摘要
对一株解淀粉芽孢杆菌TF28产生的抗菌脂肽进行分离鉴定及抑菌活性研究,采用酸沉淀、乙酸乙酯和甲醇萃取技术制备了抗菌脂肽粗提物,经过2次HPLC分离纯化,在保留时间32~42min内获得8个抗菌脂肽纯品,经MALDI-TOF-MS鉴定为芬芥素(fengycins),对尖孢镰刀菌和禾谷镰刀菌显示出较强的抑菌活性,该研究为提高菌株TF28抗菌脂肽产量的定向遗传改造奠定基础。
The antifungal lipopeptides produced by Bacillus amyloliquefaciens TF28 have been isolated and identified and their antifungal activity have been studied.The extract of antifungal lipopeptides was prepared by acid precipitation,ethyl acetate and methanol extraction.After two rounds of HPLC separation,eight antifungal lipopeptides were obtained within 32~42 min of retention time,and they have been identified as fengycins by MALDI-TOF-MS,which show strong antifungal activity against Fusarium oxysporum and Fusarium graminearum.These results will contribute to the directional genetic manipulation of the strain TF28 to improve the yield of antifungal lipopeptides.
引文
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