高纯度Nisin的制备及工业生产条件的优化
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摘要
研究了一种制备高纯度Nisin的方法。该方法采用弱酸性离子交换树脂D113对Nisin浓缩液进行静态吸附和动态解吸,然后将解吸液进行盐析、抽滤、烘干、粉碎即得高纯度Nisin。结果表明p H为6,吸附时间为90 min,解吸剂为1 mol/L、V盐酸/V乙醇=1.25的盐酸乙醇溶液,解吸速率为1 BV/h,盐析所用氯化钠浓度为23%为最佳条件,获得的Nisin效价可达34000 IU/mg,收率达到67.45%,具有较好的工业应用前景。
In this study,a new method of preparation high-purity Nisin preparation was researched.The method adopted static adsorption and dynamic desorption of Nisin concentrated solution at a weak acidic ion exchange resin D113.The subsequent production process included salting out,suction filtration,drying and crushing to obtain high-purity Nisin. The results showed that the optimum condition was p H6,adsorption time 90 min,CDesorption agent= 1 mol/L,VHCl/VEt OHl= 1.25,desorption rate1 BV/h,CNa Cl= 23% in salting out.The potency of Nisin attained through this procedure was 34000 IU/mg,total yield reached67.45%.The method has bright prospect in industrial application.
In this study,a new method of preparation high-purity Nisin preparation was researched.The method adopted static adsorption and dynamic desorption of Nisin concentrated solution at a weak acidic ion exchange resin D113.The subsequent production process included salting out,suction filtration,drying and crushing to obtain high-purity Nisin. The results showed that the optimum condition was p H6,adsorption time 90 min,CDesorption agent= 1 mol/L,VHCl/VEt OHl= 1.25,desorption rate1 BV/h,CNa Cl= 23% in salting out.The potency of Nisin attained through this procedure was 34000 IU/mg,total yield reached67.45%.The method has bright prospect in industrial application.
引文
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[2]张玉华,孟一.天然食品防腐剂-nisin的应用[J].食品研究与开发,2003,24(2):109-110.
[3]石小琼,饶华明,张映斌,等.天然食品保鲜防腐剂nisin在豆腐保鲜上的研究应用[J].食品添加剂,2004,11:130-131.
[4]朱露露,农邵庄,李江阔,等.两种生物保鲜液对鲜活海参贮藏品质的影响[J].食品工业,2015,36(6):128-132.
[5]夏云梯,潘立华,朱克美.乳酸链球菌素(Nisin)的特性及在果汁钦料中的应用[J].饮料工业,2000,3(4):14-15.
[6]吴俊强,曹立婷,胡松华.乳酸链球菌素治疗奶牛乳房炎效果观察[C].兽医临床学术会,2008:397-400.
[7]Pachiyappan Kamarajan,Takayuki Hayami,Bibiana Matte,et al.Nisin ZP,a Bacteriocin and Food Preservative,Inhibits Head and Neck Cancer Tumorigenesis and Prolongs Survival[J].PLOS ONE,2015 10(7):e0131008.
[8]Jae M.Shin,Islam Ateia,Jefrey R.Paulus,et al.Antimicrobial nisin acts against saliva derived multi-species biofilms without cytotoxicity to human oral cells[J].Frontiers in Microbiology,2015,6:617.
[9]叶万荣,陈剑,许峰,等.一种高纯度乳酸链球菌素的制备方法:中国,101701034 B[P].2012-06-06.
[10]严宇媛,赵波,赵文杰,等.一种高纯度乳酸链球菌素的制备方法:中国,102199200 B[P].2014-05-07.
[11]刘立毅,宁方红,徐环昕,等.乳酸链球菌素Z纯化脱色小试工艺研究[J].离子交换与吸附,2014,30(5):429-437.
[12]刘立毅,宁方红,徐环昕,等.吸附树脂对发酵上清液中nisin Z的吸附研究[J].中国调味品,2014(39):7-11.
[13]Yuan-Ching Tai,Pranav Joshi,Joseph Mc Guire,et al.Nisin adsorption to hydrophobic surfaces coated with the PEO-PPOPEO triblock surfactant PluronicF108[J].NIH Public Access Author Manuscript,2008 June 1;322(1):112-118.
[14]贾士儒.生物防腐剂[M].中国轻工业出版社,2009:34-35.