柑橘铜胁迫研究进展
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摘要
铜是植物必需的矿质营养元素,在柑橘生长发育过程中起着重要作用。国内外长期广泛应用铜制杀菌剂防治溃疡病、炭疽病等,致使果园土壤积累了过量铜,甚至部分柑橘园树体出现铜中毒现象。另一方面,我国柑橘产区背景土壤有效铜含量较低,不使用或很少使用铜制剂的果园树体又易出现缺铜现象。铜胁迫会影响柑橘树体正常生理代谢,导致果实品质、产量下降。就此,笔者对柑橘铜胁迫现状、铜胁迫对柑橘生长发育和新陈代谢的影响、铜胁迫抗性机制等进行了综述,并对柑橘铜胁迫的影响因子、柑橘砧木对铜胁迫的耐性差异以及铜胁迫防治措施进行了概括,旨在为铜胁迫有关研究以及生产实践提供参考。
Copper(Cu) is an element necessary for citrus, which plays an important role in the process of citrus growth and development. Copper fungicides have been widely used to control citrus canker and anthracnose since the 19 th century, resulting in accumulation of copper in soils of citrus orchards.Otherwise, the available copper content of virgin soil in the citrus production areas of China is relatively low. Leaf nutrient of citrus was influenced by soil nutrient status. Copper deficiency and excess would cause stresses in citrus orchards. This paper summarized the effects of copper stress on citrus growth and metabolism, the factors effecting copper stress, and the tolerance of citrus rootstocks to copper stress. The research progress of heavy metal stress-associated proteins was also introduced. Copper participates in several physiological processes of plants. Cu-deficient plants exhibit a reduction in electron transport during photosynthesis due to decreases of plastocyanin synthesis and the contents of chlorophylls and carotenoids. Copper as a cofactor of enzymes, has an influence on the activity of antioxidant enzyme that scavenge reactive oxygen species(ROS). Copper deficiency affects the lignification process by decreasing lignin, hemicellulose and acetyl contents of cell walls. The uptake of zinc is inhibited in copper-deficient plants. Citrus orchards with high organic matter content, high p H, and rarely use of copper fungicides, are more likely to have Cu-deficient. Visual symptoms of deficiency are characterized by curved or"S-shaped"branches with large, dark green and overdeveloped leaves. In severe cases, reddish brown droplets of gum cover the twigs. As the deficiency becomes acute, the twigs start to die. Copper toxicity in plants affects physiological and biochemical processes, resulting in the inhibition of shoot and root growth. Leaf chlorosis, black and shorter roots have been observed on copper toxicity plants. The excess Cu decreases the concentration of chlorophyll, which results in a reduction in the en-ergy transfer from the antennae of the PSII to the reaction centers. And net photosynthetic rate is significantly reduced. Furthermore, excess copper reduces the ability of the plant to explore the soil for water and essential nutrients. It has been reported that high copper inhibits the uptake and activity of Zn2 +,Fe2 +, Mg2 +, et al. An excess of Cu also leads to serious damage to the root and leaves ultra-structure of citrus. The membrane system of chloroplast and mitochondria is damaged greatly. A thin and twisted cell wall, degeneration of the middle lamella, cellular plasmolysis can be observed in the root cell of copper toxicity plants. Many researches revealed that high copper stresses would result in oxidative stress due to enhanced ROS production. In order to scavenge ROS and alleviate their deleterious effects in cells, plants have increased activities of SOD, CAT and peroxidases(APX and POX). The availability and mobility of Cu in the soils are related to organic matter content and soil p H. High organic matter content and high p H could reduce the availability of copper in soil. It can effectively alleviate the copper toxicity of citrus when soil p H was 6.5 or over 6.5. The application of fertilizer can also affect the uptake of copper by citrus. Nitrogen(N) fertilizer applied in the NH4+form would result in some degree of soil acidification, which could enhance the leaching of nutrients in sandy soil. Excessive applications of N and P fertilizers in citrus orchards would cause copper deficiency. Previous study showed that increased Ca availability in the rooting environment would ameliorate the effects of Cu phytotoxicity.And sufficient P supply has positive effect on citrus seedling under excess copper. The interaction of rootstock with Cu toxicity in citrus trees has been evaluated in some researches. Results showed that,‘Swingle citrumelo'rootstock might have better tolerance than‘Sunki mandarin'and‘Rangpur lime'.And rough lemon is more tolerant to Cu phytotoxicity than‘Cleopatra mandarin'. Plants have developed various mechanisms, including avoidance of uptake, chelation, efflux, autoxidation, and intracellular sequestration, to overcome Cu toxicity. The storage of Cu in the root cell walls and vacuole may keep the ion sequestered from the metabolic center. The conversion of Cu2 +to no-toxic complex compound by protein, organic acid or phenolic compounds, is one of the important detoxification mechanisms. The improvement of antioxidant system is also a manifestation of alleviating Cu stress. Heavy metal binding proteins and transporters play important roles in resistance to copper stress. Various protein families related to Cu uptake and transportation were found in plants. Cs MT1 was identified in sweet orange, and mainly expressed in leaves and flowers. COPT gene family encode Cu transporter with high affinity, and 6 COPT family members has been found in Arabidopsis. Researches indicated HMA1 and HMA5-HMA8 were related to Cu transport between organelles. Recently, 12 MTP genes were identified in sweet orange, named as Cit MTP1 and Cit MTP3 to Cit MTP12 based on their sequence similarity to Arabidopsis thaliana MTPs.
Copper(Cu) is an element necessary for citrus, which plays an important role in the process of citrus growth and development. Copper fungicides have been widely used to control citrus canker and anthracnose since the 19 th century, resulting in accumulation of copper in soils of citrus orchards.Otherwise, the available copper content of virgin soil in the citrus production areas of China is relatively low. Leaf nutrient of citrus was influenced by soil nutrient status. Copper deficiency and excess would cause stresses in citrus orchards. This paper summarized the effects of copper stress on citrus growth and metabolism, the factors effecting copper stress, and the tolerance of citrus rootstocks to copper stress. The research progress of heavy metal stress-associated proteins was also introduced. Copper participates in several physiological processes of plants. Cu-deficient plants exhibit a reduction in electron transport during photosynthesis due to decreases of plastocyanin synthesis and the contents of chlorophylls and carotenoids. Copper as a cofactor of enzymes, has an influence on the activity of antioxidant enzyme that scavenge reactive oxygen species(ROS). Copper deficiency affects the lignification process by decreasing lignin, hemicellulose and acetyl contents of cell walls. The uptake of zinc is inhibited in copper-deficient plants. Citrus orchards with high organic matter content, high p H, and rarely use of copper fungicides, are more likely to have Cu-deficient. Visual symptoms of deficiency are characterized by curved or"S-shaped"branches with large, dark green and overdeveloped leaves. In severe cases, reddish brown droplets of gum cover the twigs. As the deficiency becomes acute, the twigs start to die. Copper toxicity in plants affects physiological and biochemical processes, resulting in the inhibition of shoot and root growth. Leaf chlorosis, black and shorter roots have been observed on copper toxicity plants. The excess Cu decreases the concentration of chlorophyll, which results in a reduction in the en-ergy transfer from the antennae of the PSII to the reaction centers. And net photosynthetic rate is significantly reduced. Furthermore, excess copper reduces the ability of the plant to explore the soil for water and essential nutrients. It has been reported that high copper inhibits the uptake and activity of Zn2 +,Fe2 +, Mg2 +, et al. An excess of Cu also leads to serious damage to the root and leaves ultra-structure of citrus. The membrane system of chloroplast and mitochondria is damaged greatly. A thin and twisted cell wall, degeneration of the middle lamella, cellular plasmolysis can be observed in the root cell of copper toxicity plants. Many researches revealed that high copper stresses would result in oxidative stress due to enhanced ROS production. In order to scavenge ROS and alleviate their deleterious effects in cells, plants have increased activities of SOD, CAT and peroxidases(APX and POX). The availability and mobility of Cu in the soils are related to organic matter content and soil p H. High organic matter content and high p H could reduce the availability of copper in soil. It can effectively alleviate the copper toxicity of citrus when soil p H was 6.5 or over 6.5. The application of fertilizer can also affect the uptake of copper by citrus. Nitrogen(N) fertilizer applied in the NH4+form would result in some degree of soil acidification, which could enhance the leaching of nutrients in sandy soil. Excessive applications of N and P fertilizers in citrus orchards would cause copper deficiency. Previous study showed that increased Ca availability in the rooting environment would ameliorate the effects of Cu phytotoxicity.And sufficient P supply has positive effect on citrus seedling under excess copper. The interaction of rootstock with Cu toxicity in citrus trees has been evaluated in some researches. Results showed that,‘Swingle citrumelo'rootstock might have better tolerance than‘Sunki mandarin'and‘Rangpur lime'.And rough lemon is more tolerant to Cu phytotoxicity than‘Cleopatra mandarin'. Plants have developed various mechanisms, including avoidance of uptake, chelation, efflux, autoxidation, and intracellular sequestration, to overcome Cu toxicity. The storage of Cu in the root cell walls and vacuole may keep the ion sequestered from the metabolic center. The conversion of Cu2 +to no-toxic complex compound by protein, organic acid or phenolic compounds, is one of the important detoxification mechanisms. The improvement of antioxidant system is also a manifestation of alleviating Cu stress. Heavy metal binding proteins and transporters play important roles in resistance to copper stress. Various protein families related to Cu uptake and transportation were found in plants. Cs MT1 was identified in sweet orange, and mainly expressed in leaves and flowers. COPT gene family encode Cu transporter with high affinity, and 6 COPT family members has been found in Arabidopsis. Researches indicated HMA1 and HMA5-HMA8 were related to Cu transport between organelles. Recently, 12 MTP genes were identified in sweet orange, named as Cit MTP1 and Cit MTP3 to Cit MTP12 based on their sequence similarity to Arabidopsis thaliana MTPs.
