黄芩素对喉癌细胞周期、凋亡率及细胞核DNA的影响
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摘要
目的:探索黄芩素(baicalein,BAI)对喉癌细胞周期、凋亡率及细胞核DNA的影响。方法:体外培养的Hep-2细胞,加入不同浓度的BAI,药物作用后,用MTT法检测细胞增殖情况;用流式细胞术检测对细胞周期及细胞凋亡率的影响;用DNA琼脂糖凝胶电泳法检测对凋亡细胞核DNA的影响。结果:MTT法检测到BAI(10、20、40、80μmol/L)可明显抑制Hep-2细胞的增殖,呈时间-剂量依赖性(P<0.05);FCM法检测到BAI(40μmol/L)可诱使细胞阻滞在S期,凋亡率升高(P<0.05);BAI(40、80μmol/L)可诱使细胞核DNA形成"梯状"条带;呈时间-剂量依赖性(P<0.05)。结论:一定浓度的BAI,可抑制人喉癌Hep-2细胞增殖,诱导细胞阻滞在S期,细胞凋亡率升高,晚期凋亡细胞核DNA断裂形成DNA Ladder。
Objective:To investigate the effect of Baicalein(BAI) on cell cycle,apoptosis rate,DNA of apoptotic cells in laryngealcancer cells.Method:Hep-2 cells were exposed to BAI at various concentrations respectively,in vitro.MTT assay was used to determine cell proliferation;flow cytometry were used to analyze cell cycle and apoptosis rate;the effect on the DNA was detected by agarose gel electrophoresis.Result:MTT assay showed that the proliferation of Hep-2 cells were significantly inhibited by BAI(10,20,40 and 80 μmol/L),in a time and dose dependent manner(P<0.05).Hep-2 cells are arrested at the S phase and the apoptosis rate was significantly increased by BAI(40 μmol/L)through flow cytometry(P<0.05).DNA ladder in Hep-2 cells was induced by BAI(40,80 μmol/L).Conclusion:BAI can inhibite proliferation of Hep-2 cells,increase the apoptosis rate,induce Hep-2 cells in S phase and late apoptotic cells DNA broken into DNA Ladder.
Objective:To investigate the effect of Baicalein(BAI) on cell cycle,apoptosis rate,DNA of apoptotic cells in laryngealcancer cells.Method:Hep-2 cells were exposed to BAI at various concentrations respectively,in vitro.MTT assay was used to determine cell proliferation;flow cytometry were used to analyze cell cycle and apoptosis rate;the effect on the DNA was detected by agarose gel electrophoresis.Result:MTT assay showed that the proliferation of Hep-2 cells were significantly inhibited by BAI(10,20,40 and 80 μmol/L),in a time and dose dependent manner(P<0.05).Hep-2 cells are arrested at the S phase and the apoptosis rate was significantly increased by BAI(40 μmol/L)through flow cytometry(P<0.05).DNA ladder in Hep-2 cells was induced by BAI(40,80 μmol/L).Conclusion:BAI can inhibite proliferation of Hep-2 cells,increase the apoptosis rate,induce Hep-2 cells in S phase and late apoptotic cells DNA broken into DNA Ladder.
引文
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[16]万书丽,庞鑫,东野广智,等.不同剂量地塞米松对小鼠胸腺细胞凋亡的影响[J].广东医学院学报,2015,33(1):35-44.
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[18]Zhang N,Wang W,Li W,et al.Inhibition of 11β-HSD2 expression by triclosan via induction of apoptosis in human place ntalsyncytiotrophoblasts[J].Clin Endocrinol Metab,2015,100(4):E542-E549.
[19]李敏,林俊.细胞凋亡途径及其机制[J].国际妇产科学杂志,2014,41(2):103-107.
[20]郭茜茜,于广,吴丹丹,等.细胞凋亡率检测方法的探索[J].免疫学杂志,2015,35(5):425-429.
[21]周国运,王熙,曹干生,等.双氢青蒿素对肺癌细胞增殖的抑制作用[J].中国老年学杂志,2017,37(8):1874-1876.
[2]于振坤,张庆翔,龚单春,等.早期喉癌的治疗选择[J].临床误诊误治,2014,27(9):6-8.
[3]胡小琴,吴可明,郑国峰.老年喉癌患者手术治疗的远期疗效及预后的影响因素[J].中国老年学杂志,2017,37(12):2972-2974.
[4]龚虹云,宋启斌,胡伟国,等.首选化放疗或手术治疗喉癌疗效的系统评价[J].循证医学,2014,14(4):229-233.
[5]张亚娜.喉部分切除术与全喉切除术治疗晚期喉癌的Meta分析[D].太原:山西医科大学,2017.
[6]谢芳.236例喉癌患者手术治疗生存效果分析[D].上海:上海交通大学,2014.
[7]马士崟,王晓敏,韩跃峰,等.381例喉癌保留喉功能的手术治疗[J].中国中西医结合耳鼻咽喉科杂志,2016,24(5):347-352.
[8]田硕,洪涛,张多,等.黄芩素的药理作用及分子机制的最新研究进展[J].黑龙江医药,2015,28(6):1195-1199.
[9]肖灵芝.中药黄芩的药理和应用[J].中医临床研究,2014,6(3):133-134.
[10]郑勇凤,王佳婧,傅超美,等.黄芩的化学成分与药理作用研究进展[J].中成药,2016,38(1):141-147.
[11]Ma G Z,Liu C H,Wei B,et al.Baicalein inhibits DMBA/TPAinduced skin tumorigenesis in mice by modulating proliferation,apoptosis,and inflammation[J].Inflammation,2013,36(2):457-467.
[12]刘梅,曲乐,尹新江,等.黄芩素联合阿维A酸对皮肤鳞状细胞癌细胞SCL-12凋亡Caspa-ses的影响[J].中国免疫学杂志,2014,30(3):353-356.
[13]孙吉凤,刘诗音,宋英明.黄芩素抗肿瘤作用机制的研究进展[J].中国当代医药,2015,22(14):24-26.
[14]张淑群,高晓燕,薛兴欢,等.黄芩素对人乳腺癌MDAMB-231细胞株SATB1表达的影响[J].中国肿瘤临床,2014,41(6):355-358.
[15]孙吉凤,刘剑凯,张淑芳.黄芩素对喉癌Hep-2细胞增殖的抑制作用[J].中国医学创新,2015,12(21):4-6.
[16]万书丽,庞鑫,东野广智,等.不同剂量地塞米松对小鼠胸腺细胞凋亡的影响[J].广东医学院学报,2015,33(1):35-44.
[17]Suman S,Pandey A,Chandna S.An improved nonenzymatic“DNA ladder assay”for more sensitive and early detection of apoptosis[J].Cytotechnology,2012,64(1):9-14.
[18]Zhang N,Wang W,Li W,et al.Inhibition of 11β-HSD2 expression by triclosan via induction of apoptosis in human place ntalsyncytiotrophoblasts[J].Clin Endocrinol Metab,2015,100(4):E542-E549.
[19]李敏,林俊.细胞凋亡途径及其机制[J].国际妇产科学杂志,2014,41(2):103-107.
[20]郭茜茜,于广,吴丹丹,等.细胞凋亡率检测方法的探索[J].免疫学杂志,2015,35(5):425-429.
[21]周国运,王熙,曹干生,等.双氢青蒿素对肺癌细胞增殖的抑制作用[J].中国老年学杂志,2017,37(8):1874-1876.