SKP2、kindlin-2在前列腺癌组织中的表达及其与患者临床特征的关系
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摘要
目的探讨前列腺癌组织中细胞S相激酶相关蛋白2(SKP2)、kindlin-2蛋白的表达情况及其与患者临床特征的关系。方法选取70例前列腺癌患者的前列腺癌组织标本和同期70例前列腺良性增生患者的前列腺良性增生组织标本。采用免疫组织化学染色检测两种组织中SKP2、kindlin-2蛋白的阳性表达情况,并分析不同T分期、N分期、M分期、前列腺特异性抗原(PSA)水平、Gleason评分前列腺癌患者前列腺癌组织中SKP2、kindlin-2蛋白的阳性表达情况。结果前列腺癌患者前列腺癌组织中kindlin-2、SKP2蛋白的阳性表达率均明显高于前列腺良生增生患者的前列腺良性增生组织(P﹤0.01)。T分期为T3～T4期、N分期为N_1期、Gleason评分为8～10分前列腺癌患者前列腺癌组织中kindlin-2蛋白的阳性表达率均高于T分期为T_1～T_2期、N分期为N_0期、Gleason评分为2～7分的前列腺癌患者(P﹤0.05)。T分期为T_3～T_4期、N分期为N_1期、PSA水平﹥20 ng/ml、Gleason评分为8～10分前列腺癌患者前列腺癌组织中SKP2蛋白的阳性表达率均高于T分期为T_1～T_2期、N分期为N_0期、PSA水平≤20 ng/ml、Gleason评分为2～7分的前列腺癌患者(P﹤0.05)。结论前列腺癌组织中SKP2、kindlin-2蛋白的阳性表达率明显升高,并与前列腺癌的发生、发展密切相关。
Objective To investigate the expression of S-phase kinase associated protein 2(SKP2) and kindlin-2 in prostate cancer tissues and its relationship with clinical features. Method Prostate cancer tissue specimens from 70 patients with prostate cancer and another 70 specimens from patients with benign prostatic hyperplasia were collected. Immunohistochemical staining was used to detect the positive expression of SKP2 protein and kindlin-2 protein in these tissue specimens, additionally, the positive expression of SKP2 and kindlin-2 protein in prostate cancer tissues from patients with prostate cancer of different TNM stage, prostate specific antigen(PSA) level and Gleason scores were analyzed. RRee-sult The positive expression rates of kindlin-2 protein and SKP2 protein in prostate cancer tissues were significantly higher than those in benign prostatic hyperplasia tissues(P<0.01). The positive expression rate of kindlin-2 protein in prostate cancer tissues of patients with T_3-T_4, N_1, and Gleason score 8-10 diseases was higher than that in those with T_1-T_2, N_0 and Gleason scores of 2-7 diseases(P<0.05). In prostate cancer of T_3-T_4, N_1, PSA>20 ng/ml, and Gleason score 8-10, the positive expression rate of SKP2 protein was higher than that in patients with prostate cancer of T_1-T_2, N_0,PSA≤20 ng/ml, and Gleason score of 2-7 points(P<0.05). Conclusion The positive expression rate of SKP2 and kindlin-2 protein in prostate cancer tissues are significantly increased, which are closely related to the occurrence and development of prostate cancer.
Objective To investigate the expression of S-phase kinase associated protein 2(SKP2) and kindlin-2 in prostate cancer tissues and its relationship with clinical features. Method Prostate cancer tissue specimens from 70 patients with prostate cancer and another 70 specimens from patients with benign prostatic hyperplasia were collected. Immunohistochemical staining was used to detect the positive expression of SKP2 protein and kindlin-2 protein in these tissue specimens, additionally, the positive expression of SKP2 and kindlin-2 protein in prostate cancer tissues from patients with prostate cancer of different TNM stage, prostate specific antigen(PSA) level and Gleason scores were analyzed. RRee-sult The positive expression rates of kindlin-2 protein and SKP2 protein in prostate cancer tissues were significantly higher than those in benign prostatic hyperplasia tissues(P<0.01). The positive expression rate of kindlin-2 protein in prostate cancer tissues of patients with T_3-T_4, N_1, and Gleason score 8-10 diseases was higher than that in those with T_1-T_2, N_0 and Gleason scores of 2-7 diseases(P<0.05). In prostate cancer of T_3-T_4, N_1, PSA>20 ng/ml, and Gleason score 8-10, the positive expression rate of SKP2 protein was higher than that in patients with prostate cancer of T_1-T_2, N_0,PSA≤20 ng/ml, and Gleason score of 2-7 points(P<0.05). Conclusion The positive expression rate of SKP2 and kindlin-2 protein in prostate cancer tissues are significantly increased, which are closely related to the occurrence and development of prostate cancer.
引文
[1]王晓岩,许新征,尉迟今新,等. TRUS引导下前列腺穿刺活检联合PSA水平变化在诊断前列腺癌中的价值[J].癌症进展, 2017, 15(8):894-896.
