利用蛋白组学和化学计量学区分海捕和养殖的中国对虾
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摘要
使用超高效液相色谱-飞行时间质谱连续窗口采集所有理论碎片离子技术为基础的蛋白组学,对海捕和养殖中国对虾的蛋白进行相对定量,并通过化学计量学寻找区分海捕和养殖中国对虾的蛋白生物标志物。结果表明,筛选所得的蛋白生物标志物可以很好地区分海捕和养殖的中国对虾。以期为海捕和养殖中国对虾的区分提供一定的技术支持。
In this study, sequential window acquisition of all theoretical fragment ions(SWATH)-based proteomics was established for relative quantitation of proteins by ultra-high performance liquid chromatography coupled to quadrupole time of flight(UPLC-Q TOF) mass spectrometry. Chemometric tools were used to discover protein biomarkers for discrimination of wild and farmed Fenneropenaeus chinensis. The result showed that wild and farmed F. chinensis could be well discriminated through the protein biomarkers. This study may provide technical support for discrimination of wild and farmed F. chinensis.
In this study, sequential window acquisition of all theoretical fragment ions(SWATH)-based proteomics was established for relative quantitation of proteins by ultra-high performance liquid chromatography coupled to quadrupole time of flight(UPLC-Q TOF) mass spectrometry. Chemometric tools were used to discover protein biomarkers for discrimination of wild and farmed Fenneropenaeus chinensis. The result showed that wild and farmed F. chinensis could be well discriminated through the protein biomarkers. This study may provide technical support for discrimination of wild and farmed F. chinensis.
引文
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[23]张玥.吉林省大米产地溯源分析的研究[D].长春:吉林农业大学,2015.
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[25]MI R,SUN Y,LI J,et al.Immune-related proteins detected through iTRAQ-based proteomics analysis of intestines from Apostichopus japonicus in response to tussah immunoreactive substances[J].Fish&Shellfish Immunology,2018,74:436-443.DOI:10.1016/j.fsi.2018.01.002.
[26]VOWINCKEL J,CAPUANO F,CAMPBELL K,et al.The beauty of being(label)-free:sample preparation methods for SWATH-MS and next-generation targeted proteomics[J].F1000 Research,2013,2:272.DOI:10.12688/f1000research.2-272.v1.
[27]赵小梅,刘歆颖,续畅,等.基于LC-MS代谢组学的雷公藤多苷致肝毒性生物标志物的初步筛查[J].中国中药杂志,2015,40(19):3851-3858.
[28]牟菲.丹参-降香对大鼠心肌缺血/再灌注损伤的保护作用及机制的代谢组学研究[D].西安:第四军医大学,2017.
[29]YU Q,WU W,TIAN X,et al.Unraveling proteome changes of Holstein beef M.semitendinosus and its relationship to meat discoloration during post-mortem storage analyzed by label-free mass spectrometry[J].Journal of Proteomics,2017,154:85-93.DOI:10.1016/j.jprot.2016.12.012.
[30]WIKLUND S,JOHANSSON E,SJ諷TR諱L,et al.Visualization of GC/TOF-MS-based metabolomics data for identification of biochemically interesting compounds using OPLS class models[J].Analytical Chemistry,2008,80(1):115-122.DOI:10.1021/ac0713510.
[2]王陌桑.中国对虾增殖放流群体溯源分析及迁徙动态研究[D].上海:上海海洋大学,2016.
[3]陈建林.利用中国对虾加工副产物生产重组虾肉的研究[D].保定:河北农业大学,2014.
[4]侯晓荣.中国对虾的解冻方式研究和调理制品研制[D].杭州:浙江大学,2014.
[5]李姣,李学鹏,励建荣,等.冷藏条件下中国对虾肌肉蛋白质的生化特性[J].食品科学,2011,32(5):16-21.
[6]CHA H K,OH C W,HONG S Y,et al.Reproduction and population dynamics of Penaeus chinensis(Decapoda:Penaeidae)on the western coast of Korea,Yellow Sea[J].Fisheries Research,2002,56(1):25-36.DOI:10.1016/S0165-7836(01)00310-1.
[7]陈丽花,肖作兵,周培根.中国对虾蛋白质的营养价值评价[J].上海海洋大学学报,2009,18(1):88-93.
[8]楼伟风,李爱杰,徐家敏.中国对虾(Penaeus orientalis Kishiouys)粗蛋白、氨基酸含量的比较分析[J].中国海洋大学学报(自然科学版),1989(S2):69-79.
[9]PASCOAL A,BARROS-VELáZQUEZ J,ORTEA I,et al.Molecular identification of the black tiger shrimp(Penaeus monodon),the white leg shrimp(Litopenaeus vannamei)and the Indian white shrimp(Fenneropenaeus indicus)by PCR targeted to the 16S rRNAmtDNA[J].Food Chemistry,2011,125(4):1457-1461.DOI:10.1016/j.foodchem.2010.10.053.
[10]WANNA W,CHOTIGEAT W,PHONGDARA A.Sequence variations of the first ribosomal internal transcribed spacer of Penaeus species in Thailand[J].Journal of Experimental Marine Biology&Ecology,2006,331(1):64-73.DOI:10.1016/j.jembe.2005.10.008.