引文
[1]ILYAS A,ASHRAF M Y,HUSSAIN M,ASHRAF M,AHMED R,KAMAL A.Effect of micronutrients(Zn,Cu and B)on photosynthetic and fruit yield attributes of Citrus reticulata Blanco var.kinnow[J].Pakistan Journal of Botany,2015,47(4):1241-1247.
[2]庄伊美.柑桔营养与施肥[M].北京:中国农业出版社,1994:42-43.ZHUANG Yimei.The nutrition and fertilization of Citrus[M].Beijing:China Agricultural Press,1994:42-43.
[3]唐玉琴,彭良志,淳长品,凌丽俐,方贻文,严翔.红壤甜橙园土壤和叶片营养元素相关性分析[J].园艺学报,2013,40(4):623-632.TANG Yuqin,PENG Liangzhi,CHUN Changpin,LING Lili,FANG Yiwen,YAN Xiang.Correlation analysis on nutrient element contents in orchard soils and sweet orange leaves in southern Jiangxi province of China[J].Acta Horticulturae Scinca,2013,40(4):623-632.
[4]陶梦铭,应虹,孙强,谭启玲,李翠,胡承孝.湖北与湖南柑橘园土壤及树体养分状况[J].湖北农业科学,2016,55(13):3289-3292.TAO Mengming,YING Hong,SUN Qiang,TAN Qiling,LI Cui,HU Chengxiao.Soil and plant nutrient status of citrus orchard in Hubei and Hunan province[J].Hubei Agricultural Sciences,2016,55(13):3289-3292.
[5]刘铮,朱其清,唐丽华,徐俊祥,尹楚良.我国缺乏微量元素的土壤及其区域分布[J].土壤学报,1982,19(3):209-223.LIU Zheng,ZHU Qiqing,TANG Lihua,XU Junxiang,YIN Chuliang.Geographical distribution of trace elements-deficient soils in China[J].Acta Pedologica Sinica,1982,19(3):209-223.
[6]曹立,彭良志,淳长品,凌丽俐,赖九江,闵嗣璠,江才伦.赣南不同土壤类型脐橙园叶片营养状况研究[J].中国南方果树,2012,41(2):5-9.CAO Li,PENG Liangzhi,CHUN Changpin,LING Lili,LAI Jiujiang,MIN Sifan,JIANG Cailun.Study on leaf nutrition of Newhall Orange on different soil types in southern Jiangxi[J].South China Fruits,2012,41(2):5-9.
[7]曾艳,黄金生,周柳强,谢如林,刘永贤,谭裕模,谭宏伟.广西桂南蔗区土壤养分状况调查分析[J].南方农业学报,2014,45(12):2198-2202.ZENG Yan,HUANG Jinsheng,ZHOU Liuqiang,XIE Rulin,LIU Yongxian,TAN Yumo,TAN Hongwei.Investigation of soil nutrient status in sugarcane-growing area of south Guangxi[J].Journal of Southern Agriculture,2014,45(12):2198-2202.
[8]杨夫臣,涂俊凡,秦仲麒,李先明,朱红艳,伍涛.湖北省砂梨主产区梨园土壤营养状况评价[J].农业科技通讯,2014(6):97-100.YANG Fucheng,TU Junfan,QIN Zhonglin,LI Xianming,ZHU Hongyan,WU Tao.Study on soil nutrient status in pear-growing area of Hubei[J].Bulletin of Agricultural Science and Technology,2014(6):97-100.
[9]俄胜哲,袁继超,丁志勇,姚凤娟,喻小平,罗付香.氮磷钾肥对稻米铁、锌、铜、锰、镁、钙含量和产量的影响[J].中国水稻科学,2005,19(5):434-440.E Shengzhe,YUAN Jichao,DING Zhiyong,YAO Fengjuan,YU Xiaoping,LUO Fuxiang.Effect of N,P,K fertilizers on Fe,Zn,Cu,Mn,Ca and Mg contents and yields in rice[J].Chinese Journal of Rice Science,2005,19(5):434-440.
[10]TIMMER L W,LEYDEN R F.The relationship of mycorrhizal infection to phosphorus-induced copper deficiency in sour orange seedlings[J].New Phytologist,1980,85(1):15-23.
[11]LIMA J S.Copper balances in cocoa agrarian ecosystems:effects of differential use of cupric fungicides[J].Agriculture,Ecosystems and Environment,1994,48(1):19-25.
[12]FAN J H,HE Z L,LENA Q M,STOFFELLA P J.Accumulation and availability of copper in citrus grove soils as affected by fungicide application[J].Journal of Soils and Sediments,2011,11(4):639-648.
[13]徐秋桐,张莉,章明奎.长期喷施波尔多液对葡萄园土壤、树体和径流中铜积累的影响[J].水土保持学报,2014,28(2):195-198.XU Qiutong,ZHANG Li,ZHANG Mingkui.Accumulation of copper in soil,plant tissue,amd runoff in vineyard as affected by long-term application of Bordeax mixture[J].Journal of Soil and Water Conservation,2014,28(2):195-198.
[14]潘琼,程运林.土壤重金属污染现状调查与评价[J].环境与可持续发展,2013,38(6):47-49.PAN Qiong,CHENG Yunlin.Soil heavy metal pollution present situation investigation and evaluation[J].Environment and Sustainable Development,2013,38(6):47-49.
[15]卜元卿,石利利,单正军.波尔多液在苹果和土壤中残留动态及环境风险评价[J].农业环境科学学报,2013,32(5):972-978.BU Yuanqing,SHI Lili,SHAN Zhengjun.Residue dynamic and environmental risk assessment of Bordeaux mixture in apple and soil[J].Journal of Agro-Environment Science,2013,32(5):972-978.
[16]张旭,曲燕,梁东丽,何洁,孙鑫.全国主要土壤铜、锌、镍重金属形态及其与土壤性质的关系[J].环境科学导刊,2016,35(5):9-14.ZHANG Xu,QU Yan,LIANG Dongli,HE Jie,SUN Xin.Primary study on the relationships between heavy metal speciation and their soil properties in China[J].Environmental Science Survey,2016,35(5):9-14.
[17]王秀丽,徐建民,姚槐应,谢正苗.重金属铜、锌、镉、铅复合污染对土壤环境微生物群落的影响[J].环境科学学报,2003,23(1):22-27.WANG Xiuli,XU Jianmin,YAO Huaiying,XIE Zhengmiao.Effects of Cu,Zn,Cd and Pb compound contamination on soil microbial community[J].Acta Scientiae Circumstantiae,2003,23(1):22-27.
[18]王男麒,彭良志,淳长品,李勋,薛珺,范玉兰,梁梅青.赣南柑桔园背景土壤营养状况分析[J].中国南方果树,2012,41(5):1-4.WANG Nanqi,PENG Liangzhi,CHUN Changpin,LI Xun,XUE Jun,FAN Yulan,LIANG Meiqing.Study on virgin soil nutrient status of citrus orchards in southern Jiangxi province[J].South China Fruits,2012,41(5):1-4.
[19]范西宁,黄玉溢,刘斌,陈桂芬.广西红壤柑桔园土壤剖面有效微量元素含量研究[J].广西农业科学,2007,38(3):285-287.FAN Xining,HUANG Yuyi,LIU Bin,CHEN Guifen.Contents of available microelements in soil profiles of citrus orchards with red soil in Guangxi[J].Guangxi Agricultural Sciences,2007,38(3):285-287.
