还原型谷胱甘肽辅助治疗卵巢癌术后化疗对机体免疫功能、抗氧化功能及炎症因子水平的影响
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摘要
目的:探讨还原型谷胱甘肽辅助治疗卵巢癌术后化疗对机体免疫功能、抗氧化功能及炎症因子水平的影响。方法:选择卵巢癌术后患者100例,根据入组序列号分为研究组与对照组各50例,其中对照组给予常规化学药物治疗,研究组在化学药物治疗的基础上给予还原型谷胱甘肽辅助治疗,观察两组化疗前(T0)、化疗1个月(T1)、3个月后(T2)T淋巴细胞亚群、抗氧化功能指标MDA、SOD、T-AOC及炎症因子TNF-α、IL-6、IL-8水平变化。结果:两组CD3~+、CD4~+、CD4~+/CD8~+水平T1较T0下降,T2较T1明显升高,CD8~+水平T1较T0升高,T2较T1明显下降,研究组T1、T2CD3~+、CD4~+、CD4~+/CD8~+水平高于对照组,CD8~+水平低于对照组,差异均有统计学意义(P<0.05);两组MDA水平T1较T0升高,T2较T1明显下降,SOD、T-AOC水平T1较T0下降,T2较T1明显升高,研究组T1、T2SOD、T-AOC水平高于对照组,MDA水平低于对照组,差异均有统计学意义(P<0.05);两组TNF-α、IL-6、IL-8水平T1较T0升高,T2较T1明显下降,研究组T1、T2、TNF-α、IL-6、IL-8水平低于对照组,差异均有统计学意义(P<0.05)。结论:还原型谷胱甘肽辅助治疗卵巢癌术后化疗有助于保护机体免疫功能、抗氧化功能,减轻炎症反应。
Objective:To investigate the effect of reduced glutathione on immune function,antioxidant function and inflammatory cytokines in ovarian cancer patients after chemotherapy.Result:100patients with ovarian cancer who were admitted from January 2015to June 2017.According to the enrollment serial number were divided into study group and control group each 50cases,the control group was given conventional chemical drug treatment,the research group were given reduced glutathione adjuvant therapy on the basis of chemical therapy.To observe The T lymphocyte subsets,antioxidant indexes MDA,SOD,T-AOC and inflammatory cytokines TNF-αand IL-6in two groups before chemotherapy(T0),1 month after chemotherapy(T1)and 3months 6,IL-8levels change.Result:(1)The levels of T1with The CD3~+,CD4~+,CD4~+/CD8~+was lower than the T0,and T2was significantly higher than the T1,The CD8~+level in T1was higher than the T0,and the T2was significantly lower than the T1in both groups.The levels of the CD3~+,CD4~+,CD4~+/CD8with T1,T2in the study group were higher than the control group,while the levels of CD8~+in the study group were lower than the control group.So the difference was statistically significant(~(CD 3+)t_(T1)=5.318,~(CD 3+)t_(T2)=8.356,~(CD 4+)t_(T1)=4.773,~(CD 4+)t_(T2)=6.052,~(CD 8+)t_(T1)=5.339,~(CD 8+)t_(T2)=10.158,~(CD 4+/CD 8+)t_(T1)=4.092,~(CD 4+/CD 8+)t_(T2)=4.004and P<0.05).(2)The level of MDA in T1was higher than in the T0and the T2was significantly lower than the T1in both groups.The levels of SOD and T-AOC in T1were decreased from T0,and in T2was significantly higher than T1.The levels of T1,T2with the SOD and T-AOC in the study group were higher than in the control group,while the MDA level was lower than in the control group.And the difference was statistically significant(~(M D A)t_(T1)=49.164,~(M D A)t_(T2)=35.274,~(S O D)t_(T1)=44.187,~(S O D)t_(T2)=56.376,~(T-A O C)t_(T1)=11.442,~(T-A O C)t_(T2)=17.534,so P<0.05).(3)The levels of TNF-α,IL-6and IL-8with T1in two groups were significantly higher than in T0,and the T2decreased significantly compared with T1,The levels of T1and T2with TNF-α,IL-6and IL-8in the study group were lower than in the control group.So the difference was statistically significant(~(T N F-α)t_(T1)=60.442,~(T N F-α)t_(T2)=51.309,~(I L-6)t_(T1)=78.365,~(I L-6)t_(T2)=59.143,~(I L-8)t_(T1)=19.235,~(I L-8)t_(T2)=17.998,P<0.05).Conclusion:These will help to protect the body's immune function and antioxidant function and reduce inflammation factor with in the reduced glutathione adjuvant chemotherapy for postoperative ovarian cancer.
