摘要
目的优化油茶果壳多糖乙醇提取工艺,并评价其吸湿保湿性能。方法在单因素试验基础上,以乙醇体积分数、料液比、提取时间、提取次数为影响因素,多糖得率为评价指标,正交试验优化提取工艺。然后,以海藻酸钠为对照,在相对湿度43%、81%下测定48 h内吸湿率,在相对湿度34%下测定48 h内保湿率。结果最佳条件为乙醇体积分数60%,粉末粒径80目,提取温度100℃,料液比1∶35,提取时间1.5 h,提取2次,多糖得率5.42%。在相对湿度43%、81%下多糖吸湿率分别为30.5%、50.1%,在相对湿度34%下其保湿率为20.8%,与海藻酸钠接近。结论该方法稳定可行,可用于乙醇提取吸湿保湿性能较好的油茶果壳多糖。
AIM To optimize the ethanol extraction process for polysaccharides from Camellia oleifera Abel. fruit shells and to evaluate their moisture-absorption, moisture-retention properties.METHODS With ethanol concentration, solid-liquid ratio, extraction time and extraction frequency as influencing factors, polysaccharides yield as an evaluation index, the extraction process was optimized by orthogonal test on the basis of single factor test. Subsequently, with sodium alginate as a control, moisture-absorption rates within 48 h under the relative humidities of 43% and 81%, and moisture-retention rate within 48 h under the relative humidity of 34% were determined, respectively.RESULTS The optimal conditions were determined to be 60% for ethanol concentration, 80 mesh for powder particle size, 100 ℃ for extraction temperature, 1∶35 for solid-liquid ratio, 1.5 h for extraction time, and extraction twice, the polysaccharides yield was 5.42%. The polysaccharides demonstrated the moisture-absorption rates of 30.5% and 50.1% under the relative humidities of 43% and 81%, respectively, with the moisture-retention rate of 20.8% under the relative humidity of 34%, which approximated those of sodium alginate.CONCLUSION This stable and feasible method can be used for the ethanol extraction for polysaccharides with good moisture-absorption, moisture-retention properties from C. oleifera fruit shells.
引文
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