[20]余红兵,王仁才,肖润林,杨知建.桂西北环境移民示范区柑橘园土壤和叶片营养状况[J].湖南农业大学学报(自然科学版),2007,33(3):341-347.YU Hongbing,WANG Rencai,XIAO Runlin,YANG Zhijian.Soil and leaf nutrient condition of plants from Citrus orchards in demonstration area of environmental immigrants,northwest Guangxi province[J].Journal of Hunan Agricultural University(Natural Sciences),2007,33(3):341-347.
[21]范玉兰,卢映琼,李峰,薛珺,李勋,钟八莲,彭良志.赣南脐橙园土壤有效铜含量分布特征研究[J].果树学报,2015,32(1):69-73.FAN Yulan,LU Yingqiong,LI Feng,XUE Jun,LI Xun,ZHONG Balian,PENG Liangzhi.Study on distribution of available copper content in soil of navel orange orchards in southern Jiangxi province[J].Journal of Fruit Science,2015,32(1):69-73.
[22]周鑫斌,石孝均,孙彭寿,李伟,戴亨林,彭良志,淳长品.三峡重庆库区柑橘园土壤养分丰缺状况研究[J].植物营养与肥料学报,2010,16(4):817-823.ZHOU Xinbin,SHI Xiaojun,SUN Pengshou,LI Wei,DAI Henglin,PENG Liangzhi,CHUN Changpin.Status of soil fertility in citrus orchards of Chongqing Sanxia Reservoir Area[J].Plant Nutrition and Fertilizer,2010,16(4):817-823.
[23]习建龙,彭良志,淳长品,朱春钊,杨惠栋,王男麒,江才伦,凌丽俐.重庆三峡库区加工甜橙园土壤养分状况分析[J].中国南方果树,2015,44(1):1-7.XI Jianlong,PENG Liangzhi,CHUN Changpin,ZHU Chunzhao,YANG Huidong,WANG Nanqi,JIANG Cailun,LING Lili.Analysis of nutriention status of the soil in processing orange orchards in Three Gorges Reservoir Region of Chongqing[J].South China Fruits,2015,44(1):1-7.
[24]鲁剑巍,陈防,王富华,刘冬碧,万运帆,余常兵,胡芳林,王明锐,王运华.湖北省柑橘园土壤养分分级研究[J].植物营养与肥料学报,2002,8(4):390-394.LU Jianwei,CHEN Fang,WANG Fuhua,LIU Dongbi,WAN Yunfan,YU Changbing,HU Fanglin,WANG Mingrui,WANG Yunhua.Study of classification of the soil nutrient status of citrus orchard in Hubei province[J].Plant Nutrition and Fertilizer Science,2002,8(4):390-394.
[25]应介官,刘秀红,李江波,吴群,彭抒昂,姜存仓.江西南丰和浙江衢州柑桔园土壤及叶片铜、锰营养状况调查[J].中国南方果树,2016,45(6):15-18.YING Jieguan,LIU Xiuhong,LI Jiangbo,WU Qun,PENG Shu’ang,JIANG Cuncang.Analysis of Cu and Mn contents in the soil and leaves of citrus orchards in Nanfeng and Quzhou[J].South China Fruits,2016,45(6):15-18.
[26]林媚,温明霞,冯先桔,张伟清,姚周麟,平新亮.台州柑橘园土壤有效铜锌养分现状[J].浙江农业科学,2012,1(3):321-323.LIN Mei,WEN Mingxia,FENG Xianju,ZHANG Weiqing,YAO Zhoulin,PING Xinliang.Status of available copper and zinc content in soil of orange orchards in Taizhou[J].Journal of Zhejiang Agricultural Sciences,2012,1(3):321-323.
[27]凌丽俐,彭良志,淳长品,曹立,江才伦.赣南纽荷尔脐橙叶片微量元素含量状况[J].园艺学报,2010,37(9):1388-1394.LING Lili,PENG Liangzhi,CHUN Changpin,CAO Li,JIANG Cailun.Characteristics analysis of mcroelement contents in Newhall navel orange leaves in southern Jiangxi province[J].Acta Horticulturae Sinica,2010,37(9):1388-1394.
[28]陈桂芬,黄玉溢,刘斌,江泽普.广西柑桔叶片铜、锌、铁、钙含量测定与营养分析[J].广西农业科学,2006,37(2):163-165.CHEN Guifen,HUANG Yuyi,LIU Bin,JIANG Zepu.Content determination of copper,iron,zinc and calcium and nutrient analysis in citrus leaves in Guangxi[J].Guangxi Agricultural Sciences,2006,37(2):163-165.
[29]黄玉溢,刘斌,陈桂芬,王影.广西柑桔园土壤、植株叶片及根的铜含量研究[J].西南农业学报,2007,20(5):1060-1063.HUANG Yuyi,LIU Bin,CHEN Guifen,WANG Ying.Content of copper in soil citrus leaves and branch roots of citrus orchards in Guangxi[J].Southwest China Journal of Agricultural Sciences,2007,20(5):1060-1063.
[30]周鑫斌,石孝均,温明霞,王秀英,孙彭寿,李伟,戴亨林,淳长品.三峡重庆库区甜橙叶片矿质营养丰缺状况调查[J].园艺学报,2011,38(10):1847-1856.ZHOU Xinbin,SHI Xiaojun,WEN Mingxia,WANG Xiuying,SUN Pengshou,LI Wei,DAI Henglin,CHUN Changpin.Orange leaves nutrient status of the Three Gorges Area in Chongqing[J].Acta Horticulturae Sinica,2011,38(10):1847-1856.
[31]杨惠栋,淳长品,彭良志,付行政,凌丽俐,江才伦,曹立,袁高鹏,张梦娇.奉节脐橙树体营养状况研究[J].中国南方果树,2016,45(2):10-15.YANG Huidong,CHUN Changpin,PENG Liangzhi,FU Xingzheng,LING Lili,JIANG Cailun,CAO Li,YUAN Gaopeng,ZHANG Mengjiao.Leaf nutrient status of navel orange in Fengjie,Chongqing[J].South China Fruits,2016,45(2):10-15.
[32]梁永元.柑桔缺铜症诊断法[J].贵州农业科学,1979(3):62-64.LIANG Yongyuan.Diagnositics of citrus copper deficiency[J].Guizhou Agricultural Sciences,1979(3):62-64.
[33]常红岩,孙百晔,刘春生.植物铜素毒害研究进展[J].山东农业大学学报(自然科学版),2000,31(2):227-230.CHANG Hongyan,SUN Baiye,LIU Chunsheng.Advances in the study of plants copper toxicity[J].Journal of Shandong Agricultural University(Natural Science),2000,31(2):227-230.
[34]王燕斌,平新亮,林媚.柑橘微量元素中毒症状及其防治[J].江西植保,2011,34(2):57-58.WANG Yanbin,PING Xinliang,LIN Mei.Toxic symptoms of citrus trace elements and control measures[J].Jiangxi Plant Protection,2011,34(2):57-58.
[35]黎耿碧,陈二钦,ALVA A K.外界铜离子浓度对柑桔小苗常量元素吸收特性的影响[J].广西农业大学学报,1996,15(3):15-21.LI Gengbi,CHEN Erqin,ALVA A K.Effects of external copper concentrations on uptake of routine elements by citrus seedling[J].Journal of Guangxi Agricultural University,1996,15(3):15-21.
[36]REUTHER W,SMITH P F.Effects of high copper content of sandy soil on growth on citrus seedlings[J].Soil Science,1953,5(3):219-224.
[37]ALVA A K,CHEN E Q.Effects of external copper concentrations on uptake of trace elements by citrus seedings[J].Soil Science,1995,159(1):59-64.
[38]YUAN H M,XU H H,LIU W C,LU Y T.Copper regulates primary root elongation through PIN1-mediated auxin redistribution[J].Plant Cell Physiology,2013,54(5):766-778.
[39]IDUNA A,DOUGLAS L G,ANTONINO O.Influence of copper on root growth and morphology of Pinus pinea L.and Pinus pinaster Ait.seedlings[J].Tree Physiology,1995,15(6):411-415.
[40]MESEJO C,MARTINEZ-FUENTES A,REIG C,RIVAS F,AGUSTI M.The inhibitory effect of Cu SO4on Citrus pollen germination and pollen tube growth and its application for the production of seedless fruit[J].Plant Science,2006,170(1):37-43.
[41]BEHLAU F,BELASQUE Jr J,GRAHAM J H,LEITE Jr R P.Effect of frequency of copper applications on control of citrus canker and the yield of young bearing sweet orange trees[J].Crop Protection,2010,29(3):300-305.
[42]ROBSON A D,HARTLEY R D,JARVIS S C.Effect of copper deficiency on phenolic and other constituents of wheat cell walls[J].New Phytologist,1981,89(3):361-371.
