辣椒素对西花蓟马雌成虫体内解毒酶活性的影响
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摘要
【目的】为明确非挥发性次生物质辣椒素对西花蓟马Frankliniella occidentalis雌成虫体内解毒酶活性的影响。【方法】将菜豆叶片研碎离心后取上清液作为西花蓟马的液态饲料,将不同浓度的辣椒素添加到饲料中,饲养西花蓟马雌成虫48、72、96 h后,分别测定西花蓟马体内羧酸酯酶(Car E)、谷胱甘肽-S-转移酶(GSTs)、乙酰胆碱酯酶(AChE)和多功能氧化酶(MFO)的活性。【结果】低剂量辣椒素能诱导西花蓟马雌成虫体内GSTs、AChE和MFO活性明显升高,高剂量辣椒素抑制GSTs、AChE及MFO活性。不论在哪一种辣椒素浓度下,Car E活性一直受到抑制。辣椒素浓度和处理时间对GSTs及AChE活性无明显交互作用,但对Car E及MFO有明显的交互作用,处理时间越长,作用越显著。【结论】西花蓟马雌成虫体内解毒酶活性在次生物质辣椒素处理下发生了明显的变化,说明西花蓟马通过解毒酶适应次生物质对其的影响,但解毒酶的变化有剂量和时间效应。
[Objectives] To investigate the effects of the non-volatile, secondary substance capsaicin on detoxification enzymes in adult female Frankliniella occidentalis. [Methods] Adult female F. occidentalis were fed bean leaf juice to which different concentrations of capsaicin had been added. Detoxification of the enzymes carboxylesterase(Car E), glutathione S-transferase(GSTs),acetylcholine esterase(AChE), and mixed function oxidase(MFO), were determined after F. occidentalis had fed for 48, 72 and 96 h. [Results] Exposure to lower concentrations of capsaicin was followed by a significant increase in GSTs, AChE and MFO activity, but exposure to higher concentrations was followed by a significant decrease in the activity of these enzymes. Car E activity was inhibited by all capsaicin concentrations. No significant interaction between capsaicin concentration and treatment time was apparent for GSTs and AChE, but was for Car E and MFO; the longer the treatment time, the more significant the effect. [Conclusion] The activities of detoxification enzymes in adult female F. occidentalis changed significantly under capsaicin treatment, which indicates that F. occidentalis are able use certain detoxification enzymes to detoxify capsaicin. The magnitude of changes in detoxification enzyme activity were affected by dose and the duration of exposure.
[Objectives] To investigate the effects of the non-volatile, secondary substance capsaicin on detoxification enzymes in adult female Frankliniella occidentalis. [Methods] Adult female F. occidentalis were fed bean leaf juice to which different concentrations of capsaicin had been added. Detoxification of the enzymes carboxylesterase(Car E), glutathione S-transferase(GSTs),acetylcholine esterase(AChE), and mixed function oxidase(MFO), were determined after F. occidentalis had fed for 48, 72 and 96 h. [Results] Exposure to lower concentrations of capsaicin was followed by a significant increase in GSTs, AChE and MFO activity, but exposure to higher concentrations was followed by a significant decrease in the activity of these enzymes. Car E activity was inhibited by all capsaicin concentrations. No significant interaction between capsaicin concentration and treatment time was apparent for GSTs and AChE, but was for Car E and MFO; the longer the treatment time, the more significant the effect. [Conclusion] The activities of detoxification enzymes in adult female F. occidentalis changed significantly under capsaicin treatment, which indicates that F. occidentalis are able use certain detoxification enzymes to detoxify capsaicin. The magnitude of changes in detoxification enzyme activity were affected by dose and the duration of exposure.
引文
Bradford MM,1976.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Anal.Biochem.,72(1):248-254.
Cao Y,Zhi JR,Cong CL,Song QZ,2012.Relationships between host plant selection of Frankliniella occidentalis and physical characteristics and secondary plant compounds of host filiag.Plant Protection,38(4):27-32.[曹宇,郅军锐,从春蕾,宋琼章,2012.西花蓟马寄主选择性与寄主物理性状及次生物质的关系.植物保护,38(4):27-32.]
