Adropin蛋白在新诊断2型糖尿病患者血清中的水平变化及其与胰岛素抵抗的相关性研究
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摘要
目的:探讨Adropin蛋白在新诊断2型糖尿病患者血清中的水平变化及其与胰岛素抵抗的关系。方法:采集佳木斯大学附属第一医院门诊及病房新诊断的2型糖尿病患者60例,按体质指数(BMI)分为两组,肥胖组(BMI≥25)与非肥胖组(BMI<25)各30例,同时还采集同一时间段的健康体检者30例为正常对照组。对其相关临床资料如性别、年龄、身高、体重、腰围(WC)、臀围(HC)等收集,并运用公式计算体质指数(BMI)及腰臀比(WHR),同时对生化指标如低密度脂蛋白(LDL-C)、甘油三酯(TG)、胆固醇(TC)、空腹血糖(FPG)、高密度脂蛋白(HDL-C)、糖化血红蛋白(HbA1c)、空腹胰岛素(FIns)等进行测定。运用公式计算胰岛素抵抗指数(HOMA-IR)及胰岛β细胞功能指数(HOMA-β),采用ELisa方法测定Adropin的水平。结果:(1)三组间性别、年龄、身高比较,差异无统计学意义。与对照组比较,2型糖尿病组中肥胖组与非肥胖组Adropin水平降低。(2)相关分析表明,血清Adropin水平与FPG、WC、HC、HbA1c、体重、BMI、TG、TC、HOMA-IR呈负相关,与HOMA-β、HDL呈正相关。(3)多元线性回归分析:HOMA-IR、TC、HC是Adropin的独立影响因素。结论:Adropin蛋白在新诊断2型糖尿病患者血清中水平是降低的,与HOMA-IR呈负相关,低Adropin水平可能参与了2型糖尿病的发生机制。
Objective:To investigate the changes of Adropin protein in serum and its relationship with insulin resistance in newly diagnosed patients with type 2 diabetes mellitus.Methods:60 cases of type 2 diabetes were collected from the outpatient and ward of the First Affiliated Hospital of Jiamusi University. According to the body mass index(BMI),two groups were divided into two groups,the obese group(BMI ≥25) and the non obese group(BMI<25) were 30 cases, and 30 normal controls were collected at the same time period. The related clinical data such as gender,age,height,weight,waist circumference(WC),hip circumference(HC),such as collection, and through the formula to calculate body mass index(BMI) and waist-to-hip ratio(WHR),at the same time and the biochemical indicators such as fasting plasma glucose(FPG), triglycerides(TG),cholesterol(TC),low density lipoprotein(LDL-C),high density lipoprotein(HDL-C),glycosylated hemoglobin(HbA1 c),fasting insulin(FIns) were measured.The formula was used to calculate insulin resistance index(HOMA-IR) and islet β cell function index(HOMA-β).The level of Adropin was determined by ELisa.Results:(1)There was no significant difference between the three groups in gender,age and height.Compared with the control group, the Adropin level of type 2 diabetes group was decreased.(2)Correlation analysis shows that serum Adropin level with FPG,WC,HC,HbA1 c,weight,BMI,TG,TC,HOMA-IR negative correlation,positively correlated with HOMA-β,HDL.(3)Multiple linear regression analysis:HOMA-IR,TC,HC is Adropin independent factors.Conclusion:The serum level of Adropin protein was decreased in newly diagnosed type 2 diabetes patients,which was negatively correlated with insulin resistance index.Low Adropin level may be involved in the occurrence mechanism of type 2 diabetes.
Objective:To investigate the changes of Adropin protein in serum and its relationship with insulin resistance in newly diagnosed patients with type 2 diabetes mellitus.Methods:60 cases of type 2 diabetes were collected from the outpatient and ward of the First Affiliated Hospital of Jiamusi University. According to the body mass index(BMI),two groups were divided into two groups,the obese group(BMI ≥25) and the non obese group(BMI<25) were 30 cases, and 30 normal controls were collected at the same time period. The related clinical data such as gender,age,height,weight,waist circumference(WC),hip circumference(HC),such as collection, and through the formula to calculate body mass index(BMI) and waist-to-hip ratio(WHR),at the same time and the biochemical indicators such as fasting plasma glucose(FPG), triglycerides(TG),cholesterol(TC),low density lipoprotein(LDL-C),high density lipoprotein(HDL-C),glycosylated hemoglobin(HbA1 c),fasting insulin(FIns) were measured.The formula was used to calculate insulin resistance index(HOMA-IR) and islet β cell function index(HOMA-β).The level of Adropin was determined by ELisa.Results:(1)There was no significant difference between the three groups in gender,age and height.Compared with the control group, the Adropin level of type 2 diabetes group was decreased.(2)Correlation analysis shows that serum Adropin level with FPG,WC,HC,HbA1 c,weight,BMI,TG,TC,HOMA-IR negative correlation,positively correlated with HOMA-β,HDL.(3)Multiple linear regression analysis:HOMA-IR,TC,HC is Adropin independent factors.Conclusion:The serum level of Adropin protein was decreased in newly diagnosed type 2 diabetes patients,which was negatively correlated with insulin resistance index.Low Adropin level may be involved in the occurrence mechanism of type 2 diabetes.