Objective:To investigate the effect of reduced glutathione on immune function,antioxidant function and inflammatory cytokines in ovarian cancer patients after chemotherapy.Result:100patients with ovarian cancer who were admitted from January 2015to June 2017.According to the enrollment serial number were divided into study group and control group each 50cases,the control group was given conventional chemical drug treatment,the research group were given reduced glutathione adjuvant therapy on the basis of chemical therapy.To observe The T lymphocyte subsets,antioxidant indexes MDA,SOD,T-AOC and inflammatory cytokines TNF-αand IL-6in two groups before chemotherapy(T0),1 month after chemotherapy(T1)and 3months 6,IL-8levels change.Result:(1)The levels of T1with The CD3~+,CD4~+,CD4~+/CD8~+was lower than the T0,and T2was significantly higher than the T1,The CD8~+level in T1was higher than the T0,and the T2was significantly lower than the T1in both groups.The levels of the CD3~+,CD4~+,CD4~+/CD8with T1,T2in the study group were higher than the control group,while the levels of CD8~+in the study group were lower than the control group.So the difference was statistically significant(~(CD 3+)t_(T1)=5.318,~(CD 3+)t_(T2)=8.356,~(CD 4+)t_(T1)=4.773,~(CD 4+)t_(T2)=6.052,~(CD 8+)t_(T1)=5.339,~(CD 8+)t_(T2)=10.158,~(CD 4+/CD 8+)t_(T1)=4.092,~(CD 4+/CD 8+)t_(T2)=4.004and P<0.05).(2)The level of MDA in T1was higher than in the T0and the T2was significantly lower than the T1in both groups.The levels of SOD and T-AOC in T1were decreased from T0,and in T2was significantly higher than T1.The levels of T1,T2with the SOD and T-AOC in the study group were higher than in the control group,while the MDA level was lower than in the control group.And the difference was statistically significant(~(M D A)t_(T1)=49.164,~(M D A)t_(T2)=35.274,~(S O D)t_(T1)=44.187,~(S O D)t_(T2)=56.376,~(T-A O C)t_(T1)=11.442,~(T-A O C)t_(T2)=17.534,so P<0.05).(3)The levels of TNF-α,IL-6and IL-8with T1in two groups were significantly higher than in T0,and the T2decreased significantly compared with T1,The levels of T1and T2with TNF-α,IL-6and IL-8in the study group were lower than in the control group.So the difference was statistically significant(~(T N F-α)t_(T1)=60.442,~(T N F-α)t_(T2)=51.309,~(I L-6)t_(T1)=78.365,~(I L-6)t_(T2)=59.143,~(I L-8)t_(T1)=19.235,~(I L-8)t_(T2)=17.998,P<0.05).Conclusion:These will help to protect the body's immune function and antioxidant function and reduce inflammation factor with in the reduced glutathione adjuvant chemotherapy for postoperative ovarian cancer.
引文
1张勇.DC-CIK辅助治疗对老年晚期卵巢癌病人T细胞亚群和生活质量的影响[J].实用老年医学,2017,31(3):257-260.
2 Li L,Bai H,Yang J,et al.Genome-wide DNA copy number analysis in clonally expanded human ovarian cancer cells with distinct invasive/migratory capacities[J].Oncotarget,2017,8(9):15136-15148.
3 Chang SJ,Bristow RE,Chi DS,et al.Role of aggressive surgical cytoreduction in advanced ovarian cancer[J].J Gynecol Oncol,2015,26(4):336-342.