Chen RF,2011.Studies on Host-adaptation of Frankliniella occidentalis.Master's thesis.Fujian:Fujian Agriculture and Forestry University.[陈锐芬,2011.西花蓟马对寄主植物的适应特性研究.硕士学位论文.福州:福建农林大学.]
Chen CY,Kang ZJ,Shi XY,Gao XW,2015.Metabolic adaptation mechanisms of insects to plant secondary metabolites and their implications for insecticide resistance of insects.Acta Entomologica Sinica,58(10):1126-1139.[陈澄宇,康志娇,史雪岩,高希武,2015.昆虫对植物次生物质的代谢适应机制及其对昆虫抗药性的意义.昆虫学报,58(10):1126-1139.]
Dong JF,Zhang JH,Wang CZ,2002.Effects of plant allelochemicals on nutritional utilization and detoxication enzyme activities in two Helicoverpa species.Acta Entomologica Sinica,45(3):296-300.[董钧锋,张继红,王琛柱,2002.植物次生物质对烟青虫和棉铃虫食物利用及中肠解毒酶活性的影响.昆虫学报,45(3):296-300.]
Hama H,Hosoda A,1983.High aliesterase activity and low acetylcholinesterase sensitivity involved in organophosphorus andcarbamate resistance of the brown planthopper,Nilaparvata lugens Stal(Homoptera:Delphacidae).Applied Entomology and Zoology,18(4):475-485.
Li SX,Zhi JR,Yang GM,Yue WB,Ye M,2016.Resistance of bean leaves induced by exogenous jasmonic acid and its effects on activities of protective and detoxification enzymes in Frankliniella occidentalis.Chinese Journal of Applied Ecology,28(9):2975-2983.[李顺欣,郅军锐,杨广明,岳文波,叶茂,2016.外源茉莉酸诱导的菜豆叶片生化抗性及其对西花蓟马体内保护酶和解毒酶活性的影响.应用生态学报,28(9):2975-2983.]
Liu JN,Huang HP,Hua JZ,Zhang YY,Yao LY,2015.Effects of nicotine on protective and detoxifying enzymes of Phthotimaea opercuella larvae.Chinese Journal of Guizhou Agricultural Sciences,43(3):78-81.[刘佳妮,黄鹤平,华金珠,张瑜瑜,姚丽媛,2015.烟碱对马铃薯块茎蛾幼虫保护酶和解毒酶的影响.贵州农业科学,43(3):78-81.]
Liu RS,Liu Y,Wang J,Lv YF,Bian Y,Zhang LJ,2015.First record of invasive pest Frankliniella occidentalis in Jilin Province.Chinese Journal of Beijing University of Agriculture,30(2):24-26.[刘若思,刘燕,王军,吕玉峰,边勇,张丽杰,2015.重要外来入侵害虫西花蓟马在吉林省部分地区的首次发现.北京农学院学报,30(2):24-26.]
Liu JY,Qian L,Ke R,Chen XY,Li ZY,Gui FR,2017.Effects of elevated carbon dioxide on the activities of physiological enzymes in thrips Frankliniella occidentalis and F.intonsa fed on different host plants.Chinese Journal of Plant Protection,44(1):45-53.[刘建业,钱蕾,可芮,陈晓燕,李正跃,桂富荣,2017.CO2浓度升高对取食不同寄主的西花蓟马和花蓟马生理酶活性的影响.植物保护学报,44(1):45-53.]
Liu SW,Ji MS,2008.Effects of capsaicin on the activities of acetylcholinesterase and carboxylesterase in Plutella xylostella.China Plant Protection,28(4):8-9.[刘少武,纪明山,2008.辣椒碱对小菜蛾体内乙酰胆碱酯酶和羧酸酯酶的影响.中国植保导刊,28(4):8-9.]