引文
[1] De Fronzo RA.From the triumvirate to the ominous octet: a new paradigm for the treatment of type 2 diabetes mellitus[J].Diabetes,2009,58(4):773-795.
[2] Kumar KG,Trevaskis JL,Lam DD,et al.Identification of Adropin as a secreted factorlinking dietary macronutrient intake with energy homeostasis and lipidmetabolism[J].Cell Metab,2008,8(6):468-481.
[3] Kersten S,Mandard S,Tan NS,et al.Characterization of the fastinginduced adipose factor FIAF,a novel peroxisome proliferator-activated receptor target gene[J].J Biol Chem,2000,275(37):28488-28493.
[4] 张亚南,李安荣,陈旭东,等.Adropin 蛋白的研究进展及临床意义[J]. 医学综述,2016,22(1):20-23.
[5] Samuel VT,Shulman GI.The pathogenesis of insulin resistance:integrating signaling pathways and sub-strate flux[J].J Clin Invest,2016,126(1):12-22.
[6] Wu L,Fang J,Chen L,et al.Low serum Adropin is associated with coronary atherosclerosis in type 2 diabetic and non-diabetic patients[J].Clinical Chemistry and Laboratory Medicine,2014,52(5):751-758.
[7] Butler AA,Tam CS,Stanhope KL,et al.Low circulating Adropin concentrations with obesity and aging correlate with risk factors for metabolic disease and increase after gastric bypass surgery in humans[J].J Clin Endocrinol Metab,2012,97(10):3783-3791.
[8] Ganesh Kumar K,Zhang J,Gao S,et al.Adropin deficiency is associated with increased adiposity and insulin resistance[J].Obesity (Silver Spring),2012,20(7):1394-1402.
[9] Gao S,Mcmillan RP,Zhu Q,et al.Therapeutic effects of Adropin on glucose tolerance and substrate utilization in diet-induced obese mice with insulin resistance[J].Mol Metab,2015,4(4):310.
[2] Kumar KG,Trevaskis JL,Lam DD,et al.Identification of Adropin as a secreted factorlinking dietary macronutrient intake with energy homeostasis and lipidmetabolism[J].Cell Metab,2008,8(6):468-481.
[3] Kersten S,Mandard S,Tan NS,et al.Characterization of the fastinginduced adipose factor FIAF,a novel peroxisome proliferator-activated receptor target gene[J].J Biol Chem,2000,275(37):28488-28493.
[4] 张亚南,李安荣,陈旭东,等.Adropin 蛋白的研究进展及临床意义[J]. 医学综述,2016,22(1):20-23.
[5] Samuel VT,Shulman GI.The pathogenesis of insulin resistance:integrating signaling pathways and sub-strate flux[J].J Clin Invest,2016,126(1):12-22.
[6] Wu L,Fang J,Chen L,et al.Low serum Adropin is associated with coronary atherosclerosis in type 2 diabetic and non-diabetic patients[J].Clinical Chemistry and Laboratory Medicine,2014,52(5):751-758.
[7] Butler AA,Tam CS,Stanhope KL,et al.Low circulating Adropin concentrations with obesity and aging correlate with risk factors for metabolic disease and increase after gastric bypass surgery in humans[J].J Clin Endocrinol Metab,2012,97(10):3783-3791.
[8] Ganesh Kumar K,Zhang J,Gao S,et al.Adropin deficiency is associated with increased adiposity and insulin resistance[J].Obesity (Silver Spring),2012,20(7):1394-1402.
[9] Gao S,Mcmillan RP,Zhu Q,et al.Therapeutic effects of Adropin on glucose tolerance and substrate utilization in diet-induced obese mice with insulin resistance[J].Mol Metab,2015,4(4):310.