[43]SCHUTTE K H,MATHEWS M.An anatomical study of copper deficient wheat[J].Transactions Royal Society of South Arica,1969,38(2):183-200.
[44]FRY S C,DUMVILLE J C,MILLER J G.Fingerprinting of polysaccharides attacked by hydroxyl radicals in vitro and in the cell walls of ripening pear fruit[J].Biochemical Journal,2001,357(3):729-737.
[45]黄建国.植物营养学[M].北京:中国林业出版社,2003:107-231.HUANG Jianguo.Plant nutriology[M].Beijing:China Forestry Publishing House,2003:107-231.
[46]刘婷婷,彭程,王梦,段德超,施积炎.海州香薷根细胞壁对铜的吸附固定机制研究[J].环境科学学报,2014,34(2):514-523.LIU Tingting,PENG Cheng,WANG Meng,DUAN Dechao,SHI Jiyan.Mechanism of fixation and adsorption of copper on root cell wall of Elsholtzia splendens[J].Acta Scientiae Circumstantiae,2014,34(2):514-523.
[47]LOU L,SHEN Z,LI X.The copper tolerance mechanisms of Elsholtzia haichowensi,a plant from copper-enriched soils[J].Environmental and Experimental Botany,2004,51(2):111-120.
[48]王三根.植物抗性生理与分子生物学[M].北京:现代教育出版社,2009:16-20.WANG Sangen.Plant hardiness physiology and molecular biology[M].Beijing:Modern Education Press,2009:16-20.
[49]贾彦博,芮昶,季天委,黄凌云,陈郊,罗春林.土壤铜污染的微生物及酶学指标研究[J].广东微量元素科学,2009,16(3):44-47.JIA Yanbo,RUI Chang,JI Tianwei,HUANG Lingyun,CHEN Jiao,LUO Chunlin.Study on copper pollution index of soil microbe and enzymes[J].Guangdong Trace Elements Science,2009,16(3):44-47.
[50]OUZOUNIDOU G,CIAMPOROVA M,MOUSTAKAS M,KARATAGLIS S.Responses of maize(Zea mays L.)plants to copper stress-I.Growth,mineral content and ultrastructure of roots[J].Environmental and Experimental Botany,1995,35(2):167-176.
[51]DONCHEVA S,NIKOLOV B,OGNEVA V.Effect of copper excess on the morphology of the nucleus in maize root meristem cells[J].Physiologia Plantarum,1996,96(1):118-122.
[52]ZAMBROSI F C B,MESQUITA G L,TANAKA F A O,QUAGGIO J A,MATTOS D.Phosphorus availability and rootstock affect copper-induce damage to the root ultra-structure of Citrus[J].Environmental and Experimental Botany,2013,95(11):25-33.
[53]秦遂初,骆永明,黄昌勇,吴政,竺陆春.小麦缺铜不实与花粉淀粉积累的关系[J].作物学报,1994,20(4):453-456.QIN Suichu,LUO Yongming,HUANG Changyong,WU Zheng,ZHU Luchun.Study on the relationship between the sterility in wheat caused by copper deficiency and the starch:accumulation in the pollen grains[J].Acta Agronomica Sinica,1994,20(4):453-456.
[54]黄奔立,伍烨,张顺琦,许云东,陈学好.矿质营养缺乏对黄瓜生理生化特性的影响[J].生物技术通报,2006(S1):326-329.HUANG Benli,WU Ye,ZHANG Shunqi,XU Yundong,CHEN Xuehao.Effects of deficiency of mineral nutrients on the physiological and biochemical characteristics of cucumber plant[J].Biotechnology Bulletin,2006(S1):326-329.
[55]CAMBROLLE J,MANCILLA-LEYTON J M,MUNOZVALLES S,FIGUEROA-LUQUE E,LUQUE T,FIGUEROA M E.Effects of copper sulfate on growth and physiological responses of Limoniastrum monopetalum[J].Environmental Science and Pollution Research,2013,20(12):8839-8847.
[56]HSU B,LEE J.Toxic effects of copper on photosystemⅡof spinach chloroplasts[J].Plant Physiology,1988,87(1):116-119.
[57]韩配配,秦璐,李银水,廖祥生,徐子先,余常兵,胡小加,谢立华,廖红,廖星.不同营养元素缺乏对甘蓝型油菜苗期生长和根系形态的影响[J].中国油料作物学报,2016,38(1):88-97.HAN Peipei,QIN Lu,LI Yinshui,LIAO Xiangsheng,XU Zixian,YU Changbing,HU Xiaojia,XIE Lihua,LIAO Hong,LIAO Xing.Effects of different nutrient deficiencies on growth and root morphological changes of rapeseed seedlings(Brassica napus L.)[J].Chinese Journal of Oil Crop Sciences,2016,38(1):88-97.
[58]HIPPLER F R,CIPRIANO D O,BOARETTO R M,QUAGGIO J A,GAZIOLA S A,AZEVEDO R A,MATTOS-JR D.Citrus rootstocks regulate the nutritional status and antioxidant system of trees under copper stress[J].Environmental and Experimental Botany,2016,130:42-52.
[59]LLORENS N,AROLA L,BLADéC,MAS A.Effects of copper exposure upon nitrogen metabolism in tissue cultured Vitis vinifera[J].Plant Science,2000,160(1):159-163.
[60]ALVA A K,HUANG B,PRAKASH O,PARAMASIVAM S.Effects of copper rates and soil p H on growth and nutrient uptake by citrus seedlings[J].Journal of Plant Nutrition,1999,22(11):1687-1699.
[61]刘春生,史衍玺,马丽,叶优良,杨吉华,张福锁.过量铜对苹果树生长及代谢的影响[J].植物营养与肥料学报,2000,6(4):451-456.LIU Chunsheng,SHI Yanxi,MA Li,YE Youliang,YANG Jihua,ZHANG Fusuo.Effect of excessive copper on growth and metabolism of apple trees[J].Plant Nutrition and Fertilizer Science,2000,6(4):451-456.
[62]GRAHAM J H,TIMMER L W,FARDELMANN D.Toxicity of fungicidal copper in soil to citrus seedlings and vesicular arbuscular mycorrhizal fungi[J].Phytopathology,1986,76(1):66-70.
[63]COHUY C M,PILON M.Regulation of superoxide dismutase expression by copper availability[J].Physiologia Plantarum,2007,129(4):747-755.
[64]杜亚琳.拟南芥缺铜敏感突变体的鉴定分析[J].贵州农业科学,2016,4(8):80-93.DU Yalin.Identification and analysis of copper deficiency sensitive mutant in Arabidopsis[J].Guizhou Agricultural Sciences,2016,4(8):80-93.
[65]MERCHANT S,ALLEN M D,KROPAT J,MOSELEY J L,LONG J C,TOTTEY S,TERAUCHI A M.Between a rock and a hard place:trace element nutrition in Chlamydomonas[J].Biochimica et Biophysica Acta,2006,1763(7):578-594.
[66]陈立松,刘星辉.果树重金属毒害及抗性机理[J].福建农业大学学报,2001,30(4):462-469.CHEN Lisong,LIU Xinghui.Heavy metal toxicity and mechanisms of resistance in fruit trees[J].Journal of Fujian Agricultural University,2001,30(4):462-469.
[67]江行玉,赵可夫.植物重金属伤害及其抗性机理[J].应用与环境生物学报,2001,7(1):92-99.JIANG Xingyu,ZHAO Kefu.Mechanism of heavy metal injury and resistance of plants[J].Chinese Journal of Applied and Environmental Biology,2001,7(1):92-99.
[68]SALT D E,SMITH R D,RASKIN I.Phytoremediation[J].Annual Review of Plant Biology,1998,49(49):643-668.
[69]HE Z,ALVA A K,CALVERT D V,LI Y C,BANKS D J.Effects of nitrogen fertilization of grapefruit trees on soil acidification and nutrient availability in a Riviera fine sand[J].Plant and Soil,1999,206(1):11-19.
[70]ALVA A K,HUANG B,PARAMASIVAM S.Soil p H affects copper fractionation and phytotoxicity[J].Soil Science Society of America Journal,2000,64(3):955-962.
[71]CHAPMAN H D E.Diagnostic criteria for plants and soils[M].California:University of California,1966:157-179.
[72]BINGHAM F T,MARTIN J P,CHASTAIN J A.Effects of phosphorus fertilization of California soils on minor element nutrition of citrus[J].Soil Science,1958,86(1):24-31.
[73]ALVA A K,GRAHAM J H,TUCKER D H.Role of calcium in amelioration of copper phytotoxicity for Citrus[J].Soil Science,1993,155(3):211-218.