Liu X,Lin Y,2003.Biological activity of capsaicine and its joint action with other pesticides.Chinese Journal of Pesticide Science,5(2):94-96.[刘新,林永,2003.辣椒碱对桃蚜的生物活性及其与几种杀虫剂的联合作用.农药学学报,5(2):94-96.]
Lv M,Sun HH,Wang LH,Gao XW,2012.Effects of secondary metabolites on activities of glutathione s-transferases,carboxylesterase in aphid.Chinese Agricultural Science Bulletin,28(3):253-256.[吕敏,孙婳婳,王丽红,高希武,2012.植物次生物质对棉蚜谷胱甘肽S-转移酶和羧酸酯酶活性的诱导作用.中国农学通报,28(3):253-256.]
Ma ZQ,Li GZ,Feng JT,Zhang X,2007.Effects of deoxypodophyllotoxin on metabolizing enzymes in Mythimna separata Walker.Acta Entomologica Sinica,50(2):186-190.[马志卿,李广泽,冯俊涛,张兴,2007.脱氧鬼臼毒素对粘虫几种代谢酶系的影响.昆虫学报,50(2):186-190.]
Mittler TE,Dadd RH,1964.An improved method for feeding aphids on artificial diets.Annals of the Entomological Society of America,57:139-140.
Pei CY,Zheng CY,2011.Selectivity of Frankliniella occidentalis(Pergande)on different host vegetables.Chinese Journal of Eco-Agriculture,19(2):383-387.[裴昌莹,郑长英,2011.西花蓟马对不同蔬菜寄主的选择性研究.中国农业生态学报,19(2):383-387.]
Pan YF,Meng JY,Zhang XY,Zhou XM,Lei CL,2006.Effects of host plants on insecticide susceptibility of bean pod borer,Maruca testulalis,and activity of its detoxification enzyme.Chinese Journal of Applied Entomology,43(4):496-500.[潘亚飞,孟建玉,张小亚,周兴苗,雷朝亮,2006.寄主对豆野螟的药剂敏感性和体内解毒酶活性的影响.应用昆虫学报,43(4):496-500.]
Qian Y,Cao GC,Song JX,Yin Q,Han ZJ,2008.Biochemical mechanisms conferring cross-resistance between tebufeno-zide and abamectin in Plutella xylostella.Pesticide Biochemistry and Physiology,91(3):175-179.
Wang RL,Sun YL,Liang XT,Song YY,Su YJ,Zhu KY,Zeng RS,2012.Effects of six plant secondary metabolites on activities of detoxification enzymes in Spodoptera litura.Acta Ecologica Sinica,32(16):5191-5198.[王瑞龙,孙玉林,梁笑婷,宋圆圆,苏贻娟,朱克岩,曾任森,2012.6种植物次生物质对斜纹夜蛾解毒酶活性的影响.生态学报,32(16):5191-5198.]
Wang XY,Huang BQ,1999.Effects of tea saponin on several enzymes of the imported cabbage worm pieris rapae L..Chinese Journal of Tea,25(4):193-196.[王小艺,黄炳球,1999.茶皂素对菜青虫体内几种酶活性的影响.茶叶,25(4):193-196.]
Wu DD,Zhao JW,Chen Y,Zheng Y,He YX,2012.Effects of capsaicin on the activities of carboxylesterase,glutathione S-transferase and acetylcholinesterase in Bemisia tabaci.Chinese Journal of Biosafety,21(2):109-113.[吴咚咚,赵建伟,陈颖,郑宇,何玉仙,2012.辣椒碱对烟粉虱体内羧酸酯酶、谷胱甘肽-S-转移酶和乙酰胆碱酯酶活性的影响.生物安全学报,21(2):109-113.]
Wu G,2009.Effects of plant insect-resistant compounds on the nutritional utilization and enzyme activities of beet armyworm,Spodoptera exigua(Hübner).Doctoral dissertation.Beijing:Chinese Academy of Agricultural Sciences.[吴刚,2009.植物抗虫物质对甜菜夜蛾营养效应和酶活性的影响.博士学位论文.北京:中国农业科学院.]