4 乔志伟,王纯雁,王蕾,等.新辅助化疗联合肿瘤细胞减灭术中热灌注治疗晚期卵巢癌疗效观察[J].湖南师范大学学报(医学版),2015,12(1):83-85.
5 王秀娟,苑中甫,邱海峰,等.术前外周血淋巴细胞/单核细胞比值与上皮性卵巢癌患者预后的关系[J].现代妇产科进展,2016,25(9):654-657.
6 乔红丽,侯炜,李站,等.朴炳奎治疗卵巢癌辨证思路及用药规律总结[J].北京中医药,2014,33(10):735-738.
7 Cheng AY,Zhang Y,Mei HJ,et al.Construction of a plasmid for overexpression of human circadian gene peri-od2and its biological activity in osteosarcoma cells[J].Tumour Biol,2015,36(5):3735-3743.
8 黄景慧,尤冬山,陈欣,等.晚期乳腺癌患者行FAC方案化疗对体内氧化应激水平的影响[J].疑难病杂志,2017,16(4):377-380.
9 杨昆,王学文,赖钊,等.射频消融联合肝动脉化疗栓塞对HCC患者炎性、氧化应激反应及肿瘤活性因子指标影响[J].海南医学院学报,2017,23(4):531-534.
10 Fan XP,Ji XF,Li XY,et al.Methylation of the GlutathioneStransferase P1 gene promoter is associated with oxidative stress in patients with chronic hepatitis B[J].Tohoku J Exp Me,2016,238(1):57-64.
11 柏志强,付熠,黄京璐,等.还原性谷胱甘肽治疗对非ST段抬高型心肌梗死患者近期预后及血浆硫氧还蛋白水平变化的影响[J].热带医学杂志,2015,15(4):507-510.
12 张哲英,张蓓,秦静,等.多器官功能障碍综合征患者血清对内皮细胞氧化损伤的影响及还原型谷胱甘肽的保护作用[J].中国急救医学,2015,35(1):38-43.
13 Razi Soofiyani S,Kazemi T,Lotfipour F,et al.Gene therapy with IL-12induced enhanced anti-tumor activity in fibrosarcoma mouse model[J].Artif Cells Nanomed Biotechnol.2016,44(8):1988-1993.
14 陈立,韩海滨,李佳,等.还原型谷胱甘肽对2型糖尿病早期肾病患者血浆Hcy、尿清蛋白排泄率、甲状腺功能及炎症因子的影响[J].中国中西医结合肾病杂志,2016,17(4):349-351.
15 辛光大,周广宇.还原型谷胱甘肽对老年早期糖尿病肾病患者氧化应激和炎症反应的影响[J].中国老年学杂志,2014,34(6):1449-1450.
16 方芳,赵良中,李强,等.胡桃醌对卵巢癌SKOV3细胞氧化应激和凋亡的影响[J].中国病理生理杂志,2016,32(1):156-159.
17 Qi X,Li Y,Rahe-Meyer N,et al.Intrathecal dexmedetomidine as adjuvant to ropivacaine in hysteroscopic surgery:aprospective,randomized control study[J].Int J Clin Pharmacol Ther,2016,54(3):185-192.
18 柳永兵.还原型谷胱甘肽对尿毒症维持性血液透析患者微炎症状态的影响[J].中国药房,2015,26(12):1637-1639.
19 李超,陈桂明,彭冰,等.还原型谷胱甘肽对肺癌放疗患者急性放射性肺炎及相关细胞因子水平的影响[J].广东医学,2016,37(17):2597-2600.
20 雷章,卢宏达,卢驰,等.还原型谷胱甘肽对大鼠急性放射性肺损伤炎症的影响[J].中国医院药学杂志,2014,34(17):1449-1453.
21 李浩然,李梦娇,刘霏,等.糖代谢中关键酶果糖双磷酸酶-1与卵巢癌化疗敏感性关系的研究[J].中国癌症杂志,2017,27(5):340-344.
2 Li L,Bai H,Yang J,et al.Genome-wide DNA copy number analysis in clonally expanded human ovarian cancer cells with distinct invasive/migratory capacities[J].Oncotarget,2017,8(9):15136-15148.