[74]CHUNDAWAT B S,KHERA A P,ARORA R K.Tissue micronutrients content of some stionic combinations in sweet orange(Citrus sinensis L.Osbeck)[J].Indian Journal of Horticulture,1983,40(3/4):45-48.
[75]JAWANDA J S,MEHROTRA N K.Pre-bearing performance of five sweet-orange cultivars on different root stocks[J].Indian Journal of Horticulture,1974,3:19-22.
[76]覃永祝.柑桔容器苗缺铜的治方法研究[J].中国南方果树,2005,34(2):18-19.TAN Yongzhu.Study on the treatment of copper deficiency in citrus container[J].South China Fruits,2005,34(2):18-19.
[77]COBBETT C,GOLDSBROUGH P.Phytochelatins and metallothioneins:roles in heavy metal detoxification and homeostasis[J].Annual Review of Plant Biology,2002,53(1):159.
[78]SEKHAR K,PRIYANKA B,REDDY V D,RAO K V.Metallothionein1(Cc MT1)of pigeonpea(Cajanus cajan L.)confers enhanced tolerance to copper and cadmium in Escherichia coli and Arabidopsis thaliana[J].Environmental and Experimental Botany,2011,72(2):131-139.
[79]叶俊丽,刘永忠,陶能国,曾文芳,邓秀新.一个新的甜橙金属硫蛋白基因Cs MT1的鉴定及功能初探[C]//杨凌:中国园艺学会学术年会,2012.YE Junli,LIU Yongzhong,TAO Nengguo,ZENG Wenfang,DENG Xiuxin.A study on the identification and function of a new metallothionein gene Cs MT1 in sweet orange[C]//Yangling:The Academic Annual Conference of Chinese Society for Horticultural Science,2012.
[80]BLUM R,BECK A,KORTE A,STENGEL A,LETZEL T,LENDZIAN K,GRILL E.Function of phytochelatin synthase in catabolism of glutathione-conjugates[J].Plant Journal,2007,49(4):740-749.
[81]VATAMANJUK O K,MARI S,LU Y P,REA P A.Mechanism of heavy metal ion activation of phytochelatin(PC)synthase:blocked thiols are sufficient for PC synthase-catalyzed transpeptidation of glutathione and related thiol peptides[J].Journal of Biological Chemistry,2000,275(40):31451-31459.
[82]XIANG C B,OLIVER D J.Glutathione metabolic genes coordinately respond to heavy metals and jasmonic acid in Arabidopsis[J].Plant Cell,1998,10(9):1539-1550.
[83]金枫,王翠,林海建,沈亚欧,张志明,赵茂俊,潘光堂.植物重金属转运蛋白研究进展[J].应用生态学报,2010,21(7):1875-1882.JIN Feng,WANG Cui,LIN Haijian,SHEN Yaou,ZHANG Zhiming,ZHAO Maojun,PAN Guangtang.Review of heavy metal transport proteins in plants[J].Chinese Journal of Applied Ecology,2010,21(7):1875-1882.
[84]UENO D,YAMA J N,MA J F.Further characterization of ferricphytosiderophore transporters Zm YS1 and Hv YS1 in maize and barley[J].Journal of Experimental Botany,2009,60(12):3513-3520.
[85]DIDONATO JR R J,ROBERTS L A,SANDERSON T,EISLEY R B,WALKER E L.Arabidopsis Yellow Stripe-Like2(YSL2):a metal-regulated gene encodinga plasma membrane transporter of nicotianamine-metal complexes[J].Plant Journal,2004,39(3):403-414.
[86]WINTZ H,FOX T,WU Y,FENG V,CHEN W Q,CHANG H S,ZHU T,VULPE C.Expression profiles of Arabidopsis thaliana in mineral deficiencies reveal novel transporters involved in metal homeostasis[J].Journal of Biological Chemistry,2003,278(48):47644-47653.
[87]SANCENONóN V,PUIG S,MIRA H,THIELE D J,PENARRUBIA L.Identification of a copper transporter family in Arabidopsis thaliana[J].Plant Molecular Biology,2003,51(4):577-587.
[88]YRUELA I.Copper in plants:Acquisition,transport and interactions[J].Functional Plant Biology,2009,36(5):409-430.
[89]ABDEL-GHANY S E,MüLLER-MOULéP,NIYOGI K,PILON M,SHIKANAI T.Two P-type ATPases are required for copper delivery in Arabidopsis thaliana chloroplasts[J].The Plant Cell,2005,17(4):1233-1251.
[90]FU X Z,TONG Y H,ZHOU X,LIN L L,CHUN C P,CAO L,ZENG M,PENG L Z.Genome-wide identification of sweet orange(Citrus sinensis)metal tolerance proteins and analysis of their expression patterns under zinc,manganese copper,and cadmium toxicity[J].Gene,2017,629:1-8.
[91]姚廷山,周彦,周常勇.应用铜制剂防治柑橘溃疡病的研究进展[J].园艺学报,2016,43(9):1711-1718.YAO Tingshan,ZHOU Yan,ZHOU Changyong.Advances in copper resistant mechanisms and control methods of citrus canker[J].Acta Horticulturae Sinica,2016,43(9):1711-1718.
[2]庄伊美.柑桔营养与施肥[M].北京:中国农业出版社,1994:42-43.ZHUANG Yimei.The nutrition and fertilization of Citrus[M].Beijing:China Agricultural Press,1994:42-43.
[3]唐玉琴,彭良志,淳长品,凌丽俐,方贻文,严翔.红壤甜橙园土壤和叶片营养元素相关性分析[J].园艺学报,2013,40(4):623-632.TANG Yuqin,PENG Liangzhi,CHUN Changpin,LING Lili,FANG Yiwen,YAN Xiang.Correlation analysis on nutrient element contents in orchard soils and sweet orange leaves in southern Jiangxi province of China[J].Acta Horticulturae Scinca,2013,40(4):623-632.
[4]陶梦铭,应虹,孙强,谭启玲,李翠,胡承孝.湖北与湖南柑橘园土壤及树体养分状况[J].湖北农业科学,2016,55(13):3289-3292.TAO Mengming,YING Hong,SUN Qiang,TAN Qiling,LI Cui,HU Chengxiao.Soil and plant nutrient status of citrus orchard in Hubei and Hunan province[J].Hubei Agricultural Sciences,2016,55(13):3289-3292.
[5]刘铮,朱其清,唐丽华,徐俊祥,尹楚良.我国缺乏微量元素的土壤及其区域分布[J].土壤学报,1982,19(3):209-223.LIU Zheng,ZHU Qiqing,TANG Lihua,XU Junxiang,YIN Chuliang.Geographical distribution of trace elements-deficient soils in China[J].Acta Pedologica Sinica,1982,19(3):209-223.
[6]曹立,彭良志,淳长品,凌丽俐,赖九江,闵嗣璠,江才伦.赣南不同土壤类型脐橙园叶片营养状况研究[J].中国南方果树,2012,41(2):5-9.CAO Li,PENG Liangzhi,CHUN Changpin,LING Lili,LAI Jiujiang,MIN Sifan,JIANG Cailun.Study on leaf nutrition of Newhall Orange on different soil types in southern Jiangxi[J].South China Fruits,2012,41(2):5-9.
[7]曾艳,黄金生,周柳强,谢如林,刘永贤,谭裕模,谭宏伟.广西桂南蔗区土壤养分状况调查分析[J].南方农业学报,2014,45(12):2198-2202.ZENG Yan,HUANG Jinsheng,ZHOU Liuqiang,XIE Rulin,LIU Yongxian,TAN Yumo,TAN Hongwei.Investigation of soil nutrient status in sugarcane-growing area of south Guangxi[J].Journal of Southern Agriculture,2014,45(12):2198-2202.
[8]杨夫臣,涂俊凡,秦仲麒,李先明,朱红艳,伍涛.湖北省砂梨主产区梨园土壤营养状况评价[J].农业科技通讯,2014(6):97-100.YANG Fucheng,TU Junfan,QIN Zhonglin,LI Xianming,ZHU Hongyan,WU Tao.Study on soil nutrient status in pear-growing area of Hubei[J].Bulletin of Agricultural Science and Technology,2014(6):97-100.
[9]俄胜哲,袁继超,丁志勇,姚凤娟,喻小平,罗付香.氮磷钾肥对稻米铁、锌、铜、锰、镁、钙含量和产量的影响[J].中国水稻科学,2005,19(5):434-440.E Shengzhe,YUAN Jichao,DING Zhiyong,YAO Fengjuan,YU Xiaoping,LUO Fuxiang.Effect of N,P,K fertilizers on Fe,Zn,Cu,Mn,Ca and Mg contents and yields in rice[J].Chinese Journal of Rice Science,2005,19(5):434-440.
