夏天无注射液对脑缺血再灌注大鼠海马Ang-2和VEGF mRNA表达的影响
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摘要
目的观察夏天无注射液对脑缺血/再灌注损伤大鼠的保护作用,并分析可能的作用机制。方法制备大鼠大脑中动脉阻塞模型,脑缺血2 h/再灌22 h,进行神经功能评分;应用氯化三苯四氮唑染色测量脑组织梗死面积;RTreal-time PCR检测夏天无注射液对大鼠脑缺血/再灌后海马组织血管生成素-2和血管内皮生长因子相对定量水平。结果缺血/再灌注损伤后,与假手术组比较,模型组大鼠神经功能评分明显增高,并有大面积的脑梗死;与模型组比较,夏天无注射液中、高剂量组(0.025 mL/kg、0.05 mL/kg)明显降低神经功能评分、减小脑梗死面积(P<0.05或P<0.01),同时进一步增加缺血区海马组织中Ang-2、VEGF mRNA的表达量(P<0.05)。结论夏天无注射液对大鼠脑缺血/再灌注损伤具有明显保护作用,其增加海马内Ang-2、VEGF表达的作用可能参与了血管新生的早期机制。
引文
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[17]Zhang Z G,Zhang L,Tsang W,et al.Correlation of VEGF andangiopoietin expression with disruption of blood-brain barrier andAngiogenesis after focal cerebral schemia[J].J Cereb BloodFlow Metab,2002,22(4):379-392.
[18]Beck H,Acker T,Wiessner C,et al.Expression of angiopoie-tin-1,angiopoietin-2,and tie receptors after middle cerebral ar-tery occlusion in the rat[J].Am J Pathol,2000,157(5):1473-1483.
[2]胡雪勇,孙安盛,余丽梅,等.夏天无总碱抗实验性脑缺血的作用[J].中西医结合学报,2005,3(1):46-49.
[3]余丽梅,文国容,邓江,等.夏天无注射液对小鼠脑缺血和神经元保护作用的实验研究[J].上海中医药杂志,2006,40(9):70-72.
[4]余丽梅,于爽,龚其海,等.夏天无注射液对血管性痴呆大鼠海马血管生成素-1表达的影响[J].中成药,2006,28(6):839-842.
[5]Yang Y R,Wang R Y,Wang P S.Early and late treadmilltraining after focal Brain ischemia in rats[J].Neurosci Lett,2003,339(2):91-94.
[6]Wen T C,Regido M,Gressons P,et al.A reproducible experi-mental model of focal cerebral ischemia in the neonatal rat[J].Brain Res Brain Res Protoc,2004,13(2):76-83.
[7]Szpak G M,Lechowicz W,Lewandowska E,et al.Border zoneneovascularization in cerebral ischemic infarct[J].Folia Neuro-pathol,1999,37(4):264-268.
[8]Yancopoulos G D,Davis S,Gate N W,et al.Vascular-specificgrowth factors and blood vessel formation[J].Nature,2000,407(6801):242-248.
[9]王海东,王明荣,景涛,等.血管内皮生长因子及其受体在食管癌中的表达和临床意义[J].中国现代医学杂志,2000,10(10):19-20.
[10]Man S M,Zhang D P,Zhang P Y,et al.Investigation of rela-tion between plasma vascular endothelial growth factor(VEGF)and brain vascular disease[J].China J Mod Med,2004,14(10):36-39.Chinese.
[11]Sundberg C,Kowanetz M,Brown L F,et al.Stable expressionof angiopoietin-l and other markers by cultured pericytes:pheno-typic similarities to a subpopulation of cells in maturing vesselsduring later stages of anginge-nesis in vivo[J].Lab Invest.2002,82(4):387-401.
[12]Ardelt A A,McCullough L D,Korach K S,et al.Estradiol reg-ulates angiopoietin-1 mRNA expression through estrogen recep-tor-alpha in a rodent experimental stroke model[J].Stroke,2005,36(2):337-341.
[13]Maisonpierne P C,Suri C,Jones P F,et al.Angiopoietin-2,anatural antagonist for Tie-2 that disrupts in vivo angiogenesis[J].Science,1997,277(5322):55-60.
[14]Lobov I B,Brooks P C,Lang R A.Angiopoietin-2 displaysVEGF-dependent modulation of capillary structure and endotheli-al cell survival in vivo[J].Proc Natl Acad Sci USA,2002,99(17):11205-11210.
[15]Cai M,Zhang H,Hui R.Single chain Fv antibody against an-giopoietin-2 inhibits VEGF-induced endothelial cell proliferationand migration in vitro[J].Biochem Biophys Res Commun,2003,309(4):946-951.
[16]Croll S D,Wiegand S J,Vascular growth factors in cerebral is-chemia[J].Mol Neurobiol,2001,23(2-3):121-135.
[17]Zhang Z G,Zhang L,Tsang W,et al.Correlation of VEGF andangiopoietin expression with disruption of blood-brain barrier andAngiogenesis after focal cerebral schemia[J].J Cereb BloodFlow Metab,2002,22(4):379-392.
[18]Beck H,Acker T,Wiessner C,et al.Expression of angiopoie-tin-1,angiopoietin-2,and tie receptors after middle cerebral ar-tery occlusion in the rat[J].Am J Pathol,2000,157(5):1473-1483.