Zhang ZK,Wu SY,Lei ZR,Kang PZ,Du YI,Zhang LR,2017.Development and reproduction of Frankliniella occidentalis(Pergande)in greenhouse pepper in Ningxia.Chinese Journal of China Plant Protection,37(7):35-38.[张治科,吴圣勇,雷仲仁,康萍芝,杜玉宁,张丽荣,2017.宁夏设施辣椒上西花蓟马的生长发育和繁殖研究.中国植保导刊,37(7):35-38.]
Zhi JR,Tian T,Wen J,Liu Y,2016.Effects of kidney bean damaged by Frankliniella occidentalis(Thysanoptera:Thripidae)or Tetranychus urticae(Acari:Tetranychidae)on the activities of protective and detoxification enzymes in the other subsequent herbivoreof both.Acta Entomologica Sinica,59(7):707-715.[郅军锐,田甜,温娟,刘勇,2016.西花蓟马或二斑叶螨为害的菜豆对两者间后取食者体内保护酶和解毒酶活性的影响.昆虫学报,59(7):707-715.]
Zhou L,2011.Toxicity of total alkaloid from Tripterygium wilfordii Hook against Mythimna separata(Walker)and its effects of on ACh E and Ach.Acta Agriculture Boreali-Sinica,26(1):215-218.[周琳,2011.雷公藤总生物碱对粘虫幼虫的毒力及对乙酰胆碱酯酶和乙酰胆碱的影响.华北农学报,26(1):215-218.]
Zhou Z,Cheng XS,Wang FX,Chen SR,2007.Effects of nicotine and rutin on the susceptibility of Spodoptera litura to insecticides and the activities of some enzymes.Chinese Journal of Pesticide Science,9(3):305-308.[周郑,程新胜,王方晓,陈树仁,2007.烟碱和芸香苷对斜纹夜蛾药剂敏感性及相关酶活性的影响.农药学学报,9(3):305-308.]
Cao Y,Zhi JR,Cong CL,Song QZ,2012.Relationships between host plant selection of Frankliniella occidentalis and physical characteristics and secondary plant compounds of host filiag.Plant Protection,38(4):27-32.[曹宇,郅军锐,从春蕾,宋琼章,2012.西花蓟马寄主选择性与寄主物理性状及次生物质的关系.植物保护,38(4):27-32.]
Chen RF,2011.Studies on Host-adaptation of Frankliniella occidentalis.Master's thesis.Fujian:Fujian Agriculture and Forestry University.[陈锐芬,2011.西花蓟马对寄主植物的适应特性研究.硕士学位论文.福州:福建农林大学.]
Chen CY,Kang ZJ,Shi XY,Gao XW,2015.Metabolic adaptation mechanisms of insects to plant secondary metabolites and their implications for insecticide resistance of insects.Acta Entomologica Sinica,58(10):1126-1139.[陈澄宇,康志娇,史雪岩,高希武,2015.昆虫对植物次生物质的代谢适应机制及其对昆虫抗药性的意义.昆虫学报,58(10):1126-1139.]
Dong JF,Zhang JH,Wang CZ,2002.Effects of plant allelochemicals on nutritional utilization and detoxication enzyme activities in two Helicoverpa species.Acta Entomologica Sinica,45(3):296-300.[董钧锋,张继红,王琛柱,2002.植物次生物质对烟青虫和棉铃虫食物利用及中肠解毒酶活性的影响.昆虫学报,45(3):296-300.]
Hama H,Hosoda A,1983.High aliesterase activity and low acetylcholinesterase sensitivity involved in organophosphorus andcarbamate resistance of the brown planthopper,Nilaparvata lugens Stal(Homoptera:Delphacidae).Applied Entomology and Zoology,18(4):475-485.
Li SX,Zhi JR,Yang GM,Yue WB,Ye M,2016.Resistance of bean leaves induced by exogenous jasmonic acid and its effects on activities of protective and detoxification enzymes in Frankliniella occidentalis.Chinese Journal of Applied Ecology,28(9):2975-2983.[李顺欣,郅军锐,杨广明,岳文波,叶茂,2016.外源茉莉酸诱导的菜豆叶片生化抗性及其对西花蓟马体内保护酶和解毒酶活性的影响.应用生态学报,28(9):2975-2983.]