[10]TIMMER L W,LEYDEN R F.The relationship of mycorrhizal infection to phosphorus-induced copper deficiency in sour orange seedlings[J].New Phytologist,1980,85(1):15-23.
[11]LIMA J S.Copper balances in cocoa agrarian ecosystems:effects of differential use of cupric fungicides[J].Agriculture,Ecosystems and Environment,1994,48(1):19-25.
[12]FAN J H,HE Z L,LENA Q M,STOFFELLA P J.Accumulation and availability of copper in citrus grove soils as affected by fungicide application[J].Journal of Soils and Sediments,2011,11(4):639-648.
[13]徐秋桐,张莉,章明奎.长期喷施波尔多液对葡萄园土壤、树体和径流中铜积累的影响[J].水土保持学报,2014,28(2):195-198.XU Qiutong,ZHANG Li,ZHANG Mingkui.Accumulation of copper in soil,plant tissue,amd runoff in vineyard as affected by long-term application of Bordeax mixture[J].Journal of Soil and Water Conservation,2014,28(2):195-198.
[14]潘琼,程运林.土壤重金属污染现状调查与评价[J].环境与可持续发展,2013,38(6):47-49.PAN Qiong,CHENG Yunlin.Soil heavy metal pollution present situation investigation and evaluation[J].Environment and Sustainable Development,2013,38(6):47-49.
[15]卜元卿,石利利,单正军.波尔多液在苹果和土壤中残留动态及环境风险评价[J].农业环境科学学报,2013,32(5):972-978.BU Yuanqing,SHI Lili,SHAN Zhengjun.Residue dynamic and environmental risk assessment of Bordeaux mixture in apple and soil[J].Journal of Agro-Environment Science,2013,32(5):972-978.
[16]张旭,曲燕,梁东丽,何洁,孙鑫.全国主要土壤铜、锌、镍重金属形态及其与土壤性质的关系[J].环境科学导刊,2016,35(5):9-14.ZHANG Xu,QU Yan,LIANG Dongli,HE Jie,SUN Xin.Primary study on the relationships between heavy metal speciation and their soil properties in China[J].Environmental Science Survey,2016,35(5):9-14.
[17]王秀丽,徐建民,姚槐应,谢正苗.重金属铜、锌、镉、铅复合污染对土壤环境微生物群落的影响[J].环境科学学报,2003,23(1):22-27.WANG Xiuli,XU Jianmin,YAO Huaiying,XIE Zhengmiao.Effects of Cu,Zn,Cd and Pb compound contamination on soil microbial community[J].Acta Scientiae Circumstantiae,2003,23(1):22-27.
[18]王男麒,彭良志,淳长品,李勋,薛珺,范玉兰,梁梅青.赣南柑桔园背景土壤营养状况分析[J].中国南方果树,2012,41(5):1-4.WANG Nanqi,PENG Liangzhi,CHUN Changpin,LI Xun,XUE Jun,FAN Yulan,LIANG Meiqing.Study on virgin soil nutrient status of citrus orchards in southern Jiangxi province[J].South China Fruits,2012,41(5):1-4.
[19]范西宁,黄玉溢,刘斌,陈桂芬.广西红壤柑桔园土壤剖面有效微量元素含量研究[J].广西农业科学,2007,38(3):285-287.FAN Xining,HUANG Yuyi,LIU Bin,CHEN Guifen.Contents of available microelements in soil profiles of citrus orchards with red soil in Guangxi[J].Guangxi Agricultural Sciences,2007,38(3):285-287.
[20]余红兵,王仁才,肖润林,杨知建.桂西北环境移民示范区柑橘园土壤和叶片营养状况[J].湖南农业大学学报(自然科学版),2007,33(3):341-347.YU Hongbing,WANG Rencai,XIAO Runlin,YANG Zhijian.Soil and leaf nutrient condition of plants from Citrus orchards in demonstration area of environmental immigrants,northwest Guangxi province[J].Journal of Hunan Agricultural University(Natural Sciences),2007,33(3):341-347.
[21]范玉兰,卢映琼,李峰,薛珺,李勋,钟八莲,彭良志.赣南脐橙园土壤有效铜含量分布特征研究[J].果树学报,2015,32(1):69-73.FAN Yulan,LU Yingqiong,LI Feng,XUE Jun,LI Xun,ZHONG Balian,PENG Liangzhi.Study on distribution of available copper content in soil of navel orange orchards in southern Jiangxi province[J].Journal of Fruit Science,2015,32(1):69-73.
[22]周鑫斌,石孝均,孙彭寿,李伟,戴亨林,彭良志,淳长品.三峡重庆库区柑橘园土壤养分丰缺状况研究[J].植物营养与肥料学报,2010,16(4):817-823.ZHOU Xinbin,SHI Xiaojun,SUN Pengshou,LI Wei,DAI Henglin,PENG Liangzhi,CHUN Changpin.Status of soil fertility in citrus orchards of Chongqing Sanxia Reservoir Area[J].Plant Nutrition and Fertilizer,2010,16(4):817-823.
[23]习建龙,彭良志,淳长品,朱春钊,杨惠栋,王男麒,江才伦,凌丽俐.重庆三峡库区加工甜橙园土壤养分状况分析[J].中国南方果树,2015,44(1):1-7.XI Jianlong,PENG Liangzhi,CHUN Changpin,ZHU Chunzhao,YANG Huidong,WANG Nanqi,JIANG Cailun,LING Lili.Analysis of nutriention status of the soil in processing orange orchards in Three Gorges Reservoir Region of Chongqing[J].South China Fruits,2015,44(1):1-7.
[24]鲁剑巍,陈防,王富华,刘冬碧,万运帆,余常兵,胡芳林,王明锐,王运华.湖北省柑橘园土壤养分分级研究[J].植物营养与肥料学报,2002,8(4):390-394.LU Jianwei,CHEN Fang,WANG Fuhua,LIU Dongbi,WAN Yunfan,YU Changbing,HU Fanglin,WANG Mingrui,WANG Yunhua.Study of classification of the soil nutrient status of citrus orchard in Hubei province[J].Plant Nutrition and Fertilizer Science,2002,8(4):390-394.
[25]应介官,刘秀红,李江波,吴群,彭抒昂,姜存仓.江西南丰和浙江衢州柑桔园土壤及叶片铜、锰营养状况调查[J].中国南方果树,2016,45(6):15-18.YING Jieguan,LIU Xiuhong,LI Jiangbo,WU Qun,PENG Shu’ang,JIANG Cuncang.Analysis of Cu and Mn contents in the soil and leaves of citrus orchards in Nanfeng and Quzhou[J].South China Fruits,2016,45(6):15-18.
[26]林媚,温明霞,冯先桔,张伟清,姚周麟,平新亮.台州柑橘园土壤有效铜锌养分现状[J].浙江农业科学,2012,1(3):321-323.LIN Mei,WEN Mingxia,FENG Xianju,ZHANG Weiqing,YAO Zhoulin,PING Xinliang.Status of available copper and zinc content in soil of orange orchards in Taizhou[J].Journal of Zhejiang Agricultural Sciences,2012,1(3):321-323.
[27]凌丽俐,彭良志,淳长品,曹立,江才伦.赣南纽荷尔脐橙叶片微量元素含量状况[J].园艺学报,2010,37(9):1388-1394.LING Lili,PENG Liangzhi,CHUN Changpin,CAO Li,JIANG Cailun.Characteristics analysis of mcroelement contents in Newhall navel orange leaves in southern Jiangxi province[J].Acta Horticulturae Sinica,2010,37(9):1388-1394.
[28]陈桂芬,黄玉溢,刘斌,江泽普.广西柑桔叶片铜、锌、铁、钙含量测定与营养分析[J].广西农业科学,2006,37(2):163-165.CHEN Guifen,HUANG Yuyi,LIU Bin,JIANG Zepu.Content determination of copper,iron,zinc and calcium and nutrient analysis in citrus leaves in Guangxi[J].Guangxi Agricultural Sciences,2006,37(2):163-165.
[29]黄玉溢,刘斌,陈桂芬,王影.广西柑桔园土壤、植株叶片及根的铜含量研究[J].西南农业学报,2007,20(5):1060-1063.HUANG Yuyi,LIU Bin,CHEN Guifen,WANG Ying.Content of copper in soil citrus leaves and branch roots of citrus orchards in Guangxi[J].Southwest China Journal of Agricultural Sciences,2007,20(5):1060-1063.
