3个石榴良种的ISSR分子鉴别
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摘要
为石榴良种的推广栽植和分子标记辅助选择育种奠定理论基础,采用ISSR分子技术方法,从ISSR随机引物中筛选出8条多态性好、谱带清晰、扩增稳定的引物(UBC834、UBC841、UBC811、UBC835、UBC827、UBC861、UBC840、UBC810)对秋艳、青丽和桔艳3个石榴良种进行ISSR-PCR扩增分析鉴别。结果表明:秋艳良种获得的2条特异性分子标记引物为UBC811和UBC834;青丽、桔艳良种获得的2条特异性分子标记引物为UBC835和UBC840,同时获得了3个石榴良种各自的特征图谱,初步建立了分子鉴别体系。
The molecular identification of three good Punica granatum varieties(Qiuyan,Qingli and Juyan)were analyzed by using 8 primers(UBC834,UBC841,UBC811,UBC835,UBC827,UBC861,UBC840 and UBC810)with the advantages of good polymorphism,distinct band and stable amplification selected from ISSR random primers to lay the theoretical foundation for popularization,cultivation and molecular marker-assisted selection of good P.granatumvarieties.Result:Two specific molecular marker primers obtained from Qiuyan variety are UBC811 and UBC834.Two specific molecular marker primers obtained from Qingli and Juyan variety are UBC835 and UBC840.The specific chromatograms of three different P.granatum varieties are obtained. The molecular identification system is established preliminarily.
The molecular identification of three good Punica granatum varieties(Qiuyan,Qingli and Juyan)were analyzed by using 8 primers(UBC834,UBC841,UBC811,UBC835,UBC827,UBC861,UBC840 and UBC810)with the advantages of good polymorphism,distinct band and stable amplification selected from ISSR random primers to lay the theoretical foundation for popularization,cultivation and molecular marker-assisted selection of good P.granatumvarieties.Result:Two specific molecular marker primers obtained from Qiuyan variety are UBC811 and UBC834.Two specific molecular marker primers obtained from Qingli and Juyan variety are UBC835 and UBC840.The specific chromatograms of three different P.granatum varieties are obtained. The molecular identification system is established preliminarily.
引文
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[16]MOSLEMI M,ZAHRAVI M,KHANIKI G B.Genetic diversity and population genetic structure of pomegranate(Punica granatum L.)in Iran using AFLP markers[J].Scientia Horticulturae,2010,126(4):441-447.
[17]SOLEIMANI M H,TALEBI M,SAYED-TABA-TABAEI B E.Use of SRAP markers to assess genetic diversity and population structure of wild,cultivated,and ornamental pomegranates(Punica granatum L.)in different regions of Iran[J].Plant Systematics and Evolution,2012,298(6):1141-1149.
[2]MOHAMMAD H S,MAJID T,BADRALDIN E S.Use of SRAP markers to assess genetic diversity and population structure of wild,cultivated,and ornamental pomegranates(Punica granatum L.)in different regions of Iran[J].Plant Systematics and Evolution,2012,298(6):1141-1149.
[3]郝兆祥,侯乐峰,王艳琴,等.山东省石榴产业可持续发展对策[J].农业科技通讯,2014(6):18-21.
[4]赵丽华,李名扬,王先磊.川滇石榴品种遗传多样性及亲缘关系的AFLP分析[J].林业科学,2010,46(11):168-173.
[5]汪小飞,周耘峰,黄埔,等.石榴品种数量分类研究[J].中国农业科学,2010,43(5):1093-1098.
[6]YUAN Z,YIN Y,QU J,et al.Population Genetic Diversity in Chinese Pomegranate(Punica granatum L.)Cultivars Revealed by Fluorescent-AFLP Markers[J].Journal of Genetics and Genomics,2007,34(12):1061-1071.
[7]王庆军,郝兆祥,罗华,等.分子标记在石榴种质资源遗传多样性研究中的应用[J].山东农业科学,2017,49(9):146-155.
[8]TRIEUA L N,MIENA N T,TIENA T V,et al.Genetic diversity of Panaxstipuleanatus Tsai in North Vietnam detected by inter simple sequence repeat(IS-SR)markers[J].Biotechnology&Biotechnological E-quipment,2016,30(3):506-511.
[9]ZIETKIEWICZ E,RAFALSKI A,LABUDA D.Genome fingerprinting by simple sequence repeat(SSR)-anchored polymerase chain reaction amplification[J].Genomics,1994,20(2):176-259.
[10]HUANG J,GE X,SUN M.Modified CTAB protocol using a silica matrix for isolation of plant genomic DNA[J].Biotechniques,2000,28(3):432-434.
[11]BEKIR D,MUSTAFA C,MURAT U,et al.Study of phylogenetic relationship of Turkish species of Matthiola(Brassicaceae)based on ISSR amplification[J].Turkish Journal of Botany,2016,40(2):130-136.
[12]苑兆和,尹燕雷,朱丽琴,等.山东石榴品种遗传多样性与亲缘关系的荧光AFLP分析[J].园艺学报,2008,35(1):107-112.
[13]陈芸,王继莲,丁晓丽,等.新疆石榴种质资源遗传多样性的SRAP分析[J].西北植物学报,2016,36(5):916-922.
[14]杨荣萍,龙雯虹,张宏,等.云南25份石榴资源的RAPD分析[J].果树学报,2007,24(2):226-229.
[15]JBIR R,HASNAOUI N,MARS M,et al.Characterization of Tunisian pomegranate(Punica granatum L.)cultivars using amplified fragment length polymorphism analysis[J].Scientia Horticulturae,2008,115(3):231-237.
[16]MOSLEMI M,ZAHRAVI M,KHANIKI G B.Genetic diversity and population genetic structure of pomegranate(Punica granatum L.)in Iran using AFLP markers[J].Scientia Horticulturae,2010,126(4):441-447.
[17]SOLEIMANI M H,TALEBI M,SAYED-TABA-TABAEI B E.Use of SRAP markers to assess genetic diversity and population structure of wild,cultivated,and ornamental pomegranates(Punica granatum L.)in different regions of Iran[J].Plant Systematics and Evolution,2012,298(6):1141-1149.