红芪、黄芪及配伍当归对环磷酰胺所致血虚模型小鼠的干预作用
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摘要
目的:观察红芪、黄芪及配伍当归对环磷酰胺(CTX)所致血虚模型小鼠的干预作用。方法:昆明种小鼠随机分为7组,每组10只。采用CTX复制小鼠血虚模型,正常组与模型组小鼠用生理盐水灌胃,阳性对照组小鼠给予驴胶补血颗粒,四个给药组分别灌胃红芪、黄芪、红芪-当归组(5∶1)及黄芪-当归组(5∶1),连续灌胃7 d后,采用血细胞分析仪测定各组小鼠外周红细胞(RBC)、淋巴细胞(LYM)、红细胞压积(HCT)、白细胞(WBC)、血小板(PLT),取脾脏、胸腺、股骨,计算脾脏指数(SI)、胸腺指数(TI)的变化,进行网织红细胞计数(RC)、骨髓有核细胞计数,并对股骨进行病理切片观察。结果:与正常组比较,模型组小鼠RBC、WBC、HCT、PLT、LYM等含量均降低(P<0.05),与模型组比较,红芪及不同配伍各组小鼠中SI、TI均显著升高(P<0.05),红芪-当归组及黄芪-当归组可显著提高外周RBC、HCT、WBC、PLT、LYM的含量(P<0.05),可升高RC及骨髓有核细胞数量(P<0.05),股骨病理切片显示有所改善。结论:红芪-当归(5∶1)与黄芪-当归(5∶1)配伍对血虚模型小鼠的改善作用及补血作用优于红芪、黄芪的单独作用,黄芪-当归(5∶1)效果更佳。
Objective: To study the intervention effects of Radix Hedysari,Radix Astragalus and compatibility of Angelica Sinensis on blood deficiency model mice induced by cyclophosphamide( CTX). Methods: The mice were randomly divided into 7 groups,10 mice each group. The blood deficiency model was established by CTX. The blank group and model group were treated with saline by gavage,while mice in positive group were administered with Lvjiaobuxue granule. Four dosage group were administered with Radix Hedysari,Radix Hedysari-Radix Angelica Sinensis(5 ∶ 1),Radix Astragalus and Radix Astragalus-Radix Angelica Sinensis( 5 ∶ 1)water decoction. All the drugs were administered to mice for consecutive 7 d. The contents of red blood cell( RBC),lymphocyte(LYM),hematocrit(HCT),white blood cell(WBC),platelet(PLT) were detected by hematology analyzer,while thymus index(TI),spleen index(SI),reticulocyte(RC),marrow karyocyte(MK) were calculated,and the femur by pathological section were observed by microscope. Results: Compared with blank group,the contents of RBC,WBC,HCT,PLT,LYM were decreased in model group( P<0.05). Compared with model group,the contents of RBC,WBC,HCT,PLT,LYM,RC and marrow karyocyte were increased in Hedysari-Angelica Sinensis(5 ∶ 1) and Astragalus Angelica Sinensis(5 ∶ 1)( P<0.05),at the same time,the pathological damage of femur could be improved. Conclusion: The effect of enrichment blood on blood deficiency model mice in Hedysari-Angelica Sinensis(5 ∶ 1) and Astragalus-Angelica Sinensis(5 ∶ 1) were superior to Hedysari and Astragalus.