3 Chang SJ,Bristow RE,Chi DS,et al.Role of aggressive surgical cytoreduction in advanced ovarian cancer[J].J Gynecol Oncol,2015,26(4):336-342.
4 乔志伟,王纯雁,王蕾,等.新辅助化疗联合肿瘤细胞减灭术中热灌注治疗晚期卵巢癌疗效观察[J].湖南师范大学学报(医学版),2015,12(1):83-85.
5 王秀娟,苑中甫,邱海峰,等.术前外周血淋巴细胞/单核细胞比值与上皮性卵巢癌患者预后的关系[J].现代妇产科进展,2016,25(9):654-657.
6 乔红丽,侯炜,李站,等.朴炳奎治疗卵巢癌辨证思路及用药规律总结[J].北京中医药,2014,33(10):735-738.
7 Cheng AY,Zhang Y,Mei HJ,et al.Construction of a plasmid for overexpression of human circadian gene peri-od2and its biological activity in osteosarcoma cells[J].Tumour Biol,2015,36(5):3735-3743.
8 黄景慧,尤冬山,陈欣,等.晚期乳腺癌患者行FAC方案化疗对体内氧化应激水平的影响[J].疑难病杂志,2017,16(4):377-380.
9 杨昆,王学文,赖钊,等.射频消融联合肝动脉化疗栓塞对HCC患者炎性、氧化应激反应及肿瘤活性因子指标影响[J].海南医学院学报,2017,23(4):531-534.
10 Fan XP,Ji XF,Li XY,et al.Methylation of the GlutathioneStransferase P1 gene promoter is associated with oxidative stress in patients with chronic hepatitis B[J].Tohoku J Exp Me,2016,238(1):57-64.
11 柏志强,付熠,黄京璐,等.还原性谷胱甘肽治疗对非ST段抬高型心肌梗死患者近期预后及血浆硫氧还蛋白水平变化的影响[J].热带医学杂志,2015,15(4):507-510.
12 张哲英,张蓓,秦静,等.多器官功能障碍综合征患者血清对内皮细胞氧化损伤的影响及还原型谷胱甘肽的保护作用[J].中国急救医学,2015,35(1):38-43.
13 Razi Soofiyani S,Kazemi T,Lotfipour F,et al.Gene therapy with IL-12induced enhanced anti-tumor activity in fibrosarcoma mouse model[J].Artif Cells Nanomed Biotechnol.2016,44(8):1988-1993.
14 陈立,韩海滨,李佳,等.还原型谷胱甘肽对2型糖尿病早期肾病患者血浆Hcy、尿清蛋白排泄率、甲状腺功能及炎症因子的影响[J].中国中西医结合肾病杂志,2016,17(4):349-351.
15 辛光大,周广宇.还原型谷胱甘肽对老年早期糖尿病肾病患者氧化应激和炎症反应的影响[J].中国老年学杂志,2014,34(6):1449-1450.
16 方芳,赵良中,李强,等.胡桃醌对卵巢癌SKOV3细胞氧化应激和凋亡的影响[J].中国病理生理杂志,2016,32(1):156-159.
17 Qi X,Li Y,Rahe-Meyer N,et al.Intrathecal dexmedetomidine as adjuvant to ropivacaine in hysteroscopic surgery:aprospective,randomized control study[J].Int J Clin Pharmacol Ther,2016,54(3):185-192.
18 柳永兵.还原型谷胱甘肽对尿毒症维持性血液透析患者微炎症状态的影响[J].中国药房,2015,26(12):1637-1639.
19 李超,陈桂明,彭冰,等.还原型谷胱甘肽对肺癌放疗患者急性放射性肺炎及相关细胞因子水平的影响[J].广东医学,2016,37(17):2597-2600.
20 雷章,卢宏达,卢驰,等.还原型谷胱甘肽对大鼠急性放射性肺损伤炎症的影响[J].中国医院药学杂志,2014,34(17):1449-1453.
21 李浩然,李梦娇,刘霏,等.糖代谢中关键酶果糖双磷酸酶-1与卵巢癌化疗敏感性关系的研究[J].中国癌症杂志,2017,27(5):340-344.