经脉不通对大鼠行为学及胃肠动力的影响
|
摘要
目的:采用行为学以及胃肠动力指标对大鼠经脉不通证候模型进行评价,为探索中医经络病候模型提供评价方法和基础。方法:采用随机数字表法将41只大鼠随机分为空白对照组10只、非经结扎组10只,胃经结扎组10只和肾经结扎组11只。空白对照组大鼠只进行麻醉处理,其余3组大鼠均用结扎法在相应的非经脉线或经脉线上进行结扎堵塞低流阻通道造模。采用旷场实验、糖水偏好实验观察行为学变化,通过计算胃残留率及小肠推进率观察胃肠动力变化。结果:胃经结扎组大鼠在造模后第1周行为学得分显著低于空白对照组(P<0.05),在造模后第4、5周显著高于空白对照组(P<0.05);肾经结扎组大鼠在造模后第1、2、4、5周行为学得分显著低于空白对照组(P<0.01或P<0.05);胃经结扎组大鼠在造模后第1周行为学得分显著低于非经结扎组(P<0.05),第5周显著高于非经结扎组(P<0.05);肾经结扎组大鼠在造模后每周行为学得分均显著低于非经结扎组(P<0.05或P<0.01)。胃经结扎组和肾经结扎组大鼠在造模后第1周中央区停留时间均显著少于空白对照组和非经结扎组(P<0.05)。胃经结扎组大鼠在造模后糖水偏好值明显高于空白对照组和非经结扎组(P<0.01)。胃经结扎组大鼠小肠推进率明显高于空白对照组和非经结扎组(P<0.05)。结论:结扎堵塞大鼠足阳明胃经与足少阴肾经循经低流阻通道,可导致与胃经、肾经病候相关的情志状态与胃肠动力改变,说明结扎法能引起大鼠相对特异性变化。本实验为经络疾病证候模型提供了评价手段和基础。
Objective: Behavioral and gastrointestinal motility indexes were used to evaluate the syndrome model of meridian obstruction in rats, which provided evaluation methods and basic information for exploring the syndrome model of meridian diseases in TCM. Method: 41 male SD rats were randomly divided into control grou p(CG, n=10), Ligation on non-meridian group(LNMG, n=10), Ligation on The Stomach Meridian group(LSTMG,n=10) and Ligation on The Kidney Meridian group(LKIMG, n=11). The CG was only given anesthesia. The other three groups were tied on the non-meridian area or meridian area to blocking the low hydraulic resistance channels(LHRC). Weekly open field and sugar water preference experiment which was observed before and after modeling were conducted to observe behavioral changes in each group. Gastrointestinal motility index including gastric residual rate and small intestinal propulsion rate were observed after modeling. Results: In behavior score aspect, compared with the CG, the LSTMG was decreased significantly in the first week(P<0.05), and increased significantly in the fourth and fifth weeks(P<0.05); the LKIMG was decreased significantly in the first, second,
Objective: Behavioral and gastrointestinal motility indexes were used to evaluate the syndrome model of meridian obstruction in rats, which provided evaluation methods and basic information for exploring the syndrome model of meridian diseases in TCM. Method: 41 male SD rats were randomly divided into control grou p(CG, n=10), Ligation on non-meridian group(LNMG, n=10), Ligation on The Stomach Meridian group(LSTMG,n=10) and Ligation on The Kidney Meridian group(LKIMG, n=11). The CG was only given anesthesia. The other three groups were tied on the non-meridian area or meridian area to blocking the low hydraulic resistance channels(LHRC). Weekly open field and sugar water preference experiment which was observed before and after modeling were conducted to observe behavioral changes in each group. Gastrointestinal motility index including gastric residual rate and small intestinal propulsion rate were observed after modeling. Results: In behavior score aspect, compared with the CG, the LSTMG was decreased significantly in the first week(P<0.05), and increased significantly in the fourth and fifth weeks(P<0.05); the LKIMG was decreased significantly in the first, second,
引文
[1]战晓玲,李沧海,姜廷良.表证模型研究概况与展望[J].中国实验方剂学杂志,2018,24(19):213-220.
[2]孙剑端,任路,李静,等.电针对胃溃疡肝郁证模型大鼠镇痛作用研究[J].中国针灸,2015,35(4):361-366.
[3]徐菁,芦娟,郭妍,等.针刀干预对帕金森病大鼠行为学和纹状体神经递质的影响[J].针灸临床杂志,2017,33(11):58-62.
[4]佘燕玲,江婷,陈睿,等.电针百会印堂对抑郁模型大鼠行为学及海马超微结构的影响[J].中国中医基础医学杂志,2017,23(6):837-839.
[5] Zhang W B, Tian Y Y,Li H, et al. A Discovery of Low hydraulic Resistance Channel along Meridians[J].Acup Merid Res,2008,1(1):20-28.
[6] Wang Z,Zhang W,Jia S,et al. Finding Blue Tracks in Gephyrocharax melanocheir,Fish Similar to the Locations ofAcupuncture Meridians after Injecting Alcian Blue[J].Journal of Acupuncture&Meridian Studies,2015,8(6):307-313.