Objective: To study the intervention effects of Radix Hedysari,Radix Astragalus and compatibility of Angelica Sinensis on blood deficiency model mice induced by cyclophosphamide( CTX). Methods: The mice were randomly divided into 7 groups,10 mice each group. The blood deficiency model was established by CTX. The blank group and model group were treated with saline by gavage,while mice in positive group were administered with Lvjiaobuxue granule. Four dosage group were administered with Radix Hedysari,Radix Hedysari-Radix Angelica Sinensis(5 ∶ 1),Radix Astragalus and Radix Astragalus-Radix Angelica Sinensis( 5 ∶ 1)water decoction. All the drugs were administered to mice for consecutive 7 d. The contents of red blood cell( RBC),lymphocyte(LYM),hematocrit(HCT),white blood cell(WBC),platelet(PLT) were detected by hematology analyzer,while thymus index(TI),spleen index(SI),reticulocyte(RC),marrow karyocyte(MK) were calculated,and the femur by pathological section were observed by microscope. Results: Compared with blank group,the contents of RBC,WBC,HCT,PLT,LYM were decreased in model group( P<0.05). Compared with model group,the contents of RBC,WBC,HCT,PLT,LYM,RC and marrow karyocyte were increased in Hedysari-Angelica Sinensis(5 ∶ 1) and Astragalus Angelica Sinensis(5 ∶ 1)( P<0.05),at the same time,the pathological damage of femur could be improved. Conclusion: The effect of enrichment blood on blood deficiency model mice in Hedysari-Angelica Sinensis(5 ∶ 1) and Astragalus-Angelica Sinensis(5 ∶ 1) were superior to Hedysari and Astragalus.
引文
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[11]钟惠军,高英茂,董榕.环磷酰胺对大鼠胚胎DNA含量及神经上皮细胞有丝分裂的影响[J].中国药理学与毒理学杂志,1990,4(4):281.
[12]叶任高,余俊文,周霞,等.中药对环磷酰胺治疗狼疮性肾炎引起白细胞减少症的影响[J].中国中西医结合杂志,1994,14(1):43.
[13]刘永琦,王志旺,魏舒畅,等.超滤与醇沉对黄芪、红芪抗大鼠脑缺血的影响[J].中国应用生理学杂志,2015,31(2):132-135.
[14]王希梅,郭玫,王志旺,等.红芪总多糖及红芪多糖HG-1对大鼠骨关节炎的影响[J].中国应用生理学杂志,2018,34(3):273-276.
[2]刘卫民,韩艳欣,陈如泉.血虚证病因学的流行病学调查[J].中国中医基础医学杂志,1995(2):48-49.
[3]国家药典委员会.中华人民共和国药典[S].一部.北京:北京化学工业出版社,2015:284.
[4]李云志,黄静,郭弘川,等.红芪化学成分和抗肿瘤活性研究[J].中草药,2009,40(8):1195-1198.
[5]李俊岳,强正泽,李成义,等.红芪的本草考证[J].中国药房,2015,26(34):4860-4862.
[6]左之才,甘梦,崔恒敏,等.中药复方对骨髓抑制小鼠血液生理指标及细胞因子的影响[J].中国兽医科学,2013,43(5):537-544.
[7]陈光.实验室网织红细胞计数方法探讨[J].化工劳动保护:工业卫生与职业病分册,1995(6):309.
[8]袁金玲,伍勇,戴春梅.网织红细胞的三种计数方法的比较[J].中国现代医学杂志,2005,15(19):3001-3006.
[9]陆仁凤.网织红细胞计数方法的比较与评价[J].齐齐哈尔医学院报,2008,29(16):2004.
[10]朱映黎,王林元,王景霞,等.芍药内酯苷对环磷酰胺致血虚小鼠的补血作用及机制[J].中华中医药杂志,2016,31(5):1893-1896.
[11]钟惠军,高英茂,董榕.环磷酰胺对大鼠胚胎DNA含量及神经上皮细胞有丝分裂的影响[J].中国药理学与毒理学杂志,1990,4(4):281.
[12]叶任高,余俊文,周霞,等.中药对环磷酰胺治疗狼疮性肾炎引起白细胞减少症的影响[J].中国中西医结合杂志,1994,14(1):43.
[13]刘永琦,王志旺,魏舒畅,等.超滤与醇沉对黄芪、红芪抗大鼠脑缺血的影响[J].中国应用生理学杂志,2015,31(2):132-135.
[14]王希梅,郭玫,王志旺,等.红芪总多糖及红芪多糖HG-1对大鼠骨关节炎的影响[J].中国应用生理学杂志,2018,34(3):273-276.