[30]周鑫斌,石孝均,温明霞,王秀英,孙彭寿,李伟,戴亨林,淳长品.三峡重庆库区甜橙叶片矿质营养丰缺状况调查[J].园艺学报,2011,38(10):1847-1856.ZHOU Xinbin,SHI Xiaojun,WEN Mingxia,WANG Xiuying,SUN Pengshou,LI Wei,DAI Henglin,CHUN Changpin.Orange leaves nutrient status of the Three Gorges Area in Chongqing[J].Acta Horticulturae Sinica,2011,38(10):1847-1856.
[31]杨惠栋,淳长品,彭良志,付行政,凌丽俐,江才伦,曹立,袁高鹏,张梦娇.奉节脐橙树体营养状况研究[J].中国南方果树,2016,45(2):10-15.YANG Huidong,CHUN Changpin,PENG Liangzhi,FU Xingzheng,LING Lili,JIANG Cailun,CAO Li,YUAN Gaopeng,ZHANG Mengjiao.Leaf nutrient status of navel orange in Fengjie,Chongqing[J].South China Fruits,2016,45(2):10-15.
[32]梁永元.柑桔缺铜症诊断法[J].贵州农业科学,1979(3):62-64.LIANG Yongyuan.Diagnositics of citrus copper deficiency[J].Guizhou Agricultural Sciences,1979(3):62-64.
[33]常红岩,孙百晔,刘春生.植物铜素毒害研究进展[J].山东农业大学学报(自然科学版),2000,31(2):227-230.CHANG Hongyan,SUN Baiye,LIU Chunsheng.Advances in the study of plants copper toxicity[J].Journal of Shandong Agricultural University(Natural Science),2000,31(2):227-230.
[34]王燕斌,平新亮,林媚.柑橘微量元素中毒症状及其防治[J].江西植保,2011,34(2):57-58.WANG Yanbin,PING Xinliang,LIN Mei.Toxic symptoms of citrus trace elements and control measures[J].Jiangxi Plant Protection,2011,34(2):57-58.
[35]黎耿碧,陈二钦,ALVA A K.外界铜离子浓度对柑桔小苗常量元素吸收特性的影响[J].广西农业大学学报,1996,15(3):15-21.LI Gengbi,CHEN Erqin,ALVA A K.Effects of external copper concentrations on uptake of routine elements by citrus seedling[J].Journal of Guangxi Agricultural University,1996,15(3):15-21.
[36]REUTHER W,SMITH P F.Effects of high copper content of sandy soil on growth on citrus seedlings[J].Soil Science,1953,5(3):219-224.
[37]ALVA A K,CHEN E Q.Effects of external copper concentrations on uptake of trace elements by citrus seedings[J].Soil Science,1995,159(1):59-64.
[38]YUAN H M,XU H H,LIU W C,LU Y T.Copper regulates primary root elongation through PIN1-mediated auxin redistribution[J].Plant Cell Physiology,2013,54(5):766-778.
[39]IDUNA A,DOUGLAS L G,ANTONINO O.Influence of copper on root growth and morphology of Pinus pinea L.and Pinus pinaster Ait.seedlings[J].Tree Physiology,1995,15(6):411-415.
[40]MESEJO C,MARTINEZ-FUENTES A,REIG C,RIVAS F,AGUSTI M.The inhibitory effect of Cu SO4on Citrus pollen germination and pollen tube growth and its application for the production of seedless fruit[J].Plant Science,2006,170(1):37-43.
[41]BEHLAU F,BELASQUE Jr J,GRAHAM J H,LEITE Jr R P.Effect of frequency of copper applications on control of citrus canker and the yield of young bearing sweet orange trees[J].Crop Protection,2010,29(3):300-305.
[42]ROBSON A D,HARTLEY R D,JARVIS S C.Effect of copper deficiency on phenolic and other constituents of wheat cell walls[J].New Phytologist,1981,89(3):361-371.
[43]SCHUTTE K H,MATHEWS M.An anatomical study of copper deficient wheat[J].Transactions Royal Society of South Arica,1969,38(2):183-200.
[44]FRY S C,DUMVILLE J C,MILLER J G.Fingerprinting of polysaccharides attacked by hydroxyl radicals in vitro and in the cell walls of ripening pear fruit[J].Biochemical Journal,2001,357(3):729-737.
[45]黄建国.植物营养学[M].北京:中国林业出版社,2003:107-231.HUANG Jianguo.Plant nutriology[M].Beijing:China Forestry Publishing House,2003:107-231.
[46]刘婷婷,彭程,王梦,段德超,施积炎.海州香薷根细胞壁对铜的吸附固定机制研究[J].环境科学学报,2014,34(2):514-523.LIU Tingting,PENG Cheng,WANG Meng,DUAN Dechao,SHI Jiyan.Mechanism of fixation and adsorption of copper on root cell wall of Elsholtzia splendens[J].Acta Scientiae Circumstantiae,2014,34(2):514-523.
[47]LOU L,SHEN Z,LI X.The copper tolerance mechanisms of Elsholtzia haichowensi,a plant from copper-enriched soils[J].Environmental and Experimental Botany,2004,51(2):111-120.
[48]王三根.植物抗性生理与分子生物学[M].北京:现代教育出版社,2009:16-20.WANG Sangen.Plant hardiness physiology and molecular biology[M].Beijing:Modern Education Press,2009:16-20.
[49]贾彦博,芮昶,季天委,黄凌云,陈郊,罗春林.土壤铜污染的微生物及酶学指标研究[J].广东微量元素科学,2009,16(3):44-47.JIA Yanbo,RUI Chang,JI Tianwei,HUANG Lingyun,CHEN Jiao,LUO Chunlin.Study on copper pollution index of soil microbe and enzymes[J].Guangdong Trace Elements Science,2009,16(3):44-47.
[50]OUZOUNIDOU G,CIAMPOROVA M,MOUSTAKAS M,KARATAGLIS S.Responses of maize(Zea mays L.)plants to copper stress-I.Growth,mineral content and ultrastructure of roots[J].Environmental and Experimental Botany,1995,35(2):167-176.
[51]DONCHEVA S,NIKOLOV B,OGNEVA V.Effect of copper excess on the morphology of the nucleus in maize root meristem cells[J].Physiologia Plantarum,1996,96(1):118-122.
[52]ZAMBROSI F C B,MESQUITA G L,TANAKA F A O,QUAGGIO J A,MATTOS D.Phosphorus availability and rootstock affect copper-induce damage to the root ultra-structure of Citrus[J].Environmental and Experimental Botany,2013,95(11):25-33.
[53]秦遂初,骆永明,黄昌勇,吴政,竺陆春.小麦缺铜不实与花粉淀粉积累的关系[J].作物学报,1994,20(4):453-456.QIN Suichu,LUO Yongming,HUANG Changyong,WU Zheng,ZHU Luchun.Study on the relationship between the sterility in wheat caused by copper deficiency and the starch:accumulation in the pollen grains[J].Acta Agronomica Sinica,1994,20(4):453-456.
[54]黄奔立,伍烨,张顺琦,许云东,陈学好.矿质营养缺乏对黄瓜生理生化特性的影响[J].生物技术通报,2006(S1):326-329.HUANG Benli,WU Ye,ZHANG Shunqi,XU Yundong,CHEN Xuehao.Effects of deficiency of mineral nutrients on the physiological and biochemical characteristics of cucumber plant[J].Biotechnology Bulletin,2006(S1):326-329.
[55]CAMBROLLE J,MANCILLA-LEYTON J M,MUNOZVALLES S,FIGUEROA-LUQUE E,LUQUE T,FIGUEROA M E.Effects of copper sulfate on growth and physiological responses of Limoniastrum monopetalum[J].Environmental Science and Pollution Research,2013,20(12):8839-8847.
[56]HSU B,LEE J.Toxic effects of copper on photosystemⅡof spinach chloroplasts[J].Plant Physiology,1988,87(1):116-119.
[57]韩配配,秦璐,李银水,廖祥生,徐子先,余常兵,胡小加,谢立华,廖红,廖星.不同营养元素缺乏对甘蓝型油菜苗期生长和根系形态的影响[J].中国油料作物学报,2016,38(1):88-97.HAN Peipei,QIN Lu,LI Yinshui,LIAO Xiangsheng,XU Zixian,YU Changbing,HU Xiaojia,XIE Lihua,LIAO Hong,LIAO Xing.Effects of different nutrient deficiencies on growth and root morphological changes of rapeseed seedlings(Brassica napus L.)[J].Chinese Journal of Oil Crop Sciences,2016,38(1):88-97.
[58]HIPPLER F R,CIPRIANO D O,BOARETTO R M,QUAGGIO J A,GAZIOLA S A,AZEVEDO R A,MATTOS-JR D.Citrus rootstocks regulate the nutritional status and antioxidant system of trees under copper stress[J].Environmental and Experimental Botany,2016,130:42-52.