Liu JN,Huang HP,Hua JZ,Zhang YY,Yao LY,2015.Effects of nicotine on protective and detoxifying enzymes of Phthotimaea opercuella larvae.Chinese Journal of Guizhou Agricultural Sciences,43(3):78-81.[刘佳妮,黄鹤平,华金珠,张瑜瑜,姚丽媛,2015.烟碱对马铃薯块茎蛾幼虫保护酶和解毒酶的影响.贵州农业科学,43(3):78-81.]
Liu RS,Liu Y,Wang J,Lv YF,Bian Y,Zhang LJ,2015.First record of invasive pest Frankliniella occidentalis in Jilin Province.Chinese Journal of Beijing University of Agriculture,30(2):24-26.[刘若思,刘燕,王军,吕玉峰,边勇,张丽杰,2015.重要外来入侵害虫西花蓟马在吉林省部分地区的首次发现.北京农学院学报,30(2):24-26.]
Liu JY,Qian L,Ke R,Chen XY,Li ZY,Gui FR,2017.Effects of elevated carbon dioxide on the activities of physiological enzymes in thrips Frankliniella occidentalis and F.intonsa fed on different host plants.Chinese Journal of Plant Protection,44(1):45-53.[刘建业,钱蕾,可芮,陈晓燕,李正跃,桂富荣,2017.CO2浓度升高对取食不同寄主的西花蓟马和花蓟马生理酶活性的影响.植物保护学报,44(1):45-53.]
Liu SW,Ji MS,2008.Effects of capsaicin on the activities of acetylcholinesterase and carboxylesterase in Plutella xylostella.China Plant Protection,28(4):8-9.[刘少武,纪明山,2008.辣椒碱对小菜蛾体内乙酰胆碱酯酶和羧酸酯酶的影响.中国植保导刊,28(4):8-9.]
Liu X,Lin Y,2003.Biological activity of capsaicine and its joint action with other pesticides.Chinese Journal of Pesticide Science,5(2):94-96.[刘新,林永,2003.辣椒碱对桃蚜的生物活性及其与几种杀虫剂的联合作用.农药学学报,5(2):94-96.]
Lv M,Sun HH,Wang LH,Gao XW,2012.Effects of secondary metabolites on activities of glutathione s-transferases,carboxylesterase in aphid.Chinese Agricultural Science Bulletin,28(3):253-256.[吕敏,孙婳婳,王丽红,高希武,2012.植物次生物质对棉蚜谷胱甘肽S-转移酶和羧酸酯酶活性的诱导作用.中国农学通报,28(3):253-256.]
Ma ZQ,Li GZ,Feng JT,Zhang X,2007.Effects of deoxypodophyllotoxin on metabolizing enzymes in Mythimna separata Walker.Acta Entomologica Sinica,50(2):186-190.[马志卿,李广泽,冯俊涛,张兴,2007.脱氧鬼臼毒素对粘虫几种代谢酶系的影响.昆虫学报,50(2):186-190.]
Mittler TE,Dadd RH,1964.An improved method for feeding aphids on artificial diets.Annals of the Entomological Society of America,57:139-140.
Pei CY,Zheng CY,2011.Selectivity of Frankliniella occidentalis(Pergande)on different host vegetables.Chinese Journal of Eco-Agriculture,19(2):383-387.[裴昌莹,郑长英,2011.西花蓟马对不同蔬菜寄主的选择性研究.中国农业生态学报,19(2):383-387.]
Pan YF,Meng JY,Zhang XY,Zhou XM,Lei CL,2006.Effects of host plants on insecticide susceptibility of bean pod borer,Maruca testulalis,and activity of its detoxification enzyme.Chinese Journal of Applied Entomology,43(4):496-500.[潘亚飞,孟建玉,张小亚,周兴苗,雷朝亮,2006.寄主对豆野螟的药剂敏感性和体内解毒酶活性的影响.应用昆虫学报,43(4):496-500.]
