%,89.0%,23.0%,且存在混合感染情况,其中PEDV+PoRV、PEDV+TGEV、PoRV+TGEV混合感染率分别为42.3%,8.5%,5.6%,PEDV+PoRV+TGEV混合感染率为8.9%;同时对检测样品进行了不同种猪类别的比较和不同日龄段的比较,发现不同类别种猪抗体阳性率和不同日龄种猪抗体阳性率的分布特征显著。结果说明我国原种猪场3种腹泻病毒中PoRV感染率最高,其次是PEDV和TGEV,且在不同种猪类别和不同日龄段的种猪中呈现规律性分布。本试验对国内原种猪场猪血清进行PEDV、PoRV和TGEV抗体阳性率检测,为我国种猪病毒性腹泻的防控提供了参考。
To investigate the prevalence of porcine epidemic diarrhea virus(PEDV),porcine rotavirus virus(PoRV)and transmissible gastroenteritis virus(TGEV)in breeding pig farm of China,a total of 896 serum samples were collected from January 2016 to June 2016 and ELISA methods were used to detect the antibodys against related viruses.The result showed that the positive rates of the antibodys against PEDV,PoRV and TGEV were 62.3%,89.0%and 23.0%,respectively.The mixed infection rate of PEDV+PoRV,PEDV+TGEV and PoRV+TGEV were 42.3%,8.5% and 5.6% respectively and PEDV+PoRV+TGEV were 8.9%.Types and space analysis of the samples showed that they were distributed regularly.The result showed that the PoRV infection rate was higher than that of PEDV and TGEV in pig breeding farms.It provides reference information for the prevention and control of swine virus diarrhea.
To investigate the prevalence of porcine epidemic diarrhea virus(PEDV),porcine rotavirus virus(PoRV)and transmissible gastroenteritis virus(TGEV)in breeding pig farm of China,a total of 896 serum samples were collected from January 2016 to June 2016 and ELISA methods were used to detect the antibodys against related viruses.The result showed that the positive rates of the antibodys against PEDV,PoRV and TGEV were 62.3%,89.0%and 23.0%,respectively.The mixed infection rate of PEDV+PoRV,PEDV+TGEV and PoRV+TGEV were 42.3%,8.5% and 5.6% respectively and PEDV+PoRV+TGEV were 8.9%.Types and space analysis of the samples showed that they were distributed regularly.The result showed that the PoRV infection rate was higher than that of PEDV and TGEV in pig breeding farms.It provides reference information for the prevention and control of swine virus diarrhea.
引文
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[13]朱文渊,郝晴晴,霍翠梅,等.仔猪流行性腹泻病毒原性调查研究[J].中国农业信息,2016(11):41-42.
[14]韩莎莎,张玉娟,时庆贺,等.河南省2014-2015年猪流行性腹泻病毒流行株遗传进化分析[J].中国兽医学报,2017,37(6):976-983.
[15]李加飞,陈智,孙文博,等.山东地区猪流行性腹泻病毒遗传变异分析及其单克隆抗体的制备[J].中国兽医学报,2017,37(5):769-775.
[16]白小磊,崔进,崔甜甜,等.广东部分地区猪流行性腹泻病毒流行病学调查[J].中国兽医学报,2016,36(11):1823-1828.
[17]王飞,陈小芬,苏丹萍,等.2014-2015年我国部分省份猪流行性腹泻病毒S基因的遗传变异分析[J].中国兽医学报,2016,36(9):1484-1489.
[18]STRAW B E,ZIMMERMAN J J,ALLAIRE S D,等.赵德明,张仲秋,沈建忠主译.猪病学[M].9版.北京:中国农业大学出版社,2000:481-494.
[19]PARASHAR U D,HUMMELMAN E G,BRESEE JS,et al.Global illness and deaths caused by rotavirus disease in children[J].Emerg Infect Dis,2003,9(5):565-572.
[2]李丽敏,朱卫霞,王晓波,等.猪流行性腹泻病毒、猪传染性胃肠炎病毒、猪A型轮状病毒多重RT-PCR方法的建立及其应用[J].中国兽医学报,2016,36(2):216-220.
[3]WOOD E N.An apparently new syndrome of porcine epidemic diarrhoea[J].Vet Record,1977,100(12):243-244.
[4]VLASOVA A N,MARTHALER D,WANG Q,et al.Distinct characteristics and complex evolution of PEDV strains,North America,May 2013-February2014[J].Emerg Infect Dis,2014,20(10):1620-1628.
[5]STEVENSON G W,HOANG H,SCHWARTZ K J,et al.Emergence of Porcine epidemic diarrhea virus in the United States:clinical signs,lesions,and viral genomic sequences[J].J Vet Diagn Invest,2013,25(5):649-654.
[6]翁善钢.2014年国际猪病疫情回顾---猪流行性腹泻在全球的流行与传播[J].肉类工业,2015(2):49-50.
[7]甘振磊,汤德元,李春燕,等.猪流行性腹泻流行特点及流行现状的研究[J].猪业科学,2010,27(12):24-28.
[8]SUN R Q,CAI R J,CHEN Y Q,et al.Outbreak of porcine epidemic diarrhea in suckling piglets,China[J].Emerg Infect Dis,2012,18(1):161-163.
[9]田冲,欧阳金旭,罗碧毅,等.仔猪病毒性腹泻的流行病学研究进展[J].长江大学学报(自然科学版),2013,10(5):57-60.
[10]穆杨.猪传染性胃肠炎疫苗研究进展[J].动物医学进展,2011,32(2):91-96.
[11]薛瑞雪,田野,田夫林,等.山东省部分地区仔猪病毒性腹泻流行病学调查[J].中国预防兽医学报,2015,37(4):254-257.
[12]刘艳成,杜雅楠,吴卫杰,等.内蒙古地区猪腹泻相关病毒的检测与分析[J].畜牧兽医学报,2015,46(9):1620-1628.
[13]朱文渊,郝晴晴,霍翠梅,等.仔猪流行性腹泻病毒原性调查研究[J].中国农业信息,2016(11):41-42.
[14]韩莎莎,张玉娟,时庆贺,等.河南省2014-2015年猪流行性腹泻病毒流行株遗传进化分析[J].中国兽医学报,2017,37(6):976-983.
[15]李加飞,陈智,孙文博,等.山东地区猪流行性腹泻病毒遗传变异分析及其单克隆抗体的制备[J].中国兽医学报,2017,37(5):769-775.
[16]白小磊,崔进,崔甜甜,等.广东部分地区猪流行性腹泻病毒流行病学调查[J].中国兽医学报,2016,36(11):1823-1828.
[17]王飞,陈小芬,苏丹萍,等.2014-2015年我国部分省份猪流行性腹泻病毒S基因的遗传变异分析[J].中国兽医学报,2016,36(9):1484-1489.
[18]STRAW B E,ZIMMERMAN J J,ALLAIRE S D,等.赵德明,张仲秋,沈建忠主译.猪病学[M].9版.北京:中国农业大学出版社,2000:481-494.
[19]PARASHAR U D,HUMMELMAN E G,BRESEE JS,et al.Global illness and deaths caused by rotavirus disease in children[J].Emerg Infect Dis,2003,9(5):565-572.