[7]徐一慧,张维波,田宇瑛,等.奥美定水凝胶注射对循经低流阻通道阻断作用的初步观察[J].生物医学工程学杂志,2009,26(4):776-779
[8]张宇沁,田宇瑛,丁玉龙,等.实验用小型猪行为学应用于中医基础研究的方法学探讨[J].实验动物科学,2011,28(2):47-51.
[9]李宏彦,王燕平,佘锐萍,等.小型猪胃经与肾经经脉不通病理模型的比较观察[J].中国中医基础医学杂志,2018,24(2):176-179,231.
[10]黄琰,王燕平,张维波,等.使用结扎法建立大鼠经脉不通病理模型的初步研究[J].中国中医基础医学杂志,2018,24(5):592-595.
[11] Prut L, Belzung C. The open field as a paradigm to measure the effects of drugs on anxiety-like behaviors:a review[J].European Journal of Pharmacology,2003,463(1):3-33.
[12]魏盛,刘伊娜,杜希扬.情绪反应动物模型的研究进展[J].医学综述,2011,17(22):3373-3376.
[13]石学敏,韩景献,秦文宇.试论《灵枢·经脉》篇“是动”和“所生”病[J].中国针灸,1988,8(2):44-47.
[14]石学敏.石学敏针灸临证集验[M].天津:天津科学技术出版社,1990:526-562.
[15]李伟,詹向红,徐玮玮,等.慢性愤怒应激加速脑老化大鼠模型建立及行为学评价[J].中国老年学杂志,2010,30(23):3509-3511.
[16] Pothion S,Bizot J C, Trovero F, et al. Strain differences in sucrose preference and in the consequences of unpredictable chronic mild stress[J].Behavioural Brain Research,2004,155(1):135-146.
[17]魏盛,高杰,乔明琦.情志病证动物模型行为学评价应遵循的基本原则[J].山东中医药大学学报,2013,37(6):462-463.
[18]王德军,常小荣,严洁,等.针刺足阳明胃经特定穴与非特定穴治疗功能性消化不良疗效比较[J].中国针灸,2012,32(8):703-708.
[2]孙剑端,任路,李静,等.电针对胃溃疡肝郁证模型大鼠镇痛作用研究[J].中国针灸,2015,35(4):361-366.
[3]徐菁,芦娟,郭妍,等.针刀干预对帕金森病大鼠行为学和纹状体神经递质的影响[J].针灸临床杂志,2017,33(11):58-62.
[4]佘燕玲,江婷,陈睿,等.电针百会印堂对抑郁模型大鼠行为学及海马超微结构的影响[J].中国中医基础医学杂志,2017,23(6):837-839.
[5] Zhang W B, Tian Y Y,Li H, et al. A Discovery of Low hydraulic Resistance Channel along Meridians[J].Acup Merid Res,2008,1(1):20-28.
[6] Wang Z,Zhang W,Jia S,et al. Finding Blue Tracks in Gephyrocharax melanocheir,Fish Similar to the Locations ofAcupuncture Meridians after Injecting Alcian Blue[J].Journal of Acupuncture&Meridian Studies,2015,8(6):307-313.
[7]徐一慧,张维波,田宇瑛,等.奥美定水凝胶注射对循经低流阻通道阻断作用的初步观察[J].生物医学工程学杂志,2009,26(4):776-779
[8]张宇沁,田宇瑛,丁玉龙,等.实验用小型猪行为学应用于中医基础研究的方法学探讨[J].实验动物科学,2011,28(2):47-51.
[9]李宏彦,王燕平,佘锐萍,等.小型猪胃经与肾经经脉不通病理模型的比较观察[J].中国中医基础医学杂志,2018,24(2):176-179,231.
[10]黄琰,王燕平,张维波,等.使用结扎法建立大鼠经脉不通病理模型的初步研究[J].中国中医基础医学杂志,2018,24(5):592-595.
[11] Prut L, Belzung C. The open field as a paradigm to measure the effects of drugs on anxiety-like behaviors:a review[J].European Journal of Pharmacology,2003,463(1):3-33.
[12]魏盛,刘伊娜,杜希扬.情绪反应动物模型的研究进展[J].医学综述,2011,17(22):3373-3376.
[13]石学敏,韩景献,秦文宇.试论《灵枢·经脉》篇“是动”和“所生”病[J].中国针灸,1988,8(2):44-47.
[14]石学敏.石学敏针灸临证集验[M].天津:天津科学技术出版社,1990:526-562.
[15]李伟,詹向红,徐玮玮,等.慢性愤怒应激加速脑老化大鼠模型建立及行为学评价[J].中国老年学杂志,2010,30(23):3509-3511.
[16] Pothion S,Bizot J C, Trovero F, et al. Strain differences in sucrose preference and in the consequences of unpredictable chronic mild stress[J].Behavioural Brain Research,2004,155(1):135-146.
[17]魏盛,高杰,乔明琦.情志病证动物模型行为学评价应遵循的基本原则[J].山东中医药大学学报,2013,37(6):462-463.
[18]王德军,常小荣,严洁,等.针刺足阳明胃经特定穴与非特定穴治疗功能性消化不良疗效比较[J].中国针灸,2012,32(8):703-708.