Qian Y,Cao GC,Song JX,Yin Q,Han ZJ,2008.Biochemical mechanisms conferring cross-resistance between tebufeno-zide and abamectin in Plutella xylostella.Pesticide Biochemistry and Physiology,91(3):175-179.
Wang RL,Sun YL,Liang XT,Song YY,Su YJ,Zhu KY,Zeng RS,2012.Effects of six plant secondary metabolites on activities of detoxification enzymes in Spodoptera litura.Acta Ecologica Sinica,32(16):5191-5198.[王瑞龙,孙玉林,梁笑婷,宋圆圆,苏贻娟,朱克岩,曾任森,2012.6种植物次生物质对斜纹夜蛾解毒酶活性的影响.生态学报,32(16):5191-5198.]
Wang XY,Huang BQ,1999.Effects of tea saponin on several enzymes of the imported cabbage worm pieris rapae L..Chinese Journal of Tea,25(4):193-196.[王小艺,黄炳球,1999.茶皂素对菜青虫体内几种酶活性的影响.茶叶,25(4):193-196.]
Wu DD,Zhao JW,Chen Y,Zheng Y,He YX,2012.Effects of capsaicin on the activities of carboxylesterase,glutathione S-transferase and acetylcholinesterase in Bemisia tabaci.Chinese Journal of Biosafety,21(2):109-113.[吴咚咚,赵建伟,陈颖,郑宇,何玉仙,2012.辣椒碱对烟粉虱体内羧酸酯酶、谷胱甘肽-S-转移酶和乙酰胆碱酯酶活性的影响.生物安全学报,21(2):109-113.]
Wu G,2009.Effects of plant insect-resistant compounds on the nutritional utilization and enzyme activities of beet armyworm,Spodoptera exigua(Hübner).Doctoral dissertation.Beijing:Chinese Academy of Agricultural Sciences.[吴刚,2009.植物抗虫物质对甜菜夜蛾营养效应和酶活性的影响.博士学位论文.北京:中国农业科学院.]
Zhang ZK,Wu SY,Lei ZR,Kang PZ,Du YI,Zhang LR,2017.Development and reproduction of Frankliniella occidentalis(Pergande)in greenhouse pepper in Ningxia.Chinese Journal of China Plant Protection,37(7):35-38.[张治科,吴圣勇,雷仲仁,康萍芝,杜玉宁,张丽荣,2017.宁夏设施辣椒上西花蓟马的生长发育和繁殖研究.中国植保导刊,37(7):35-38.]
Zhi JR,Tian T,Wen J,Liu Y,2016.Effects of kidney bean damaged by Frankliniella occidentalis(Thysanoptera:Thripidae)or Tetranychus urticae(Acari:Tetranychidae)on the activities of protective and detoxification enzymes in the other subsequent herbivoreof both.Acta Entomologica Sinica,59(7):707-715.[郅军锐,田甜,温娟,刘勇,2016.西花蓟马或二斑叶螨为害的菜豆对两者间后取食者体内保护酶和解毒酶活性的影响.昆虫学报,59(7):707-715.]
Zhou L,2011.Toxicity of total alkaloid from Tripterygium wilfordii Hook against Mythimna separata(Walker)and its effects of on ACh E and Ach.Acta Agriculture Boreali-Sinica,26(1):215-218.[周琳,2011.雷公藤总生物碱对粘虫幼虫的毒力及对乙酰胆碱酯酶和乙酰胆碱的影响.华北农学报,26(1):215-218.]
Zhou Z,Cheng XS,Wang FX,Chen SR,2007.Effects of nicotine and rutin on the susceptibility of Spodoptera litura to insecticides and the activities of some enzymes.Chinese Journal of Pesticide Science,9(3):305-308.[周郑,程新胜,王方晓,陈树仁,2007.烟碱和芸香苷对斜纹夜蛾药剂敏感性及相关酶活性的影响.农药学学报,9(3):305-308.]