摘要
使用超高效液相色谱-飞行时间质谱连续窗口采集所有理论碎片离子技术为基础的蛋白组学,对海捕和养殖中国对虾的蛋白进行相对定量,并通过化学计量学寻找区分海捕和养殖中国对虾的蛋白生物标志物。结果表明,筛选所得的蛋白生物标志物可以很好地区分海捕和养殖的中国对虾。以期为海捕和养殖中国对虾的区分提供一定的技术支持。
In this study, sequential window acquisition of all theoretical fragment ions(SWATH)-based proteomics was established for relative quantitation of proteins by ultra-high performance liquid chromatography coupled to quadrupole time of flight(UPLC-Q TOF) mass spectrometry. Chemometric tools were used to discover protein biomarkers for discrimination of wild and farmed Fenneropenaeus chinensis. The result showed that wild and farmed F. chinensis could be well discriminated through the protein biomarkers. This study may provide technical support for discrimination of wild and farmed F. chinensis.
引文
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