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[4]任小强,张鹏,张建国. Kindlin-2在前列腺癌中的表达及其与临床病理特征和预后的关系[J].现代泌尿外科杂志,2018, 23(5):347-351.
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[8] Kim SH, Joung JY, Suh YS, et al. Prevalence and survival prognosis of prostate cancer in patients with end-stage renal disease:a retrospective study based on the Korea national database(2003-2010)[J]. Oncotarget, 2017, 8(38):64250-64262.
[9] Voog JC, Paulus R, Efstathiou JA. Decisions regarding whether to use androgen deprivation therapy with radiotherapy in prostate cancer:is cardiovascular mortality the most relevant outcome?[J]. Eur Urol, 2016, 69(2):211-212.
[10]吴杰,赵睿哲,黄源,等. PSA“灰区”前列腺癌预测模型及随访系统的建立[J].中华泌尿外科杂志, 2016, 37(4):280-283.
[11]李高翔,杨云杰,戴波.循环肿瘤标志物在前列腺癌研究中的新进展[J].中国癌症杂志, 2017, 27(10):833-838.
[12]马靖靖,刘付宝. PTEN、Kindlin-2和DcR3在老年结直肠癌的表达及其对预后的判断价值[J].中国老年学杂志,2015, 35(18):5175-5176.
[13]张芳,唐密,王建辉. Skp2和PTEN在上皮性卵巢肿瘤组织中的表达及临床意义[J].中国妇幼健康研究, 2016, 26(10):1217-1220.
[14]徐以民,刘海珍.有应用前景的前列腺癌相关肿瘤标志物[J].中华男科学杂志, 2015, 21(10):937-940.
[15]石玮,董莉,包军胜.前列腺癌相关分子研究进展[J].中华男科学杂志, 2015, 21(4):357-362.
[16]廖顺鸿.前列腺癌组织织中Skp2、p27的表达及临床意义[J].标记免疫分析与临床, 2018, 25(4):478-481.
[2] Monn MF, Tatem AJ, Cheng L. Prevalence and management of prostate cancer among East Asian men:Current trends and future perspectives[J]. Urol Oncol, 2016, 34(2):58. e1-e9.
[3]宋益挺,吴开杰,王新阳,等. S期激酶相关蛋白Skp2调控雄激素受体表达及活性在去势抵抗型前列腺癌进展中的作用[J].中华男科学杂志, 2016, 22(2):122-127.
[4]任小强,张鹏,张建国. Kindlin-2在前列腺癌中的表达及其与临床病理特征和预后的关系[J].现代泌尿外科杂志,2018, 23(5):347-351.
[5]中华医学会泌尿外科分会.前列腺癌诊断治疗指南[J].继续医学教育, 2007, 21(6):30-39.
[6]周萍,付勇,高文华,等. CD147和EGFR蛋白表达与胃癌转移和预后的关系[J].国际肿瘤学杂志, 2016, 43(3):184-187.
[7]胡忠贵.经腹腔与经腹膜外腹腔镜前列腺癌根治术疗效比较[J].现代仪器与医疗, 2016, 22(4):92-93.
[8] Kim SH, Joung JY, Suh YS, et al. Prevalence and survival prognosis of prostate cancer in patients with end-stage renal disease:a retrospective study based on the Korea national database(2003-2010)[J]. Oncotarget, 2017, 8(38):64250-64262.
[9] Voog JC, Paulus R, Efstathiou JA. Decisions regarding whether to use androgen deprivation therapy with radiotherapy in prostate cancer:is cardiovascular mortality the most relevant outcome?[J]. Eur Urol, 2016, 69(2):211-212.
[10]吴杰,赵睿哲,黄源,等. PSA“灰区”前列腺癌预测模型及随访系统的建立[J].中华泌尿外科杂志, 2016, 37(4):280-283.
[11]李高翔,杨云杰,戴波.循环肿瘤标志物在前列腺癌研究中的新进展[J].中国癌症杂志, 2017, 27(10):833-838.
[12]马靖靖,刘付宝. PTEN、Kindlin-2和DcR3在老年结直肠癌的表达及其对预后的判断价值[J].中国老年学杂志,2015, 35(18):5175-5176.
[13]张芳,唐密,王建辉. Skp2和PTEN在上皮性卵巢肿瘤组织中的表达及临床意义[J].中国妇幼健康研究, 2016, 26(10):1217-1220.
[14]徐以民,刘海珍.有应用前景的前列腺癌相关肿瘤标志物[J].中华男科学杂志, 2015, 21(10):937-940.
[15]石玮,董莉,包军胜.前列腺癌相关分子研究进展[J].中华男科学杂志, 2015, 21(4):357-362.
[16]廖顺鸿.前列腺癌组织织中Skp2、p27的表达及临床意义[J].标记免疫分析与临床, 2018, 25(4):478-481.