[11]崔维兴.人工养殖与海捕对虾(Penaeus orientalis Kishinouye)鉴别特征初探[J].河北渔业,1986(3):12-19
[12]林洪,姜凤英,JAMIL K,等.养殖和海捕对虾加热后蛋白特性变化的比较研究[J].水产学报,1998,22(2):143-147.
[13]薛长湖,曹杨,陈修白,等.养殖对虾与海捕对虾鉴别方法初探[J].中国海洋大学学报(自然科学版),1993(1):101-106.
[14]ORTEA I,GALLARDO J M.Investigation of production method,geographical origin and species authentication in commercially relevant shrimps using stable isotope rat io and/or mult ielement analyses combined with chemometrics:an exploratory analysis[J].Food Chemistry,2015,170(1):145-153.DOI:10.1016/j.foodchem.2014.08.049.
[15]KIM H,KUMAR K S,HWANG S Y,et al.Utility of stable isotope and cytochrome oxidase I gene sequencing analyses in inferring origin and authentication of hairtail fish and shrimp[J].Journal of Agricultural&Food Chemistry,2015,63(22):5548-5556.DOI:10.1021/acs.jafc.5b01469.
[16]ORTEA I,O’CONNOR G,MAQUET A.Review on proteomics for food authentication[J].Journal of Proteomics,2016,147(6):212-225.DOI:10.1016/j.jprot.2016.06.033.
[17]NICOLA G,DI GIUSEPPE A M A,MARTINA L,et al.Myoglobin as marker in meat adulteration:a UPLC method for determining the presence of pork meat in raw beef burger[J].Food Chemistry,2013,141(3):1814-1820.
[18]PIOVESANA S,CAPRIOTTI A L,CARUSO G,et al.Labeling and label free shotgun proteomics approaches to characterize muscle tissue from farmed and wild gilthead sea bream(Sparus aurata)[J].Journal of Chromatography A,2016,1428:193-201.DOI:10.1016/j.chroma.2015.07.049.
[19]BUCKLEY M,FRASER S,HERMAN J,et al.Species identification of archaeological marine mammals using collagen fingerprinting[J].Journal of Archaeological Science,2014,41(2):631-641.DOI:10.1016/j.jas.2013.08.021.
[20]SIMBRGER J M B,DETTMER K,OEFNER P J,et al.Optimizing the SWATH-MS-workflow for label-free proteomics[J].Journal of Proteomics,2016,145:137-140.DOI:10.1016/j.jprot.2016.04.021.
[21]GILLET L C,NAVARRO P,TATE S,et al.Targeted data extraction of the MS/MS spectra generated by data-independent acquisition:a new concept for consistent and accurate proteome analysis[J].Molecular&Cellular Proteomics,2012,11(6):1-7.DOI:10.1074/mcp.O111.016717.
[22]HU L P,ZHANG H W,ZHANG X M,et al.Identification of peptide biomarkers for discrimination of shrimp species through SWATHMS-based proteomics and chemometrics[J].Journal of Agricultural and Food Chemistry,2018,66(40):10567-10574.DOI:10.1021/acs.jafc.8b04375.
[23]张玥.吉林省大米产地溯源分析的研究[D].长春:吉林农业大学,2015.
[24]WISNIEWSKI J R,ZOUGMAN A,NAGARAJ N,et al.Universal sample preparation method for proteome analysis[J].Nature Methods,2009,6(5):359-362.DOI:10.1038/nmeth.1322.
[25]MI R,SUN Y,LI J,et al.Immune-related proteins detected through iTRAQ-based proteomics analysis of intestines from Apostichopus japonicus in response to tussah immunoreactive substances[J].Fish&Shellfish Immunology,2018,74:436-443.DOI:10.1016/j.fsi.2018.01.002.
[26]VOWINCKEL J,CAPUANO F,CAMPBELL K,et al.The beauty of being(label)-free:sample preparation methods for SWATH-MS and next-generation targeted proteomics[J].F1000 Research,2013,2:272.DOI:10.12688/f1000research.2-272.v1.
[27]赵小梅,刘歆颖,续畅,等.基于LC-MS代谢组学的雷公藤多苷致肝毒性生物标志物的初步筛查[J].中国中药杂志,2015,40(19):3851-3858.
[28]牟菲.丹参-降香对大鼠心肌缺血/再灌注损伤的保护作用及机制的代谢组学研究[D].西安:第四军医大学,2017.
[29]YU Q,WU W,TIAN X,et al.Unraveling proteome changes of Holstein beef M.semitendinosus and its relationship to meat discoloration during post-mortem storage analyzed by label-free mass spectrometry[J].Journal of Proteomics,2017,154:85-93.DOI:10.1016/j.jprot.2016.12.012.
[30]WIKLUND S,JOHANSSON E,SJ諷TR諱L,et al.Visualization of GC/TOF-MS-based metabolomics data for identification of biochemically interesting compounds using OPLS class models[J].Analytical Chemistry,2008,80(1):115-122.DOI:10.1021/ac0713510.