[59]LLORENS N,AROLA L,BLADéC,MAS A.Effects of copper exposure upon nitrogen metabolism in tissue cultured Vitis vinifera[J].Plant Science,2000,160(1):159-163.
[60]ALVA A K,HUANG B,PRAKASH O,PARAMASIVAM S.Effects of copper rates and soil p H on growth and nutrient uptake by citrus seedlings[J].Journal of Plant Nutrition,1999,22(11):1687-1699.
[61]刘春生,史衍玺,马丽,叶优良,杨吉华,张福锁.过量铜对苹果树生长及代谢的影响[J].植物营养与肥料学报,2000,6(4):451-456.LIU Chunsheng,SHI Yanxi,MA Li,YE Youliang,YANG Jihua,ZHANG Fusuo.Effect of excessive copper on growth and metabolism of apple trees[J].Plant Nutrition and Fertilizer Science,2000,6(4):451-456.
[62]GRAHAM J H,TIMMER L W,FARDELMANN D.Toxicity of fungicidal copper in soil to citrus seedlings and vesicular arbuscular mycorrhizal fungi[J].Phytopathology,1986,76(1):66-70.
[63]COHUY C M,PILON M.Regulation of superoxide dismutase expression by copper availability[J].Physiologia Plantarum,2007,129(4):747-755.
[64]杜亚琳.拟南芥缺铜敏感突变体的鉴定分析[J].贵州农业科学,2016,4(8):80-93.DU Yalin.Identification and analysis of copper deficiency sensitive mutant in Arabidopsis[J].Guizhou Agricultural Sciences,2016,4(8):80-93.
[65]MERCHANT S,ALLEN M D,KROPAT J,MOSELEY J L,LONG J C,TOTTEY S,TERAUCHI A M.Between a rock and a hard place:trace element nutrition in Chlamydomonas[J].Biochimica et Biophysica Acta,2006,1763(7):578-594.
[66]陈立松,刘星辉.果树重金属毒害及抗性机理[J].福建农业大学学报,2001,30(4):462-469.CHEN Lisong,LIU Xinghui.Heavy metal toxicity and mechanisms of resistance in fruit trees[J].Journal of Fujian Agricultural University,2001,30(4):462-469.
[67]江行玉,赵可夫.植物重金属伤害及其抗性机理[J].应用与环境生物学报,2001,7(1):92-99.JIANG Xingyu,ZHAO Kefu.Mechanism of heavy metal injury and resistance of plants[J].Chinese Journal of Applied and Environmental Biology,2001,7(1):92-99.
[68]SALT D E,SMITH R D,RASKIN I.Phytoremediation[J].Annual Review of Plant Biology,1998,49(49):643-668.
[69]HE Z,ALVA A K,CALVERT D V,LI Y C,BANKS D J.Effects of nitrogen fertilization of grapefruit trees on soil acidification and nutrient availability in a Riviera fine sand[J].Plant and Soil,1999,206(1):11-19.
[70]ALVA A K,HUANG B,PARAMASIVAM S.Soil p H affects copper fractionation and phytotoxicity[J].Soil Science Society of America Journal,2000,64(3):955-962.
[71]CHAPMAN H D E.Diagnostic criteria for plants and soils[M].California:University of California,1966:157-179.
[72]BINGHAM F T,MARTIN J P,CHASTAIN J A.Effects of phosphorus fertilization of California soils on minor element nutrition of citrus[J].Soil Science,1958,86(1):24-31.
[73]ALVA A K,GRAHAM J H,TUCKER D H.Role of calcium in amelioration of copper phytotoxicity for Citrus[J].Soil Science,1993,155(3):211-218.
[74]CHUNDAWAT B S,KHERA A P,ARORA R K.Tissue micronutrients content of some stionic combinations in sweet orange(Citrus sinensis L.Osbeck)[J].Indian Journal of Horticulture,1983,40(3/4):45-48.
[75]JAWANDA J S,MEHROTRA N K.Pre-bearing performance of five sweet-orange cultivars on different root stocks[J].Indian Journal of Horticulture,1974,3:19-22.
[76]覃永祝.柑桔容器苗缺铜的治方法研究[J].中国南方果树,2005,34(2):18-19.TAN Yongzhu.Study on the treatment of copper deficiency in citrus container[J].South China Fruits,2005,34(2):18-19.
[77]COBBETT C,GOLDSBROUGH P.Phytochelatins and metallothioneins:roles in heavy metal detoxification and homeostasis[J].Annual Review of Plant Biology,2002,53(1):159.
[78]SEKHAR K,PRIYANKA B,REDDY V D,RAO K V.Metallothionein1(Cc MT1)of pigeonpea(Cajanus cajan L.)confers enhanced tolerance to copper and cadmium in Escherichia coli and Arabidopsis thaliana[J].Environmental and Experimental Botany,2011,72(2):131-139.
[79]叶俊丽,刘永忠,陶能国,曾文芳,邓秀新.一个新的甜橙金属硫蛋白基因Cs MT1的鉴定及功能初探[C]//杨凌:中国园艺学会学术年会,2012.YE Junli,LIU Yongzhong,TAO Nengguo,ZENG Wenfang,DENG Xiuxin.A study on the identification and function of a new metallothionein gene Cs MT1 in sweet orange[C]//Yangling:The Academic Annual Conference of Chinese Society for Horticultural Science,2012.
[80]BLUM R,BECK A,KORTE A,STENGEL A,LETZEL T,LENDZIAN K,GRILL E.Function of phytochelatin synthase in catabolism of glutathione-conjugates[J].Plant Journal,2007,49(4):740-749.
[81]VATAMANJUK O K,MARI S,LU Y P,REA P A.Mechanism of heavy metal ion activation of phytochelatin(PC)synthase:blocked thiols are sufficient for PC synthase-catalyzed transpeptidation of glutathione and related thiol peptides[J].Journal of Biological Chemistry,2000,275(40):31451-31459.
[82]XIANG C B,OLIVER D J.Glutathione metabolic genes coordinately respond to heavy metals and jasmonic acid in Arabidopsis[J].Plant Cell,1998,10(9):1539-1550.
[83]金枫,王翠,林海建,沈亚欧,张志明,赵茂俊,潘光堂.植物重金属转运蛋白研究进展[J].应用生态学报,2010,21(7):1875-1882.JIN Feng,WANG Cui,LIN Haijian,SHEN Yaou,ZHANG Zhiming,ZHAO Maojun,PAN Guangtang.Review of heavy metal transport proteins in plants[J].Chinese Journal of Applied Ecology,2010,21(7):1875-1882.
[84]UENO D,YAMA J N,MA J F.Further characterization of ferricphytosiderophore transporters Zm YS1 and Hv YS1 in maize and barley[J].Journal of Experimental Botany,2009,60(12):3513-3520.
[85]DIDONATO JR R J,ROBERTS L A,SANDERSON T,EISLEY R B,WALKER E L.Arabidopsis Yellow Stripe-Like2(YSL2):a metal-regulated gene encodinga plasma membrane transporter of nicotianamine-metal complexes[J].Plant Journal,2004,39(3):403-414.
[86]WINTZ H,FOX T,WU Y,FENG V,CHEN W Q,CHANG H S,ZHU T,VULPE C.Expression profiles of Arabidopsis thaliana in mineral deficiencies reveal novel transporters involved in metal homeostasis[J].Journal of Biological Chemistry,2003,278(48):47644-47653.
[87]SANCENONóN V,PUIG S,MIRA H,THIELE D J,PENARRUBIA L.Identification of a copper transporter family in Arabidopsis thaliana[J].Plant Molecular Biology,2003,51(4):577-587.
[88]YRUELA I.Copper in plants:Acquisition,transport and interactions[J].Functional Plant Biology,2009,36(5):409-430.
[89]ABDEL-GHANY S E,MüLLER-MOULéP,NIYOGI K,PILON M,SHIKANAI T.Two P-type ATPases are required for copper delivery in Arabidopsis thaliana chloroplasts[J].The Plant Cell,2005,17(4):1233-1251.
[90]FU X Z,TONG Y H,ZHOU X,LIN L L,CHUN C P,CAO L,ZENG M,PENG L Z.Genome-wide identification of sweet orange(Citrus sinensis)metal tolerance proteins and analysis of their expression patterns under zinc,manganese copper,and cadmium toxicity[J].Gene,2017,629:1-8.
[91]姚廷山,周彦,周常勇.应用铜制剂防治柑橘溃疡病的研究进展[J].园艺学报,2016,43(9):1711-1718.YAO Tingshan,ZHOU Yan,ZHOU Changyong.Advances in copper resistant mechanisms and control methods of citrus canker[J].Acta Horticulturae Sinica,2016,43(9):1711-1718.